BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0454.0016.p (468 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CC0968 [G] COG1472 Beta-glucosidase-related glycosidases 217 3e-56 CC1756 [G] COG1472 Beta-glucosidase-related glycosidases 190 5e-48 Rv0186 [G] COG1472 Beta-glucosidase-related glycosidases 188 2e-47 MT0195 [G] COG1472 Beta-glucosidase-related glycosidases 188 2e-47 YPO2803 [G] COG1472 Beta-glucosidase-related glycosidases 175 1e-43 >CC0968 [G] COG1472 Beta-glucosidase-related glycosidases Length = 758 Score = 217 bits (553), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 155/494 (31%), Positives = 235/494 (47%), Gaps = 62/494 (12%) Query: 3 HGVHCGHHDYLLNQVLKDEWDFDGFVISDFAWGIK-DTVEAANGGMDVEMC-----HTKF 56 + V+ D+LLN VLK +W + G+V+SD WG + +AAN G+D E F Sbjct: 263 NSVYACESDWLLNTVLKKDWGYKGYVMSD--WGAAHSSAKAANAGLDQESAGDAFDKQPF 320 Query: 57 FGGKLVEAVRNGQVSEEKIDESAVRIVRTLLAFTEADKDTYGKELIASKE----HIALAL 112 F L + +G VS+ +ID+ A R++R + A D G L E H + Sbjct: 321 FAAPLKADLASGAVSQARIDDMARRVLRAMFASGVVDHPLTGAPLAMPAETLAAHAKITQ 380 Query: 113 EAAEKSMTLMKNDHHVLPFSKTETKRIAVIGELGSKGNIGDHGSSRVFPP---------- 162 AE+ + L+KND +LP +KT K IAVIG G + GSS+V+P Sbjct: 381 ADAEEGIVLLKNDGGLLPLAKT-AKTIAVIGSYADVGVLSGGGSSQVYPVGGRAVQGLEP 439 Query: 163 ---------YIVTPLEGIKRLLPNAEVIFDSGEDLEQAKQLAKSVD-AVVFVAGYDHDDE 212 Y +PL+ I P A+V++D G D +A +LA S D A+VF Sbjct: 440 KTWPGPVIYYPSSPLKAIAARYPGAKVVYDDGTDPARAAKLAASSDLALVFA-------- 491 Query: 213 GESVTNEDADLKAGGDRKNSLGLHTKDIELIKAVGPVNPNSAAVLIGGNMIMIEEWKQSV 272 + T E D+ +L L I AV N + VL+ G +++ W V Sbjct: 492 -DQWTTESVDV-------TTLNLPKNQDATIDAVASANKKTVVVLLTGGPLLMP-WLDKV 542 Query: 273 SSILMAYYPGMEGGTAIAKTLFGDVNPGGKLPFVVPVSESHLPQVDWDAT------EIKY 326 +++ A++PG GG AIA+ L G+V+ G+LP P S + LP+ D Sbjct: 543 GAVVEAWFPGTAGGEAIARVLTGEVDASGRLPVTFPKSVAELPRPKLDGLGKPDGEMFDV 602 Query: 327 DYYH-----GYAKLEKEGIEPSLPYGYGLSYTSFALSNASFKTVDDHIIAACEVENTGDV 381 DY GY + + IEP +G+GLSYT FA SN + D + + EV+N G Sbjct: 603 DYTLEGAAVGYKWYDLKKIEPLFAFGHGLSYTQFAYSNLTASASGDTLTVSFEVKNVGRR 662 Query: 382 TGDEVVQLYVGFGNSGLDRPVKVLRGFKRITLKPGQKEKVEIACPVEKIKWFNPAANRWE 441 G +V Q+YVG G + P K L GF++++L PG ++V + + ++ A+ W Sbjct: 663 PGKDVPQVYVGPKAGGWEAP-KRLAGFQKVSLAPGASQRVTVTVDPRLLAVWDSKAHGWS 721 Query: 442 LEEMAYEVYIGTSS 455 + Y++ +G SS Sbjct: 722 IAAGQYDIALGASS 735 >CC1756 [G] COG1472 Beta-glucosidase-related glycosidases Length = 762 Score = 190 bits (482), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 162/519 (31%), Positives = 243/519 (46%), Gaps = 80/519 (15%) Query: 3 HGVHCGHHDYLLNQVLKDEWDFDGFVISDFAWG-IKDTVEAANGGMDV----EMCHTKFF 57 +G +D+LLN+VLK +W++ G+V+SD WG + TV+AA G+D E+ FF Sbjct: 263 NGDWACENDFLLNKVLKRDWNYPGWVMSD--WGAVHSTVKAALAGLDQQSGQELDTQIFF 320 Query: 58 GGKLVEAVRNGQVSEEKIDESAVRIVRTLLAFTEADKDT-YGKELIASKEHIALALEAAE 116 G L AV G+VS+ ++D+ RI+ +++ D T + I H +A AE Sbjct: 321 GEDLKAAVAKGEVSQARVDDMVRRILHGVISSGLMDNPTPTSAQPIDYDAHAKVAQTVAE 380 Query: 117 KSMTLMKNDHHVLPFSKTETKRIAVIGELGSKGNIGDHGSSRV---------FPP----- 162 + L+KND +LP +K+ K+I +IG G I GSS+V P Sbjct: 381 RGSVLLKNDRGLLPLAKS-AKKIVLIGAHADVGVISGGGSSQVRSVGGAPVEIPLKGGEA 439 Query: 163 --------YIVTPLEGIKRLLPNAEVIFDSGEDLEQAKQLAKSVDAVVFVAGYDHDDEGE 214 + +PL+ IK P+AEV + G+D A AK D + A + + Sbjct: 440 ASFVRVTWHASSPLQAIKAANPSAEVTYVDGKDPVAAAAAAKDADVAIVFAWHWQTEA-- 497 Query: 215 SVTNEDADLKAGGDRKNSLGLHTKDIELIKAVGPVNPNSAAVL-IGGNMIMIEEWKQSVS 273 +DA S+ L LI+AV N N+ VL GG ++M W V Sbjct: 498 ----QDAP---------SIALPENQDALIEAVSAANKNAVVVLETGGPVLM--PWLDKVG 542 Query: 274 SILMAYYPGMEGGTAIAKTLFGDVNPGGKLPFVVPVSESHLPQVDW----------DATE 323 ++L A+YPG GG AIA+ LFG+VNP G+L P SE P+ DA Sbjct: 543 AVLQAWYPGQRGGQAIARLLFGEVNPSGRLAMTFPKSEDQAPRASAPGFAEQAAIDDARR 602 Query: 324 ------------IKY--DYYHGYAKLEKEGIEPSLPYGYGLSYTSFALSNASFKTVD-DH 368 ++Y GY +E P P+GYGLSYTSF N K D D Sbjct: 603 AGQKAGPIKGFPVRYVEGAAVGYRWFAQEKRRPLYPFGYGLSYTSFGYKN--LKVEDGDG 660 Query: 369 IIAACEVENTGDVTGDEVVQLYVGFGNSGLDRPVKVLRGFKRITLKPGQKEKVEIACPVE 428 + + +V NTG V G + QLYV SG + + L GF+++ L PG+ ++V + Sbjct: 661 LKVSFDVTNTGKVAGADTPQLYV---TSGQRKAMLRLAGFQKVDLAPGETKRVTLNVEPR 717 Query: 429 KIKWFNPAANRWELEEMAYEVYIGTSSAEKHLIKGSINL 467 + ++ A W + Y +Y+G ++ E ++ GS L Sbjct: 718 ILADYDTAKPGWTIAAGTYPLYVGRNAGEP-VLTGSAKL 755 >Rv0186 [G] COG1472 Beta-glucosidase-related glycosidases Length = 691 Score = 188 bits (478), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 152/502 (30%), Positives = 233/502 (46%), Gaps = 72/502 (14%) Query: 3 HGVHCGHHDYLLNQVLKDEWDFDGFVISDFAWGIKDTVEAANGGMDVE--------MCHT 54 +G + +D+LLN VLK W + G+V+SD WG + E A G+D E + + Sbjct: 205 NGDYAAGNDHLLNDVLKGAWGYRGWVMSD--WGGTPSWECALAGLDQECGAQIDAVLWQS 262 Query: 55 KFFGGKLVEAVRNGQVSEEKIDESAVRIVRTLLAF-TEADKDTYGKELIASKEHIALALE 113 + F +L A +G + + ++ + RI+R++ A + K ++ A E +A + Sbjct: 263 EAFTDRLRAAYADGNLPKGRLSDMVRRILRSMFAVGIDRWKPAPAPDMNAHNE---IAAQ 319 Query: 114 AAEKSMTLMKNDHHVLPFSKTETKRIAVIGELGSKGNIGDHGSSRVFPP----------- 162 A + + L++N +LP + RIAVIG G +GSS V PP Sbjct: 320 MARQGIVLLQN-RGLLPLAPESAGRIAVIGGYAHLGVPAGYGSSAVTPPGGYAGVIPIGG 378 Query: 163 ----------YIV--TPLEGIKRLLPNAEVIFDSGEDLEQAKQLAKSVD-AVVFVAGYDH 209 Y++ +PL +++ LPNA+ FD G + +A A+ D A+VF Sbjct: 379 SGLAAGLRNLYLLPSSPLSELRKRLPNAQFEFDPGINPAEAVLAARRADIAIVFAI---- 434 Query: 210 DDEGESVTNEDADLKAGGDRKNSLGLHTKDIELIKAVGPVNPNSAAVLIGGNMIMIEEWK 269 EGE + D L G D LI AV N N+ VL GN + + W+ Sbjct: 435 RAEGEGFDSADLSLPWGQD------------ALIAAVASANANTVVVLETGNPVTMP-WR 481 Query: 270 QSVSSILMAYYPGMEGGTAIAKTLFGDVNPGGKLPFVVPVSESHLPQ-------VDW-DA 321 SV++I+ A+YPG GG A+A+ + G VNP G+LP PV P+ W + Sbjct: 482 DSVNAIMQAWYPGQAGGQAVAEIVTGQVNPSGRLPITFPVDLGQTPRSQPPELGAPWGTS 541 Query: 322 TEIKYDYYH--GYAKLEKEGIEPSLPYGYGLSYTSFALSNASFKTVDDHII-AACEVENT 378 T I Y GY P +G+GLSYTSF + H + A+ V NT Sbjct: 542 TTIHYTEGADVGYRWFASTNQTPMFAFGHGLSYTSFEYRD--LVVTGGHTVHASFSVTNT 599 Query: 379 GDVTGDEVVQLYVGFGNSGLDRPVKVLRGFKRITLKPGQKEKVEIACPVEKIKWFNPAAN 438 GD +G +V QLY+ G R +++L GF+R+ L+PGQ +V I + ++ A Sbjct: 600 GDRSGADVPQLYM-IAAPGESR-LRLL-GFERVELEPGQTRRVRIEADPRLLARYDGEAR 656 Query: 439 RWELEEMAYEVYIGTSSAEKHL 460 W +E Y V +G S+ L Sbjct: 657 SWRIEPGGYTVAVGASAVALKL 678 >MT0195 [G] COG1472 Beta-glucosidase-related glycosidases Length = 691 Score = 188 bits (478), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 152/502 (30%), Positives = 233/502 (46%), Gaps = 72/502 (14%) Query: 3 HGVHCGHHDYLLNQVLKDEWDFDGFVISDFAWGIKDTVEAANGGMDVE--------MCHT 54 +G + +D+LLN VLK W + G+V+SD WG + E A G+D E + + Sbjct: 205 NGDYAAGNDHLLNDVLKGAWGYRGWVMSD--WGGTPSWECALAGLDQECGAQIDAVLWQS 262 Query: 55 KFFGGKLVEAVRNGQVSEEKIDESAVRIVRTLLAF-TEADKDTYGKELIASKEHIALALE 113 + F +L A +G + + ++ + RI+R++ A + K ++ A E +A + Sbjct: 263 EAFTDRLRAAYADGNLPKGRLSDMVRRILRSMFAVGIDRWKPAPAPDMNAHNE---IAAQ 319 Query: 114 AAEKSMTLMKNDHHVLPFSKTETKRIAVIGELGSKGNIGDHGSSRVFPP----------- 162 A + + L++N +LP + RIAVIG G +GSS V PP Sbjct: 320 MARQGIVLLQN-RGLLPLAPESAGRIAVIGGYAHLGVPAGYGSSAVTPPGGYAGVIPIGG 378 Query: 163 ----------YIV--TPLEGIKRLLPNAEVIFDSGEDLEQAKQLAKSVD-AVVFVAGYDH 209 Y++ +PL +++ LPNA+ FD G + +A A+ D A+VF Sbjct: 379 SGLAAGLRNLYLLPSSPLSELRKRLPNAQFEFDPGINPAEAVLAARRADIAIVFAI---- 434 Query: 210 DDEGESVTNEDADLKAGGDRKNSLGLHTKDIELIKAVGPVNPNSAAVLIGGNMIMIEEWK 269 EGE + D L G D LI AV N N+ VL GN + + W+ Sbjct: 435 RAEGEGFDSADLSLPWGQD------------ALIAAVASANANTVVVLETGNPVTMP-WR 481 Query: 270 QSVSSILMAYYPGMEGGTAIAKTLFGDVNPGGKLPFVVPVSESHLPQ-------VDW-DA 321 SV++I+ A+YPG GG A+A+ + G VNP G+LP PV P+ W + Sbjct: 482 DSVNAIMQAWYPGQAGGQAVAEIVTGQVNPSGRLPITFPVDLGQTPRSQPPELGAPWGTS 541 Query: 322 TEIKYDYYH--GYAKLEKEGIEPSLPYGYGLSYTSFALSNASFKTVDDHII-AACEVENT 378 T I Y GY P +G+GLSYTSF + H + A+ V NT Sbjct: 542 TTIHYTEGADVGYRWFASTNQTPMFAFGHGLSYTSFEYRD--LVVTGGHTVHASFSVTNT 599 Query: 379 GDVTGDEVVQLYVGFGNSGLDRPVKVLRGFKRITLKPGQKEKVEIACPVEKIKWFNPAAN 438 GD +G +V QLY+ G R +++L GF+R+ L+PGQ +V I + ++ A Sbjct: 600 GDRSGADVPQLYM-IAAPGESR-LRLL-GFERVELEPGQTRRVRIEADPRLLARYDGEAR 656 Query: 439 RWELEEMAYEVYIGTSSAEKHL 460 W +E Y V +G S+ L Sbjct: 657 SWRIEPGGYTVAVGASAVALKL 678 >YPO2803 [G] COG1472 Beta-glucosidase-related glycosidases Length = 793 Score = 175 bits (443), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 140/491 (28%), Positives = 225/491 (45%), Gaps = 53/491 (10%) Query: 3 HGVHCGHHDYLLNQVLKDEWDFDGFVISDFAWGIKDTVEAANGGMDVEMCHTKFFGGKL- 61 +G + +LL VL+ +W FDGFV+SD+ +G+ D V A GG D+ M + L Sbjct: 235 NGTPSSQNPWLLKDVLRQQWKFDGFVMSDW-YGVADPVSALKGGNDLNMPGGRTPDDSLF 293 Query: 62 -----------VEAVRNGQVSEEKIDESAVRIVRTLLAFTEADKDTYGKELIASKEHIAL 110 + A+++G++++++IDE+ +R + ++ T K+ + +H AL Sbjct: 294 LTPNTDPKDVVLAALKSGELTQDQIDEN-IRNILNVVIKTHRFKNGDAAMMPEPVDHSAL 352 Query: 111 ALEAAEKSMTLMKNDHHVLPFSKTETKRIAVIGELGSKGNIGDHGSSRVF--PPYIVTPL 168 A + A SM L+KND H LP R+A G+ + GS+ V P +VT Sbjct: 353 ARQIAADSMVLLKNDAHTLPIKAA--FRVAAFGQNIDNFFVTGGGSAEVNIDPKRLVTLP 410 Query: 169 EGIKRLLPNAEVIFDSGEDLEQAKQLAKSVDAVVFVAGYDHDDE-----GESVTNEDADL 223 +G+K +I G+ + L + V+ D G S T Sbjct: 411 QGLKNA--GIALIRQIGD-----QALDEPVNQPALTKAAQQSDIALISIGRSSTE----- 458 Query: 224 KAGGDRKNSLGLHTKDIELIKAVGPV----NPNSAAVLIGGNMIMIEEWKQSVSSILMAY 279 G DR S+ +H ++ +IK V N +L G+ I + W+Q +IL+ + Sbjct: 459 --GADRY-SMAMHPDEVAMIKKVASAFHQQNKKVVVLLNIGSPIEMTSWEQYADAILLTW 515 Query: 280 YPGMEGGTAIAKTLFGDVNPGGKLPFVVPVSESHLPQV---DWDATEIKYD--YYHGYAK 334 PG + G A+A L G VNP GKLP P P +A + Y Y GY Sbjct: 516 QPGEQAGNAVADILLGKVNPSGKLPLTFPKRLEDSPSFGNYPGNAKTVIYGEGIYVGYRY 575 Query: 335 LEKEGIEPSLPYGYGLSYTSFALSNASFKT------VDDHIIAACEVENTGDVTGDEVVQ 388 + + I P P+GYGLSY++ S + ++ I + V NT + EVVQ Sbjct: 576 FDTKRIAPMYPFGYGLSYSTVNYGKISPEKPVFNIDTENSIEVSIPVRNTSGIDTKEVVQ 635 Query: 389 LYVGFGNSGLDRPVKVLRGFKRITLKPGQKEKVEIACPVEKIKWFNPAANRWELEEMAYE 448 LYV S LDRP + L+ F++++L G++++V +++ N W LE + Sbjct: 636 LYVHDNASRLDRPEQELKAFEKVSLSAGEEKRVAFKLDKRAFSYYDEDKNNWVLEPGLFT 695 Query: 449 VYIGTSSAEKH 459 + IG SS + H Sbjct: 696 LRIGRSSRDIH 706 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,954,166 Number of extensions: 1695173 Number of successful extensions: 2584 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 2487 Number of HSP's successfully gapped: 30 Length of query: 468 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 358 Effective length of database: 25,691,691 Effective search space: 9197625378 Effective search space used: 9197625378 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 118 (50.1 bits)