BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0015.p (450 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1128 [R] COG0733 Na+-dependent transporters of the SNF family 265 1e-70 NMB1707 [R] COG0733 Na+-dependent transporters of the SNF family 258 1e-68 NMA1961 [R] COG0733 Na+-dependent transporters of the SNF family 256 4e-68 BS_yocR [R] COG0733 Na+-dependent transporters of the SNF family 253 5e-67 BH0217 [R] COG0733 Na+-dependent transporters of the SNF family 236 7e-62 >BH1128 [R] COG0733 Na+-dependent transporters of the SNF family Length = 453 Score = 265 bits (676), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 162/439 (36%), Positives = 236/439 (53%), Gaps = 9/439 (2%) Query: 15 KERFSSS-GFIFAAIGSSVGLGNMWKFPYITGENGGAAXXXXXXXXXXXXXXPVLLAELA 73 +E+++S GFI AA+GS+VGLGN+W+F Y+TGENGGAA P++LAE Sbjct: 9 REQWASRLGFILAAMGSAVGLGNIWRFSYVTGENGGAAFLLVYLGFIALIGIPIVLAEFT 68 Query: 74 IGRSGRGSAATSFIRXXXXXXXXXXXILQVVAPFLILSFYIIVAGWTLNYALIAFNGQLF 133 IGR + A SF + ++ V A FLILSFY ++AGW L Y GQL+ Sbjct: 69 IGRRAQSDAVGSFEKLAPGKPWKVAGLMGVAAGFLILSFYGVIAGWILFYLFNYITGQLW 128 Query: 134 ST---SDYAGQFGSFISGYMPIVWQGVAMLITAGVVILGVSGGIEKFNKXXXXXXXXXXX 190 S G + +P+ WQ + M+ T +V +GV GIE+ NK Sbjct: 129 SAPAEGFGGFFEGFIANPTLPLFWQALFMIATIWIVAIGVKKGIERSNKILMPLLGVLLI 188 Query: 191 XXXXXXXTLPGSGEGVSFFLKPDFSVLTAESA-LVALGHAFFSLSLGMGILLTYGSYVDK 249 TL G+ EG++F PD+S L L A+ AFF+LSLGMG L+TYGSYV K Sbjct: 189 ALAIYSLTLGGAKEGLAFLFSPDWSALKDPGVYLAAISQAFFTLSLGMGALITYGSYVSK 248 Query: 250 RQXXXXXXXXXXXXDLVYAFIAGLIIFPTTFSFGIAPDQGPSLIFIALPAAFSAMPXXXX 309 D +A IAG++IFP F+ G++P GP L+F+ LP F ++ Sbjct: 249 DSRLPGAAVSVAGLDTAFAIIAGIMIFPAVFALGLSPSGGPGLVFVVLPDIFDSIRLGPI 308 Query: 310 XXXXXXXXXXXXXXXXXXXXXEVPVSFAMERWNWSRRRAVWILSLICFLLGIPSAMSLGM 369 EVPV++ M +++WSR++A L +I LLGIPS++S G+ Sbjct: 309 VGIAFFILLGAAALSSAVSLLEVPVAYFMRKFDWSRKQAAITLGVIITLLGIPSSLSFGV 368 Query: 370 FPELT-FGGKALFDWMDFITSNIMLPLGGLLITIFAGYFWKGA---AEAAGLQARWFRIW 425 E+T G +FD +DFI S++ LPLGG++I +F G+ WK + AE+ + W ++W Sbjct: 369 LGEVTIIPGLNIFDSVDFIASSVFLPLGGMIIALFIGWGWKTSDALAESDLTDSVWGKLW 428 Query: 426 LFMLRYVAPVLVFLVLLHT 444 + LR++AP+ + +V L Sbjct: 429 ILSLRFIAPIAILIVFLSA 447 >NMB1707 [R] COG0733 Na+-dependent transporters of the SNF family Length = 445 Score = 258 bits (659), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 155/445 (34%), Positives = 229/445 (51%), Gaps = 7/445 (1%) Query: 11 LSEKKERFSSSGFIFAAIGSSVGLGNMWKFPYITGENGGAAXXXXXXXXXXXXXXPVLLA 70 +S S GF+ AA GS++GLG +WKFPY G NGGA PV LA Sbjct: 1 MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFLLFLIFTILVALPVQLA 60 Query: 71 ELAIGRSGRGSAATSFIRXXXXXXXXXXXILQVVAPFLILSFYIIVAGWTLNYALIAFNG 130 E IGR+G +A SF + V A F++LSFY +V GW LNY + +F G Sbjct: 61 EFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSFYSVVGGWVLNYVVHSFTG 120 Query: 131 QLFSTSDYAGQFGSFISGYM-PIVWQGVAMLITAGVVILGVSGGIEKFNKXXXXXXXXXX 189 + + +D+ FG+ IS + +Q + MLIT VV G+S GIEK N+ Sbjct: 121 AVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGGISDGIEKANRYLMPGLFILF 180 Query: 190 XXXXXXXXTLPGSGEGVSFFLKPDFSVLTAESALVALGHAFFSLSLGMGILLTYGSYVDK 249 TLPG+ EGVSF LKP++S A++ + ALG AFF+LS+G+ ++TY SY+ K Sbjct: 181 IALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALGQAFFALSIGVSAMITYASYLGK 240 Query: 250 RQXXXXXXXXXXXXDLVYAFIAGLIIFPTTFSFGIAPDQGPSLIFIALPAAFSAMPXXXX 309 Q +L+ + +AGL+IFP F+FG P QGP LIFI LPA F MP Sbjct: 241 DQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFEPSQGPGLIFIVLPAVFMKMPFGTV 300 Query: 310 XXXXXXXXXXXXXXXXXXXXXEVPVSFAMERWNWSRRRAVWILSLICFLLGIPSAMSLGM 369 E ++ + + R++ W++ F++GIPSA+S G+ Sbjct: 301 LFAVFMLLVVFATLTSAFSMLETVIASTIRQDERKRKKHTWLIGTAIFIIGIPSALSFGV 360 Query: 370 FPELTFGGKALFDWMDFITSNIMLPLGGLLITIFAGYFW------KGAAEAAGLQARWFR 423 + E GK +FD D++ S +++P+G L ++IF + K A + + Sbjct: 361 WGEFKVFGKTIFDLWDYVISAVIMPIGALSVSIFTAWIQDKQSVLKDAGAGSTVPRAVLL 420 Query: 424 IWLFMLRYVAPVLVFLVLLHTSGIL 448 +WL LRY+AP+ + +V +++ IL Sbjct: 421 LWLNTLRYLAPIAIIIVFINSLDIL 445 >NMA1961 [R] COG0733 Na+-dependent transporters of the SNF family Length = 348 Score = 256 bits (655), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 0/445 (0%), Positives = 0/445 (0%), Gaps = 7/445 (1%) Query: 11 LSEKKERFSSSGFIFAAIGSSVGLGNMWKFPYITGENGGAAXXXXXXXXXXXXXXPVLLA 70 ++ G + + ++V + Sbjct: 1 MNAFLIFLFILGSVTISTAAAVISVDSNGGGNYISIQEAVNNAQEGDTILVNPGIYKENV 60 Query: 71 ELAIGRSGRGSAATSFIRXXXXXXXXXXXILQVVAPFLILSFYIIVAGWTLNYALIAFNG 130 ++ S ++++ ++ + G Sbjct: 61 KVEKKVSILANSSSEAPGKRIYVLGTVLDEEVFYVNSSNVTIDGFFISGVSSGGDKYEVG 120 Query: 131 QLFSTSDYAGQFGSFISGYM-PIVWQGVAMLITAGVVILGVSGGIEKFNKXXXXXXXXXX 189 + + + I G ++ GI N Sbjct: 121 IYLEGVENCSLSNNALIMNDVGISLTGAKENYLVNNLVSLGYQGIALENSEDNTLLGNLM 180 Query: 190 XXXXXXXXTLPGSGEGVSFFLKPDFSVLTAESALVALGHAFFSLSLGMGILLTYGSYVDK 249 + + + + A ++S + T S + Sbjct: 181 LTNDMGILLYNSANNTLMNNTADSNTKGVSLEASDMNALTSNTISKNEYGIFTLKSKSNV 240 Query: 250 RQXXXXXXXXXXXXDLVYAFIAGLIIFPTTFSFGIAPDQGPSLIFIALPAAFSAMPXXXX 309 + + + Sbjct: 241 LFNNSLYLNGVGAYFNDSSDNTVYMNDFVNYINAEDEGTNFWNTSSTGNYWDKYSGDDAN 300 Query: 310 XXXXXXXXXXXXXXXXXXXXXEVPVSFAMERWNWSRRRAVWILSLICFLLGIPSAMSLGM 369 Sbjct: 301 360 Query: 370 FPELTFGGKALFDWMDFITSNIMLPLGGLLITIFAGYFW------KGAAEAAGLQARWFR 423 Sbjct: 361 420 Query: 424 IWLFMLRYVAPVLVFLVLLHTSGIL 448 Sbjct: 421 445 >BS_yocR [R] COG0733 Na+-dependent transporters of the SNF family Length = 445 Score = 253 bits (645), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 150/444 (33%), Positives = 229/444 (51%), Gaps = 7/444 (1%) Query: 11 LSEKKERFSSSGFIFAAIGSSVGLGNMWKFPYITGENGGAAXXXXXXXXXXXXXXPVLLA 70 + E + S GFI AA GS++GLG +WKFPY+ G +GG A P+LL Sbjct: 1 MKEANQWSSKLGFILAAAGSAIGLGAIWKFPYVAGTSGGGAFLLIFILFTVLIGLPLLLG 60 Query: 71 ELAIGRSGRGSAATSFIRXXXXXXXXXXXILQVVAPFLILSFYIIVAGWTLNYALIAFNG 130 E IGR + A S+ L +V F++LSFY +V GW L Y + F G Sbjct: 61 EFIIGRRTQKDAVQSYKTLAPGTKWHWIGYLGMVTCFILLSFYSVVGGWILIYIIKGFTG 120 Query: 131 QLFSTSDYAGQFGSFISG-YMPIVWQGVAMLITAGVVILGVSGGIEKFNKXXXXXXXXXX 189 L TS + F S IS Y+ + Q + M++T VV GVS GIEK ++ Sbjct: 121 GLSQTSGFDTIFASTISNPYLAVGGQLLFMVVTILVVAKGVSSGIEKASQVLMPALFLLF 180 Query: 190 XXXXXXXXTLPGSGEGVSFFLKPDFSVLTAESALVALGHAFFSLSLGMGILLTYGSYVDK 249 TL G+ EG+ FFL PD + + A + L A+G +FF LS+G+ +++TY +Y++K Sbjct: 181 IILMVRSVTLDGAMEGIKFFLMPDLTAIDANTILYAMGQSFFLLSVGVSVMVTYSAYLEK 240 Query: 250 RQXXXXXXXXXXXXDLVYAFIAGLIIFPTTFSFGIAPDQGPSLIFIALPAAFSAMPXXXX 309 ++ +++ A +AG+ IFP FSFG+ PDQGP L+F LP F+ MP Sbjct: 241 QENLVQSAVSVTVMNMLVAVMAGIAIFPAVFSFGLKPDQGPVLLFNVLPTVFNQMPFGII 300 Query: 310 XXXXXXXXXXXXXXXXXXXXXEVPVSFAMERWNWSRRRAVWILSLICFLLGIPSAMSLGM 369 E+ V+ + R+R W+ + F++G+PSA+S G+ Sbjct: 301 FLLAFLLLFLFAALTSAFSMLEILVAVLSKGDINKRKRFAWLGGIAIFIVGVPSALSYGV 360 Query: 370 FPELTFGGKALFDWMDFITSNIMLPLGGLLITIFAGY------FWKGAAEAAGLQARWFR 423 ++T ++FD D++ SNI++PLG LLI+IF + + L+ +WF Sbjct: 361 LSDVTIFHLSIFDAADYLVSNILMPLGALLISIFVPLKIPKQDLFDELKSGSNLKRKWFA 420 Query: 424 IWLFMLRYVAPVLVFLVLLHTSGI 447 +WL ++RY++PV + +V LH GI Sbjct: 421 VWLLLIRYLSPVAIIIVFLHVIGI 444 >BH0217 [R] COG0733 Na+-dependent transporters of the SNF family Length = 457 Score = 236 bits (601), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 156/447 (34%), Positives = 231/447 (51%), Gaps = 12/447 (2%) Query: 15 KERFSSS-GFIFAAIGSSVGLGNMWKFPYITGENGGAAXXXXXXXXXXXXXXPVLLAELA 73 K+++SS FI AA GS+VGLG +WKFPY+TG +GG A P+L+ E Sbjct: 2 KQQWSSKLAFILAAAGSAVGLGAIWKFPYVTGTSGGGAFFLIFILFTLLIGLPLLIGEFV 61 Query: 74 IGRSGRGSAATSFIRXXXXXXXXXXXILQVVAPFLILSFYIIVAGWTLNYALIAFNGQL- 132 IGRS + +A S+ L + FL+LSFY ++ GW + Y L A GQL Sbjct: 62 IGRSTQKNAIESYRAIAPNSSWGGVGKLGIFTCFLLLSFYSVIGGWIIIYFLKAITGQLS 121 Query: 133 -FSTSDYAGQFGSFISG-YMPIVWQGVAMLITAGVVILGVSGGIEKFNKXXXXXXXXXXX 190 Y F IS + I Q + I VV GV GIE+ + Sbjct: 122 GLDEGRYGEIFAETISNPILAISSQFAFLAIVMYVVSRGVKKGIERASVIMMPALFVLFL 181 Query: 191 XXXXXXXTLPGSGEGVSFFLKPDFSVLTAESALVALGHAFFSLSLGMGILLTYGSYVDKR 250 TL G+ EG++FFL PDFS +T+E+ L A+G +FF+LS+G+ +++TY SY+ + Sbjct: 182 AIVIRGLTLDGATEGLAFFLVPDFSTITSETLLYAMGQSFFALSVGVSVMVTYSSYLSRD 241 Query: 251 QXXXXXXXXXXXXDLVYAFIAGLIIFPTTFSFGIAPDQGPSLIFIALPAAFSAMPXXXXX 310 + V AF+AG+ IFP FSFG++PD+GP L+F LP+ F MP Sbjct: 242 ESIVQSALSIVMLTFVVAFLAGVAIFPAVFSFGMSPDEGPVLLFNVLPSVFDQMPYGAFF 301 Query: 311 XXXXXXXXXXXXXXXXXXXXEVPVSFAMERWNWSRR-RAVWILSLICFLLGIPSAMSLGM 369 EV V+ A+ + + S+R + W++ L F +GIPSA+S G+ Sbjct: 302 LVTFLLLFLFATLTSAFSMLEVVVA-AITKGDVSQRPKVTWLIGLAIFAVGIPSALSYGV 360 Query: 370 FPELTFGGKALFDWMDFITSNIMLPLGGLLITIFA------GYFWKGAAEAAGLQARWFR 423 + ++ G+ +FD DF+ SNI++P+G LLI IF ++ A + R F Sbjct: 361 WSDVLLFGRTVFDTADFLVSNILMPIGALLIAIFVPLKISKERLYEEIKAGASIGKRLFA 420 Query: 424 IWLFMLRYVAPVLVFLVLLHTSGILTF 450 IW ++RYVAP+++ +V L G L+F Sbjct: 421 IWFILIRYVAPIVIAIVFLDVIGALSF 447 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.143 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,492,259 Number of extensions: 937002 Number of successful extensions: 2425 Number of sequences better than 1.0e-05: 31 Number of HSP's gapped: 2288 Number of HSP's successfully gapped: 31 Length of query: 450 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 340 Effective length of database: 25,691,691 Effective search space: 8735174940 Effective search space used: 8735174940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)