BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0006.p (335 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0620 [P] COG0715 ABC-type nitrate/sulfonate/bicarbonate trans... 276 3e-74 BS_ytlA [P] COG0715 ABC-type nitrate/sulfonate/bicarbonate trans... 244 2e-64 BS_ytlB [P] COG0715 ABC-type nitrate/sulfonate/bicarbonate trans... 98 2e-20 mll6979 [P] COG0715 ABC-type nitrate/sulfonate/bicarbonate trans... 88 2e-17 AGl1197 [P] COG0715 ABC-type nitrate/sulfonate/bicarbonate trans... 86 1e-16 >CAC0620 [P] COG0715 ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components Length = 338 Score = 276 bits (706), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 132/323 (40%), Positives = 200/323 (61%), Gaps = 5/323 (1%) Query: 17 CLSVLAGCG--SDSAAVKVKIGEVTRSVFYAPEYVALSQNFFKDEGLEAELQTIPGGDKT 74 C++ GC ++S+ V++ EVTRS+FYAP YVA+ +FK+ G+ +L T G DKT Sbjct: 17 CIT-FTGCSKPTNSSLTTVRLNEVTRSIFYAPMYVAIKNGYFKENGINLDLSTGEGADKT 75 Query: 75 MTALLSGAIDVALVGSETSIYVYQQGAEDPVINFAQLTQRDGTFLFARKADADFNWDKLK 134 M +LS + D+ G E IY+Y + +D I FA+LTQ+DG+FL R + F W LK Sbjct: 76 MQQVLSKSADIGFCGPEQVIYIYNKHRKDYPIVFAKLTQKDGSFLVGRHENKSFTWKNLK 135 Query: 135 GSTFLGQRKGGMPQMAGAFTLLQKGIDAGKDLTLIQNIDFANIAGAFASGTGDYVQLFEP 194 G T +G R GG+P+MA + L G+ GKD+ +I NI+F+++ GAF GTGDY LFEP Sbjct: 136 GKTVIGGRPGGVPEMAFEYALKSNGLKPGKDVNIITNINFSSVGGAFKGGTGDYATLFEP 195 Query: 195 TASIFEREHRGSVVASFGVESGYLPYTVFMSKQSYISKNGDTVQKFTNAIQRAQVWVKEH 254 T S+ E+ G + AS G +GYLPYT F + +SYI+KN +Q FTNAI + Q+W+ +H Sbjct: 196 TGSLLEQSKAGYINASVGTAAGYLPYTCFFTTKSYINKNPKVIQGFTNAIYKGQLWMSKH 255 Query: 255 SPEEIADAILPYFENTDRDIVISSVKRYKEQDTYAVDPVIDGKEWNNLLDVMDNAGE--L 312 S +E A I +F + ++ +S+K YK D YA +P++ +E N +D++ + + + Sbjct: 256 SDKETASLIKSFFPGSSTTLIQNSIKNYKSIDAYASNPLLKEEEMNRFMDIIQSYDKDLI 315 Query: 313 KARVPEAKIVDNSFAEKAEASVK 335 K R I + FA++A ++K Sbjct: 316 KTRPAFNTITNEKFAKEAIKNIK 338 >BS_ytlA [P] COG0715 ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components Length = 229 Score = 244 bits (622), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 120/194 (61%), Positives = 142/194 (73%) Query: 1 MNLRKKISLPLMILTMCLSVLAGCGSDSAAVKVKIGEVTRSVFYAPEYVALSQNFFKDEG 60 MN ++ + + LA C + KVK+ EVT S+FYAP YVA S+ FFK+EG Sbjct: 1 MNRWLRLGFACVGSIFLMFALAACKQEETLTKVKVAEVTHSIFYAPLYVAESKGFFKEEG 60 Query: 61 LEAELQTIPGGDKTMTALLSGAIDVALVGSETSIYVYQQGAEDPVINFAQLTQRDGTFLF 120 L+ ++ T GGDKTMT+LLS D+ALVGSETSIYV QGA+DPVINFAQLTQ DGTFL Sbjct: 61 LDVDVNTTWGGDKTMTSLLSNGSDIALVGSETSIYVEAQGAKDPVINFAQLTQTDGTFLV 120 Query: 121 ARKADADFNWDKLKGSTFLGQRKGGMPQMAGAFTLLQKGIDAGKDLTLIQNIDFANIAGA 180 AR+ F+WD+LKG TFLGQRKGGMPQM G + L + ID KD+ LIQNIDFANIA A Sbjct: 121 AREDVEHFDWDQLKGKTFLGQRKGGMPQMVGEYVLNKHKIDPHKDIDLIQNIDFANIANA 180 Query: 181 FASGTGDYVQLFEP 194 FASGTG+YVQLFEP Sbjct: 181 FASGTGEYVQLFEP 194 >BS_ytlB [P] COG0715 ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components Length = 105 Score = 97.8 bits (242), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 45/100 (45%), Positives = 69/100 (69%) Query: 230 ISKNGDTVQKFTNAIQRAQVWVKEHSPEEIADAILPYFENTDRDIVISSVKRYKEQDTYA 289 + KN DT KFT AI +AQ WV+ HS ++I DAI F++TD D++ +S++RYK+Q +Y+ Sbjct: 1 MKKNEDTAVKFTKAIYKAQQWVENHSAKDITDAIKDEFDDTDPDVIETSIERYKKQHSYS 60 Query: 290 VDPVIDGKEWNNLLDVMDNAGELKARVPEAKIVDNSFAEK 329 DP+++ KEW L +MD +GEL +P ++V+ AEK Sbjct: 61 PDPLLNEKEWELLQTIMDKSGELPKHIPYNQLVNKEIAEK 100 >mll6979 [P] COG0715 ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components Length = 363 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 73/291 (25%), Positives = 135/291 (46%), Gaps = 17/291 (5%) Query: 4 RKKISLPLMILTMCLSVLAGCGSDSAAVKVKIGEVTRSVFYAPEYVALSQNFFKDEGLEA 63 R++I + ++L + +V + + V++ +G + P VA ++ EGLE Sbjct: 26 RRRIPMRKLMLALAATVAFAGSAFAEPVRISVGSYNLNNLPFP--VAQGLGLYEKEGLEV 83 Query: 64 ELQTI-PGGDKTMTALLSGAIDVALVGSETSIYVYQQGAEDPVINFAQLTQRDGTFLFAR 122 ++ GG KT+ AL++G+ D+A+ + +I + Q + V+ F L + G + A Sbjct: 84 TVENFASGGSKTLQALVAGSTDIAVGFYDHTIQM--QAQKKAVVAFVMLARNSG-LVLAG 140 Query: 123 KADADF---NWDKLKGSTFLGQRKGGMPQMAGAFTLLQKGIDAGKDLTLIQNIDFANIAG 179 + + F N + +KG+ G + L + G+ A D++LI + Sbjct: 141 GSKSTFDPANPETIKGAKIGITSPGSSSDFFVRYYLQRHGLSA-DDISLIGVGSGSAAVA 199 Query: 180 AFASGTGDYVQLFEPTASIFEREHRGSVV-------ASFGVESGYLPYTVFMSKQSYISK 232 A G D + ++P A+ E + G ++ + + G P +V + QS+I Sbjct: 200 ALEQGKVDLLVNYDPAATFIEAKGVGKILIDGRSDEGAKAIYGGIYPTSVLYATQSFIEA 259 Query: 233 NGDTVQKFTNAIQRAQVWVKEHSPEEIADAILPYFENTDRDIVISSVKRYK 283 N T+QK TNA +A W+ HS EEI + + F + DRD + +V+ K Sbjct: 260 NPQTIQKVTNATVKALEWMNSHSAEEIVEKLPKEFISGDRDTYVKAVENAK 310 >AGl1197 [P] COG0715 ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components Length = 134 Score = 85.5 bits (210), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 0/250 (0%), Positives = 0/250 (0%), Gaps = 17/250 (6%) Query: 46 PEYVALSQNFFKDEGLEAELQTIP-GGDKTMTALLSGAIDVALVGSETSIYVYQQGAEDP 104 Q K + + + + + ++ + + + Sbjct: 36 AVSYKSFQELPKAKAPAVLQKLAQAVAAEGFSGIQINKALSSIDAHQETSGSGRIQTL-- 93 Query: 105 VINFAQLTQRDGTFLFARKADADFNWDKLKGSTFLGQRKG-GMPQMAGAFTL---LQKGI 160 + Q A K G Sbjct: 94 RVVARQKGAAVR-IDAVFNIQAGQ--IADKDVIRKGICDII 150 Query: 161 DAGKDLTLIQNIDFANIAGAFASGTGDYVQLFEPTASIFEREHRGSVV-------ASFGV 213 Sbjct: 151 210 Query: 214 ESGYLPYTVFMSKQSYISKNGDTVQKFTNAIQRAQVWVKEHSPEEIADAILPYFENTDRD 273 Sbjct: 211 270 Query: 274 IVISSVKRYK 283 Sbjct: 271 280 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,220,919 Number of extensions: 1093719 Number of successful extensions: 2011 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 2013 Number of HSP's successfully gapped: 13 Length of query: 335 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 228 Effective length of database: 26,037,693 Effective search space: 5936594004 Effective search space used: 5936594004 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)