BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0021.p (313 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ygaD [V] COG1132 ABC-type multidrug transport system, ATPase ... 340 2e-93 BH0941 [V] COG1132 ABC-type multidrug transport system, ATPase a... 325 5e-89 SA1683 [V] COG1132 ABC-type multidrug transport system, ATPase a... 311 1e-84 BS_yknU [V] COG1132 ABC-type multidrug transport system, ATPase ... 112 9e-25 lin0155 [V] COG1132 ABC-type multidrug transport system, ATPase ... 104 2e-22 >BS_ygaD [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 165 Score = 340 bits (872), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 0/290 (0%), Positives = 0/290 (0%), Gaps = 1/290 (0%) Query: 2 KWIVLTLFIGMIKFSIPLTLPLILKYVVDVLLNNPVLSVTERVSKLMMVLGGSLVLFVII 61 + K + V +N + + V ++ Sbjct: 25 TTAESCTGGWVAKVITDIAGSSAWFERGFVTYSNEA-KAQMIGVREETLAQHGAVSEPVV 83 Query: 62 RGPVEYYRQYFAQLITSRVLFDMRNRLYGHLQRLSLRYYQNTKVGEAISRFINDVEQSKN 121 + + + + ++ ++ Sbjct: 84 VEMAIGALKAARADYAVSISGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRD 143 Query: 122 LVEVGMMNVWLDMFTLVFALAFMFYLNPVLALVSIAILPLYGIAVNTLYKRLKLLTKDRS 181 Sbjct: 144 203 Query: 182 QALAAIQGYLHERIQGIAIIRSFTMERVDKKQFKDINGKFLEKAMAQTRWNAFTFAIINT 241 Sbjct: 204 263 Query: 242 LTDIAPLLVIGYGGYQVIHGNLSLGTFVAFFGYLDRMYAPLRRLINSSTV 291 Sbjct: 264 313 >BH0941 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 584 Score = 325 bits (834), Expect = 5e-89, Method: Compositional matrix adjust. Identities = 149/289 (51%), Positives = 214/289 (74%) Query: 2 KWIVLTLFIGMIKFSIPLTLPLILKYVVDVLLNNPVLSVTERVSKLMMVLGGSLVLFVII 61 K I +T+ IG++KF IPL +PL++ Y++D ++ ++ E+ S L V+GG +F+++ Sbjct: 19 KQIFITIIIGLLKFGIPLLIPLLVAYIIDGIIFAEQMTNEEKFSALFTVMGGIFFIFIVL 78 Query: 62 RGPVEYYRQYFAQLITSRVLFDMRNRLYGHLQRLSLRYYQNTKVGEAISRFINDVEQSKN 121 R P+EYYRQYFAQ I +++L+D+R+ L+ H+Q+LSLR+Y N KVGE ISR I+DVEQ+K Sbjct: 79 RPPIEYYRQYFAQWIGNKILYDIRDHLFTHIQKLSLRFYSNRKVGEIISRVIHDVEQTKT 138 Query: 122 LVEVGMMNVWLDMFTLVFALAFMFYLNPVLALVSIAILPLYGIAVNTLYKRLKLLTKDRS 181 V G+MN+WLDMFT++ ALA MF +N L LV+IA+ P YG ++ Y +L+ LT+ RS Sbjct: 139 FVMTGLMNIWLDMFTIIIALAIMFSMNAWLTLVAIAMFPFYGFSIKYFYAKLRHLTRVRS 198 Query: 182 QALAAIQGYLHERIQGIAIIRSFTMERVDKKQFKDINGKFLEKAMAQTRWNAFTFAIINT 241 QALA +QG+LHER+QG+++IRSF +E ++ QF NG FL +A+ T+WNA TFA++NT Sbjct: 199 QALADVQGHLHERVQGMSVIRSFALEDYEQTQFAKRNGTFLNRAIDHTKWNAKTFAVVNT 258 Query: 242 LTDIAPLLVIGYGGYQVIHGNLSLGTFVAFFGYLDRMYAPLRRLINSST 290 +TDIAPLLVI VI+G L +G AF Y++R+Y PLRRL+NSST Sbjct: 259 VTDIAPLLVIFVAACFVINGELEVGAMTAFVLYMERLYNPLRRLVNSST 307 >SA1683 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 578 Score = 311 bits (796), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 138/287 (48%), Positives = 208/287 (72%) Query: 4 IVLTLFIGMIKFSIPLTLPLILKYVVDVLLNNPVLSVTERVSKLMMVLGGSLVLFVIIRG 63 I T+ +G+IKF IP+ +PL++KY +D ++NN L+ E+V L + +G +L +FVI+R Sbjct: 16 IFATIIVGIIKFGIPMLIPLLIKYAIDGVINNHALTTDEKVHHLTIAIGIALFIFVIVRP 75 Query: 64 PVEYYRQYFAQLITSRVLFDMRNRLYGHLQRLSLRYYQNTKVGEAISRFINDVEQSKNLV 123 P+E+ RQY AQ ++++L+D+R +LY HLQ LS R+Y N +VG+ ISR INDVEQ+K+ + Sbjct: 76 PIEFIRQYLAQWTSNKILYDIRKKLYNHLQALSARFYANNQVGQVISRVINDVEQTKDFI 135 Query: 124 EVGMMNVWLDMFTLVFALAFMFYLNPVLALVSIAILPLYGIAVNTLYKRLKLLTKDRSQA 183 G+MN+WLD T++ AL+ MF+L+ L L ++ I P Y + V + RL+ LT++RSQA Sbjct: 136 LTGLMNIWLDCITIIIALSIMFFLDVKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQA 195 Query: 184 LAAIQGYLHERIQGIAIIRSFTMERVDKKQFKDINGKFLEKAMAQTRWNAFTFAIINTLT 243 LA +QG+LHER+QGI++++SF +E + K F N FL +A+ TRWNA++FA INT+T Sbjct: 196 LAEVQGFLHERVQGISVVKSFAIEDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVT 255 Query: 244 DIAPLLVIGYGGYQVIHGNLSLGTFVAFFGYLDRMYAPLRRLINSST 290 DI P++VIG G Y I G++++GT AF GYL+ ++ PLRRL+ S T Sbjct: 256 DIGPIIVIGVGAYLAISGSITVGTLAAFVGYLELLFGPLRRLVASFT 302 >BS_yknU [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 585 Score = 112 bits (280), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 74/284 (26%), Positives = 139/284 (48%), Gaps = 12/284 (4%) Query: 3 WIVLTLFIGMIKFSIPLTLPLILKYVVDVLLNNPVLSVTERVSKLMMVLGGSLVLFVIIR 62 W +L + ++ +I + P+IL+ +D + V R +L + + ++++ Sbjct: 20 WSLLAM---LLMTAITVVYPIILQITIDEI-------VLGRQYQLAAWVSLGFIAVMVLK 69 Query: 63 GPVEYYRQYFAQLITSRVLFDMRNRLYGHLQRLSLRYYQNTKVGEAISRFINDVEQSKNL 122 G ++ QY + + ++ +RN LY LQRLS YY N K G+ +SR DVE + Sbjct: 70 GMATFFHQYLGDMFGIKSVYRLRNGLYEKLQRLSFSYYDNAKTGDLMSRLTADVEGLRFF 129 Query: 123 VEVGMMNVWLDMFTLVFALAFMFYLNPVLALVSIAILPLYGIAVNTLYKRLKLLTKDRSQ 182 + G+ + + +L+ MFY + L LV+IA+LP +AV KR+ + + Sbjct: 130 LSYGLAELIRFGLLVAISLSVMFYYSVPLTLVTIAVLPFLAVAVYRFDKRVHPAFRGIRK 189 Query: 183 ALAAIQGYLHERIQGIAIIRSFTMERVDKKQFKDINGKFLEKAMAQTR-WNAFTFAIINT 241 + A + + E I GI ++S + E F N ++ + + + W+A+ F ++ Sbjct: 190 SFAKLNTKVQENISGINTVKSLSREDFQISTFNKANAEYRAQYLQTSSIWSAY-FPLMEF 248 Query: 242 LTDIAPLLVIGYGGYQVIHGNLSLGTFVAFFGYLDRMYAPLRRL 285 + + + ++ YGGY V+ L+ G VAFF ++ M P+ L Sbjct: 249 IGNTCIVALLSYGGYLVMQNQLNPGELVAFFSLVNYMMWPIMNL 292 >lin0155 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 593 Score = 104 bits (259), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 71/283 (25%), Positives = 141/283 (49%), Gaps = 12/283 (4%) Query: 2 KWIVLTLFIGMIKFSIPLTLPLILKYVVDVLLNNPVLSVTERVSKLMMVLGGSLVLFVII 61 K I +TLF+ ++ + P + K V+D + N ++ ++ + +V VI+ Sbjct: 33 KSIYITLFVILLANVATMIGPYLTKVVIDETIPNKNMTQLFWIAVIFIV-------SVIV 85 Query: 62 RGPVEYYRQYFAQLITSRVLFDMRNRLYGHLQRLSLRYYQNTKVGEAISRFINDVEQSKN 121 G YR LI +L DMR ++GHLQ+L Y+ + G+ + R +N + + Sbjct: 86 TGLCMRYRIRSITLIGQDILKDMRTAIFGHLQKLPFSYFDSRPHGKILIRVVNYINMLSD 145 Query: 122 LVEVGMMNVWLDMFTLVFALAFMFYLNPVLALVSIAILPLYGIAVNTLYKRLKLLTKDRS 181 L+ G++N+ D+ +++ L FM ++PVL L S+A++P+ + V + + + S Sbjct: 146 LLSNGLINLISDILSVIVTLGFMLMIDPVLTLYSLALIPVLFVIVMVIKTAQRKAYQVLS 205 Query: 182 QALAAIQGYLHERIQGIAIIRSFTMERVDKKQFKDINGKFLEKAMAQTRWNAFTFAIINT 241 + + Y+HE I GI + +SF+ E + + F +++ ++ M + + + Sbjct: 206 NKQSNMNAYIHESIAGIKVTQSFSREEENFEIFTEVSNEYRRSWMKAVKIQFLLWPGVQN 265 Query: 242 LTDIAPLLV--IGYGGYQVIHGNLSLGTFVAFFGYLDRMYAPL 282 + + L+ +G GY V ++S GT +AF GY+ + P+ Sbjct: 266 IAVMTTCLIYFVGIKGYGV---DVSTGTLIAFIGYVGNFWNPV 305 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.144 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,875,305 Number of extensions: 825800 Number of successful extensions: 2901 Number of sequences better than 1.0e-05: 147 Number of HSP's gapped: 2818 Number of HSP's successfully gapped: 150 Length of query: 313 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 207 Effective length of database: 26,153,027 Effective search space: 5413676589 Effective search space used: 5413676589 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 116 (49.3 bits)