BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0454.0017.p (280 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0042 [G] COG2211 Na+/melibiose symporter and related transpor... 182 4e-46 CAC3422 [G] COG2211 Na+/melibiose symporter and related transpor... 174 1e-43 BS_ydjD [G] COG2211 Na+/melibiose symporter and related transpor... 145 5e-35 yagG [G] COG2211 Na+/melibiose symporter and related transporters 142 4e-34 BS_ynaJ [G] COG2211 Na+/melibiose symporter and related transpor... 142 7e-34 >STM0042 [G] COG2211 Na+/melibiose symporter and related transporters Length = 457 Score = 182 bits (463), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 94/266 (35%), Positives = 144/266 (54%), Gaps = 1/266 (0%) Query: 4 REESNQKLSLLEKVSYGLGDTASNLIYAVVTTYLTFYFTDVYXXXXXXXXXXXXXXXXXD 63 + + LSLL+++SYG D A NL+Y +TY+ +++TDV D Sbjct: 9 QPSGSASLSLLQRISYGSLDVAGNLLYCFSSTYILYFYTDVAGISLAVAGVILLLARIID 68 Query: 64 MVDSPIFGILIDKTHTKWGKSRPWILWSCFPFTIVTILLFMGPELSASGKLVYAYIFYIA 123 +D+PI+GI+IDKT +++GK RPW LW PF + + L F P++S +GK +YA I Y+ Sbjct: 69 GIDAPIWGIIIDKTRSRYGKCRPWFLWLPLPFAVFSALSFWSPDISMTGKAIYAAISYMI 128 Query: 124 SNILYTAVNNXXXXXXXXXXXEIQERTVANSFRMFXXXXXXXXXXXXXXXXVAFFGNGDQ 183 ++IL+T +N +ER V NS+RM VAF GNGD Sbjct: 129 ASILFTGLNTPLSAILPLMTLSPKERLVLNSWRMTGGQIGVLLMNATALPLVAFLGNGDD 188 Query: 184 QKGFFHTMILFGAVGLVMFLITFFNTRERVQDR-SGGKSLPVRESVKAIKGNMPWVAGVL 242 GF +T I F + + L F N RE D+ LP+++S A+KGN PW+ VL Sbjct: 189 HAGFIYTAITFAIISCALTLFAFKNIREMDTDKIQHEPKLPMKKSFAAMKGNWPWILMVL 248 Query: 243 IGVVFFILYVVKLSSGIYYITYNLNQ 268 ++F+I + ++ +YY+TYNL++ Sbjct: 249 ANLIFWIALQQRNTTIVYYLTYNLDR 274 >CAC3422 [G] COG2211 Na+/melibiose symporter and related transporters Length = 445 Score = 174 bits (441), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 98/259 (37%), Positives = 150/259 (57%), Gaps = 2/259 (0%) Query: 10 KLSLLEKVSYGLGDTASNLIYAVVTTYLTFYFTDVYXXXXXXXXXXXXXXXXXDMVDSPI 69 K+ L+EK+SYGLGDTAS L + +V+ Y+ F++TDV+ D + PI Sbjct: 5 KIPLVEKISYGLGDTASILTFQMVSVYIMFFYTDVFGLSTASIAVLLLSARIIDALIDPI 64 Query: 70 FGILIDKTHTKWGKSRPWILWSCFPFTIVTILLFMGPELSASGKLVYAYIFYIASNILYT 129 GI+IDKT TKWGK RP+ LW P+ ++ I F P LS +GKL+YAYI YI+ NI+Y+ Sbjct: 65 IGIIIDKTSTKWGKCRPYFLWFSVPYGLIAIATFFTPHLSTNGKLIYAYITYISLNIVYS 124 Query: 130 AVNNXXXXXXXXXXXEIQERTVANSFRMFXXXXXXXXXXXXXXXXVAFFGNGDQQKGFFH 189 +N + +ERT + R+F V+ G G++QKGFF Sbjct: 125 IINLPITAILPSLTDDYKERTQTTAIRIFLASCGSIIVSTFTLPLVSILGEGNKQKGFFL 184 Query: 190 TMILFGAVGLVMFLITFFNTRERVQDRSGGKSLPVRESVKAIKGNMPWVAGVLIGVVFFI 249 TM+LFG + ++F+ TF T ER+ G +P+++S+ +K N+PW+ L+ V +I Sbjct: 185 TMVLFGFIASLLFVNTFIQTHERILANK-GNPIPLKKSIMTLK-NLPWLLITLVNVFTYI 242 Query: 250 LYVVKLSSGIYYITYNLNQ 268 +K SS +YY++YN+++ Sbjct: 243 GLNIKNSSMVYYLSYNISR 261 >BS_ydjD [G] COG2211 Na+/melibiose symporter and related transporters Length = 463 Score = 145 bits (367), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 83/265 (31%), Positives = 133/265 (50%), Gaps = 1/265 (0%) Query: 4 REESNQKLSLLEKVSYGLGDTASNLIYAVVTTYLTFYFTDVYXXXXXXXXXXXXXXXXXD 63 + +S+ LS E++ YG GD + N+I+ V YL FY+TDV D Sbjct: 15 QSKSSTGLSWFERIGYGFGDMSYNIIFQFVNAYLLFYYTDVGGIQPAVIATLFLVVRVLD 74 Query: 64 MVDSPIFGILIDKTHTKWGKSRPWILWSCFPFTIVTILLFMGPELSASGKLVYAYIFYIA 123 + PI G+++DKT+T+WGK+RP++LW FPF + T L F P +G +VYAY+ YI Sbjct: 75 AIFDPIMGLILDKTNTRWGKARPYLLWVAFPFALFTFLCFTTPHFGETGNMVYAYVTYIL 134 Query: 124 SNILYTAVNNXXXXXXXXXXXEIQERTVANSFRMFXXXXXXXXXXXXXXXXVAFFGNGDQ 183 + ++ ++ERTV + RM G DQ Sbjct: 135 LGMSFSMQTIPVNSLTGRMTNSVEERTVLTTTRMILVYIGILLSISCATPLATAIGGEDQ 194 Query: 184 QKGFFHTMILFGAVGLVMFLITFFNTRERVQDRSGGKSLPVRESVKAIKGNMPWVAGVLI 243 GF T +++ AV +V+ L +FF RER+Q + K +++++ + N P + + Sbjct: 195 AFGFQMTALIYAAVSIVLNLFSFFTVRERIQPKKRKKQG-IKKTLSVLFKNKPLLMLISS 253 Query: 244 GVVFFILYVVKLSSGIYYITYNLNQ 268 + F I + +KLS+ +YY TYN+N Sbjct: 254 FLAFAIGFNIKLSTMVYYFTYNVNH 278 >yagG [G] COG2211 Na+/melibiose symporter and related transporters Length = 460 Score = 142 bits (359), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 78/261 (29%), Positives = 128/261 (49%), Gaps = 3/261 (1%) Query: 10 KLSLLEKVSYGLGDTASNLIYAVVTTYLTFYFTDVYXXXXXXXXXXXXXXXXXDMVDSPI 69 +L++ +K+ YGLGDTA ++ L +++TDV+ D V P+ Sbjct: 3 QLTMKDKIGYGLGDTACGFVWQATMFLLAYFYTDVFGLSAGIMGTLFLVSRVLDAVTDPL 62 Query: 70 FGILIDKTHTKWGKSRPWILWSCFPFTIVTILLFMGPELSASGKLVYAYIFYIASNILYT 129 G+L+D+T T+ G+ RP++LW PF IV +L F P+ SA GK++YA + YI ++YT Sbjct: 63 MGLLVDRTRTRHGQFRPFLLWGAIPFGIVCVLTFYTPDFSAQGKIIYACVTYILLTLVYT 122 Query: 130 AVNNXXXXXXXXXXXEIQERTVANSFRMFXXXXXXXXXXXXXXXXVAFFGNGDQQKGFFH 189 VN + +ER S+R F V+ G GD+Q G+F Sbjct: 123 FVNVPYCAMPGVITADPKERHALQSWRFFLAAAGSLAISGIALPLVSIIGKGDEQVGYFG 182 Query: 190 TMILFGAVGLVMFLITFFNTRERVQ-DRSGGKSLPVRESVKAIKGNMPWVAGVLIGVVFF 248 M + G G+V+ + FF T+ER + G S V + +K + GN W ++ Sbjct: 183 AMCVLGLSGVVLLYVCFFTTKERYTFEVQPGSS--VAKDLKLLLGNSQWRIMCAFKMMAT 240 Query: 249 ILYVVKLSSGIYYITYNLNQP 269 VV+ + +Y++ Y ++ P Sbjct: 241 CSNVVRGGATLYFVKYVMDHP 261 >BS_ynaJ [G] COG2211 Na+/melibiose symporter and related transporters Length = 85 Score = 142 bits (357), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 0/266 (0%), Positives = 0/266 (0%), Gaps = 2/266 (0%) Query: 5 EESNQKLSLLEKVSYGLGDTASNLIYAVVTTYLTFYFTDVYXXXXXXXXXXXXXXXXXDM 64 + + + L T + Sbjct: 3 MAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIPLSEWTTSMYVIQSSMIFV 62 Query: 65 VDSPIFGILIDKTHTKWGKSRPWILWSCFPFTIVTILLFMGPELSASGKLVYAYIFYIAS 124 Sbjct: 63 122 Query: 125 NILYTAVNNXXXXXXXXXXXEIQERTVANSFRMFXXXXXXXXXXXXXXXXVAFFGN--GD 182 Sbjct: 123 182 Query: 183 QQKGFFHTMILFGAVGLVMFLITFFNTRERVQDRSGGKSLPVRESVKAIKGNMPWVAGVL 242 Sbjct: 183 242 Query: 243 IGVVFFILYVVKLSSGIYYITYNLNQ 268 Sbjct: 243 268 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.141 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,564,663 Number of extensions: 533626 Number of successful extensions: 1609 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 1515 Number of HSP's successfully gapped: 39 Length of query: 280 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 175 Effective length of database: 26,268,361 Effective search space: 4596963175 Effective search space used: 4596963175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)