BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0033.p (181 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0293 [R] COG0388 Predicted amidohydrolase 235 2e-62 YPO0938 [R] COG0388 Predicted amidohydrolase 208 4e-54 DR2535 [R] COG0388 Predicted amidohydrolase 199 2e-51 CC0212 [R] COG0388 Predicted amidohydrolase 182 2e-46 Cj0947c [R] COG0388 Predicted amidohydrolase 142 4e-34 >PA0293 [R] COG0388 Predicted amidohydrolase Length = 292 Score = 235 bits (600), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 107/165 (64%), Positives = 125/165 (75%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 M+CS D NI +AE LVR FETPYFCQK DY AT +E N A Sbjct: 12 MACSWDRPANIARAEKLVRQAAARGAQIILIQELFETPYFCQKPNPDYLQLATTVEENAA 71 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 + HFQA+A+ELQVVLPISF+E+ AR+NS+AVIDADG LG YRKSHIPDGPGY EK+Y Sbjct: 72 IAHFQALARELQVVLPISFFERAGRARFNSIAVIDADGGNLGVYRKSHIPDGPGYHEKYY 131 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVW TRYA+IGVG+CWDQW+PE+AR M+L+GAE+LFY Sbjct: 132 FNPGDTGFKVWQTRYARIGVGICWDQWFPESARSMALLGAELLFY 176 >YPO0938 [R] COG0388 Predicted amidohydrolase Length = 294 Score = 208 bits (529), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 91/165 (55%), Positives = 119/165 (72%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 M+CS D+ +NI AE LVR F PYFC + ++YA A EL+++ Sbjct: 11 MACSWDLPKNIENAEKLVRQAHAKGAQIILIQELFAAPYFCIDQSPEHYALAQELDNSPL 70 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 + HF +A EL+VVLP+SF+EK N A YNSL +IDADG+VL YRK+HIP+GP Y+EK + Sbjct: 71 IKHFSKLAAELEVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIPNGPAYQEKQF 130 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 F PGDTGFKVW TRYAK+GVG+CWDQW+PE AR ++L+GAE++FY Sbjct: 131 FIPGDTGFKVWQTRYAKVGVGICWDQWFPETARSLALLGAEVIFY 175 >DR2535 [R] COG0388 Predicted amidohydrolase Length = 297 Score = 199 bits (505), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 88/165 (53%), Positives = 112/165 (67%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 M + +++N+ +A VR FE YFCQ E+ DY+ A LE + Sbjct: 15 MHMTDQLEDNVERAAEHVREAARRGAQVILLPELFENLYFCQVEREDYFGLAHPLEGHPF 74 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 + FQ +A+EL VVLP+S++EK A YNSL IDA G +LG YRK+HIPDGPGYEEK+Y Sbjct: 75 IGRFQELARELNVVLPVSYFEKAGQAHYNSLVCIDAGGELLGNYRKTHIPDGPGYEEKYY 134 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVW+TR+ ++GVG+CWDQWYPE ARVM L GA+ L Y Sbjct: 135 FNPGDTGFKVWDTRFGRVGVGICWDQWYPETARVMMLQGADFLLY 179 >CC0212 [R] COG0388 Predicted amidohydrolase Length = 292 Score = 182 bits (463), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 81/160 (50%), Positives = 106/160 (66%) Query: 6 DIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKAVNHFQ 65 D+ NI+K E +R F+ PYFC ++ ++A A + V Sbjct: 17 DLQANIKKTEGFIREAASKGAQVILPSELFQGPYFCVAQEERWFAQAHPWREHPVVKAIA 76 Query: 66 AIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFYFNPGD 125 +A EL VV+PIS +E++ +NSL + DADG+++G YRKSHIPDGPGY EK+YF PGD Sbjct: 77 PLAGELGVVIPISIFEREGPHYFNSLVMADADGSLMGVYRKSHIPDGPGYMEKYYFRPGD 136 Query: 126 TGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 TGFKVW+TR+ +IGVG+CWDQWYPE AR M+LMGAE LFY Sbjct: 137 TGFKVWDTRFGRIGVGICWDQWYPECARAMALMGAEALFY 176 >Cj0947c [R] COG0388 Predicted amidohydrolase Length = 290 Score = 142 bits (357), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 68/131 (51%), Positives = 92/131 (70%), Gaps = 3/131 (2%) Query: 36 ETPYFCQKEKSDYYAYATELEHNKAVNHFQAIAKELQVVLPISFYEKKNYARYNSLAVI- 94 ++ YFCQ E D++ YA + E K V + IA++ Q+VL S +EK++ Y++ AV+ Sbjct: 43 QSEYFCQSENVDFFDYANDYE--KDVKFWANIARKNQIVLIASLFEKRSAGLYHNTAVVF 100 Query: 95 DADGTVLGKYRKSHIPDGPGYEEKFYFNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARV 154 + DG++ GKYRK HIPD P + EKFYF PGD GF+ NT K+GV +CWDQWYPEAAR+ Sbjct: 101 EKDGSIAGKYRKMHIPDDPCFYEKFYFTPGDLGFEPINTSLGKLGVLICWDQWYPEAARI 160 Query: 155 MSLMGAEILFY 165 M+L GAEIL Y Sbjct: 161 MALKGAEILIY 171 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,319,160 Number of extensions: 539022 Number of successful extensions: 755 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 750 Number of HSP's successfully gapped: 31 Length of query: 181 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 81 Effective length of database: 26,845,031 Effective search space: 2174447511 Effective search space used: 2174447511 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 112 (47.8 bits)