BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0011.p (233 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1285 [K] COG1191 DNA-directed RNA polymerase specialized sigma... 345 3e-95 CAC1689 [K] COG1191 DNA-directed RNA polymerase specialized sigm... 250 1e-66 BH2556 [K] COG1191 DNA-directed RNA polymerase specialized sigma... 211 8e-55 BS_sigE [K] COG1191 DNA-directed RNA polymerase specialized sigm... 205 5e-53 CAC1695 [K] COG1191 DNA-directed RNA polymerase specialized sigm... 195 6e-50 >BH1285 [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit Length = 235 Score = 345 bits (885), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 169/230 (73%), Positives = 196/230 (85%), Gaps = 1/230 (0%) Query: 1 LPGIISTIALLIKELTLLVSYVRNNAFPQPLSEQDESKYLGRMAEGDAKARNLLIEHNLR 60 + II+ + KE+ + VSYV+NNAFPQPLS+++E YL RMAEGD +ARN LIEHNLR Sbjct: 1 MSSIIAALTYFFKEVLVFVSYVKNNAFPQPLSKEEERHYLKRMAEGDEEARNRLIEHNLR 60 Query: 61 LVAHIVKKFDNTGEDMEDLISIGTIGLIKAIESYRPNKGTKLATFAARCIENEILMHLRS 120 LVAHIVKKF+NT E EDLISIGTIGLIKAIES+ KGTKLAT+AARCIENEILMHLR+ Sbjct: 61 LVAHIVKKFENTREHTEDLISIGTIGLIKAIESFSEGKGTKLATYAARCIENEILMHLRA 120 Query: 121 LKKTRKDVSLHDPIGTDKEGNEITLIDILGSETDDVIKEVDLKIEKSKIYRNLDILDDRE 180 LKK +KDVSLHDPIGTDKEGNEITLID+L +++D+ + LK+EK +IY + +LDDRE Sbjct: 121 LKKVKKDVSLHDPIGTDKEGNEITLIDVLQEDSEDIADSIQLKMEKKQIYEYIHVLDDRE 180 Query: 181 KEVVVGRFGLDTGGEERTQREIAKELGISRSYVSRIEKRALMKLYHEFYK 230 KEV+VGRFGLD EERTQREIAKELGISRSYVSRIEKRALMKL+HEFYK Sbjct: 181 KEVIVGRFGLDL-EEERTQREIAKELGISRSYVSRIEKRALMKLFHEFYK 229 >CAC1689 [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit Length = 234 Score = 250 bits (639), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 130/220 (59%), Positives = 171/220 (77%), Gaps = 3/220 (1%) Query: 11 LIKELTLLVSYVRNNA-FPQPLSEQDESKYLGRMAEGDAKARNLLIEHNLRLVAHIVKKF 69 ++ +T V+Y+ N A FPQPLSE +E YL ++ EGD A+N+LIE NLRLVAHIVKK+ Sbjct: 10 IVGSITFFVAYIGNGASFPQPLSESEEKYYLKKLKEGDPLAKNILIERNLRLVAHIVKKY 69 Query: 70 DNTGEDMEDLISIGTIGLIKAIESYRPNKGTKLATFAARCIENEILMHLRSLKKTRKDVS 129 +G++++DLISIGTIGLIKAI+SY +KGT+LAT+AARCIENEILM +R+ KK++ +V Sbjct: 70 SYSGKEVDDLISIGTIGLIKAIDSYNVSKGTRLATYAARCIENEILMLIRNNKKSKNEVY 129 Query: 130 LHDPIGTDKEGNEITLIDILGSETDDVIKEVDLKIEKSKIYRNLD-ILDDREKEVVVGRF 188 L DPIG DKEGNEI+LIDIL S+ D VI+ V+ +I+ K+Y ++ +L REK ++ R+ Sbjct: 130 LQDPIGVDKEGNEISLIDILSSDDDSVIEIVENRIQIGKLYVEINRVLKGREKAIIKMRY 189 Query: 189 GLDTGGEERTQREIAKELGISRSYVSRIEKRALMKLYHEF 228 GL G+ R QREIA+ LGISRSYVSRIEKRAL KLY + Sbjct: 190 GLGD-GKPRNQREIAQILGISRSYVSRIEKRALKKLYRQL 228 >BH2556 [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit Length = 237 Score = 211 bits (537), Expect = 8e-55, Method: Compositional matrix adjust. Identities = 103/205 (50%), Positives = 151/205 (73%), Gaps = 1/205 (0%) Query: 26 AFPQPLSEQDESKYLGRMAEGDAKARNLLIEHNLRLVAHIVKKFDNTGEDMEDLISIGTI 85 A P PLS+++E+ L ++ GD R++LIE NLRLV +I +KF+NTG ++EDLISIGTI Sbjct: 32 ALPPPLSKEEEAHLLKKLPSGDKAVRSMLIERNLRLVVYIARKFENTGINIEDLISIGTI 91 Query: 86 GLIKAIESYRPNKGTKLATFAARCIENEILMHLRSLKKTRKDVSLHDPIGTDKEGNEITL 145 GLIKA+ ++ P K KLAT+A+RCIENEILM+LR K R +VS +P+ D +GNE+ L Sbjct: 92 GLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKIRSEVSFDEPLNIDWDGNELLL 151 Query: 146 IDILGSETDDVIKEVDLKIEKSKIYRNLDILDDREKEVVVGRFGLDTGGEERTQREIAKE 205 D+LG++ D + + ++ K+++ + + L L DREK+++ RFGL GGEE+TQ+++A Sbjct: 152 SDVLGTDDDIITRGIEEKVDRKLLMKALHTLTDREKQIMELRFGL-AGGEEKTQKDVADL 210 Query: 206 LGISRSYVSRIEKRALMKLYHEFYK 230 LGIS+SY+SR+EKR + +L EF K Sbjct: 211 LGISQSYISRLEKRIIKRLQKEFNK 235 >BS_sigE [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit Length = 239 Score = 205 bits (521), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 101/205 (49%), Positives = 150/205 (73%), Gaps = 1/205 (0%) Query: 26 AFPQPLSEQDESKYLGRMAEGDAKARNLLIEHNLRLVAHIVKKFDNTGEDMEDLISIGTI 85 A P PLS+ +E L ++ GD AR +LIE LRLV +I +KF+NTG ++EDLISIGTI Sbjct: 34 ALPPPLSKDEEQVLLMKLPNGDQAARAILIERILRLVVYIARKFENTGINIEDLISIGTI 93 Query: 86 GLIKAIESYRPNKGTKLATFAARCIENEILMHLRSLKKTRKDVSLHDPIGTDKEGNEITL 145 GLIKA+ ++ P K KLAT+A+RCIENEILM+LR K R +VS ++P+ D +GNE+ L Sbjct: 94 GLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKIRSEVSFYEPLNIDWDGNELLL 153 Query: 146 IDILGSETDDVIKEVDLKIEKSKIYRNLDILDDREKEVVVGRFGLDTGGEERTQREIAKE 205 D+LG++ D + K+++ ++K + + L+ L++REK+++ RFGL G EE+TQ+++A Sbjct: 154 SDVLGTDDDIITKDIEANVDKKLLKKALEQLNEREKQIMELRFGL-VGEEEKTQKDVADM 212 Query: 206 LGISRSYVSRIEKRALMKLYHEFYK 230 +GIS+SY+SR+EKR + +L EF K Sbjct: 213 MGISQSYISRLEKRIIKRLRKEFNK 237 >CAC1695 [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit Length = 235 Score = 195 bits (495), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 98/207 (47%), Positives = 146/207 (70%), Gaps = 1/207 (0%) Query: 24 NNAFPQPLSEQDESKYLGRMAEGDAKARNLLIEHNLRLVAHIVKKFDNTGEDMEDLISIG 83 N+A P PLS+ +E + ++ GD R++LIE NLRLV +I +KF+NTG +EDLIS+G Sbjct: 28 NDALPPPLSKDEEDDLVNKLVNGDESIRSILIERNLRLVVYIARKFENTGVGVEDLISVG 87 Query: 84 TIGLIKAIESYRPNKGTKLATFAARCIENEILMHLRSLKKTRKDVSLHDPIGTDKEGNEI 143 TIGLIKA+ ++ P K KLAT+ +RCIENEILM+LR K + ++S ++P+ D +GN++ Sbjct: 88 TIGLIKAVNTFDPTKKIKLATYGSRCIENEILMYLRRNSKVKAEISFYEPLNIDWDGNKL 147 Query: 144 TLIDILGSETDDVIKEVDLKIEKSKIYRNLDILDDREKEVVVGRFGLDTGGEERTQREIA 203 L DILG++ D V ++ +++K + L L++REK +V R+GL TG E+TQ+E+A Sbjct: 148 LLSDILGTDNDCVYNLIEGEVDKQLLLFALKKLNEREKRIVELRYGL-TGVGEKTQKEVA 206 Query: 204 KELGISRSYVSRIEKRALMKLYHEFYK 230 LGIS+SY+SR+EKR + +L E K Sbjct: 207 DMLGISQSYISRLEKRIIKRLKKEINK 233 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.137 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,242,568 Number of extensions: 742935 Number of successful extensions: 2073 Number of sequences better than 1.0e-05: 106 Number of HSP's gapped: 2043 Number of HSP's successfully gapped: 119 Length of query: 233 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 130 Effective length of database: 26,499,029 Effective search space: 3444873770 Effective search space used: 3444873770 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)