BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0016.p (328 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1623 [O] COG0492 Thioredoxin reductase 308 7e-84 BS_ypdA [O] COG0492 Thioredoxin reductase 301 7e-82 SA1311 [O] COG0492 Thioredoxin reductase 292 4e-79 DR2623 [O] COG0492 Thioredoxin reductase 249 3e-66 L00196 [O] COG0492 Thioredoxin reductase 69 9e-12 >BH1623 [O] COG0492 Thioredoxin reductase Length = 346 Score = 308 bits (790), Expect = 7e-84, Method: Compositional matrix adjust. Identities = 18/326 (5%), Positives = 45/326 (13%), Gaps = 7/326 (2%) Query: 1 MKDVIIIGAGPCGLSAAIECQRRGLSSLIIEKNFIVHSIYLYPTNMQFFSTTALLEIGDV 60 + L S Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 61 PFTSPNDKPFRHEALVYYRRAAEQHGLDIAAYEEALSVKPLEDGTFAVHTRNKRGETYTR 120 T + + + + + + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALH-----DS 117 Query: 121 TAANVIISTGYFDQPNLIGIPGEELPKVTHYFGEAHPYTGMKVTVIGGSNSAVDAALELL 180 + + A + + + Sbjct: 118 LKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIG 177 Query: 181 RVGAEVDMVYRGASISENIKPWVRPIFESMVAKDKIVLHLESRVTEITPGSVHITGRNGE 240 V R +++ ++ + + G + Sbjct: 178 VSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYS-CL 236 Query: 241 VKELDNDFVLALTGFRPSRTLLSSAGVSMDETQEKPAFNPSTMESNVPGLYIAGVIASGR 300 L +R L S Q + A + GL A + + Sbjct: 237 GFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFV 296 Query: 301 NANEVFIETGRGHGKLIADHIISKRL 326 T G + + Sbjct: 297 RRQPFVASTLLGATTMDQL-KTNIES 321 >BS_ypdA [O] COG0492 Thioredoxin reductase Length = 346 Score = 301 bits (772), Expect = 7e-82, Method: Compositional matrix adjust. Identities = 17/320 (5%), Positives = 41/320 (12%), Gaps = 5/320 (1%) Query: 5 IIIGAGPCGLSAAIECQRRGLSSLIIEKNFIVHSIYLYPTNMQFFSTTALLEIGDVPFTS 64 L S T Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66 Query: 65 PNDKPFRHEALVYYRRAAEQHGLDIAAYEEALSVKPLEDGTFAVHTRNKRGETYTRTAAN 124 + + + + + + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREAL---HDSL--KRL 121 Query: 125 VIISTGYFDQPNLIGIPGEELPKVTHYFGEAHPYTGMKVTVIGGSNSAVDAALELLRVGA 184 + + A + + + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 185 EVDMVYRGASISENIKPWVRPIFESMVAKDKIVLHLESRVTEITPGSVHITGRNGEVKEL 244 V R +++ ++ + + G + L Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 245 DNDFVLALTGFRPSRTLLSSAGVSMDETQEKPAFNPSTMESNVPGLYIAGVIASGRNANE 304 ++ TL S E +K + +A R Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPF 301 Query: 305 VFIETGRGHGKLIADHIISK 324 V I Sbjct: 302 VASTLLGATTMDQLKTNIES 321 >SA1311 [O] COG0492 Thioredoxin reductase Length = 346 Score = 292 bits (748), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 18/323 (5%), Positives = 43/323 (13%), Gaps = 9/323 (2%) Query: 5 IIIGAGPCGLSAAIECQRRGLSSLIIEKNFIVHSIYLYPTNMQFFSTTALLEIGDVPFTS 64 L S T Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66 Query: 65 PNDKPFRHEALVYYRRAAEQHGLDIAAYEEALSVKPLEDGTFAVHTRNKRGETYTRTAAN 124 + + + + + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQ-ALDRKNIREALHDSLKRLQ--- 122 Query: 125 VIISTGYFDQPNLIGIPGEELPKVTHYFGEAHPYTGMKVTVIGGSNSAVDAALELLRVGA 184 Q + P K+ + + ++ P + T+ + + + V Sbjct: 123 --TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 185 EVDMVYRGASISENIKPWVRPIFESMVAKDKIVLHLESRVTEITPGSVHITGRNGEVKEL 244 E + R + V + + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELL-AYSCLGFG 239 Query: 245 DNDFVLALTGFRPSRTLLSSAGVSM--DETQEKPAFNPSTMESNVPGLYIAGVIASGRNA 302 + + E +K + +A R Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 303 NEVFIETGRGHGKLIADHIISKR 325 V I Sbjct: 300 PFVASTLLGATTMDQLKTNIESL 322 >DR2623 [O] COG0492 Thioredoxin reductase Length = 346 Score = 249 bits (637), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 12/324 (3%), Positives = 46/324 (14%), Gaps = 7/324 (2%) Query: 1 MKDVIIIGAGPCGLSAAIECQRRGLSSLIIEKNFIVHSIYLYPTNMQFFSTTALLEIGDV 60 + L S Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 61 PFTSPNDKPFRHEALVYYRRAAEQHGLDIAAYEEALSVKPLEDGTFAVHTRNKRGETYTR 120 T + + + + + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALD-RKNIREALHDSLKRL 121 Query: 121 TAANVIISTGYFDQPNLIGIPGEELPKVTHYFGEAHPYTGMKVTVIGGSNSAVDAALELL 180 + + ++ Q + T + + + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 181 RVGAEVDMVYRGASISENIKPWVRPIFESMVAKDKIVLHLESRVTEITPGSVHITGRNGE 240 + ++ ++ + + ++ L S+ + + G Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 241 VKELDNDFVLALTGFRPSRTLLSSAGVSMDETQEKPAFNPSTMESNVPGLYIAGVIASGR 300 + N A + A+ + +A + Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTR--YSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 301 NANEVFIETGRGHGKLIADHIISK 324 + + Sbjct: 300 PFVASTLLGATTM----DQLKTNI 319 >L00196 [O] COG0492 Thioredoxin reductase Length = 346 Score = 68.9 bits (167), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 14/300 (4%), Positives = 46/300 (15%), Gaps = 36/300 (12%) Query: 3 DVIIIGAGPCGLSAAIECQRRGLSSLIIEKNFIV--HSIYLYPTNMQFFSTTALLEIGDV 60 + L S + Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEM-------YPVPP 57 Query: 61 PFTSPNDKPFRHEALVYYRRAAEQHGLDIAAYEEALSVKPLEDGTFAVHTRNKRGETYTR 120 + V A + A V Sbjct: 58 RPETQGLTETY----VGNWLAKHGSREKLI---IASKVSGPSRNNDKGIR-----PDQAL 105 Query: 121 TAANVIISTGY-FDQPNLIGIPGEEL----PKVTHYFGEAHPYTGMKVTVIGGSNSAVDA 175 N+ + + + ++ + + +T V A Sbjct: 106 DRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALA 165 Query: 176 ALELLRVGAEVDMVYRGASISENIKPWVRPIFESMVAKDKIVLHLESRVTEITPGSVHIT 235 + + + A + + ++ +++ + + Sbjct: 166 EYQRAGKIRYIGVSNETAFGVMR------YLHLADKHDLPRIVTIQNPYSLLNRSFEVGL 219 Query: 236 G-RNGEVKELDNDFVLALTGFRPSRTLLSSAGVSMDETQEKPAFNPSTMESNVPGLYIAG 294 + + G + L + + + +A Sbjct: 220 AEVSQYEGVELLAYSCLGFGTLTGKYLNGA---KPAGARNTLFSRFTRYSGEQTQKAVAA 276 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,668,504 Number of extensions: 1005893 Number of successful extensions: 11088 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 11096 Number of HSP's successfully gapped: 28 Length of query: 328 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 221 Effective length of database: 26,037,693 Effective search space: 5754330153 Effective search space used: 5754330153 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)