BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0449.0001.p (419 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_tyrS [J] COG0162 Tyrosyl-tRNA synthetase 491 e-139 VC0465 [J] COG0162 Tyrosyl-tRNA synthetase 371 e-102 CAC0637 [J] COG0162 Tyrosyl-tRNA synthetase 312 8e-85 YPL097w [J] COG0162 Tyrosyl-tRNA synthetase 285 7e-77 >BS_tyrS [J] COG0162 Tyrosyl-tRNA synthetase Length = 346 Score = 491 bits (1264), Expect = e-139, Method: Compositional matrix adjust. Identities = 0/398 (0%), Positives = 0/398 (0%), Gaps = 2/398 (0%) Query: 2 NIIDELEWRDAINQQTDAEGLRELTNTKSVSLYCGVDPTGDSMHIGHLIPFMVLKRFQLA 61 + E + I + V R + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 62 GHRPVILIGGATGTIGDPSGRQSERSLQTLEQVQANVDALTAQMKKLFVTES-DNQVRMV 120 G + + + + A +K D+ R+ Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 121 NNYDWTHKINVIEFLRDYGKNFSINTMLAKDVVSSRLDSGISFTEFSYQILQSLDYLHLY 180 +Y ++++ + + + + VS ++ + + Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 181 QHEDVQLQVGGSDQWGNITSGLDLIRKKEGADAKVFGLTIPLMLKSDGTKFGKSAGGAIW 240 ++ + +V + + + G + Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 241 LDPKKTTPYEFYQFWANSDDRDVVKYLKYFTFLTREEIEALAEKVQTEPHKREAQKALAE 300 + S +I + ++ Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFV 302 Query: 301 EMTRFVHGEELLEQAKRITAALFSGDIRSLTAEEIEEGFKEMPTY-TSGKESKNIVDWLV 359 Sbjct: 303 362 Query: 360 DLGIEPSKRQAREDITKGAISLNGERVTELGTEVTAED 397 Sbjct: 363 400 Score = 394 bits (1012), Expect = e-109, Method: Compositional matrix adjust. Identities = 0/419 (0%), Positives = 0/419 (0%), Gaps = 6/419 (1%) Query: 2 NIIDELEWRDAINQQTDAEGLRELTNTKSVSLYCGVDPTGDSMHIGHLIPFMVLKRFQLA 61 I + T G + L V + + + + P Q Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGL 64 Query: 62 GHRPVILIGGATGTIGDPSGRQSERSLQTLEQVQANVDALTAQMKKLFVTES-DNQVRMV 120 V G+ D + +++ Sbjct: 65 TETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD 124 Query: 121 NNYDWTHKINVIEFLRDYGKNFSINTMLAKDVVSSRL---DSGISFTEFSYQILQSLDYL 177 + +S + L + Y + + Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 178 HLYQHEDVQLQVGGSDQWGNITSGLDLIRKKEGADAKVFGLTIPLMLKSDGTKFGKSAGG 237 + ++ + + L R E + + G Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPYSLLNRSFE--VGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 238 AIWLDPKKTTPYEFYQFWANSDDRDVVKYLKYFTFLTREEIEALAEKVQTEPHKREAQKA 297 +L+ K F + ++ L R Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFV 302 Query: 298 LAEEMTRFVHGEELLEQAKRITAALFSGDIRSLTAEEIEEGFKEMPTYTSGKESKNIVDW 357 Sbjct: 303 362 Query: 358 LVDLGIEPSKRQAREDITKGAISLNGERVTELGTEVTAEDAXXXXXXXXXXXXXNYSLV 416 Sbjct: 363 421 >VC0465 [J] COG0162 Tyrosyl-tRNA synthetase Length = 346 Score = 371 bits (953), Expect = e-102, Method: Compositional matrix adjust. Identities = 0/393 (0%), Positives = 0/393 (0%), Gaps = 8/393 (2%) Query: 3 IIDELEWRDAINQQTDAEGLRELTNTKSVSLYCGVDPTGDSMHIGHLIPFMVLKRFQLAG 62 L Q ++A+ +L + +D + + + Sbjct: 14 STLGLGTMTFGEQNSEADAHAQLDYAVAQ-GINLIDVAEMYPVPPRPETQGLTETYVGNW 72 Query: 63 HRPVILIGGATGTIGDPSGRQSERSLQTLEQVQANVDALTAQMKKLFVTESDNQVRM--V 120 ++ +Q + A L ++D Sbjct: 73 LAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVH 132 Query: 121 NNYDWTHKINVIEFLRDYGKNFSINTMLAKDVVSSRL---DSGISFTEFSYQILQSLDYL 177 T+ + + + + I + + + +L Sbjct: 133 WPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHL 192 Query: 178 HLYQHEDVQLQVGGSDQWGNITSGLDLIRKKEGADAKVFGLTIPLMLKSDGTKFGKSAGG 237 + + N + + L + V L + T + Sbjct: 193 ADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEG--VELLAYSCLGFGTLTGKYLNGAK 250 Query: 238 AIWLDPKKTTPYEFYQFWANSDDRDVVKYLKYFTFLTREEIEALAEKVQTEPHKREAQKA 297 + + Y + L ++ + Q Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGAT 310 Query: 298 LAEEMTRFVHGEELLEQAKRITAALFSGDIRSLTAEEIEEGFKEMPTYTSGKESKNIVDW 357 Sbjct: 311 370 Query: 358 LVDLGIEPSKRQAREDITKGAISLNGERVTELG 390 Sbjct: 371 403 >CAC0637 [J] COG0162 Tyrosyl-tRNA synthetase Length = 346 Score = 312 bits (799), Expect = 8e-85, Method: Compositional matrix adjust. Identities = 0/398 (0%), Positives = 0/398 (0%), Gaps = 17/398 (4%) Query: 2 NIIDELEWRDAINQQTDAEGLRELTNTKSVSLYCGVDPTGDSMHIGHLIPFMVLKRFQLA 61 + E + I + V R + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 62 GHRPVILIGGATGTIGDPSGRQSERSLQTLEQVQANVDALTAQMKKLFVTESDNQVRMVN 121 G + + + + A +K + E+ + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRK-NIREALHDSLKRL 121 Query: 122 NYDWTHKINVIEFLRDYGKNFSINTMLAKDVVSSRLDSGISFTEFSYQILQSLDYLHLYQ 181 D+ V R + + L + + + + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 182 HEDVQLQVGGSDQWGNITSGLDLIRKKEGADAKVFGLTIPLMLKSDGTKFGKSAGGAIWL 241 ++ ++ + + + + + + Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPY--SLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 242 DPKKTTPYEFYQFWANSDDRDVVKYLKYFTFLTREEIEALAEKVQTEPHKREAQKALAEE 301 A + + Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPA--QMALAFVR 297 Query: 302 MTRFVHGEELLEQAKRITAALFSGDIRSLTAEEIEEGFKEMPTYTSGKE--SKNIVDWLV 359 FV L + Sbjct: 298 RQPFVASTLLGATTMDQLKTNIESLH----------LELSEDVLAEIEAVH 347 Query: 360 DLGIEPSKRQAREDITKGAISLNGERVTELGTEVTAED 397 Sbjct: 348 385 >YPL097w [J] COG0162 Tyrosyl-tRNA synthetase Length = 346 Score = 285 bits (730), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 0/401 (0%), Positives = 0/401 (0%), Gaps = 21/401 (5%) Query: 2 NIIDELEWRDAINQQTDAEG-LR-ELTNTKSVSLYCGVDPTGDSMHIGHLIPFMVLKRFQ 59 + + + + + ++ Sbjct: 55 VPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREAL 114 Query: 60 LAGHRPVILIGGATGTIGDPSGRQSERSLQTLEQVQANVDALTAQMKKLF---------- 109 + + + P + + Sbjct: 115 HDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIR 174 Query: 110 --VTE--SDNQVRMVNNYDWTHKINVIEFLRDYGKNFSINTMLAKDVVSSRLDS--GISF 163 + V + H + I +++ + + + VS Sbjct: 175 YIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYS 234 Query: 164 TEFSYQILQSLDYLHLYQHEDVQLQVGGSDQWGNITSGLDLIRKKEGADAK--VFGLTIP 221 + L + G T + + Sbjct: 235 CLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALA 294 Query: 222 LMLKSDGTKFGKSAGGAIWLDPKKTTPYEFYQFWANSDDRDVVKYLKYFTFLTREEIEAL 281 Sbjct: 295 354 Query: 282 AEKVQTEPHKREAQKALAEEMTRFVHGEELLEQAKRITAALFSGDIRSLTAEEIEEGFKE 341 Sbjct: 355 414 Query: 342 MPTYTSGKESKNIVDWLVDLGIEPSKRQAREDITKGAISLN 382 Sbjct: 415 - 454 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,999,854 Number of extensions: 1254201 Number of successful extensions: 3166 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 3130 Number of HSP's successfully gapped: 13 Length of query: 419 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 310 Effective length of database: 25,807,025 Effective search space: 8000177750 Effective search space used: 8000177750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)