BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0037.p (432 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB1E7.07_4 [ER] COG0493 NADPH-dependent glutamate synthase be... 481 e-136 YDL171c_4 [ER] COG0493 NADPH-dependent glutamate synthase beta c... 457 e-128 VC2374 [ER] COG0493 NADPH-dependent glutamate synthase beta chai... 396 e-110 DR0182 [ER] COG0493 NADPH-dependent glutamate synthase beta chai... 387 e-107 CAC1674 [ER] COG0493 NADPH-dependent glutamate synthase beta cha... 377 e-104 >SPAPB1E7.07_4 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 481 bits (1239), Expect = e-136, Method: Compositional matrix adjust. Identities = 0/431 (0%), Positives = 0/431 (0%), Gaps = 2/431 (0%) Query: 4 GCPLHNLIPEWNDMVYRGNWEVALKRLHKTNNFPEFTGRVCPAPCEGACTVGMNGKPVTI 63 + P + N P + A + Sbjct: 57 RITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHL 116 Query: 64 KSIEKAIVDRGFEEGWIVPQPPLTRTGKKVAIVGSGPAGLACAAQLNKAGHSVTVYERAD 123 S+ + +P + G A ++ ++ Sbjct: 117 VSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEV 176 Query: 124 RIGGLLTYGIPNMKLDKKTVQRRVDLLAAEGITFVTRTEIGR--DIPASQLKEEHDAVVL 181 I Y L + ++ + + Sbjct: 177 DIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQ 236 Query: 182 CGGSTQARDLALEGRGLRGIHQAMEFLTLNTKSLLDSGLADGEYISAAXXXXXXXXXXXX 241 Sbjct: 237 296 Query: 242 XXXCVATSIRHGCRSVIQLEIMPQSPLTRQAGNPWPEWPKVLKVDYGQQEAASLYHEDPR 301 Sbjct: 297 356 Query: 302 RYLVNTKRFVGDDGGHVQELHTVRIEWSRNEQGRMVPVEVPGSEEVIKAQLVLLALGFTG 361 Sbjct: 357 416 Query: 362 PEETVLGQLGVERDERSNAKAEFGAQATNVEGVFAAGDIRRGQSLVVWAINEGRQAAREV 421 Sbjct: 417 476 Query: 422 DRFLTGSSNLP 432 Sbjct: 477 487 >YDL171c_4 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 457 bits (1176), Expect = e-128, Method: Compositional matrix adjust. Identities = 0/435 (0%), Positives = 0/435 (0%), Gaps = 9/435 (2%) Query: 3 SGCPLHNLIPEWNDMVYRGNWEVALKRLHKTNNFPEFTGRVCPAPCEGACTVGMNGKPVT 62 + + P + N P + A Sbjct: 56 ARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPH 115 Query: 63 IKSIEKAIVDRGFEEGWIVPQPPLTRTGKKVAIVGSGPAGLACAAQLNKAGHSVTVYERA 122 + S+ + +P + G A ++ ++ Sbjct: 116 LVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCE 175 Query: 123 DRIGGLLTYGIPNMKLDKKTVQRRVDLLAAEGITFVTRTEIGRDIPASQLKEEHDAVVLC 182 I Y L + ++ L+E++ Sbjct: 176 VDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENG 235 Query: 183 GGSTQARDLALEGRGLRGIHQAMEFLTLNTKSLLDSGLADGEYISAAXXXXXXXXX--XX 240 Sbjct: 236 --- 292 Query: 241 XXXXCVATSIRHGCRSVIQLEIMPQSPLTRQAGNPWPEWPKVLKVDYGQQEAASLYHEDP 300 Sbjct: 293 352 Query: 301 RRYLVNTKRFVGDDGGHVQELHTVRIEWSRNEQGRMVPVEVPGSEEVIKAQLVLLALGFT 360 Sbjct: 353 412 Query: 361 GPEETVLGQLGVERDERSNAKAEFGAQATNVEG--VFAAGDIRRGQSLVVWAINEGRQAA 418 Sbjct: 413 - 471 Query: 419 REVDRFLT-GSSNLP 432 Sbjct: 472 486 >VC2374 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 396 bits (1017), Expect = e-110, Method: Compositional matrix adjust. Identities = 0/432 (0%), Positives = 0/432 (0%), Gaps = 13/432 (3%) Query: 3 SGCPLHNLIPEWNDMVYRGNWEVALKRLHKTNNFPEFTGRVCPAPCEGACTVGMNGKPVT 62 + + + ++ + + + P Sbjct: 57 RITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHL 116 Query: 63 IKSIEKAIVDRGFEEGWIVPQPPLTRTGKKVAIVGSGPAGLACAAQLNKAGHSVTVYERA 122 + + V + + A Sbjct: 117 VSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEV 176 Query: 123 DRIGGLLTYGIPNMKLDKKTVQRRVDLLAAEGITFVTRTEIGRDIPASQLKEEHDAVVLC 182 D + +P+ + V + +T + + ++ Sbjct: 177 DIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQ 236 Query: 183 GGSTQARDLALEGRGLRGIHQAMEFLTLNTKSLLDSGLADGEYISAAXXXXXXXXXXXXX 242 Sbjct: 237 - 295 Query: 243 XXCVATSIRHGCRSVIQLEIMPQSPLTRQAGNPWPEWPKVLKVDYGQQEAASLYHEDPRR 302 Sbjct: 296 ------ 349 Query: 303 YLVNTKRFVGDDGGHVQELHTVRIEWSRNEQG-RMVPVEVPGSEEVIKAQLVLLALGFTG 361 Sbjct: 350 409 Query: 362 PE-ETVLGQLGVERDERSN-AKAEFGAQATNVEGVFAAGDIRRGQSLVVWAINEGRQAAR 419 Sbjct: 410 --- 466 Query: 420 EVDRFLTGSSNL 431 Sbjct: 467 478 >DR0182 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 387 bits (995), Expect = e-107, Method: Compositional matrix adjust. Identities = 0/447 (0%), Positives = 0/447 (0%), Gaps = 32/447 (7%) Query: 3 SGCPLHNLIPEWNDMVYRGNWEVALKRLHKTNNFPEFTGRVCPAPCEGACTVGMNGKPVT 62 + + P + N P + A Sbjct: 56 ARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPH 115 Query: 63 IKSIEKAIVDRGFEEGWIVPQPPLTRTGKKVAIVGSGPAGLACAAQLNKAGHSVTVYERA 122 + S+ + +P + G A ++ ++ Sbjct: 116 LVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCE 175 Query: 123 DRIGGLLTYGIPNMKLDKKTVQRRVDLLAAEGITFVTRTEIG--------------RDIP 168 I Y L + ++ ++ Sbjct: 176 VDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENG 235 Query: 169 ASQLKEEHDAVVLCGGSTQARDLALEGRGLRGIHQAMEFLTLNTKSLLDSGLADGEYISA 228 Sbjct: 236 Q--- 292 Query: 229 AXXXXXXXXXXXXXXXCVATSIRHGCRSVIQLEIMPQSPLTRQAGNPWPEWPKVLKVDYG 288 Sbjct: 293 352 Query: 289 QQEAASLYHEDPRRYLVNTKRFVGDDGGHVQELHTVRIEWSRNEQGRMVPVEVPGSEEVI 348 Sbjct: 353 ------------ 400 Query: 349 KAQLVLLALGFTGPEETVLGQLGVERDERSNAKA---EFGAQATNVEGVFAAGDIRRGQS 405 Sbjct: 401 460 Query: 406 LVVWAINEGRQAAREVDRFLTGSSNLP 432 Sbjct: 461 487 >CAC1674 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 377 bits (967), Expect = e-104, Method: Compositional matrix adjust. Identities = 0/431 (0%), Positives = 0/431 (0%), Gaps = 13/431 (3%) Query: 4 GCPLHNLIPEWNDMVYRGNWEVALKRLHKTNNFPEFTGRVCPAPCEGACTVGMNGKPVTI 63 + P + N P + A + Sbjct: 57 RITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHL 116 Query: 64 KSIEKAIVDRGFEEGWIVPQPPLTRTGKKVAIVGSGPAGLACAAQLNKAGHSVTVYERAD 123 S+ + +P + G A ++ ++ Sbjct: 117 VSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEV 176 Query: 124 RIGGLLTYGIPNMKLDKKTVQRRVDLLAAEGITFVTRTEIGRDIPASQLKEEHDAVVLCG 183 I Y L + ++ L+E++ Sbjct: 177 DIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQ 236 Query: 184 GSTQARDLALEGR-GLRGIHQAMEFLTLNTKSLLDSGLA-DGEYISAAXXXXXXXXXXXX 241 L + R Sbjct: 237 ILLAFPRLFIVARR-- 294 Query: 242 XXXCVATSIRHGCRSVIQLEIMPQSPLTRQAGNPWPEWPKVLKVDYGQQEAASLYHEDPR 301 Sbjct: 295 ------ 348 Query: 302 RYLVNTKRFVGDDGGHVQELHTVRIEWSRNEQGRMVPVEVPGSEEVIKAQLVLLALGFTG 361 Sbjct: 349 - 407 Query: 362 PEET-VLGQLGVERDERSNAKAEFGAQATNVEGVFAAGDIRRGQSLVVWAINEGRQAARE 420 Sbjct: 408 - 466 Query: 421 VDRFLTGSSNL 431 Sbjct: 467 477 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,575,096 Number of extensions: 1303190 Number of successful extensions: 4496 Number of sequences better than 1.0e-05: 62 Number of HSP's gapped: 4384 Number of HSP's successfully gapped: 77 Length of query: 432 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 323 Effective length of database: 25,807,025 Effective search space: 8335669075 Effective search space used: 8335669075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)