BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0010.p (224 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ybcL [G] COG2814 Arabinose efflux permease 218 6e-57 BS_ydhL [G] COG2814 Arabinose efflux permease 79 7e-15 SMb21168 [G] COG2814 Arabinose efflux permease 64 1e-10 mll2510 [G] COG2814 Arabinose efflux permease 62 8e-10 ydhP [G] COG2814 Arabinose efflux permease 56 4e-08 >BS_ybcL [G] COG2814 Arabinose efflux permease Length = 183 Score = 218 bits (555), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 0/211 (0%), Positives = 0/211 (0%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 Sbjct: 168 227 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXX 120 Sbjct: 228 287 Query: 121 XXXXXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQSXXXXXXXXXXXXXX 180 Sbjct: 288 347 Query: 181 XXXXXXXLSSITWIGAASVFLATSATLILYR 211 Sbjct: 348 378 >BS_ydhL [G] COG2814 Arabinose efflux permease Length = 125 Score = 78.6 bits (192), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 0/166 (0%), Positives = 0/166 (0%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 Sbjct: 203 262 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXX 120 Sbjct: 263 322 Query: 121 XXXXXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQS 166 Sbjct: 323 368 >SMb21168 [G] COG2814 Arabinose efflux permease Length = 394 Score = 64.3 bits (155), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 48/172 (27%), Positives = 74/172 (43%), Gaps = 6/172 (3%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 LGL +++L +PR + +S++ + P VA L VTF +L G + +YL+P Sbjct: 166 LGLTCAAVLWLRLPRGLSGTKLTMSERFGAIGRPGVASSLMVTFLYLTGGFMIISYLAPL 225 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXX 120 I +G++ + G LL FG+ ++IG+ G+ ADR G + Sbjct: 226 AIDGAGLSQLALPGLLLAFGVGAVIGNLSSGYLADRLGATRVVTASLISALAVSLMIAFG 285 Query: 121 XXXXXXXXAVLLL------WSFAAWSTGPTQQYHITTLKPEASGVMLGLNQS 166 A LLL W W+ P Q I PE + + L LN S Sbjct: 286 LHFLTRDLAGLLLIGIMVPWGIVGWAFPPAQASRIVGFAPEVAHLTLSLNAS 337 >mll2510 [G] COG2814 Arabinose efflux permease Length = 1091 Score = 61.6 bits (148), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 10/172 (5%), Positives = 19/172 (11%), Gaps = 6/172 (3%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 L + Q + L + G + Sbjct: 166 TVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKG 225 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPT------LFGXXXXXXXXX 114 + + + + G V T Sbjct: 226 WQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRAEGTANTT 285 Query: 115 XXXXXXXXXXXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQS 166 L + V G Sbjct: 286 VVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAG 337 >ydhP [G] COG2814 Arabinose efflux permease Length = 389 Score = 56.2 bits (134), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 43/164 (26%), Positives = 74/164 (45%), Gaps = 1/164 (0%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 LG+ISM+ +F ++P+ + ++LA+L P+V L T G YTY+SP Sbjct: 168 LGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMFTLYTYISPV 227 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXX 120 L +I+ +++ L++ G+ IG+ +GG ADR V TL G Sbjct: 228 LQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADR-SVNGTLKGFLLLLMVIMLAIPFL 286 Query: 121 XXXXXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLN 164 +++W A ++ P Q + + EA G+ +N Sbjct: 287 ARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVN 330 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.139 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,559,236 Number of extensions: 433463 Number of successful extensions: 944 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 938 Number of HSP's successfully gapped: 32 Length of query: 224 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 121 Effective length of database: 26,499,029 Effective search space: 3206382509 Effective search space used: 3206382509 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)