BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0462.0003.p (246 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value FN1891 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 156 2e-38 BS_yhdW [C] COG0584 Glycerophosphoryl diester phosphodiesterase 149 5e-36 BH3700 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 135 5e-32 CAC0430 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 130 1e-30 YPO3792 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 123 3e-28 >FN1891 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 261 Score = 156 bits (395), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 83/234 (35%), Positives = 143/234 (61%), Gaps = 5/234 (2%) Query: 8 CVAHRGFSGKAPENTLAAVRMAIALPFVRWMEIDVQLTKDGVPVVIHDFSLDRTTNGHGK 67 AHRG SG APENTL A++ AI + +EID+QLTKDG VVIHD+ +DRTT G G Sbjct: 24 IFAHRGASGYAPENTLTAIKKAIEMK-ADGIEIDIQLTKDGKIVVIHDWKVDRTTTGRGF 82 Query: 68 VKNMDYEHVRRLDAGSWKGRAFRGERVPSLKEVLELASGRLRLNIELKTSGDMYPGLEQA 127 V +D++++R LDAG W + F GE VP+L+EVL++ + LNIE+K + + +E+ Sbjct: 83 VYELDFDYIRSLDAGQWYTKDFVGEVVPTLEEVLDVLPNDMMLNIEIKDTARKHSNIEEK 142 Query: 128 VIDLVNAHGMR-DEVVLTSFDAGALQRIKELDPRFRTGLIYDSRSGDPARKLKE--LDCS 184 +++++ + + ++++++SF ++R++EL+P+ + L+ DS + + L L Sbjct: 143 MLEVLKKYPEKFEDIIVSSFHHDKIKRLQELEPKLKLALLTDSEFIEIEKYLSTNGLKSY 202 Query: 185 FLSISFDRLNPGLAKLLAERGIRVMAWTVNKAKEMRRLSAMHSEIMICTNRPDI 238 + ++ ++L + ++V WTVNK +++ L + + +I TN PDI Sbjct: 203 SYHPEINLISKKDVEILHKNDVKVFVWTVNKEEDLDYLVTLGVDGVI-TNYPDI 255 >BS_yhdW [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 305 Score = 149 bits (375), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 26/234 (11%), Positives = 48/234 (20%), Gaps = 6/234 (2%) Query: 8 CVAHRGFSGKAPENTLAAVRMAIALPFVRWMEIDVQLTKDGVPVVIHDFSLDRTTNGHGK 67 C G G + + R + V V T G+ Sbjct: 3 CGISDGLPGFSYADADGKFSGIDVDI-CRGVAAAVFGDDTKVKYTPLTAKERFTALQSGE 61 Query: 68 VKNMDYEHVRRLDAGSWKGRAFRGERVPSLKEVLELASGRLRLNIELKTSGDMYPGLEQA 127 V + + G AF G L L+ EL + Sbjct: 62 VDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCI-QAGTD 120 Query: 128 VIDLVNAHGMRDEVVLTSFDAGALQRIKELDPRFRTGLIYDSRSGDPAR-KLKELD--CS 184 V + + + T + R + +S A Sbjct: 121 TELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWI 180 Query: 185 FLSISFDRLNPGLAKLLAERGIRVMAWTVNKAKEMRRLSAMHSEIMICTNRPDI 238 L + G + + A ++S+ + + Sbjct: 181 VLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQ-NVDEKAANP 233 >BH3700 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 284 Score = 135 bits (341), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 77/229 (33%), Positives = 128/229 (55%), Gaps = 17/229 (7%) Query: 5 KNMCVAHRGFSGKAPENTLAAVRMAIALPFVRWMEIDVQLTKDGVPVVIHDFSLDRTTNG 64 K + +AHRG SG APENT+AA + + V ++E DV +TKDG V++HD ++DRTTNG Sbjct: 38 KMINIAHRGASGHAPENTMAAFQKGFDMK-VDYIEFDVHMTKDGELVLMHDLTVDRTTNG 96 Query: 65 HGKVKNMDYEHVRRLDAGSWKGRAFRGERVPSLKEVLELASGRLRLNIELKTSGDMYPGL 124 HG V ++ + +++ DAGSW F GE+VP+ +E L++ G++ L IELK S + YPG+ Sbjct: 97 HGYVSDLTLDEIKQFDAGSWFHEDFVGEKVPTFEETLDVFRGKVGLLIELK-SPERYPGM 155 Query: 125 EQAVIDLVNAHGM----RDEVVLTSFDAGALQRIKELDPRFRTGLIYDSRSGDPARKLKE 180 E+ V + + M +V++ SF+ ++++ KEL P G++ +G + + Sbjct: 156 EEKVAEALKERKMDRSSNHKVIIQSFNHESMKKSKELLPHIPHGVL----AGPSWANVTD 211 Query: 181 LDCSFLSISFDRLNPGLAKLLAER-------GIRVMAWTVNKAKEMRRL 222 S D NP + + E G+++ +T ++ RL Sbjct: 212 EQLEEFSTYADYFNPNMTIVTPELVSRIHAVGMKIFPYTSRTQEQANRL 260 >CAC0430 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 249 Score = 130 bits (328), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 75/240 (31%), Positives = 134/240 (55%), Gaps = 4/240 (1%) Query: 9 VAHRGFSGKAPENTLAAVRMAIALPFVRWMEIDVQLTKDGVPVVIHDFSLDRTTNGHGKV 68 +AHRGFSGK PENT+ A + AI +E D+ +TKDG+ VV HD +DRTTNG G + Sbjct: 8 IAHRGFSGKYPENTMVAFKKAIEEK-CDGIETDLNMTKDGILVVCHDEKIDRTTNGCGYI 66 Query: 69 KNMDYEHVRRLDAGSWKGRAFRGERVPSLKEVLELASGRLRLNIELKTSGDM-YPGLEQA 127 K+ Y+ +++ DAG G F+GE++ + E+L+ + L + + Y +E+ Sbjct: 67 KDYTYKELKQFDAGIGYGEEFKGEKILCIDELLDYIKDKNLLLNLELKNNLIEYVDIEKK 126 Query: 128 VIDLVNAHGMRDEVVLTSFDAGALQRIKELDPRFRTGLIYDSRSGDPARKLKELDCSFLS 187 V+D ++ + + D ++++SF+ ++ ++KE D + GL+Y + + ++L L Sbjct: 127 VVDKIHEYKLEDNIIVSSFNHYSMVKLKEYDSTMKIGLLYSANLYNVHEYGRKLGAFSLH 186 Query: 188 ISFDR-LNPGLAKLLAERGIRVMAWTVNKAKEMRRLSAMHSEIMICTNRPDIWGDTFLEA 246 + +N + + + + I + +TVN+ ++MR+L + E +I TN PD EA Sbjct: 187 PYYPAVMNKEVVENIKKVKININTYTVNEEEDMRKLIGLGIEGII-TNYPDRLNKVLKEA 245 >YPO3792 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 249 Score = 123 bits (308), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 84/238 (35%), Positives = 122/238 (51%), Gaps = 5/238 (2%) Query: 9 VAHRGFSGKAPENTLAAVRMAIALPFVRWMEIDVQLTKDGVPVVIHDFSLDRTTNGHGKV 68 VAHRG APENTLAA+ + + +E D +L++DG ++HD +L+RT+NG G Sbjct: 11 VAHRGGGSLAPENTLAAIDVGARYGH-KMIEFDAKLSQDGQIFLLHDDTLERTSNGWGVA 69 Query: 69 KNMDYEHVRRLDAGSWKGRAFRGERVPSLKEV-LELASGRLRLNIELK--TSGDMYPGLE 125 + +E + +LDAG W +AFRGER+P L EV A + NIE+K T D G Sbjct: 70 GELTWEKLIQLDAGDWFSKAFRGERLPLLSEVAARCAQHGMAANIEIKPTTGTDAPTGRA 129 Query: 126 QAVIDLVNAHGMRDEVVLTSFDAGALQRIKELDPRFRTGLIYDSRSGDPARKLKELDCSF 185 A+ G +L+SF AL + P GL+ D + A +LDC Sbjct: 130 IALAARALWQGQPIPPLLSSFSVDALAAAQLAVPELPRGLLLDKWDDNWAALTTQLDCVS 189 Query: 186 LSISFDRLNPGLAKLLAERGIRVMAWTVNKAKEMRRLSAMHSEIMICTNRPDIWGDTF 243 L I+ +L LL G+R++ +TVN+ + R L + ICT+R D+ G F Sbjct: 190 LHINHKQLTAERVALLKAAGLRILVYTVNQPERARELLNWGVDC-ICTDRIDLLGSDF 246 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,356,471 Number of extensions: 762147 Number of successful extensions: 1644 Number of sequences better than 1.0e-05: 57 Number of HSP's gapped: 1564 Number of HSP's successfully gapped: 57 Length of query: 246 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 142 Effective length of database: 26,383,695 Effective search space: 3746484690 Effective search space used: 3746484690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)