BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0009.p (254 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3178 [R] COG2220 Predicted Zn-dependent hydrolases of the beta... 221 7e-58 AF1265 [R] COG2220 Predicted Zn-dependent hydrolases of the beta... 187 2e-47 TM1162 [R] COG2220 Predicted Zn-dependent hydrolases of the beta... 176 2e-44 PAB0339 [R] COG2220 Predicted Zn-dependent hydrolases of the bet... 176 4e-44 MA1308 [R] COG2220 Predicted Zn-dependent hydrolases of the beta... 172 4e-43 >BH3178 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Length = 252 Score = 221 bits (564), Expect = 7e-58, Method: Compositional matrix adjust. Identities = 11/229 (4%), Positives = 31/229 (13%), Gaps = 3/229 (1%) Query: 26 MKIIYHGHSCIQIEVSGKSLIIDPFLRGNPAAVTKPEEVKTDMVLLTHAHVDHILDAAPI 85 M + + + Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 86 ALQNNVPIVANVELAGYMEGQGVQTIGMNIGGTVDLGFAKATMIHAFHSSGITLEDGQKA 145 + I + +DL FA A++ + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELF-PHLVSL 119 Query: 146 IYGGMPAGFIIQAEGRTVVHTGDTGLFGDLKMFGELYDIDLAILPIGGHYTMGPEHALIA 205 + + H + + + + + I Sbjct: 120 LNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIW 179 Query: 206 AKWLGAKQVLPIHYNTFPPIVQDANAFVRALGEEGIRGTVLGYGESLEL 254 + + E + L + Sbjct: 180 RTTYYHQMPSHQAIIDWVTATGLRPWLQDLT--ESEQQLFLKRYHQMLE 226 >AF1265 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Length = 252 Score = 187 bits (474), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 13/231 (5%), Positives = 40/231 (17%), Gaps = 4/231 (1%) Query: 26 MKIIYHGHSCIQIEVSGKSLIIDPFLRGNPAAVTKPEEVKTDMVLLTHAHVDHILDAAPI 85 M + + + R V ++ T A Sbjct: 1 MSDWNPSLYLHFSAERSRPAV-ELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARIT 59 Query: 86 ALQNNVPIVANVELAGYMEGQGVQTIGMNIGGTVDLGFAKATMIHAFHSSGITLEDGQKA 145 + ++ ++A A I + + Sbjct: 60 GIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLV-S 118 Query: 146 IYGGMPAGFIIQAEGRTVVHTGDTGLFGDLKMFGELYDIDLAILPIGGHYTMGPEHALIA 205 + + + + + + LA + + Sbjct: 119 LLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDI 178 Query: 206 AKWLGAKQVLPIHYNTFPPIVQDANAFVRALGEEG--IRGTVLGYGESLEL 254 + Q+ +++ L E + + Sbjct: 179 WRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQY 229 >TM1162 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Length = 252 Score = 176 bits (447), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 13/230 (5%), Positives = 38/230 (16%), Gaps = 5/230 (2%) Query: 26 MKIIYHGHSCIQIEVSGKSLIIDPFLRGNPAAVTKPEEV-KTDMVLLTHAHVDHILDAAP 84 M ++ R V ++ Sbjct: 1 MSDWNPSLYLHFSA-ERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARIT 59 Query: 85 IALQNNVPIVANVELAGYMEGQGVQTIGMNIGGTVDLGFAKATMIHAFHSSGITLEDGQK 144 + I + +DL FA A++ + Sbjct: 60 GIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELF--PHLV 117 Query: 145 AIYGGMPAGFIIQAEGRTVVHTGDTGLFGDLKMFGELYDIDLAILPIGGHYTMGPEHALI 204 ++ + + + + + + + + + Sbjct: 118 SLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPD-RGREPLAGVHAYYDILSEAGCEV 176 Query: 205 AAKWLGAKQVLPIHYNTFPPIVQDANAFVRALGEEGIRGTVLGYGESLEL 254 +P H + E + L + Sbjct: 177 DIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLE 226 >PAB0339 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Length = 252 Score = 176 bits (445), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 12/234 (5%), Positives = 32/234 (13%), Gaps = 15/234 (6%) Query: 26 MKIIYHGHSCIQIEVSGKSLIIDPFLRGNPAAVTKPEEVK-TDMVLLTHAHVDHILDAAP 84 + E S + + + Sbjct: 2 SDWNPSLYLHFSAERS-RPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 85 IALQNNVPIVANVELAGYM--EGQGVQTIGMNIGGT-VDLGFAKATMIHAFHSSGITLED 141 I + A L E + + + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASL--QWLPDHYEL----FP 114 Query: 142 GQKAIYGGMPAGFIIQAEGRTVVHTGDTGLFGDLKMFGELY-DIDLAILPIGGHYTMGPE 200 ++ P G + + + + + + Sbjct: 115 HLVSL--LNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEA 172 Query: 201 HALIAAKWLGAKQVLPIHYNTFPPIVQDANAFVRALGEEGIRGTVLGYGESLEL 254 + +P H + E + L Sbjct: 173 GCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTES-EQQLFLKRYHQML 225 >MA1308 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Length = 252 Score = 172 bits (437), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 10/230 (4%), Positives = 32/230 (13%), Gaps = 4/230 (1%) Query: 26 MKIIYHGHSCIQIEVSGKSLIIDPFLRGNPAAVTKPEEVKTDMVLLTHAHVDHILDAAPI 85 + H + ++ N V + L Sbjct: 4 WNPSLYLHFSAERSRP-AVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGID 62 Query: 86 ALQNNVPIVANVELAGYMEGQGVQTIGMNIGGTVDLGFAKATMIHAFHSSGITLEDGQKA 145 + + + ++ + A + + Sbjct: 63 SSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFP--HLVSLL 120 Query: 146 IYGGMPAGFIIQAEGRTVVHTGDTGLFGDLKMFGELYDIDLAILPIGGHYTMGPEHALIA 205 G+ A + + + G E + Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 206 AKWLGAKQVLPIHYNTFPPIVQDANAFVRAL-GEEGIRGTVLGYGESLEL 254 + + ++ E Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYP 230 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.141 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,559,511 Number of extensions: 856578 Number of successful extensions: 3231 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 3180 Number of HSP's successfully gapped: 30 Length of query: 254 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 150 Effective length of database: 26,383,695 Effective search space: 3957554250 Effective search space used: 3957554250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)