BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0459.0035.p
         (180 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CAC2749 [R] COG0622 Predicted phosphoesterase                         200   1e-51
TM1101 [R] COG0622 Predicted phosphoesterase                          110   2e-24
STM1101 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e...   110   2e-24
CAC2664 [R] COG0622 Predicted phosphoesterase                          55   5e-08

>CAC2749 [R] COG0622 Predicted phosphoesterase
          Length = 180

 Score =  200 bits (508), Expect = 1e-51,   Method: Compositional matrix adjust.
 Identities = 90/180 (50%), Positives = 128/180 (71%)

Query: 1   MKLMFISDIHGSLYWXXXXXXXXXXXXPHTLVVLGDFLYHGPRNPLPDGYDPQGVAARLN 60
           MK+ F+SDIHGSL++               +V+LGD LYHG RNPLP GY+P+ V+  LN
Sbjct: 1   MKIFFMSDIHGSLHYLEKALARFKEENADFIVILGDELYHGARNPLPLGYNPKAVSELLN 60

Query: 61  AYDKSLVAVRGNCDAEVDQMLLQFPMMGDYVLILHEGRKIYATHGHGFSIDNLPPLAPGD 120
           +Y + ++A+RGNCD+EVD+M+L +P+M  Y  IL++ ++++ THGH +  +NLP L  GD
Sbjct: 61  SYSEQIIAIRGNCDSEVDEMVLTYPIMSTYSTILYKDKRLFLTHGHVYGEENLPRLRRGD 120

Query: 121 VFIQGHTHLPVADVKEGITVLNPGSISLPKENNPNSYAILENGEFIIKDFAGKIVKQIRL 180
           VF+ GHTH+PVA  K+ I ++NPGSIS+PKEN+PNSYA+ EN  F IKD AG    ++ +
Sbjct: 121 VFLYGHTHVPVAKKKDDIYIINPGSISIPKENSPNSYAVFENDLFEIKDLAGNTFMKLNI 180


>TM1101 [R] COG0622 Predicted phosphoesterase
          Length = 189

 Score =  110 bits (274), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 66/187 (35%), Positives = 101/187 (54%), Gaps = 11/187 (5%)

Query: 1   MKLMFISDIHGSLYWXXXXXXXXXXXXPHTLVVLGDFLYHGPRNPLPDGYDPQGVAARLN 60
           ++++ ISD HGSL                 +  LGD LYHGPRNPLP+GY P+ +A+ L 
Sbjct: 3   LRILVISDTHGSLSCTEKALKSAGNFD--EIWHLGDVLYHGPRNPLPEGYSPKELASLLK 60

Query: 61  AYDKSLVAVRGNCDAEVDQMLLQFPMMGDYVLILHEGRKIYATHGHGFSIDNLPPLAPG- 119
            +   +  +RGNCDA+VD  +L+ P M    +      KI   HG  F  +   P++   
Sbjct: 61  KH--RVKYIRGNCDADVDIRVLEIPEMPRIGMEFLGDVKILLVHGDQFEYEGGDPVSLAQ 118

Query: 120 ----DVFIQGHTHLPVADVKEGITVLNPGSISLPKENNPNSYAILENGE--FIIKDFAGK 173
                V + GHTH+P+A+  EG+ +LNPGS+SLPK     ++ I++  E  F +    G 
Sbjct: 119 AHGCSVVLFGHTHVPLAERCEGVLLLNPGSVSLPKSEAGPTFGIIDMDEKKFYLCSLEGD 178

Query: 174 IVKQIRL 180
           ++K++ L
Sbjct: 179 VLKEVML 185


>STM1101 [Q] COG0179 2-keto-4-pentenoate
           hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase
           (catechol pathway)
          Length = 189

 Score =  110 bits (274), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 66/187 (35%), Positives = 101/187 (54%), Gaps = 11/187 (5%)

Query: 1   MKLMFISDIHGSLYWXXXXXXXXXXXXPHTLVVLGDFLYHGPRNPLPDGYDPQGVAARLN 60
           ++++ ISD HGSL                 +  LGD LYHGPRNPLP+GY P+ +A+ L 
Sbjct: 3   LRILVISDTHGSLSCTEKALKSAGNFD--EIWHLGDVLYHGPRNPLPEGYSPKELASLLK 60

Query: 61  AYDKSLVAVRGNCDAEVDQMLLQFPMMGDYVLILHEGRKIYATHGHGFSIDNLPPLAPG- 119
            +   +  +RGNCDA+VD  +L+ P M    +      KI   HG  F  +   P++   
Sbjct: 61  KH--RVKYIRGNCDADVDIRVLEIPEMPRIGMEFLGDVKILLVHGDQFEYEGGDPVSLAQ 118

Query: 120 ----DVFIQGHTHLPVADVKEGITVLNPGSISLPKENNPNSYAILENGE--FIIKDFAGK 173
                V + GHTH+P+A+  EG+ +LNPGS+SLPK     ++ I++  E  F +    G 
Sbjct: 119 AHGCSVVLFGHTHVPLAERCEGVLLLNPGSVSLPKSEAGPTFGIIDMDEKKFYLCSLEGD 178

Query: 174 IVKQIRL 180
           ++K++ L
Sbjct: 179 VLKEVML 185


>CAC2664 [R] COG0622 Predicted phosphoesterase
          Length = 155

 Score = 55.5 bits (132), Expect = 5e-08,   Method: Compositional matrix adjust.
 Identities = 39/120 (32%), Positives = 59/120 (49%), Gaps = 17/120 (14%)

Query: 49  GYDPQGVAARLNAYDKSLVAVRGNCDAEVDQMLLQFPMMGDYVLILHEGRKIYATHGH-- 106
           G + Q +      Y+  L+ +RGNCD E      + P    ++L    G+K + THG   
Sbjct: 33  GDNVQDIDEIKKYYNGELIYIRGNCDDE------KIPSEKTFLL---GGKKFFITHGDRY 83

Query: 107 --GFSIDNLPPLAP---GDVFIQGHTHLPVADVKEGITVLNPGSISLPKENNPNSYAILE 161
              +S+  L   A     D+ + GHTH+   D  +GI  +NPGS+S P+ N  NS A +E
Sbjct: 84  GVKYSMMKLEYRAKELEADIVLFGHTHISQIDFNDGIWYINPGSVSYPR-NASNSTAYIE 142


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.322    0.143    0.442 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 14,070,910
Number of extensions: 562253
Number of successful extensions: 1161
Number of sequences better than 1.0e-05: 5
Number of HSP's gapped: 1156
Number of HSP's successfully gapped: 5
Length of query: 180
Length of database: 38,378,431
Length adjustment: 100
Effective length of query: 80
Effective length of database: 26,845,031
Effective search space: 2147602480
Effective search space used: 2147602480
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 112 (47.8 bits)