BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0004.p (201 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ytlD [P] COG0600 ABC-type nitrate/sulfonate/bicarbonate trans... 183 2e-46 CAC0618 [P] COG0600 ABC-type nitrate/sulfonate/bicarbonate trans... 164 1e-40 RSc1200 [P] COG0600 ABC-type nitrate/sulfonate/bicarbonate trans... 98 8e-21 AGc1933 [P] COG0600 ABC-type nitrate/sulfonate/bicarbonate trans... 86 3e-17 AGl1202 [P] COG0600 ABC-type nitrate/sulfonate/bicarbonate trans... 83 3e-16 >BS_ytlD [P] COG0600 ABC-type nitrate/sulfonate/bicarbonate transport system, permease component Length = 270 Score = 183 bits (464), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 97/195 (49%), Positives = 121/195 (62%) Query: 1 MASGSLWPHLGMXXXXXXXXXXXXXXXXXXXAIVIWWSPFLSAVLDPYMVVFNSMPKVAL 60 + GSL H+G+ A ++WWS L+ +LDPY+V+ N+MPKVAL Sbjct: 69 LGDGSLLSHIGVTLFETVLGFLLGTFMGTCLAALLWWSNRLARILDPYLVILNAMPKVAL 128 Query: 61 GPIFIVMFGAGFTAXXXXXXXXXXXXXXXXXYNSFCSVDPNLVKVARSFGASRVQVFNKV 120 GPI IV G F + Y +F VD N KV ++FGA + +F +V Sbjct: 129 GPILIVALGPSFISIIAMGAIISVIITTIVVYTAFQEVDENYTKVMKTFGAKKWVIFKEV 188 Query: 121 ILPSSFPTVVSTLKVNVGMSWVGVIVGEFLAAKSGLGYLIIYGFQVFNFTLVMSSLLIIA 180 ILP+S P ++STLKVNVG+SWVGVIVGEFL +K GLGY+IIYGFQVFNFTLV SLLIIA Sbjct: 189 ILPASSPAIISTLKVNVGLSWVGVIVGEFLVSKVGLGYMIIYGFQVFNFTLVFLSLLIIA 248 Query: 181 AVATAMYQLVVYVEK 195 AT MYQ V +EK Sbjct: 249 IFATLMYQGVELLEK 263 >CAC0618 [P] COG0600 ABC-type nitrate/sulfonate/bicarbonate transport system, permease component Length = 264 Score = 164 bits (414), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 82/199 (41%), Positives = 116/199 (58%) Query: 2 ASGSLWPHLGMXXXXXXXXXXXXXXXXXXXAIVIWWSPFLSAVLDPYMVVFNSMPKVALG 61 G+L+ H+G+ AI++WWS F S VLDPY+VV NS+PK+AL Sbjct: 65 GEGALFTHIGITCFETVVGFLLSTILGTFIAILLWWSDFASKVLDPYLVVLNSLPKIALA 124 Query: 62 PIFIVMFGAGFTAXXXXXXXXXXXXXXXXXYNSFCSVDPNLVKVARSFGASRVQVFNKVI 121 PI I G G A NSF V+ +K+ ++FGA++ Q+ +I Sbjct: 125 PIIIFWMGNGIKAIIVITVLISIVVTIITVLNSFKEVEDEKIKLMKTFGANKFQILKHLI 184 Query: 122 LPSSFPTVVSTLKVNVGMSWVGVIVGEFLAAKSGLGYLIIYGFQVFNFTLVMSSLLIIAA 181 +PSS PT++S LK+NVG+SWVGVI+GEFL AK GLG+LI+YG Q+ +VM S++I++ Sbjct: 185 IPSSIPTLMSALKINVGLSWVGVIMGEFLVAKEGLGFLIVYGGQISELDMVMMSIIILSI 244 Query: 182 VATAMYQLVVYVEKLILSR 200 +A MY V EK + R Sbjct: 245 LAYLMYVAVAIFEKRLQKR 263 >RSc1200 [P] COG0600 ABC-type nitrate/sulfonate/bicarbonate transport system, permease component Length = 263 Score = 98.2 bits (243), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 63/196 (32%), Positives = 94/196 (47%) Query: 3 SGSLWPHLGMXXXXXXXXXXXXXXXXXXXAIVIWWSPFLSAVLDPYMVVFNSMPKVALGP 62 G ++ HLG+ + + SP SAVLDPY+ NSMP+V L P Sbjct: 56 EGDIYQHLGVTLAETVLAFGIGTATGLGAGLWLALSPSASAVLDPYIKAANSMPRVILAP 115 Query: 63 IFIVMFGAGFTAXXXXXXXXXXXXXXXXXYNSFCSVDPNLVKVARSFGASRVQVFNKVIL 122 IF V FG G + Y V P ++ AR GASR Q+ V L Sbjct: 116 IFGVWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGASRRQLLRFVYL 175 Query: 123 PSSFPTVVSTLKVNVGMSWVGVIVGEFLAAKSGLGYLIIYGFQVFNFTLVMSSLLIIAAV 182 PS+ V S+L +VG+++VG +VGE+L + G+GYLI+ F+ V + ++++ Sbjct: 176 PSATSWVFSSLHTSVGLAFVGAVVGEYLGSARGVGYLILQAEGTFDINTVFAGIVVLTVF 235 Query: 183 ATAMYQLVVYVEKLIL 198 A + LV EK ++ Sbjct: 236 ALVLDWLVGLAEKRLM 251 >AGc1933 [P] COG0600 ABC-type nitrate/sulfonate/bicarbonate transport system, permease component Length = 816 Score = 86.3 bits (212), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 7/159 (4%), Positives = 25/159 (15%) Query: 37 WSPFLSAVLDPYMVVFNSMPKVALGPIFIVMFGAGFTAXXXXXXXXXXXXXXXXXYNSFC 96 + V+ + + + + + Sbjct: 101 EGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSVWLKLRIRRNGKLHEIGF 160 Query: 97 SVDPNLVKVARSFGASRVQVFNKVILPSSFPTVVSTLKVNVGMSWVGVIVGEFLAAKSGL 156 + ++ L S ++ + + + L Sbjct: 161 THGVADAPLSVIGEYEGRSGTEVTFLASPQTFTMTDYDYGTLEHRLRELAFLNSGVRILL 220 Query: 157 GYLIIYGFQVFNFTLVMSSLLIIAAVATAMYQLVVYVEK 195 + + + A LV Sbjct: 221 TDKRHSDVKQQELLYDGGLEAFVRYLDRAKKPLVDKPIA 259 >AGl1202 [P] COG0600 ABC-type nitrate/sulfonate/bicarbonate transport system, permease component Length = 134 Score = 82.8 bits (203), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 0/164 (0%), Positives = 0/164 (0%) Query: 38 SPFLSAVLDPYMVVFNSMPKVALGPIFIVMFGAGFTAXXXXXXXXXXXXXXXXXYNSFCS 97 Sbjct: 93 152 Query: 98 VDPNLVKVARSFGASRVQVFNKVILPSSFPTVVSTLKVNVGMSWVGVIVGEFLAAKSGLG 157 Sbjct: 153 212 Query: 158 YLIIYGFQVFNFTLVMSSLLIIAAVATAMYQLVVYVEKLILSRR 201 Sbjct: 213 256 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.140 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,212,275 Number of extensions: 351880 Number of successful extensions: 1616 Number of sequences better than 1.0e-05: 81 Number of HSP's gapped: 1595 Number of HSP's successfully gapped: 81 Length of query: 201 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 100 Effective length of database: 26,729,697 Effective search space: 2672969700 Effective search space used: 2672969700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 113 (48.1 bits)