BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0454.0006.p (433 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1117 [G] COG1653 ABC-type sugar transport system, periplasmic ... 120 5e-27 BS_yesO [G] COG1653 ABC-type sugar transport system, periplasmic... 119 1e-26 YPO1719 [G] COG1653 ABC-type sugar transport system, periplasmic... 112 2e-24 all1027 [G] COG1653 ABC-type sugar transport system, periplasmic... 69 1e-11 PH0753 [G] COG1653 ABC-type sugar transport system, periplasmic ... 66 9e-11 >BH1117 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 434 Score = 120 bits (300), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 94/343 (27%), Positives = 156/343 (45%), Gaps = 15/343 (4%) Query: 29 GTANSAADTVELRFAWWGSEDRHTATLEAIKIFEEKHPGIKILPEYSGFDGYESKIQAQI 88 G + + V LR AWWG ++RH T+EAI++FEEK+P I++ PE++ +D Y ++ Q Sbjct: 33 GEQEAGNEDVVLRVAWWGGQERHDMTIEAIELFEEKYPHIQVDPEFTSWDNYWERLTTQA 92 Query: 89 AGNSAPDLFQ----STGEINVR---LGADSLLPLDGVLDTTGMSDSIMESVAMDGKTVGA 141 AGN+ PD+ Q E + R + SL+ DG ++ + D + DG Sbjct: 93 AGNNLPDVIQMDNSKLNEYSSRGLVIDLQSLIA-DGTINLDDVDDVYQDMNVQDGSVWAV 151 Query: 142 YIGLAGDAYLYDETLLKQLGVEPPVPPYSWDDLAAKFKEIAEKSGGKVYGAVDSSVAFDI 201 G A +Y+E +L++ G++ P Y+++D + + E G YG + +++ Sbjct: 152 SAGSNALAAIYNEEMLEEQGIQLE-PGYTYEDFYEAMQTLKENIDGDFYGYDFGNAEYEM 210 Query: 202 FKNYGITALDASAPFPNSD-SAFTFTEEQIRSYYQYWDDLRKAGAVAPPDLSATSDDSAN 260 F Y A F N D + F + + + + + G P L+ Sbjct: 211 FFVY---ARQNGESFYNEDGTGLGFENQTLIDFLTFVKQMVDDGVAPPHALTLEYIQGGE 267 Query: 261 SLLVKGKAAFVPIASSSFSRLQSQTKNTLNMIMLPQSAKTGVTVTPGTSQVLVASAKTKH 320 SLL A AS+ LQ T++ L +++LP S GV + + + Sbjct: 268 SLLGDQMAGAAMAASNGIIGLQQSTEHQLGLMLLP-SLDGGVHGNWIRPSMSYSISSHSE 326 Query: 321 PQEVAE-FINFFVHDTDAANALKTTRGVLPTTQQQEALLANAD 362 QE A FI+F +D A LK RGV +++ +E L D Sbjct: 327 EQEAAALFIDFLTNDLQANEILKAERGVPISSKVREHLAPMVD 369 >BS_yesO [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 412 Score = 119 bits (297), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 91/335 (27%), Positives = 160/335 (47%), Gaps = 18/335 (5%) Query: 21 CSKAEPDAGTANSAADTVELRFAWWGSEDRHTATLEAIKIFEEKHPGIKILPEYSGFDGY 80 CS E +G + V LR AWWG + RH T + I+++E+K+P + I E++ +D Y Sbjct: 5 CSAGEEASGKK----EDVTLRIAWWGGQPRHDYTTKVIELYEKKNPHVHIEAEFANWDDY 60 Query: 81 ESKIQAQIAGNSAPDLFQSTGEINVRLGA----DSLLPL--DGVLDTTGMSDSIMESVAM 134 K+ A PD+ Q + G + L P DG +D + + ++++ + Sbjct: 61 WKKLAPMSAAGQLPDVIQMDTAYLAQYGKKNQLEDLTPYTKDGTIDVSSIDENMLSGGKI 120 Query: 135 DGKTVGAYIGLAGDAYLYDETLLKQLGVEPPVPPYSWDDLAAKFKEIAEKSGGKVYGAVD 194 D K G +G+ + + +E LLK+ GV ++W+D ++ EK+G VYG+ + Sbjct: 121 DNKLYGFTLGVNVLSVIANEDLLKKAGVSINQENWTWEDYEKLAYDLQEKAG--VYGS-N 177 Query: 195 SSVAFDIFKNYGITALDASAPFPNSDSAFTFTEEQI-RSYYQYWDDLRKAGAVAPPDLSA 253 DIF Y + + + + ++Q+ Y++ L KA PD SA Sbjct: 178 GMHPPDIFFPYYLRT-KGERFYKEDGTGLAYQDDQLFVDYFERQLRLVKAKTSPTPDESA 236 Query: 254 TSDDSANSLLVKGKAAFVPIASSSFSRLQSQTKNTLNMIMLP-QSAKTGVTVTPGTSQVL 312 + +VKGK+A S+ + T + L++ + P Q + +T+ P S + Sbjct: 237 QIKGMEDDFIVKGKSAITWNYSNQYLGFARLTDSPLSLYLPPEQMQEKALTLKP--SMLF 294 Query: 313 VASAKTKHPQEVAEFINFFVHDTDAANALKTTRGV 347 ++H +E A+FINFFV++ +A +K RGV Sbjct: 295 SIPKSSEHKKEAAKFINFFVNNEEANQLIKGERGV 329 >YPO1719 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 430 Score = 112 bits (279), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 104/415 (25%), Positives = 176/415 (42%), Gaps = 31/415 (7%) Query: 31 ANSAADTVELRFAWWGSEDRHTATLEAIKIFEEKHPGIKILPEYSGFDGYESKIQAQIAG 90 A +A D V LR +WWG RH TL+A++ F ++HP I + EY+G+DG+ S++ QIAG Sbjct: 21 AFAAQDDVNLRMSWWGGNGRHQVTLKALEEFHKQHPNINVKAEYTGWDGHLSRLTTQIAG 80 Query: 91 NSAPDLFQSTGEINVRLGAD--SLLPLDGV---LDTTGMSDSIMESVAMDGKTVGAYIGL 145 + PD+ Q+ D L+ V LD ++ ++GK G I + Sbjct: 81 GTEPDVMQTNWNWLPIFSKDGTGFFDLNNVKEQLDLAQFDPIELQQTTVNGKLNGIPISV 140 Query: 146 AGDAYLYDETLLKQLGVEPPVPPYSWDDLAAKFKEIAEKSGGKVYGAV-DSSVAFDIFKN 204 + +++ + G+E P +WD+L + EK G + Y V + + ++ Sbjct: 141 TARIFYFNDATWAKAGLEYPK---TWDELLNAGQVFKEKLGDQYYPVVLEHQDTLALIRS 197 Query: 205 YGITALDASAPFPNSDSA---FTFTEEQIRSYYQYWDDLRKAGAVAPPDLSATSDDSANS 261 Y + P D A F +T EQ ++ + + + V P S +N Sbjct: 198 Y----MTQKYNIPTVDEANKKFAYTPEQWVEFFTMYKKMVDS-HVMPSSKYYASFGKSNM 252 Query: 262 LLVK----GKAAFVPIASSSFSRLQSQTKNTLNM-----IMLPQSAKTGVTVTPGTSQVL 312 +K G+ A + +S+ ++ + MLP + G+ P +Q+L Sbjct: 253 YEMKPWINGEWAGTYMWNSTITKYSDNLTKPAKLDLGPYPMLPDAKDAGLFFKP--AQML 310 Query: 313 VASAKTKHPQEVAEFINFFVHDTDAANALKTTRGVLPTTQQQEALLANADLSEGDKKNID 372 TKHP+E A INF ++ + +AL RGV + L AN + + D Sbjct: 311 SIGKSTKHPKESAMLINFLLNSKEGVDALGLERGVPLSAAAVAQLRANGVIKDEDPSVAG 370 Query: 373 LIQQLNKLEXXXXXXXXXXXQVLGWKGLIEKVGQEIAFGKISVDDGAKKLMKEVD 427 L L Q++ L Q I +G+ SV++ A+ K+ D Sbjct: 371 LNMALELPHEMKTSPYFDDPQIV---SLFGDAIQYIDYGQKSVEETAEYFNKQGD 422 >all1027 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 432 Score = 69.3 bits (168), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 94/384 (24%), Positives = 160/384 (41%), Gaps = 44/384 (11%) Query: 21 CSKAEPDAGTANSAADTVELRFAWWGSEDRHTATLEA-IKIFEEKHPGIKILPEYSGFDG 79 CS + T +A+ T + F + + T ++ I FE ++PGIK+ + Sbjct: 26 CSTGNVNTNTQQTASGTANIEFWTMQLQPQFTNYFQSLIANFETQNPGIKVNWVDVPWAA 85 Query: 80 YESKIQAQIAGNSAPDLFQSTGEINVRL-GADSLLPLDGVLDTTGMSD---SIMESVAMD 135 E+KI ++ + PD+ + +L G ++ L LD + S +I ++ ++ Sbjct: 86 MENKILTAVSAKTPPDVVNLNPDFASQLAGRNAWLDLDTKVPQEARSSYLPNIWKASTLN 145 Query: 136 GKTVGAYIGLAGDAYLYDETLLKQLGVEPPVPPYSWDDLAAKFKEIAEKSGGKVYGAV-- 193 GK+ G L +Y+ LLKQ G+ PP ++ +LA ++I +K+G + Sbjct: 146 GKSFGVPWYLTTRLTIYNTDLLKQAGISK--PPATYAELAQAAQQIKDKTGKYAFFVTFV 203 Query: 194 --DSSVAFDIFKNYGITALDASAPFPNSDSAFTFTEEQIRSYYQYWDDLRKAGAVAPPDL 251 DS F G T +D ++ F Q ++ +QYW DL K G + P + Sbjct: 204 PQDSGEVLQSFVQMGATLVD-------TEGKAAFNSPQGKAAFQYWVDLYKKG-LLPKEA 255 Query: 252 SATSDDSANSLLVKGKAAFVPIASSSFSRLQSQTKNTLNM----IMLPQ----SAKTGVT 303 A L G+ AF+ +S L++ T N + + PQ + K V Sbjct: 256 LTQGHRHAIDLYQSGETAFL---ASGPEFLKTITTNAPKVAQASAIAPQLTGDTGKKNVA 312 Query: 304 VTPGTSQVLVASAKTKHPQEVAEFINFFVHDTD------AANALKTTRGVLPTTQQQEAL 357 V +V TK P +F F +D + AAN L +T L + ++ Sbjct: 313 VMN-----IVVPRDTKQPDAAVKFALFVTNDENQLAFAKAANVLPSTTKALADSYFKD-- 365 Query: 358 LANADLSEGDKKNIDLIQQLNKLE 381 AD S +K + QQL + E Sbjct: 366 -IPADASTVEKARVVSAQQLQQAE 388 >PH0753 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 464 Score = 66.2 bits (160), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 81/321 (25%), Positives = 134/321 (41%), Gaps = 36/321 (11%) Query: 36 DTVELRFAWWGSEDRHTATLE-AIKIFEEKHPGIKILPEYSGFDGYESKIQAQIAGNSAP 94 + V +RFA W + + + I++FE+++P IK+ E + I A AP Sbjct: 66 NQVFIRFAGWSAGETEMKNYQRMIELFEKENPNIKVKYEVIT-QMFHENILASFGAGVAP 124 Query: 95 DLF---QSTGEINVRLGADSLLPLDGVLDTTGMSDS---IMESVAMDGKTVGAYIGLAGD 148 D+F S I + GA L P+ + D + ++ ++E +GK G + Sbjct: 125 DVFYVDSSWAPIFIEKGA--LYPISELADESFINKFYPFLLEPFKKNGKLYGLPKDWSML 182 Query: 149 AYLYDETLLKQLGVEPPVPPYSWDDLAAKFKEIAEKSGGKVYGAVDSSVAFDIFKNYGIT 208 A Y++ L +Q G+ P P +WD+L K I EK+G ++ F Y Sbjct: 183 ALFYNKKLFEQAGLTKP--PETWDELLEYAKIITEKTGKP-----GLAIYLGGFNRYVPV 235 Query: 209 ALDASAP---FPNSDSAFTFTEEQIRSYYQYWDDLRKAGAVA------PPDLSATSDDSA 259 A+ AP F + A F +R ++ +L + G V PP + A D A Sbjct: 236 AVSNGAPKPWFEKPEDASWFDNPVVRETLTWYINLYRKGKVEREKKGLPPYVVAPEDVGA 295 Query: 260 NSL---LVKGKAAFVPIASSSFSRLQSQTKNT-----LNMIMLPQSAKTGVTVTPGTSQV 311 L + K A V + L Q N ++ LP VT+ + + Sbjct: 296 GWLGDAFGQQKVAMVISGNWMIPFLADQFPNFKYGEDWDIAPLPAGKNGRVTMV--YTVI 353 Query: 312 LVASAKTKHPQEVAEFINFFV 332 L ++KTKHP+E +F+ F + Sbjct: 354 LGINSKTKHPKEAWKFVEFIL 374 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.130 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,095,310 Number of extensions: 1169174 Number of successful extensions: 2790 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 2795 Number of HSP's successfully gapped: 14 Length of query: 433 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 324 Effective length of database: 25,807,025 Effective search space: 8361476100 Effective search space used: 8361476100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)