BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0024.p (209 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value yfbR [R] COG1896 Predicted hydrolases of HD superfamily 57 2e-08 >yfbR [R] COG1896 Predicted hydrolases of HD superfamily Length = 199 Score = 57.0 bits (136), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 40/151 (26%), Positives = 72/151 (47%), Gaps = 2/151 (1%) Query: 6 YFRSLNELERIIRCPGKFKFEEHSVAAHSWKVVQYAKTLADIEEQN-GAVIDWKKLYEIT 64 +F L+ L+ I R P +V+ HS +V A LA I+ + G ++ +++ + Sbjct: 6 FFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHALAAIKNRKFGGNVNAERIALLA 65 Query: 65 SSHDYGEIFIGDIKTPVKHSSLQLRSLIQQVEEGMIDNFIKEHIPEEFKSIFYHQLREGK 124 HD E+ GD+ TPVK+ + Q+ + +E+ + + +PEE + IF + E Sbjct: 66 MYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQKLV-DMVPEELRDIFAPLIDEHA 124 Query: 125 DESLEGLILEVADKMDQVYEAFAELQRGNTE 155 E +++ AD + + EL GN E Sbjct: 125 YSDEEKSLVKQADALCAYLKCLEELAAGNNE 155 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,692,530 Number of extensions: 568302 Number of successful extensions: 1838 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 1838 Number of HSP's successfully gapped: 1 Length of query: 209 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 107 Effective length of database: 26,614,363 Effective search space: 2847736841 Effective search space used: 2847736841 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)