BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0005.p (259 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ytlC [P] COG1116 ABC-type nitrate/sulfonate/bicarbonate trans... 270 2e-72 CAC0619 [P] COG1116 ABC-type nitrate/sulfonate/bicarbonate trans... 243 2e-64 PA3514 [P] COG1116 ABC-type nitrate/sulfonate/bicarbonate transp... 203 2e-52 RSc1199 [P] COG1116 ABC-type nitrate/sulfonate/bicarbonate trans... 192 4e-49 PA2294 [P] COG1116 ABC-type nitrate/sulfonate/bicarbonate transp... 189 3e-48 >BS_ytlC [P] COG1116 ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component Length = 260 Score = 270 bits (689), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 128/250 (51%), Positives = 166/250 (66%) Query: 2 LPIVQLKEVTHAYLGDREASLAVENFSLYVEAGEFVSLVGPSGCGKTTLLSIIAGLLQPS 61 + + + VTH Y +E + AV + E G+F+S +GPSGCGKTTLLSIIAGL++PS Sbjct: 1 MSFLHVDHVTHTYFSIKEKTTAVRDIHFDAEKGDFISFLGPSGCGKTTLLSIIAGLIEPS 60 Query: 62 RGEVFVSGHTVSGPSPEVGYMLQQDYLFPWRTILDNALLGLELTGRLNESSRQKTIGLLG 121 G V + G + +GYMLQQDYLFPW++I +N LLGL++ L E S+ +GLL Sbjct: 61 EGRVLIEGREPNQKEHNIGYMLQQDYLFPWKSIEENVLLGLKIADTLTEESKAAALGLLP 120 Query: 122 EMGLAGKEHAYPAQLSGGMRQRVALVRTLSTDPGLLLLDEPFSALDYQIKLQLEDLVSET 181 E GL E YP +LSGGMRQR AL RTL+ +P LLLLDEPFSALD+Q KL LE+LV T Sbjct: 121 EFGLIDVEKKYPKELSGGMRQRAALARTLAPNPSLLLLDEPFSALDFQTKLSLENLVFRT 180 Query: 182 LRRRGTTAILVTHDLSEAIAVSSRVILLQPNPGKIRKIFTVPEAIRSTPPLYARDLPGFA 241 L+ TA+LVTHD+ EAIA+S + L PG I +IFT+P+ + + P AR P F Sbjct: 181 LKEYQKTAVLVTHDIGEAIAMSDTIFLFSNQPGTIHQIFTIPKELAAMLPFDARQEPSFQ 240 Query: 242 ELFHEVWKEM 251 LF +WKE+ Sbjct: 241 TLFQTIWKEL 250 >CAC0619 [P] COG1116 ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component Length = 253 Score = 243 bits (621), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 118/249 (47%), Positives = 167/249 (67%), Gaps = 2/249 (0%) Query: 5 VQLKEVTHAYLGDREASLAVENFSLYVEAGEFVSLVGPSGCGKTTLLSIIAGLLQPSRGE 64 V +K V+ Y + A++N S V G+FVS+VGPSGCGK+TLL+IIAGLL+PS GE Sbjct: 4 VYIKNVSVNYHSVSAETKALKNISFKVPDGDFVSIVGPSGCGKSTLLNIIAGLLKPSSGE 63 Query: 65 VFVSGHTVSGPSPEVGYMLQQDYLFPWRTILDNALLGLELTGRLNESSRQKTIGLLGEMG 124 VF+ V G SP++GYM Q+D LF W ++ DN LGL++ LN+ ++QK LL + Sbjct: 64 VFIDNILVKGVSPKIGYMFQKDNLFQWLSVWDNVALGLKIKKELNDKTKQKIEELLEKYD 123 Query: 125 LAGKEHAYPAQLSGGMRQRVALVRTLSTDPGLLLLDEPFSALDYQIKLQLEDLVSETLRR 184 L + +P +LSGGMRQRVAL+RTL DP +LLLDEPFSALDYQ +L + + E ++ Sbjct: 124 LKDFKDHHPNELSGGMRQRVALIRTLVLDPEVLLLDEPFSALDYQTRLNVGSEICEIIKT 183 Query: 185 RGTTAILVTHDLSEAIAVSSRVILLQPNPGKIRKIFTVPEAIRSTPPLYARDLPGFAELF 244 TAI+VTHD++EA+++S RVI+L P ++K V + P+ R+ P F+ F Sbjct: 184 ECKTAIMVTHDIAEAVSISKRVIVLSKRPATLKK--DVKITLPDVSPIKRRECPDFSLYF 241 Query: 245 HEVWKEMEL 253 ++VWKEM++ Sbjct: 242 NDVWKEMDI 250 >PA3514 [P] COG1116 ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component Length = 283 Score = 203 bits (517), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 114/244 (46%), Positives = 149/244 (61%), Gaps = 1/244 (0%) Query: 16 GDREASLAVENFSLYVEAGEFVSLVGPSGCGKTTLLSIIAGLLQPSRGEVFVSGHTVSGP 75 G A+LAV+ L + GE VS++GPSGCGK+TLL++ AGL PS GEV V G VSGP Sbjct: 40 GKSAATLAVQGLDLAIRRGEVVSIIGPSGCGKSTLLNMGAGLYAPSEGEVRVGGERVSGP 99 Query: 76 SPEVGYMLQQDYLFPWRTILDNALLGLELTGRLNESSRQKTIGLLGEMGLAGKEHAYPAQ 135 +V +MLQ+D L PWR+I N LGLE+ GR R +L + LAG YP Q Sbjct: 100 VRKVSFMLQKDLLMPWRSIRRNIELGLEIDGRAAGERRAIAEEMLEKCHLAGFAEHYPFQ 159 Query: 136 LSGGMRQRVALVRTLSTDPGLLLLDEPFSALDYQIKLQLEDLVSETLRRRGTTAILVTHD 195 LSGGMRQR AL RTL+TDP +L LDEPFSALD Q K+ L+ ++ L + TA+ +THD Sbjct: 160 LSGGMRQRAALARTLATDPQVLFLDEPFSALDAQTKMILQQDLARMLCEQRKTALFITHD 219 Query: 196 LSEAIAVSSRVILLQPNPGKIRKIFTVPEAIRSTPPLYARDLPGFAELFHEVWKEMELAG 255 L EAIA+S R++++ PG I + V +R PL R LP L + +++ Sbjct: 220 LVEAIAMSDRILVMSARPGTIVEEIEVGLPLRDN-PLERRKLPEIGPLVGRLMTLLKVGE 278 Query: 256 RGEL 259 EL Sbjct: 279 SAEL 282 >RSc1199 [P] COG1116 ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component Length = 270 Score = 192 bits (488), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 110/251 (43%), Positives = 152/251 (60%), Gaps = 7/251 (2%) Query: 3 PIVQLKEVTHAYL-GDREAS--LAVENFSLYVEAGEFVSLVGPSGCGKTTLLSIIAGLLQ 59 P + +T ++ D+ A AV++ SL V+AGEFVS+VGP+GCGK+TLL++ AGLLQ Sbjct: 4 PALSFDRITCTFISADQPAQRYTAVQDTSLDVQAGEFVSVVGPTGCGKSTLLNLAAGLLQ 63 Query: 60 PSRGEVFVSGHTVSGPSPEVGYMLQQDYLFPWRTILDNALLGLELTGRLNESSRQKTIGL 119 PS G+V V G ++G + GYM Q + L PWR+ L N L GLE G ++ Sbjct: 64 PSSGQVRVFGEPLAGINRRAGYMFQAEALMPWRSALGNVLAGLEFRGMPAAEAQDLARSW 123 Query: 120 LGEMGLAGKEHAYPAQLSGGMRQRVALVRTLSTDPGLLLLDEPFSALDYQIKLQLEDLVS 179 L +GLAG E YP QLSGGMR+RV+L +TL DP ++L+DEPFSALD Q + +E+ V Sbjct: 124 LARVGLAGFEDRYPHQLSGGMRKRVSLAQTLVLDPDIILMDEPFSALDIQTRQLMENEVL 183 Query: 180 ETLRRRGTTAILVTHDLSEAIAVSSRVILLQPNPGKIRKI--FTVPEAIRSTPPLYARDL 237 + + + +THDL EAIA+S RV++L PG R I F + + R RD Sbjct: 184 DLWAAKRKAVLFITHDLDEAIAMSDRVVVLAAGPGT-RPIGEFAI-DLPRPRDVAEIRDD 241 Query: 238 PGFAELFHEVW 248 GF +L +W Sbjct: 242 AGFVDLHARIW 252 >PA2294 [P] COG1116 ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component Length = 284 Score = 189 bits (480), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 94/212 (44%), Positives = 136/212 (64%) Query: 5 VQLKEVTHAYLGDREASLAVENFSLYVEAGEFVSLVGPSGCGKTTLLSIIAGLLQPSRGE 64 +Q+++++ EA AV + +L GEFV L+GPSGCGK+TLL +AG L+PS G Sbjct: 11 IQIRDLSIRLGQGSEAFEAVRDLNLEARPGEFVCLLGPSGCGKSTLLGALAGHLRPSAGS 70 Query: 65 VFVSGHTVSGPSPEVGYMLQQDYLFPWRTILDNALLGLELTGRLNESSRQKTIGLLGEMG 124 V V G +V GPSP+ G + QQ LFPWR + DN GL++ G ++ + +LG +G Sbjct: 71 VRVDGRSVEGPSPQRGMVFQQHTLFPWRRVRDNVAFGLKMQGLARAERNRRAMEMLGLVG 130 Query: 125 LAGKEHAYPAQLSGGMRQRVALVRTLSTDPGLLLLDEPFSALDYQIKLQLEDLVSETLRR 184 LA +P QLSGGM+QRV + R L P LLL+DEPF ALD Q +L+++ L+ + R Sbjct: 131 LADFAGRWPGQLSGGMQQRVEIARVLVNRPRLLLMDEPFGALDAQTRLKMQTLLLDVWAR 190 Query: 185 RGTTAILVTHDLSEAIAVSSRVILLQPNPGKI 216 TT + VTHD+ EA+ ++ RV+++ P PG+I Sbjct: 191 VRTTVLFVTHDIDEALYLADRVLVMSPRPGRI 222 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,099,680 Number of extensions: 752705 Number of successful extensions: 18591 Number of sequences better than 1.0e-05: 2176 Number of HSP's gapped: 15997 Number of HSP's successfully gapped: 2408 Length of query: 259 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 155 Effective length of database: 26,383,695 Effective search space: 4089472725 Effective search space used: 4089472725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)