BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0462.0011.p (347 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yhfE [G] COG1363 Cellulase M and related proteins 332 6e-91 SA1198 [G] COG1363 Cellulase M and related proteins 327 1e-89 lin0981 [G] COG1363 Cellulase M and related proteins 325 5e-89 CAC0690 [G] COG1363 Cellulase M and related proteins 323 3e-88 PH0737 [G] COG1363 Cellulase M and related proteins 305 8e-83 >BS_yhfE [G] COG1363 Cellulase M and related proteins Length = 346 Score = 332 bits (850), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 170/334 (50%), Positives = 224/334 (67%), Gaps = 1/334 (0%) Query: 12 LSVLKKLLDTPSPSGFTAQVMKLVAEEAAALGIPLSWNEKGGAILTVPGLDPARTIGISA 71 + ++K+L+ PSP+G T +V+ + + N KGG I T+PG D +R ++A Sbjct: 8 MELIKELVSIPSPTGNTYEVINYIESLLKEWKVETVRNHKGGLIATLPGRDTSRHRMLTA 67 Query: 72 HVDTLGAMVRSIKPNGTLRLTSVGGFTMNSIENEYCVIHTRTGLTYTGTILSSHPSVHVY 131 HVDTLGAMV+ IK +G L++ +GGF NSIE EYC I T +G YTGTIL SVHVY Sbjct: 68 HVDTLGAMVKEIKADGRLKIDLIGGFRYNSIEGEYCQIETASGKMYTGTILMHQTSVHVY 127 Query: 132 ADARDFKRSEENMEIRIDEVVSTKDDVLKLGIAVGDFISFDARAVITPSGYIKSRHLDDK 191 DA +R++ENMEIR+DE V + D +LGI VGDF+SFD R IT SG+IKSRHLDDK Sbjct: 128 KDAGKAERNQENMEIRLDEPVHCRKDTEELGIGVGDFVSFDPRVEITSSGFIKSRHLDDK 187 Query: 192 ASVAALLGLLESMKREGWKPLHNLSLLISNYEEVGHGA-AWIPGGISEMIAVDMGAMGDD 250 ASVA LL L+ ++ E + + LISN EE+G+G + IP E +AVDMGA+GD Sbjct: 188 ASVALLLRLIHEIQTEDIELPYTTHFLISNNEEIGYGGNSNIPPETVEYLAVDMGAIGDG 247 Query: 251 LSCKETDVSICAKDSSGPYDYAMTSKLIELANGLAIPFAVDIYPQYGSDASAALRGGNNI 310 + E VSIC KD+SGPY Y + L++LA I + +DIYP YGSDASAA++ G++I Sbjct: 248 QATDEYSVSICVKDASGPYHYQLRKHLVQLAEKHHIDYKLDIYPYYGSDASAAIKSGHDI 307 Query: 311 RAALIGPGVHASHSMERTHKQAVLNTAKLLAAYV 344 LIGPG+ ASH+ ERTHK ++ +TAKLL YV Sbjct: 308 VHGLIGPGIDASHAFERTHKSSLRHTAKLLYYYV 341 >SA1198 [G] COG1363 Cellulase M and related proteins Length = 343 Score = 327 bits (839), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 172/335 (51%), Positives = 218/335 (65%), Gaps = 1/335 (0%) Query: 11 ILSVLKKLLDTPSPSGFTAQVMKLVAEEAAALGIPLSWNEKGGAILTVPGLDPARTIGIS 70 IL ++K L SPSG T + ++ V + A LG + KG ++TVPG + I+ Sbjct: 4 ILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNKGALLITVPGKNDEVQRCIT 63 Query: 71 AHVDTLGAMVRSIKPNGTLRLTSVGGFTMNSIENEYCVIHTRTGLTYTGTILSSHPSVHV 130 AHVDTLGAMV+ IK +G L + +GGFT N+IE EYC I T G YTGTI SVHV Sbjct: 64 AHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQIKTDAGQIYTGTICLHETSVHV 123 Query: 131 YADARDFKRSEENMEIRIDEVVSTKDDVLKLGIAVGDFISFDARAVITPSGYIKSRHLDD 190 Y + + R +++MEIRIDEV ++++D LGI+VGDF+SFD R VIT SG+IKSRHLDD Sbjct: 124 YRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGDFVSFDPRTVITSSGFIKSRHLDD 183 Query: 191 KASVAALLGLLESMKREGWKPLHNLSLLISNYEEVGHGA-AWIPGGISEMIAVDMGAMGD 249 KASVA +L LL+ +K E H ISN EE+G+GA A I I E IA+DMGA+GD Sbjct: 184 KASVAMILQLLKKLKEEQIILPHTTQFYISNNEEIGYGANASIDSKIKEYIALDMGALGD 243 Query: 250 DLSCKETDVSICAKDSSGPYDYAMTSKLIELANGLAIPFAVDIYPQYGSDASAALRGGNN 309 + E VSICAKD+SGPY + S L+ L IP+ VDIYP YGSDASAAL G + Sbjct: 244 GQASDEYTVSICAKDASGPYHKQLKSHLVNLCKINNIPYKVDIYPYYGSDASAALHAGAD 303 Query: 310 IRAALIGPGVHASHSMERTHKQAVLNTAKLLAAYV 344 IR L G G+ +SH+MERTH ++ T KLL AY Sbjct: 304 IRHGLFGAGIESSHAMERTHIDSIKATEKLLYAYC 338 >lin0981 [G] COG1363 Cellulase M and related proteins Length = 350 Score = 325 bits (834), Expect = 5e-89, Method: Compositional matrix adjust. Identities = 168/347 (48%), Positives = 222/347 (63%), Gaps = 1/347 (0%) Query: 2 LTIQPNEAYILSVLKKLLDTPSPSGFTAQVMKLVAEEAAALGIPLSWNEKGGAILTVPGL 61 ++ +PN + +K+L PSP+G T Q+++ + + A IP N KGG I+T+PG Sbjct: 1 MSYEPNTTQTMQKIKELTSIPSPTGNTGQIIEKLVRDLEASNIPYKLNNKGGLIVTLPGK 60 Query: 62 DPARTIGISAHVDTLGAMVRSIKPNGTLRLTSVGGFTMNSIENEYCVIHTRTGLTYTGTI 121 D ++ ++AHVDTLGAMV+ IK +G L LT +GG+ N+IE EYC I T G Y+GTI Sbjct: 61 DDSKHRMLTAHVDTLGAMVKEIKADGRLLLTLIGGYRFNAIEGEYCTIETSEGDLYSGTI 120 Query: 122 LSSHPSVHVYADARDFKRSEENMEIRIDEVVSTKDDVLKLGIAVGDFISFDARAVITPSG 181 L SVHVY DA +R+++NME+R+D D V LGI VGDF+SFD R I Sbjct: 121 LMHQTSVHVYKDAGTAERNDKNMEVRLDVKALDADKVRALGIEVGDFVSFDPRVQIINDE 180 Query: 182 YIKSRHLDDKASVAALLGLLESMKREGWKPLHNLSLLISNYEEVGHGA-AWIPGGISEMI 240 YIKSRHLDDKASVA LL L+ + + H LISN EE+G+G + IP E + Sbjct: 181 YIKSRHLDDKASVAILLQLINHIHENKLELPHTTHFLISNNEEIGYGGNSNIPAETVEYL 240 Query: 241 AVDMGAMGDDLSCKETDVSICAKDSSGPYDYAMTSKLIELANGLAIPFAVDIYPQYGSDA 300 AVDMGA+GD + E VSICAKD SGPY + L+ELA I + VDIYP Y SDA Sbjct: 241 AVDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKKNNIDYKVDIYPFYASDA 300 Query: 301 SAALRGGNNIRAALIGPGVHASHSMERTHKQAVLNTAKLLAAYVGAN 347 SAA+ GN+I LIGPG+ ASH+ ERTH+ ++ +T KL+ AY+ +N Sbjct: 301 SAAINSGNDIVHGLIGPGIDASHAYERTHRDSLYHTEKLVYAYLFSN 347 >CAC0690 [G] COG1363 Cellulase M and related proteins Length = 343 Score = 323 bits (827), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 165/338 (48%), Positives = 212/338 (62%) Query: 7 NEAYILSVLKKLLDTPSPSGFTAQVMKLVAEEAAALGIPLSWNEKGGAILTVPGLDPART 66 N + L+ LL SPSG+T V+ + +E LG+ KG + T+PG + + Sbjct: 3 NSKELTVFLQNLLQINSPSGYTHDVVSYIEKEFCKLGVHYKITNKGNIMATLPGKNSSFE 62 Query: 67 IGISAHVDTLGAMVRSIKPNGTLRLTSVGGFTMNSIENEYCVIHTRTGLTYTGTILSSHP 126 ISAH+DTLGAMV+ IK NGTL LT +GG+ MNSIE E C I T YTGTI S+ P Sbjct: 63 RTISAHLDTLGAMVKEIKSNGTLSLTPIGGYMMNSIEGENCNIMTLDDKKYTGTIQSTKP 122 Query: 127 SVHVYADARDFKRSEENMEIRIDEVVSTKDDVLKLGIAVGDFISFDARAVITPSGYIKSR 186 SVH++ DA++ KR ENME+ IDE V +K+DV LGI VGDFI FD R T SG+IKSR Sbjct: 123 SVHIHNDAKELKRIPENMEVVIDEKVFSKEDVEALGINVGDFICFDPRIKFTDSGFIKSR 182 Query: 187 HLDDKASVAALLGLLESMKREGWKPLHNLSLLISNYEEVGHGAAWIPGGISEMIAVDMGA 246 HLDDKAS A +L ++ + + + S YEEVGHGA+ +P E++ +DMGA Sbjct: 183 HLDDKASAAIMLYAIKHFIDNNIELKYTTNFYFSTYEEVGHGASNLPQNTKELLCIDMGA 242 Query: 247 MGDDLSCKETDVSICAKDSSGPYDYAMTSKLIELANGLAIPFAVDIYPQYGSDASAALRG 306 G + E ICAKDSSGPYDYA +L+++ IP+ +D YP YGSDASAAL+ Sbjct: 243 PGKGQNSSEFATCICAKDSSGPYDYAFRKRLVDICKNKNIPYRIDTYPYYGSDASAALKS 302 Query: 307 GNNIRAALIGPGVHASHSMERTHKQAVLNTAKLLAAYV 344 G +I+ ALIG GV+ASH ERTH +L T LL Y Sbjct: 303 GYDIKTALIGTGVYASHGYERTHLDGMLATLDLLINYC 340 >PH0737 [G] COG1363 Cellulase M and related proteins Length = 336 Score = 305 bits (781), Expect = 8e-83, Method: Compositional matrix adjust. Identities = 160/335 (47%), Positives = 219/335 (65%), Gaps = 7/335 (2%) Query: 11 ILSVLKKLLDTPSPSGFTAQVMKLVAEEAAALGIPLSWNEKGGAILTVPGLDPARTIGIS 70 I+ +L+++L+ PSP+G+T +VM + + + + KG I G P + + Sbjct: 4 IVKILREILEIPSPTGYTKEVMSYLEKFLKENEVNFYYTNKGALI---AGNHPKPELVVI 60 Query: 71 AHVDTLGAMVRSIKPNGTLRLTSVGGFTMNSIENEYCVIHTRTGLTYTGTILSSHPSVHV 130 AHVDTLGAMV+ I P+G L + +GG + + E EYC I TR G + GT+L +PS HV Sbjct: 61 AHVDTLGAMVKEILPDGHLAFSRIGGLVLPTFEGEYCTIITRKGKKFRGTLLLRNPSAHV 120 Query: 131 YADARDFKRSEENMEIRIDEVVSTKDDVLKLGIAVGDFISFDARAVITPSGYIKSRHLDD 190 + +R EENM IR+DE+V ++D KLGI GDFI+FD + +G++KS LDD Sbjct: 121 NREVGKKERKEENMYIRLDELVEKREDTEKLGIRPGDFIAFDPKFEYV-NGFVKSHFLDD 179 Query: 191 KASVAALLGLLESMKREGWKPLHNLSLLISNYEEVGHG-AAWIPGGISEMIAVDMGAMGD 249 KASVAA+L L+ MK E K + ++ S YEEVGHG +A P E++ VDMG +G+ Sbjct: 180 KASVAAILDLIIDMKDELEK--YPVAFFFSPYEEVGHGGSAGYPPTTKELLVVDMGVVGE 237 Query: 250 DLSCKETDVSICAKDSSGPYDYAMTSKLIELANGLAIPFAVDIYPQYGSDASAALRGGNN 309 +S KET VSI AKD++GPYDY MT++LIELA IP+ VD++P YGSD SAALR G + Sbjct: 238 GVSGKETAVSIAAKDTTGPYDYDMTNRLIELAEENNIPYVVDVFPYYGSDGSAALRAGWD 297 Query: 310 IRAALIGPGVHASHSMERTHKQAVLNTAKLLAAYV 344 R ALIGPGVHASH MERTH + +L T +L+ AY+ Sbjct: 298 FRVALIGPGVHASHGMERTHVKGLLATKELIRAYI 332 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,782,290 Number of extensions: 1040468 Number of successful extensions: 2795 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 2763 Number of HSP's successfully gapped: 34 Length of query: 347 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 240 Effective length of database: 26,037,693 Effective search space: 6249046320 Effective search space used: 6249046320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)