BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0449.0011.p (273 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy0902 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 157 2e-38 L169390 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 157 2e-38 DR0235 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 144 2e-34 mlr4825 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 86 5e-17 CC1323 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 73 4e-13 >SPy0902 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 484 Score = 157 bits (397), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 91/249 (36%), Positives = 140/249 (56%), Gaps = 4/249 (1%) Query: 1 MLDLLQTLQPEAAFQTPLYPGVYRDDLMKPLQRKLRVAYTTASPVGTPVSGEAVAAVLKT 60 +L LQ Q E+ F PL + +D + + LQR L +A+ G+PVS + A+ + Sbjct: 220 LLYYLQMEQMESPF--PL-ATLTKDSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQA 276 Query: 61 VKWLQAEGHE-VEEKLSPVNGVWLMENYYTMNAGEVAAMFMSLENDMGRSIAAHEVDIVT 119 V WL+ +GH+ VE + PVN ++ +YY MN+ E AAMF +E+ GR + +++ +T Sbjct: 277 VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMETMT 336 Query: 120 WVLGEAGKKVTAAEFVHSLNEWDVAAAQMAGLLSRYDLYITPANADSAPEIGELTQQPEE 179 W + ++GK + A + L +WD +A MA YDL +T AP+ GEL + Sbjct: 337 WAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKL 396 Query: 180 IEALLHISELPKEAQQRMIYEMFEPSLTYTPFTQLANLTGQPAMSVPLHLTPAGLPIGVQ 239 + L E Q ++ MF SL P+T L NLTGQPA+S+P + T GL +G+Q Sbjct: 397 MANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEGLSMGIQ 456 Query: 240 VMASKGRED 248 ++A+KGRED Sbjct: 457 LIAAKGRED 465 >L169390 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 499 Score = 157 bits (397), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 89/254 (35%), Positives = 148/254 (58%), Gaps = 11/254 (4%) Query: 5 LQTLQPEAAFQTPLYPGVYRDDLMKP--------LQRKLRVAYTTASPV-GTPVSGEAVA 55 +QTLQ A +Q L L K L RK+++AY+ SP+ G +S +A+ Sbjct: 223 MQTLQEAAPYQAKLLDEKELIALTKSTGQAVSNNLSRKIKIAYSLQSPISGIELSKDAIN 282 Query: 56 AVLKTVKWLQAEGHEVEEKLSPVNGVWLMENYYTMNAGEVAAMFMSLENDMGRSIAAHEV 115 A LK V++L+ +GHE+ E P+ L+++YY MNA E+ AM E GR + + +V Sbjct: 283 ACLKAVEFLERQGHELTEVDFPIEARPLIQSYYRMNAAEIVAMLEPWERAAGRKLTSQDV 342 Query: 116 DIVTWVLGEAGKKVTAAEFVHSLNEWDVAAAQMAGLLSR-YDLYITPANADSAPEIGELT 174 +++T+ L EAG+K + ++++L +WD+AA + + YDL++T A +AP+IG+ Sbjct: 343 ELLTYALLEAGRKAPVSSYINALAQWDLAARDFTDKIYKDYDLFLTATTAKTAPKIGQEL 402 Query: 175 QQPEEIEALLHISELPKEAQQRMIYEMFEPSLTYTPFTQLANLTGQPAMSVPLHLT-PAG 233 E ++ + IS+ E Q +I FE SL +TP+ ++NLTGQPA+S+P+++ Sbjct: 403 MTAEVLKEMSEISQYEFEQQIEIIEAAFERSLAFTPYNFISNLTGQPALSLPVYVNEETN 462 Query: 234 LPIGVQVMASKGRE 247 LP+G+Q+ K E Sbjct: 463 LPLGIQLWGPKNSE 476 >DR0235 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 561 Score = 144 bits (362), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 99/255 (38%), Positives = 139/255 (54%), Gaps = 12/255 (4%) Query: 1 MLDLLQTLQPEAAFQTPLYPGVYRDDLMKPLQRKLRVAYTTASPVGTPVSGEAVAAVLKT 60 +LDL Q AA P Y +++ + R LR+ ++TA P+G V E VAAV Sbjct: 276 LLDLEQGPDAGAALFLPSPERPYSEEVGREPGR-LRIGFSTAHPLGRSVHPECVAAVQGA 334 Query: 61 VKWLQAEGHEVEEKLSPVNGVWLMENYYTMNAGEVAAMFMSLENDMGRSIAAHEVDIVTW 120 + L++ GHEVEE P +G L + + + GE A +L + +GR A +V+ VTW Sbjct: 335 ARLLESLGHEVEEVALPWDGPALAQAFLMLYFGETGASLAALRDTLGRPARASDVEAVTW 394 Query: 121 VLGEAGKKVTAAEFVHSLNEWDVAAAQMAGLLSRYDLYITPANADSAPEIGELTQQPEEI 180 +LG+ G+ +AA+F + W+V A M YDL +TP A +IGEL QP + Sbjct: 395 LLGQLGRSYSAADFAAARASWNVHARAMGRFHQNYDLLLTPVLATPPLQIGEL--QPRGV 452 Query: 181 EALL-------HISELPKEAQQRMIYEMFEPSLTYTPFTQLANLTGQPAMSVPLHLTPAG 233 +A L +S L + + Q + + L P+TQLANLTGQPAMSVPLH T G Sbjct: 453 QAALLRAAQQMDVSGLLRRSGQ--VDALATDILEKMPYTQLANLTGQPAMSVPLHWTADG 510 Query: 234 LPIGVQVMASKGRED 248 LP+GVQ +A RED Sbjct: 511 LPVGVQFVAPLARED 525 >mlr4825 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 301 Score = 85.9 bits (211), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 0/243 (0%), Positives = 0/243 (0%), Gaps = 4/243 (1%) Query: 34 KLRVAYTTASPVGTPVSGEAVAAVLKTVKWLQAEGHEVEEKLSPVNGVWLMENYYTMNAG 93 Sbjct: 256 315 Query: 94 EVAAMFMSLENDMGRSIAAHEVDIVTWVLGEAGKKVTAAEFVHSLNEWDVAAAQMAGLLS 153 Sbjct: 316 - 374 Query: 154 RYDLYITPANADSAPEIGELTQQ--PEEIEALL-HISELPKEAQQRMIYEMFEPSLTYTP 210 Sbjct: 375 434 Query: 211 FTQLANLTGQPAMSVPLHLTPAGLPIGVQVMASKGREDXXXXXXXXXXXXXXWVGMKGNP 270 Sbjct: 435 494 Query: 271 LFP 273 Sbjct: 495 497 >CC1323 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 521 Score = 73.2 bits (178), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 62/220 (28%), Positives = 100/220 (45%), Gaps = 23/220 (10%) Query: 30 PLQRKLRVAYTTASPVGTPVSGEAVAAVLKTVKWLQAEGHEVEEKLSPVNGVWLMENYYT 89 PL+RKL+V + G E AAV K L+ GH VE P++G +++ Sbjct: 298 PLKRKLKVGLVMNTGTGLTPHPEVQAAVNNAAKLLKDLGHRVEPTNWPMDGNQFGQDFTV 357 Query: 90 MNAGEVAAMFMSLENDMGRSIAAHEVDIVTWVLGEAGKKVTAAEFVHSLNEWDVAAA--Q 147 + A A + + MGR A+ ++ + + AE V++L + + AA + Sbjct: 358 LWASGAAELVAGIGKAMGRKPDANVLEPFSLGM---------AELVNTLPQGALQAAMER 408 Query: 148 MAGLLSRYDLYITPANADSAPEIGELTQQPEEIEALLHISELPKEAQQRMIYEMFEPSLT 207 + + YD ++T + +P +G+ + + + EL Q R+ L Sbjct: 409 LNATIKAYDTWLTKYDVILSPVLGKPPVELGYVAGWVPFEEL----QARL--------LA 456 Query: 208 YTPFTQLANLTGQPAMSVPLHLTPAGLPIGVQVMASKGRE 247 Y +T + N+ G PAMSVPLH T GLP+GVQ G E Sbjct: 457 YVGYTPVQNVAGAPAMSVPLHWTADGLPVGVQFAGRAGDE 496 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.131 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,123,344 Number of extensions: 807451 Number of successful extensions: 3887 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 3881 Number of HSP's successfully gapped: 10 Length of query: 273 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 168 Effective length of database: 26,268,361 Effective search space: 4413084648 Effective search space used: 4413084648 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)