BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0462.0004.p (207 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1071 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 222 3e-58 PA0318 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 139 2e-33 BS_ycgM [E] COG0506 Proline dehydrogenase 127 1e-29 STM1812 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 126 2e-29 Z1942 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene... 125 4e-29 >BH1071 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Length = 346 Score = 222 bits (566), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 13/194 (6%), Positives = 36/194 (18%) Query: 4 DVNNIYCVGRNYRQHAEELGNKVPSEPLVFLKPSHAAVPLDKAIIQLPKDAGLIHYEGEI 63 + I + + + E Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 64 VLRIARDYVPGMSVQELVDVMALGLDFTLRDIHNDLQKKGLPWTPAKGFKNAAPLTPYIA 123 ++ + + + + +ND + K + A + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 124 FPETEELEATDYTVRKNGVEVQRGNVKNMIFSLQKIVDFIAARYGLGKDDLIFTGTPAGV 183 + +L + R + G ++D + A + I + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 184 GPVASGDSFELFWG 197 L Sbjct: 182 TAFGVMRYLHLADK 195 >PA0318 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Length = 346 Score = 139 bits (351), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 11/198 (5%), Positives = 21/198 (10%), Gaps = 14/198 (7%) Query: 5 VNNIYCVGRNYRQHAEELGNKVPSEPLVFLKPSHAAVPLDKAIIQLPKDAGLIHYEGEIV 64 G + + L P+ + Sbjct: 17 GLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPR-PETQGLTETYVGNWLAK 75 Query: 65 LRIARDYVPGMSVQELVDVM---ALGLDFTLRDIHNDLQKKGLPWTPAKGFKNAAPLTPY 121 + V R + L P Sbjct: 76 HGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQ 135 Query: 122 I---AFPETEELEATDYTVRK--NGVEVQRGNVKNMIFSLQKIVDFIAARYGLGKDDLIF 176 F + + + KI + Sbjct: 136 RPTNCFG-----KLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYL 190 Query: 177 TGTPAGVGPVASGDSFEL 194 P Sbjct: 191 HLADKHDLPRIVTIQNPY 208 >BS_ycgM [E] COG0506 Proline dehydrogenase Length = 219 Score = 127 bits (318), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 68/200 (34%), Positives = 113/200 (56%), Gaps = 5/200 (2%) Query: 5 VNNIYCVGRNYRQHAEELGNKVPSEPLVFLKPSHAAVPLDKAIIQLPKDAGLIHYEGEIV 64 V+ + CVG NY +H +E+G+ VP EP++F+KP A L + + +P D G +H+E E+ Sbjct: 16 VSKVVCVGSNYAKHIKEMGSAVPEEPVLFIKPETALCDLRQP-LAIPSDFGSVHHEVELA 74 Query: 65 LRIARDYVPGMS--VQELVDVMALGLDFTLRDIHNDLQKKGLPWTPAKGFKNAAPLTPYI 122 + I V++ + + LD TLRD+ ++K G PW AK F N+ PL+ +I Sbjct: 75 VLIGATLRQATEEHVRKAIAGYGVALDLTLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFI 134 Query: 123 AFPE-TEELEATDYTVRKNGVEVQRGNVKNMIFSLQKIVDFIAARYGLGKDDLIFTGTPA 181 E T + + T ++ NG + Q+G +MI + ++ +++ + L D++ TGTP Sbjct: 135 PAAEFTGDPQNTTLSLSVNGEQRQQGTTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPD 194 Query: 182 GVGPVASGDSFEL-FWGETL 200 GVGP+ SGD + F G +L Sbjct: 195 GVGPLQSGDELTVTFDGHSL 214 >STM1812 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Length = 346 Score = 126 bits (317), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 10/200 (5%), Positives = 31/200 (15%), Gaps = 5/200 (2%) Query: 5 VNNIYCVGRNYRQHAEELGNKVPSEPLVFLKPSHAAVPLDKAIIQLPKDAGLIHYEGEIV 64 + A+ + A + + + + Sbjct: 16 LGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPR-PETQGLTETYVGNWLA 74 Query: 65 LRIARDYVPGMS--VQELVDVMALGLDFTLRDIHNDLQKKGLPWTPAKGFKNAAPLTPYI 122 +R+ + S + D N + + + Sbjct: 75 KHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWP 134 Query: 123 AFPE-TEELEATDYTVRKNGVEVQRGNVKNMIFSLQKIVDFIAARYGLGKDDLIFTGTPA 181 P +T V + + + +I + + Sbjct: 135 QRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLAD 194 Query: 182 GVGPVASGDSFEL-FWGETL 200 Sbjct: 195 KHDLPRIVTIQNPYSLLNRS 214 >Z1942 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Length = 346 Score = 125 bits (315), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 10/200 (5%), Positives = 31/200 (15%), Gaps = 5/200 (2%) Query: 5 VNNIYCVGRNYRQHAEELGNKVPSEPLVFLKPSHAAVPLDKAIIQLPKDAGLIHYEGEIV 64 + A+ + A + + + + Sbjct: 16 LGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPP-RPETQGLTETYVGNWLA 74 Query: 65 LRIARDYVPGMS--VQELVDVMALGLDFTLRDIHNDLQKKGLPWTPAKGFKNAAPLTPYI 122 +R+ + S + D N + + + Sbjct: 75 KHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWP 134 Query: 123 AFPE-TEELEATDYTVRKNGVEVQRGNVKNMIFSLQKIVDFIAARYGLGKDDLIFTGTPA 181 P +T V + + + +I + + Sbjct: 135 QRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLAD 194 Query: 182 GVGPVASGDSFEL-FWGETL 200 Sbjct: 195 KHDLPRIVTIQNPYSLLNRS 214 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.140 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,703,767 Number of extensions: 677801 Number of successful extensions: 1317 Number of sequences better than 1.0e-05: 68 Number of HSP's gapped: 1220 Number of HSP's successfully gapped: 68 Length of query: 207 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 105 Effective length of database: 26,614,363 Effective search space: 2794508115 Effective search space used: 2794508115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)