BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0025.p (582 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yheI [V] COG1132 ABC-type multidrug transport system, ATPase ... 706 0.0 ZmdlA [V] COG1132 ABC-type multidrug transport system, ATPase an... 427 e-119 mdlA [V] COG1132 ABC-type multidrug transport system, ATPase and... 427 e-119 ZmdlA [V] COG1132 ABC-type multidrug transport system, ATPase an... 427 e-119 ECs0502 [V] COG1132 ABC-type multidrug transport system, ATPase ... 427 e-119 >BS_yheI [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 585 Score = 706 bits (1821), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/575 (57%), Positives = 431/575 (74%) Query: 1 LFSVLRNLGWFFRREKKRYXXXXXXXXXXXXXXXXPPRLLGNAIDDIVNGSITAGSLMKY 60 +FSVL+ LGWFF+ RY PP+LLGNAIDD+ G+ TA L+ Y Sbjct: 1 MFSVLKKLGWFFKAYWLRYTIAIVLLLAVNVIEMFPPKLLGNAIDDMKAGAFTAEGLLFY 60 Query: 61 IGLIVLMLLIIYWITYIWMHKLFGGSNLVERLLRSRFMNHLMTMTPSFFERNRTGDLMAR 120 IG+ ++ +Y ++Y WMH+LFGG+NL+E++LR++ M HL+TM+P F+E+NRTGDLMAR Sbjct: 61 IGIFFVLTAAVYIMSYFWMHQLFGGANLMEKILRTKLMGHLLTMSPPFYEKNRTGDLMAR 120 Query: 121 ATNDIRAVSATVGFGMLTLVDSTIFLTVVLLAMGFLVSWKLTXXXXXXXXXXXXXMIFYG 180 TND++AVS T GFG+LTLVDST+F+ + L MGFL+SWKLT + YG Sbjct: 121 GTNDLQAVSLTTGFGILTLVDSTMFMMTIFLTMGFLISWKLTFAAIIPLPVMAIAISLYG 180 Query: 181 KAIHDRYSLAQDAFGDMNDQVLESVSGIRVIRAYVQERLDEKRFGEITEDVYRKNMAVAK 240 IH+R++ AQ+AFG +ND+VLESVSG+RVIRAYVQE D +RF E+T DVY+KNM VA Sbjct: 181 SKIHERFTEAQNAFGALNDRVLESVSGVRVIRAYVQETNDVRRFNEMTADVYQKNMKVAF 240 Query: 241 VDSFFEPTIRLCVGLSYVIALTFGIYLVFRNQITLGDLVSFNMYLGMIVWPMFAIGELIN 300 +DS FEPT++L VG SY+I L +G +LVFRN++TLG+LVSFN+YLGM++WPMFAIGELIN Sbjct: 241 IDSLFEPTVKLLVGASYLIGLGYGAFLVFRNELTLGELVSFNVYLGMMIWPMFAIGELIN 300 Query: 301 IMQRGGASLERIDETLNSKPDVQDAALPVPVAQPTSIELKDVTFRYPTSTIDNLTGVSFT 360 +MQRG ASL+R++ETL+ + DV D P + +P I V+F YP+ST DNL +SFT Sbjct: 301 VMQRGNASLDRVNETLSYETDVTDPKQPADLKEPGDIVFSHVSFTYPSSTSDNLQDISFT 360 Query: 361 LTQGQTLGVVGRTGSGKSTLLKQLLHEYPTGRGEILISDVPIDKIALDQLHSWMGYVPQE 420 + +GQT+G+ G+TGSGK+T++KQLL +YP G G I S VPI +I LD+L W+GYVPQ+ Sbjct: 361 VRKGQTVGIAGKTGSGKTTIIKQLLRQYPPGEGSITFSGVPIQQIPLDRLRGWIGYVPQD 420 Query: 421 QILFSKSVRENIQFGHHGASDERIMSAITAAAFQNXXXXXXXXXXXXVGERGVSLSGGQK 480 +LFS++V+ENI +G A+D+ + AI A F+ VGE+GV+LSGGQK Sbjct: 421 HLLFSRTVKENILYGKQDATDKEVQQAIAEAHFEKDLHMLPSGLETMVGEKGVALSGGQK 480 Query: 481 QRVSISRAFIANPDILILDDALSAVDARTEARIIENIRDERAGKTTLISTHRLSAIEHAD 540 QR+SI+RA +ANP+ILILDD+LSAVDA+TEA II+NIR+ R GKTT I THRLSA+EHAD Sbjct: 481 QRISIARALMANPEILILDDSLSAVDAKTEAAIIKNIRENRKGKTTFILTHRLSAVEHAD 540 Query: 541 LIVVLDNGKIIERGTHQELLDMNGWYREQFDRQQV 575 LI+V+D G I ERGTHQELL NGWYREQ++RQQ+ Sbjct: 541 LILVMDGGVIAERGTHQELLANNGWYREQYERQQL 575 >ZmdlA [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 590 Score = 427 bits (1098), Expect = e-119, Method: Compositional matrix adjust. Identities = 225/575 (39%), Positives = 326/575 (56%), Gaps = 1/575 (0%) Query: 7 NLGWFFRREKKRYXXXXXXXXXXXXXXXXPPRLLGNAIDDIVNGSITAGSLMKYIGLIVL 66 L W+FRRE +RY PP+++G +D + T G ++ +I +VL Sbjct: 6 QLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVDGVTEQHFTTGQILMWIATMVL 65 Query: 67 MLLIIYWITYIWMHKLFGGSNLVERLLRSRFMNHLMTMTPSFFERNRTGDLMARATNDIR 126 + +++Y + Y+W LFG S + LR + L P F+ R+RTGDLMARATND+ Sbjct: 66 IAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSRQHPEFYLRHRTGDLMARATNDVD 125 Query: 127 AVSATVGFGMLTLVDSTIFLTVVLLAMGFLVSWKLTXXXXXXXXXXXXXMIFYGKAIHDR 186 V G G+LTLVDS + VL+ M +SW+LT + G A+H+R Sbjct: 126 RVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQLTLFALLPMPVMAIMIKRNGDALHER 185 Query: 187 YSLAQDAFGDMNDQVLESVSGIRVIRAYVQERLDEKRFGEITEDVYRKNMAVAKVDSFFE 246 + LAQ AF +ND+ ES++ IR+I+A+ E F ED +KNM VA++D+ F+ Sbjct: 186 FKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDRQSALFAADAEDTGKKNMRVARIDARFD 245 Query: 247 PTIRLCVGLSYVIALTFGIYLVFRNQITLGDLVSFNMYLGMIVWPMFAIGELINIMQRGG 306 PTI + +G++ ++A+ G ++V + +TLG L SF MYLG+++WPM A+ + NI++RG Sbjct: 246 PTIYIAIGMANLLAIGGGSWMVVQGSLTLGQLTSFMMYLGLMIWPMLALAWMFNIVERGS 305 Query: 307 ASLERIDETLNSKPDVQDAALPVPVAQPTSIELKDVTFRYPTSTIDNLTGVSFTLTQGQT 366 A+ RI L P V D + PVP + +++ F YP + L V+F L GQ Sbjct: 306 AAYSRIRAMLAEAPVVNDGSEPVPEGR-GELDVNIHQFTYPQTDHPALENVNFALKPGQM 364 Query: 367 LGVVGRTGSGKSTLLKQLLHEYPTGRGEILISDVPIDKIALDQLHSWMGYVPQEQILFSK 426 LG+ G TGSGKSTLL + + G+I D+P+ K+ LD S + V Q LFS Sbjct: 365 LGICGPTGSGKSTLLSLIQRHFDVSEGDIRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSD 424 Query: 427 SVRENIQFGHHGASDERIMSAITAAAFQNXXXXXXXXXXXXVGERGVSLSGGQKQRVSIS 486 +V NI G A+ + I A+ + VGERGV LSGGQKQR+SI+ Sbjct: 425 TVANNIALGCPNATQQEIEHVARLASVHDDILRLPQGYDTEVGERGVMLSGGQKQRISIA 484 Query: 487 RAFIANPDILILDDALSAVDARTEARIIENIRDERAGKTTLISTHRLSAIEHADLIVVLD 546 RA + N +ILILDDALSAVD RTE +I+ N+R G+T +IS HRLSA+ A I+V+ Sbjct: 485 RALLVNAEILILDDALSAVDGRTEHQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQ 544 Query: 547 NGKIIERGTHQELLDMNGWYREQFDRQQVENNLTN 581 +G I +RG H L +GWYR+ + QQ+E L + Sbjct: 545 HGHIAQRGNHDVLAQQSGWYRDMYRYQQLEAALDD 579 >mdlA [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 590 Score = 427 bits (1098), Expect = e-119, Method: Compositional matrix adjust. Identities = 225/575 (39%), Positives = 326/575 (56%), Gaps = 1/575 (0%) Query: 7 NLGWFFRREKKRYXXXXXXXXXXXXXXXXPPRLLGNAIDDIVNGSITAGSLMKYIGLIVL 66 L W+FRRE +RY PP+++G +D + T G ++ +I +VL Sbjct: 6 QLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVDGVTEQHFTTGQILMWIATMVL 65 Query: 67 MLLIIYWITYIWMHKLFGGSNLVERLLRSRFMNHLMTMTPSFFERNRTGDLMARATNDIR 126 + +++Y + Y+W LFG S + LR + L P F+ R+RTGDLMARATND+ Sbjct: 66 IAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSRQHPEFYLRHRTGDLMARATNDVD 125 Query: 127 AVSATVGFGMLTLVDSTIFLTVVLLAMGFLVSWKLTXXXXXXXXXXXXXMIFYGKAIHDR 186 V G G+LTLVDS + VL+ M +SW+LT + G A+H+R Sbjct: 126 RVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQLTLFSLLPMPVMAIMIKRNGDALHER 185 Query: 187 YSLAQDAFGDMNDQVLESVSGIRVIRAYVQERLDEKRFGEITEDVYRKNMAVAKVDSFFE 246 + LAQ AF +ND+ ES++ IR+I+A+ E F ED +KNM VA++D+ F+ Sbjct: 186 FKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDRQSALFAADAEDTGKKNMRVARIDARFD 245 Query: 247 PTIRLCVGLSYVIALTFGIYLVFRNQITLGDLVSFNMYLGMIVWPMFAIGELINIMQRGG 306 PTI + +G++ ++A+ G ++V + +TLG L SF MYLG+++WPM A+ + NI++RG Sbjct: 246 PTIYIAIGMANLLAIGGGSWMVVQGSLTLGQLTSFMMYLGLMIWPMLALAWMFNIVERGS 305 Query: 307 ASLERIDETLNSKPDVQDAALPVPVAQPTSIELKDVTFRYPTSTIDNLTGVSFTLTQGQT 366 A+ RI L P V D + PVP + +++ F YP + L V+F L GQ Sbjct: 306 AAYSRIRAMLAEAPVVNDGSEPVPEGR-GELDVNIHQFTYPQTDHPALENVNFALKPGQM 364 Query: 367 LGVVGRTGSGKSTLLKQLLHEYPTGRGEILISDVPIDKIALDQLHSWMGYVPQEQILFSK 426 LG+ G TGSGKSTLL + + G+I D+P+ K+ LD S + V Q LFS Sbjct: 365 LGICGPTGSGKSTLLSLIQRHFDVSEGDIRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSD 424 Query: 427 SVRENIQFGHHGASDERIMSAITAAAFQNXXXXXXXXXXXXVGERGVSLSGGQKQRVSIS 486 +V NI G A+ + I A+ + VGERGV LSGGQKQR+SI+ Sbjct: 425 TVANNIALGCPNATQQEIEHVARLASVHDDILRLPQGYDTEVGERGVMLSGGQKQRISIA 484 Query: 487 RAFIANPDILILDDALSAVDARTEARIIENIRDERAGKTTLISTHRLSAIEHADLIVVLD 546 RA + N +ILILDDALSAVD RTE +I+ N+R G+T +IS HRLSA+ A I+V+ Sbjct: 485 RALLVNAEILILDDALSAVDGRTEHQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQ 544 Query: 547 NGKIIERGTHQELLDMNGWYREQFDRQQVENNLTN 581 +G I +RG H L +GWYR+ + QQ+E L + Sbjct: 545 HGHIAQRGNHDVLAQQSGWYRDMYRYQQLEAALDD 579 Score = 427 bits (1097), Expect = e-119, Method: Compositional matrix adjust. Identities = 225/575 (39%), Positives = 326/575 (56%), Gaps = 1/575 (0%) Query: 7 NLGWFFRREKKRYXXXXXXXXXXXXXXXXPPRLLGNAIDDIVNGSITAGSLMKYIGLIVL 66 L W+FRRE +RY PP+++G +D + T G ++ +I +VL Sbjct: 6 QLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVDGVTEQHFTTGQILMWIATMVL 65 Query: 67 MLLIIYWITYIWMHKLFGGSNLVERLLRSRFMNHLMTMTPSFFERNRTGDLMARATNDIR 126 + +++Y + Y+W LFG S + LR + L P F+ R+RTGDLMARATND+ Sbjct: 66 IAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSRQHPEFYLRHRTGDLMARATNDVD 125 Query: 127 AVSATVGFGMLTLVDSTIFLTVVLLAMGFLVSWKLTXXXXXXXXXXXXXMIFYGKAIHDR 186 V G G+LTLVDS + VL+ M +SW+LT + G A+H+R Sbjct: 126 RVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQLTLFALLPMPVMAIMIKRNGDALHER 185 Query: 187 YSLAQDAFGDMNDQVLESVSGIRVIRAYVQERLDEKRFGEITEDVYRKNMAVAKVDSFFE 246 + LAQ AF +ND+ ES++ IR+I+A+ E F ED +KNM VA++D+ F+ Sbjct: 186 FKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDRQSALFAADAEDTGKKNMRVARIDARFD 245 Query: 247 PTIRLCVGLSYVIALTFGIYLVFRNQITLGDLVSFNMYLGMIVWPMFAIGELINIMQRGG 306 PTI + +G++ ++A+ G ++V + +TLG L SF MYLG+++WPM A+ + NI++RG Sbjct: 246 PTIYIAIGMANLLAIGGGSWMVVQGSLTLGQLTSFMMYLGLMIWPMLALAWMFNIVERGS 305 Query: 307 ASLERIDETLNSKPDVQDAALPVPVAQPTSIELKDVTFRYPTSTIDNLTGVSFTLTQGQT 366 A+ RI L P V D + PVP + +++ F YP + L V+F L GQ Sbjct: 306 AAYSRIRAMLAEAPVVNDGSEPVPEGR-GELDVNIHQFTYPQTDHPALENVNFALKPGQM 364 Query: 367 LGVVGRTGSGKSTLLKQLLHEYPTGRGEILISDVPIDKIALDQLHSWMGYVPQEQILFSK 426 LG+ G TGSGKSTLL + + G+I D+P+ K+ LD S + V Q LFS Sbjct: 365 LGICGPTGSGKSTLLSLIQRHFDVSEGDIRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSD 424 Query: 427 SVRENIQFGHHGASDERIMSAITAAAFQNXXXXXXXXXXXXVGERGVSLSGGQKQRVSIS 486 +V NI G A+ + I A+ + VGERGV LSGGQKQR+SI+ Sbjct: 425 TVANNIALGCPNATQQEIEHVARLASVHDDILRLPQGYDTEVGERGVMLSGGQKQRISIA 484 Query: 487 RAFIANPDILILDDALSAVDARTEARIIENIRDERAGKTTLISTHRLSAIEHADLIVVLD 546 RA + N +ILILDDALSAVD RTE +I+ N+R G+T +IS HRLSA+ A I+V+ Sbjct: 485 RALLVNAEILILDDALSAVDGRTEHQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQ 544 Query: 547 NGKIIERGTHQELLDMNGWYREQFDRQQVENNLTN 581 +G I +RG H L +GWYR+ + QQ+E L + Sbjct: 545 HGHIAQRGNHDVLAQQSGWYRDMYRYQQLEAALDD 579 >ECs0502 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 590 Score = 427 bits (1097), Expect = e-119, Method: Compositional matrix adjust. Identities = 225/575 (39%), Positives = 326/575 (56%), Gaps = 1/575 (0%) Query: 7 NLGWFFRREKKRYXXXXXXXXXXXXXXXXPPRLLGNAIDDIVNGSITAGSLMKYIGLIVL 66 L W+FRRE +RY PP+++G +D + T G ++ +I +VL Sbjct: 6 QLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVDGVTEQHFTTGQILMWIATMVL 65 Query: 67 MLLIIYWITYIWMHKLFGGSNLVERLLRSRFMNHLMTMTPSFFERNRTGDLMARATNDIR 126 + +++Y + Y+W LFG S + LR + L P F+ R+RTGDLMARATND+ Sbjct: 66 IAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSRQHPEFYLRHRTGDLMARATNDVD 125 Query: 127 AVSATVGFGMLTLVDSTIFLTVVLLAMGFLVSWKLTXXXXXXXXXXXXXMIFYGKAIHDR 186 V G G+LTLVDS + VL+ M +SW+LT + G A+H+R Sbjct: 126 RVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQLTLFALLPMPVMAIMIKRNGDALHER 185 Query: 187 YSLAQDAFGDMNDQVLESVSGIRVIRAYVQERLDEKRFGEITEDVYRKNMAVAKVDSFFE 246 + LAQ AF +ND+ ES++ IR+I+A+ E F ED +KNM VA++D+ F+ Sbjct: 186 FKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDRQSALFAADAEDTGKKNMRVARIDARFD 245 Query: 247 PTIRLCVGLSYVIALTFGIYLVFRNQITLGDLVSFNMYLGMIVWPMFAIGELINIMQRGG 306 PTI + +G++ ++A+ G ++V + +TLG L SF MYLG+++WPM A+ + NI++RG Sbjct: 246 PTIYIAIGMANLLAIGGGSWMVVQGSLTLGQLTSFMMYLGLMIWPMLALAWMFNIVERGS 305 Query: 307 ASLERIDETLNSKPDVQDAALPVPVAQPTSIELKDVTFRYPTSTIDNLTGVSFTLTQGQT 366 A+ RI L P V D + PVP + +++ F YP + L V+F L GQ Sbjct: 306 AAYSRIRAMLAEAPVVNDGSEPVPEGR-GELDVNIHQFTYPQTDHPALENVNFALKPGQM 364 Query: 367 LGVVGRTGSGKSTLLKQLLHEYPTGRGEILISDVPIDKIALDQLHSWMGYVPQEQILFSK 426 LG+ G TGSGKSTLL + + G+I D+P+ K+ LD S + V Q LFS Sbjct: 365 LGICGPTGSGKSTLLSLIQRHFDVSEGDIRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSD 424 Query: 427 SVRENIQFGHHGASDERIMSAITAAAFQNXXXXXXXXXXXXVGERGVSLSGGQKQRVSIS 486 +V NI G A+ + I A+ + VGERGV LSGGQKQR+SI+ Sbjct: 425 TVANNIALGCPNATQQEIEHVARLASVHDDILRLPQGYDTEVGERGVMLSGGQKQRISIA 484 Query: 487 RAFIANPDILILDDALSAVDARTEARIIENIRDERAGKTTLISTHRLSAIEHADLIVVLD 546 RA + N +ILILDDALSAVD RTE +I+ N+R G+T +IS HRLSA+ A I+V+ Sbjct: 485 RALLVNAEILILDDALSAVDGRTEHQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQ 544 Query: 547 NGKIIERGTHQELLDMNGWYREQFDRQQVENNLTN 581 +G I +RG H L +GWYR+ + QQ+E L + Sbjct: 545 HGHIAQRGNHDVLAQQSGWYRDMYRYQQLEAALDD 579 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.139 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,872,900 Number of extensions: 1467280 Number of successful extensions: 21272 Number of sequences better than 1.0e-05: 1698 Number of HSP's gapped: 19670 Number of HSP's successfully gapped: 1797 Length of query: 582 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 470 Effective length of database: 25,461,023 Effective search space: 11966680810 Effective search space used: 11966680810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 119 (50.4 bits)