BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0451.0002.p (310 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yulC [G] COG1070 Sugar (pentulose and hexulose) kinases 197 1e-50 TM1073 [G] COG1070 Sugar (pentulose and hexulose) kinases 177 2e-44 STM1073 [S] COG1289 Predicted membrane protein 177 2e-44 lin2981 [G] COG1070 Sugar (pentulose and hexulose) kinases 176 5e-44 BH1551 [G] COG1070 Sugar (pentulose and hexulose) kinases 174 1e-43 >BS_yulC [G] COG1070 Sugar (pentulose and hexulose) kinases Length = 485 Score = 197 bits (502), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 109/296 (36%), Positives = 162/296 (54%), Gaps = 4/296 (1%) Query: 9 AVDLGASSGRVILGTYDGSRIVTEELHRFANTPVEAGGHLYWNVPELLKEIKQGILLAVQ 68 A+D+GASSGR+++G + ++ +E+HRFAN + GH W++ LLK+I QG+ Sbjct: 5 AIDVGASSGRIMVGELNEGKLDIQEIHRFANGFSQRDGHCLWDIDHLLKQILQGLQKVKT 64 Query: 69 AGNEIASLSVDTWGVDYGFVDHAGKLLNAPHHYRDQRVGKYRARXXXXXXXXXQFRLTGN 128 G E ++ +DTW VDY +D G L YRD+R + ++ TG Sbjct: 65 LGYEHCTVGIDTWAVDYVLLDEKGDRLREAISYRDRRTDHTIDKLEHTLSKAAIYQKTGI 124 Query: 129 QPDPINTVYQLFADLQENPLLQEQVDHILMMPDLFLYLLSGASSAERTILSTSGLLDAVT 188 Q P NT+YQLF +E+ L ++ D I+M+PD Y L+G + E T +ST+ LL+ T Sbjct: 125 QFQPFNTIYQLF---EEDRELLKKTDKIMMIPDYLGYCLTGKAVTEITNVSTTQLLNVST 181 Query: 189 GEPSSEVFGRLGIPTRLIPPRVEAGTAIGTLRPELCAKLGCGPIRVIAGASHDTASAVAS 248 G E+ + + + P E G +G LR E +V+ A+HDTASAV + Sbjct: 182 GNLDPELLEAVSVLEQQFAPLTEPGCELGKLRNEWFPDYDLPACKVMTVATHDTASAVIA 241 Query: 249 IPYADKDGAAFISCGTWSLVGMETKAPVITALSYEYGFTNEGCSGSGNRLLXXXXG 304 P + DG A+IS GTWSL+G+E K P+IT L+ E +TNE + + R L G Sbjct: 242 APGVN-DGWAYISSGTWSLIGVENKTPIITDLALENNYTNERGANNTIRFLKNIIG 296 >TM1073 [G] COG1070 Sugar (pentulose and hexulose) kinases Length = 476 Score = 177 bits (448), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 111/293 (37%), Positives = 158/293 (53%), Gaps = 19/293 (6%) Query: 6 KLLAVDLGASSGRVILGTYDGSRIVTEELHRFANT----PVEAGGHLYWNVPELLKEIKQ 61 K+L++DLGA++G+ I R+ +E+ RF P + H WN+P +EIK Sbjct: 4 KVLSIDLGATNGK-IYEVELKDRLTVKEIKRFRTEGTFLPGKDREHFVWNLPGFYEEIK- 61 Query: 62 GILLAVQAGNEIASLSVDTWGVDYGFVDHAGKLLNAPHHYRDQRVGKYRARXXXXXXXXX 121 G+L A A S+ VDTWGVD+ +D G+L++ P+HYRD R + Sbjct: 62 GVLEASDA----QSVGVDTWGVDFALLDENGRLVSLPYHYRDVRTKGVMKKAFEVVPKDE 117 Query: 122 QFRLTGNQPDPINTVYQLFADLQENPLLQEQVDHILMMPDLFLYLLSGASSAERTILSTS 181 F+ TG Q INT+YQL++ + N + H+LM+PD+F + LSG +E TI STS Sbjct: 118 IFQRTGIQFMEINTLYQLYSMVLSNDPFLKTTKHLLMIPDVFNFWLSGEMVSEYTIASTS 177 Query: 182 GLLDAVTGEPSSEVFGRLGIPTRLIPPRVEAGTAIGTLRPELCAKLGCGPIRVIAGASHD 241 GE + ++ +L IPT + P V GT +G R K G I VIA A HD Sbjct: 178 QCYSVPDGEWAYDLLEKLSIPTEIFPKVVPPGTILGKCR----VKKG---INVIATACHD 230 Query: 242 TASAVASIPYADKDGAAFISCGTWSLVGMETKAPVITALSYEYGFTNEGCSGS 294 TASAV ++P + +IS GTW LVG E + P+++ + E FTNEG G Sbjct: 231 TASAVVAVPLEGE--GIYISSGTWFLVGTELERPLLSKEALERNFTNEGGYGK 281 >STM1073 [S] COG1289 Predicted membrane protein Length = 717 Score = 177 bits (448), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 21/293 (7%), Positives = 46/293 (15%), Gaps = 19/293 (6%) Query: 6 KLLAVDLGASSGRVILGTYDGSRIVTEELHRFANT----PVEAGGHLYWNVPELLKEIKQ 61 L+ S+ + T L + G + + +L + Sbjct: 4 PLIRRYTWNSTWL-YYIRIFIALCGTTALPWWLGDVKLTIPLTLGMVAAALTDLDDRLA- 61 Query: 62 GILLAVQAGNEIASLSVDTWGVDYGFVDHAGKLLNAPHHYRDQRVGKYRARXXXXXXXXX 121 G L + + + L Sbjct: 62 GRLRNLII----TLICFFIASASVELLFPWPWLFALGLTLSTSGFILLGGLGQRYATIAF 117 Query: 122 QFRLTGNQPDPINTVYQLFADLQENPLLQEQVDHILMMPDLFLYLLSGASSAERTILSTS 181 L ++Y + L ++L + L+ + Sbjct: 118 GALLIAIYTMLGTSLYDHWYQQPLLLLAGAVWYNLLTLTGHLLFPIRPLQDNLARSYEQL 177 Query: 182 GLLDAVTGEPSSEVFGRLGIPTRLIPPRVEAGTAIGTLRPELCAKLGCGPIRVIAGASHD 241 + + K+ + Sbjct: 178 AHYLELKSRLFDPDIEDESQAPLYDLALANGQLMATLNQ----TKVS---LLTRLRGDRG 230 Query: 242 TASAVASIPYADKDGAAFISCGTWSLVGMETKAPVITALSYEYGFTNEGCSGS 294 ++ Y A I S S S Sbjct: 231 QRGTRRTLHYYFV--AQDIHERASSSHIQYQTLRDYFRHSDVMFRFQRLMSMQ 281 >lin2981 [G] COG1070 Sugar (pentulose and hexulose) kinases Length = 483 Score = 176 bits (445), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 106/301 (35%), Positives = 158/301 (52%), Gaps = 4/301 (1%) Query: 4 LKKLLAVDLGASSGRVILGTYDGSRIVTEELHRFANTPVEAGGHLYWNVPELLKEIKQGI 63 +K +AVD+GASSGR+ILG ++ EE+HRF N GH W + +L++EI G+ Sbjct: 1 MKHYVAVDIGASSGRLILGKLVNEKLQLEEIHRFKNGFTYRDGHERWEIDQLMQEIFIGL 60 Query: 64 LLAVQAGNEIASLSVDTWGVDYGFVDHAGKLLNAPHHYRDQRVGKYRARXXXXXXXXXQF 123 Q G L +DTWGVDY + +G+ L P YRD+R + Sbjct: 61 EKVKQLGISECVLGIDTWGVDYVLIGASGEKLADPISYRDKRTLNAVQNLTSEYPREYIY 120 Query: 124 RLTGNQPDPINTVYQLFADLQENPLLQEQVDHILMMPDLFLYLLSGASSAERTILSTSGL 183 + TG Q +NT+YQL+ ++E LL E+ + IL++PD Y+L+G AE T ST+ + Sbjct: 121 KKTGIQFMELNTLYQLY--VEERNLL-ERAEKILLIPDYIGYVLTGVKVAETTNSSTTQM 177 Query: 184 LDAVTGEPSSEVFGRLGIPTRLIPPRVEAGTAIGTLRPELCAKLGCGPIRVIAGASHDTA 243 L+ ++ L I P +AGT +G ++ E + V+ A+HDTA Sbjct: 178 LNLREQLFDKDLLSHLNIDVEKFAPLTDAGTYLGKVKAEWLEEYDIPNCDVVTVATHDTA 237 Query: 244 SAVASIPYADKDGAAFISCGTWSLVGMETKAPVITALSYEYGFTNEGCSGSGNRLLXXXX 303 SAV P A+ + AF+S GTWSL+GME AP+ +++ +TNE + R L Sbjct: 238 SAVVGTP-AEGENWAFLSSGTWSLIGMELIAPINNEAAFKENYTNEWGAYGTYRFLKNIM 296 Query: 304 G 304 G Sbjct: 297 G 297 >BH1551 [G] COG1070 Sugar (pentulose and hexulose) kinases Length = 467 Score = 174 bits (441), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 103/298 (34%), Positives = 154/298 (51%), Gaps = 6/298 (2%) Query: 8 LAVDLGASSGRVILGTYDGSRIVTEELHRFANTPVEAGGHLYWNVPELLKEIKQGILLAV 67 LAVD+GASSGR+I+G + +I E+HRF N +E G W+V L EIK G+ Sbjct: 5 LAVDIGASSGRLIVGECN-KKIQLTEIHRFENQIIEKNGQFCWDVDALFSEIKTGLKKCR 63 Query: 68 QAGNEIASLSVDTWGVDYGFVDHAGKLLNAPHHYRDQRVGKYRARXXXXXXXXXQFRLTG 127 +AG E S+ +DTW VD+ +D K L YRD R + TG Sbjct: 64 EAGIEPVSMGIDTWAVDFVLLDEHDKPLTDAVSYRDPRTDGVMEEVIEQFMKERLYLETG 123 Query: 128 NQPDPINTVYQLFADLQENPLLQEQVDHILMMPDLFLYLLSGASSAERTILSTSGLLDAV 187 Q NT+YQL+A +++P + ++ LM+PD F +LL+G + E T +T+ L++A Sbjct: 124 IQFQQFNTIYQLYALKKQHPDIFKKAKSFLMIPDYFHFLLTGKKANEYTNATTTQLVNAF 183 Query: 188 TGEPSSEVFGRLGIPTRLIPPRVEAGTAIGTLRPELCAKLGCGPIRVIAGASHDTASAVA 247 T + ++ LG + ++G L+ E ++G + VI A+HDT SAV Sbjct: 184 TKKWDKDIIEALGFNPDMFQEIKLPTESLGKLKSEWVEEVGFD-LEVILPATHDTGSAVV 242 Query: 248 SIP-YADKDGAAFISCGTWSLVGMETKAPVITALSYEYGFTNEGCSGSGNRLLXXXXG 304 ++P AD ++S GTWSL+G+E P+ + +Y FTNEG R L G Sbjct: 243 AVPKVAD---TIYLSSGTWSLIGVENSFPICVTKALDYNFTNEGGMNYQFRFLKNIMG 297 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,484,543 Number of extensions: 986453 Number of successful extensions: 3094 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 3053 Number of HSP's successfully gapped: 28 Length of query: 310 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 204 Effective length of database: 26,153,027 Effective search space: 5335217508 Effective search space used: 5335217508 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)