BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0013.p (82 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr7009 [L] COG1943 Transposase and inactivated derivatives 62 2e-10 VNG0043H [L] COG1943 Transposase and inactivated derivatives 62 2e-10 HP1008 [L] COG1943 Transposase and inactivated derivatives 56 1e-08 HP0414 [L] COG1943 Transposase and inactivated derivatives 56 1e-08 DR1652 [L] COG1943 Transposase and inactivated derivatives 55 2e-08 >alr7009 [L] COG1943 Transposase and inactivated derivatives Length = 359 Score = 62.4 bits (150), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 2/83 (2%), Positives = 9/83 (10%), Gaps = 1/83 (1%) Query: 1 VIFESIQMNYNITLQEWNHDKDHVHILMKAHPNSDISKFLNAYKSASSRLIKKEFPI-IR 59 + L + + + I + Sbjct: 40 HGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAV 99 Query: 60 QQLWKEYFWSRSYCLLTTGGGPI 82 + + Sbjct: 100 HNEEQLAALEEASLDEPVTVWMK 122 >VNG0043H [L] COG1943 Transposase and inactivated derivatives Length = 130 Score = 62.4 bits (150), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 29/77 (37%), Positives = 45/77 (58%), Gaps = 1/77 (1%) Query: 2 IFESIQMNYNITLQEWNHDKDHVHILMKAHPNSDISKFLNAYKSASSRLIKKEFPIIRQQ 61 I I ++ ++ + DHVHIL + P +D++KF+N+ K +SR I+ EFP + Q Sbjct: 41 IAHDIADDFEADIKNVDGGTDHVHILFQTKPTTDLTKFINSLKGVTSRRIRSEFPEVTQT 100 Query: 62 LWKEYFWSRSYCLLTTG 78 L ++ FW Y L TTG Sbjct: 101 L-EDAFWQPGYFLATTG 116 >HP1008 [L] COG1943 Transposase and inactivated derivatives Length = 138 Score = 56.2 bits (134), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 26/82 (31%), Positives = 45/82 (54%), Gaps = 1/82 (1%) Query: 2 IFESIQMNYNITLQEWNHDKDHVHILMKAHPNSDISKFLNAYKSASSRLIKK-EFPIIRQ 60 +F + ++ L E++ + DHVH+L+ P +SK +N+ K SSRL ++ F + Sbjct: 43 VFAKVCKDFESELVEFDGESDHVHLLINYPPKVSVSKLVNSLKGVSSRLTRQHHFKSVEA 102 Query: 61 QLWKEYFWSRSYCLLTTGGGPI 82 LW ++ WS SY + G P+ Sbjct: 103 SLWGKHLWSPSYFAGSCGDAPL 124 >HP0414 [L] COG1943 Transposase and inactivated derivatives Length = 138 Score = 56.2 bits (134), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 26/82 (31%), Positives = 45/82 (54%), Gaps = 1/82 (1%) Query: 2 IFESIQMNYNITLQEWNHDKDHVHILMKAHPNSDISKFLNAYKSASSRLIKK-EFPIIRQ 60 +F + ++ L E++ + DHVH+L+ P +SK +N+ K SSRL ++ F + Sbjct: 43 VFAKVCKDFESELVEFDGESDHVHLLINYPPKVSVSKLVNSLKGVSSRLTRQHHFKSVEA 102 Query: 61 QLWKEYFWSRSYCLLTTGGGPI 82 LW ++ WS SY + G P+ Sbjct: 103 SLWGKHLWSPSYFAGSCGDAPL 124 >DR1652 [L] COG1943 Transposase and inactivated derivatives Length = 140 Score = 55.5 bits (132), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 23/56 (41%), Positives = 35/56 (62%) Query: 22 DHVHILMKAHPNSDISKFLNAYKSASSRLIKKEFPIIRQQLWKEYFWSRSYCLLTT 77 DHVH+L+ A P I F+ A K AS+R + +P ++++LW W+ SYC+LT Sbjct: 66 DHVHLLLSATPQQAIPDFVKALKGASARRMFVAYPQLKEKLWGGNLWNPSYCILTV 121 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.135 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 5,803,042 Number of extensions: 175057 Number of successful extensions: 589 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 589 Number of HSP's successfully gapped: 20 Length of query: 82 Length of database: 38,378,431 Length adjustment: 52 Effective length of query: 30 Effective length of database: 32,381,063 Effective search space: 971431890 Effective search space used: 971431890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 109 (46.6 bits)