BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0011.p (218 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value FN1856 [I] COG2057 Acyl CoA:acetate/3-ketoacid CoA transferase, ... 247 1e-65 Rv2503c [I] COG2057 Acyl CoA:acetate/3-ketoacid CoA transferase,... 231 6e-61 MT2578 [I] COG2057 Acyl CoA:acetate/3-ketoacid CoA transferase, ... 231 6e-61 BS_yxjE [I] COG2057 Acyl CoA:acetate/3-ketoacid CoA transferase,... 229 3e-60 PA2000 [I] COG2057 Acyl CoA:acetate/3-ketoacid CoA transferase, ... 222 4e-58 >FN1856 [I] COG2057 Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit Length = 217 Score = 247 bits (630), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 126/211 (59%), Positives = 161/211 (76%), Gaps = 3/211 (1%) Query: 4 REFIVRRIAQEFKDGDVVNLGIGLPTMAVDYIPDNVHIMLQAENGILGVGPKAAKEQENI 63 RE I +R+AQEF DG VVNLGIGLPT+ +Y+ D + ++ Q+ENG +GVGP K +E+ Sbjct: 9 REVIAKRVAQEFHDGYVVNLGIGLPTLVANYVGD-MDVIFQSENGCIGVGPAPEKGKEDP 67 Query: 64 DCIDSGGSYVTTMSGACYFDSALSFSIIRGGHVDITVLGALEVDKNGNLASWMIPGKKVP 123 +++G ++T GA +FDSA SF IIRGGHVD TVLGALEVD+ GNLA+WMIPGKKVP Sbjct: 68 YLVNAGAGFITAAKGAMFFDSAYSFGIIRGGHVDATVLGALEVDEKGNLANWMIPGKKVP 127 Query: 124 GMGGAMDLVVGAKRVIVAMDHVNKNGEPKILEKCKLPLTAVNVVKTIVTEMAVIDVIPGT 183 GMGGAMDLVVGAK+VIVAM+H + NG KIL++CKLPLTAV VV I+TE AV +V Sbjct: 128 GMGGAMDLVVGAKKVIVAMEHTS-NGAIKILKECKLPLTAVGVVDLIITEKAVFEVTD-K 185 Query: 184 GLMLKEIAPGLSVEDVQKATGAKLLVSPNLK 214 GL+LKEI P S+ED++ T A +++ +LK Sbjct: 186 GLVLKEITPYSSLEDIKATTAADFIIADDLK 216 >Rv2503c [I] COG2057 Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit Length = 218 Score = 231 bits (589), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 115/207 (55%), Positives = 152/207 (73%), Gaps = 1/207 (0%) Query: 3 NREFIVRRIAQEFKDGDVVNLGIGLPTMAVDYIPDNVHIMLQAENGILGVGPKAAKEQEN 62 +R+ + R+A EF+DG VNLGIG+PT+ ++IPD VH++L +ENGILGVGP +E + Sbjct: 7 SRDEMAARVAAEFEDGQYVNLGIGMPTLIPNHIPDGVHVVLHSENGILGVGPYPRREDVD 66 Query: 63 IDCIDSGGSYVTTMSGACYFDSALSFSIIRGGHVDITVLGALEVDKNGNLASWMIPGKKV 122 D I++G VTT+ GA +F S+ SF IIRGGH+D+ VLGA++V G+LA+WMIPGK V Sbjct: 67 ADLINAGKETVTTLPGAAFFSSSTSFGIIRGGHLDVAVLGAMQVSVTGDLANWMIPGKMV 126 Query: 123 PGMGGAMDLVVGAKRVIVAMDHVNKNGEPKILEKCKLPLTAVNVVKTIVTEMAVIDVIPG 182 GMGGAMDLV GA++VIV M+H K+G PKILE+C LPLT V V IVTE+AVIDV Sbjct: 127 KGMGGAMDLVHGARKVIVMMEHTAKDGSPKILERCTLPLTGVGCVDRIVTELAVIDVC-A 185 Query: 183 TGLMLKEIAPGLSVEDVQKATGAKLLV 209 GL L + APG+SV++V T L++ Sbjct: 186 DGLHLVQTAPGVSVDEVVAKTQPPLVL 212 >MT2578 [I] COG2057 Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit Length = 218 Score = 231 bits (589), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 115/207 (55%), Positives = 152/207 (73%), Gaps = 1/207 (0%) Query: 3 NREFIVRRIAQEFKDGDVVNLGIGLPTMAVDYIPDNVHIMLQAENGILGVGPKAAKEQEN 62 +R+ + R+A EF+DG VNLGIG+PT+ ++IPD VH++L +ENGILGVGP +E + Sbjct: 7 SRDEMAARVAAEFEDGQYVNLGIGMPTLIPNHIPDGVHVVLHSENGILGVGPYPRREDVD 66 Query: 63 IDCIDSGGSYVTTMSGACYFDSALSFSIIRGGHVDITVLGALEVDKNGNLASWMIPGKKV 122 D I++G VTT+ GA +F S+ SF IIRGGH+D+ VLGA++V G+LA+WMIPGK V Sbjct: 67 ADLINAGKETVTTLPGAAFFSSSTSFGIIRGGHLDVAVLGAMQVSVTGDLANWMIPGKMV 126 Query: 123 PGMGGAMDLVVGAKRVIVAMDHVNKNGEPKILEKCKLPLTAVNVVKTIVTEMAVIDVIPG 182 GMGGAMDLV GA++VIV M+H K+G PKILE+C LPLT V V IVTE+AVIDV Sbjct: 127 KGMGGAMDLVHGARKVIVMMEHTAKDGSPKILERCTLPLTGVGCVDRIVTELAVIDVC-A 185 Query: 183 TGLMLKEIAPGLSVEDVQKATGAKLLV 209 GL L + APG+SV++V T L++ Sbjct: 186 DGLHLVQTAPGVSVDEVVAKTQPPLVL 212 >BS_yxjE [I] COG2057 Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit Length = 216 Score = 229 bits (583), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 108/210 (51%), Positives = 154/210 (73%), Gaps = 1/210 (0%) Query: 4 REFIVRRIAQEFKDGDVVNLGIGLPTMAVDYIPDNVHIMLQAENGILGVGPKAAKEQENI 63 R+ +V+R QE KDG VNLGIG+PT+ + IPD VH+MLQ+ENG+LG+GP + E+ Sbjct: 5 RKRMVKRAVQEIKDGMNVNLGIGMPTLVANEIPDGVHVMLQSENGLLGIGPYPLEGTEDA 64 Query: 64 DCIDSGGSYVTTMSGACYFDSALSFSIIRGGHVDITVLGALEVDKNGNLASWMIPGKKVP 123 D I++G +T ++GA YFDSA SF++IRGGH+D+ +LG +EV + G+LA+WMIPGK V Sbjct: 65 DLINAGKETITEVTGASYFDSAESFAMIRGGHIDLAILGGMEVSEQGDLANWMIPGKMVK 124 Query: 124 GMGGAMDLVVGAKRVIVAMDHVNKNGEPKILEKCKLPLTAVNVVKTIVTEMAVIDVIPGT 183 GMGGAMDLV GAKR++V M+HVNK+GE K+ + C LPLT VV ++T++AV D + G Sbjct: 125 GMGGAMDLVNGAKRIVVIMEHVNKHGESKVKKTCSLPLTGQKVVHRLITDLAVFDFVNGR 184 Query: 184 GLMLKEIAPGLSVEDVQKATGAKLLVSPNL 213 + L E+ G+++E+V + T A VS ++ Sbjct: 185 -MTLTELQDGVTIEEVYEKTEADFAVSQSV 213 >PA2000 [I] COG2057 Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit Length = 218 Score = 222 bits (565), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 106/207 (51%), Positives = 150/207 (72%), Gaps = 1/207 (0%) Query: 3 NREFIVRRIAQEFKDGDVVNLGIGLPTMAVDYIPDNVHIMLQAENGILGVGPKAAKEQEN 62 RE + +R+A+E KDG VNLGIG+PT+ +Y+P+ + +MLQ+ENG+LG+G ++ + + Sbjct: 4 TREQMAQRVARELKDGYYVNLGIGIPTLVANYVPEGIDVMLQSENGLLGMGAFPSEAEVD 63 Query: 63 IDCIDSGGSYVTTMSGACYFDSALSFSIIRGGHVDITVLGALEVDKNGNLASWMIPGKKV 122 D I++G VT GA FDSA SF++IRGGHVD+TVLGA EVD GN+ASWMIPGK V Sbjct: 64 ADMINAGKQTVTARVGASIFDSAQSFAMIRGGHVDLTVLGAFEVDVRGNIASWMIPGKLV 123 Query: 123 PGMGGAMDLVVGAKRVIVAMDHVNKNGEPKILEKCKLPLTAVNVVKTIVTEMAVIDVIPG 182 GMGGAMDLV GA+ +IV M H +K+GE K+L C LPLT ++ ++T++A +++ G Sbjct: 124 KGMGGAMDLVAGAENIIVTMTHASKDGESKLLPYCTLPLTGCGCIRKVLTDLAYLEIEDG 183 Query: 183 TGLMLKEIAPGLSVEDVQKATGAKLLV 209 +L+E APG+SVE++ T KL+V Sbjct: 184 A-FILRERAPGVSVEEIVAKTAGKLIV 209 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,907,229 Number of extensions: 760739 Number of successful extensions: 6495 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 6476 Number of HSP's successfully gapped: 20 Length of query: 218 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 116 Effective length of database: 26,614,363 Effective search space: 3087266108 Effective search space used: 3087266108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)