BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0004.p (501 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SSO1663 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase,... 374 e-103 mlr4786 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase,... 340 4e-93 mlr9030 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase,... 334 2e-91 APE1328 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase,... 332 1e-90 APE2528 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase,... 290 3e-78 >SSO1663 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 683 Score = 374 bits (960), Expect = e-103, Method: Compositional matrix adjust. Identities = 195/456 (42%), Positives = 287/456 (62%), Gaps = 17/456 (3%) Query: 10 LGIDVGGTFTDLAALDER-GNITATKTPSTPD-PSDGVIQGIEKLSRQLNISMLDFLTRC 67 +GID+GG FTD+ +E G I+ K +TPD PS+GV++ I++ L Sbjct: 4 VGIDIGGAFTDVVVYNEENGEISWAKVETTPDDPSNGVLEAIDEAKVNLGY--------V 55 Query: 68 SLLVHGTTVATNTILEYNGAKVGLITTEGFRDEIEFRRAYKESVFSPRLPAPYPIVPRRL 127 + ++HG T+A NTI+E GAKVGLITT+GFRD +E +RA + +++ R P P VPR L Sbjct: 56 NTIIHGQTLAINTIVERKGAKVGLITTKGFRDILEIQRANRRDMYNFRYKKPTPFVPRYL 115 Query: 128 RIGVPERVGAQGQTIIPLDEAAVREAIRFFKEEKVEAIAVSFLFSFLDPAHEQKVKQIAQ 187 R+ V ER+ + G + L+E V EAI+ K E VEAIAVSF+ S+++P HE KV +I + Sbjct: 116 RLEVTERIKSNGDILTSLNENEVVEAIKKLKAENVEAIAVSFINSYVNPIHELKVGEIIK 175 Query: 188 EEAPDIFVTLSSEVLPQIREFERVSTTIVNAYTAPAMKRYMERLDRRLREQQFKGELFVM 247 P+I VTLS EV + RE+ER ST ++NAY P M +Y+ +L+ + + FKG F M Sbjct: 176 RVDPNIIVTLSHEVTREWREYERTSTAVLNAYVMPKMSKYLSKLENEFKNRGFKGNYFAM 235 Query: 248 QSSGGVQNIVQSGQLAASCLLSGPAGGVTAAAFIGERIGYKDLITVDMGGTSYDVSVIEQ 307 S+GG+ + + L SGP GV A I + +G K++I +D G T+ S++ Sbjct: 236 LSNGGMATFDYAKRFPIYTLESGPVAGVIGAIKISDILGEKNIIAMDGGSTTTKASLVRN 295 Query: 308 LQPTLTTESWISR------YRVALPMLDIHTVGAGGGSIAWIDNGGMLQVGPRSAGSTPG 361 L+P + T+ ++ R Y V +P LDI +G GG SIA ID L+VGPR+AG+ PG Sbjct: 296 LEPNINTDYYVGRDKYNPGYPVKVPTLDIVEIGNGGTSIARIDETSNLKVGPRAAGAYPG 355 Query: 362 PACYGRGGTEPTVTDANVVLGYINPDNFLGGEMKLDRTLSEEAIRRAVAEPLGVSVIEAA 421 P YG+GG + TVTDA +V G++N + LGG++K+++ L+EEAI +A+ +S+ E + Sbjct: 356 PVAYGKGGKDVTVTDAYIVCGFLNQEELLGGKIKVNKRLAEEAISN-IAKYYNMSIEEVS 414 Query: 422 LAISEIVNNNMSNAIRFVTTQRGYDPRKFALLAAGG 457 I +I N+N NA+R ++ QRGYDPR+F L+A GG Sbjct: 415 YGIVKIANDNAVNAVRLISVQRGYDPREFTLVAYGG 450 >mlr4786 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 694 Score = 340 bits (871), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 196/466 (42%), Positives = 278/466 (59%), Gaps = 35/466 (7%) Query: 7 SWMLGIDVGGTFTDLAALDER--GNITATKTPSTPD-PSDGVIQGIEKLSRQLNISMLDF 63 S GIDVGGTFTDL +D R G + KTP+T D + GV+ + + I +D Sbjct: 15 SVAAGIDVGGTFTDLLLIDGRDGGKVHIAKTPTTVDNQAFGVVSALG--ATGFAIDGID- 71 Query: 64 LTRCSLLVHGTTVATNTILEYNGAKVGLITTEGFRDEIEFRRAYKESVFSPRLPAPY--- 120 L+VHGTT TN +LE A+ G+ITT GFRD IE R + P PY Sbjct: 72 -----LIVHGTTTTTNAVLERRLARTGMITTRGFRDVIELGRRTR--------PQPYGMT 118 Query: 121 ----PIVPRRLRIGVPERVGAQGQTIIPLDEAAVREAIRFFKEEKVEAIAVSFLFSFLDP 176 P++PR LR+ V ERV A G PLDEA +REA+ E++ + FL S+ +P Sbjct: 119 GTFVPVIPRNLRLEVSERVEASGAVRTPLDEAEMREAVTALIAAGCESLVIHFLHSYANP 178 Query: 177 AHEQKVKQIAQEEAPDIFVTLSSEVLPQIREFERVSTTIVNAYTAPAMKRYMERLDRRLR 236 AHE++ +IA E P+ ++T +L + REFER T VNA P ++RY+ERL + L Sbjct: 179 AHERRAAEIAAELWPNGYITTGHALLSEAREFERGVTASVNASVQPILERYVERLRKELA 238 Query: 237 EQQFKGELFVMQSSGGVQNIVQSGQLAASCLLSGPAGGVTAAAFIGERIGYKDLITVDMG 296 + + + +M +GG+ + + +A ++SGPA GV AAA+ G+R G+++L+T DMG Sbjct: 239 AKGYARDFLIMNGNGGMISARFVTRESAKTVMSGPASGVIAAAYTGKRAGFENLVTYDMG 298 Query: 297 GTSYDVSVIEQLQPTLTTESWISRYR-VALPMLDIHTVGAGGGSIAWIDNGGMLQVGPRS 355 GTS DV++I +P ++ E I + +PM+ +HTVGAGGGSIA +D G++Q+GP S Sbjct: 299 GTSTDVALIRNAEPAVSNEIEIEYAMPIHVPMVAVHTVGAGGGSIARVDAAGLIQIGPES 358 Query: 356 AGSTPGPACYGRGGTEPTVTDANVVLGYINPDNFLGGEMKLDRTLSEEAIRRAVAEPL-- 413 AG+ PGP CYGRGG EPT+TDAN+VLG + P L +D ++ E + A+ + Sbjct: 359 AGANPGPICYGRGGLEPTITDANLVLGRLAPKKLLA----VDNPVTVERVTGIFADKIGK 414 Query: 414 --GVSVIEAALAISEIVNNNMSNAIRFVTTQRGYDPRKFALLAAGG 457 G+S +EAA A+ + N M+ AIR V+ RG+DPR FAL A GG Sbjct: 415 ATGLSGVEAAGAVLRLGNMKMAGAIRMVSVSRGHDPRDFALFAFGG 460 >mlr9030 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 669 Score = 334 bits (857), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 188/453 (41%), Positives = 265/453 (58%), Gaps = 14/453 (3%) Query: 8 WMLGIDVGGTFTDLAALDE-RGNITATKTPSTPDP-SDGVIQGIEKLSRQLNISMLDFLT 65 W +G+DVGGTFTDL A++ G K +TP+ S GV+ I + N Sbjct: 2 WKVGVDVGGTFTDLFAVNTFTGEERTGKVLTTPEERSKGVLNAIANAELKPN-------- 53 Query: 66 RCSLLVHGTTVATNTILEYNGAKVGLITTEGFRDEIEFRRAYKESVFSPRLPAPYPIVPR 125 SLLVHGTT ATN ++E + +ITTEGFRD +E R ++E ++ P P P++ R Sbjct: 54 EISLLVHGTTTATNALIERSFPDAAMITTEGFRDVLEIGRMHREHLYRPYQTKPAPLIRR 113 Query: 126 RLRIGVPERVGAQGQTIIPLDEAAVREAIRFFKEEKVEAIAVSFLFSFLDPAHEQKVKQI 185 R R + ER A+G+ +D A ++ I E + ++AV + S+ +PA+E+KV +I Sbjct: 114 RYRYAIAERTNAKGEVEEDIDAAQLKPIIASISEAGIGSVAVCLINSYANPANERKVAKI 173 Query: 186 AQEEAPDIFVTLSSEVLPQIREFERVSTTIVNAYTAPAMKRYMERLDRRLREQQFKGELF 245 E P+I VT S++V P RE R + + A P M Y ERL RL E F G L Sbjct: 174 LSEALPNIRVTTSADVKPVFREHTRFTLASIRATILPVMADYFERLQARLLEGGFSGNLM 233 Query: 246 VMQSSGGVQNIVQSGQLAASCLLSGPAGGVTAAAFIGERIGYKDLITVDMGGTSYDVSVI 305 +++S+GG+ + Q+ + SGPAGGV A I G+ ++I DMGGTS+D S+I Sbjct: 234 ILKSNGGMMGVDQARLRVEELVESGPAGGVGYATEIARTAGFPNIIHTDMGGTSFDASII 293 Query: 306 EQLQPTLTTESWIS-RYRVALPMLDIHTVGAGGGSIAWIDNGGMLQVGPRSAGSTPGPAC 364 E Q +T E I VA+PMLDI ++GAGGGS+ W+D+GG L+VGP SAGS PGPAC Sbjct: 294 EDGQGLITREYEIEFDMPVAVPMLDIRSIGAGGGSVGWVDSGGSLRVGPMSAGSVPGPAC 353 Query: 365 YGRGGTEPTVTDANVVLGYINPDNFLGGEMKLDRTLSEEAIRRAVAEPLGVSVIEAALAI 424 YGRGGT P +TDAN+VLG I+PD L G+ KLD + +AI +A L +++ + A + Sbjct: 354 YGRGGTRPAITDANLVLGRISPD--LSGKFKLDVQAARDAI-ETIATKLNLTIEQCAEGM 410 Query: 425 SEIVNNNMSNAIRFVTTQRGYDPRKFALLAAGG 457 I M+ A++ V RG DPR + + GG Sbjct: 411 IRIATEAMAQAVKLVLASRGRDPRDYCFASFGG 443 >APE1328 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 785 Score = 332 bits (850), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 200/464 (43%), Positives = 265/464 (57%), Gaps = 28/464 (6%) Query: 10 LGIDVGGTFTDLAALDERGNITATKTPSTPD-PSDGVIQGIEKLSRQLNISMLDFLTRCS 68 +G+DVGGTFTD+ D G + K +TP P +GV+ G LSR L L Sbjct: 111 VGVDVGGTFTDIVVFD--GGLRVLKVLTTPKRPVEGVMNG---LSRALGT-----LEGVE 160 Query: 69 LLVHGTTVATNTILEYNG---AKVGLITTEGFRDEIEFRRAYKESVFSPRLPAPYPIVPR 125 +LVH TT+ TN L G ++ L EGFRD +E R + S+++P P P+VPR Sbjct: 161 VLVHATTIGTNLFLGQVGLQAPRIALFVNEGFRDIVEIGRQNRPSLYNPLYRRPKPLVPR 220 Query: 126 RLRIGVPERVGAQGQTIIPLDEAAVREAIRFFKEEKVEAIAVSFLFSFLDPAHEQKVKQI 185 LRIGV R+G G I LD AVRE E V AVSFL S +P HE+ Q Sbjct: 221 DLRIGVRGRIGPDGVEIERLDVDAVREWAARLCREGVRVYAVSFLHSHRNPLHEKMAAQA 280 Query: 186 AQEEAPDIFVTLSSEVLPQIREFERVSTTIVNAYTAPAMKRYMERLDRRLREQQFKGELF 245 E P +V EV PQ E+ER STT+VNA P + Y++ L R+LR++ FKG L Sbjct: 281 VGEACPGAYVVTGHEVDPQPMEYERTSTTVVNAVLKPILSTYLQELHRQLRDRGFKGVLL 340 Query: 246 VMQSSGGVQNIVQSGQLAASCLLSGPAGGVTAAAFIGERIGYKDLITVDMGGTSYDVSVI 305 VMQS+GGV + ++ A+ + SGP+ G A A+ + DMGGT+ S I Sbjct: 341 VMQSNGGVAGVEEAVGRPAAFIESGPSAGAVAVAYFSRLQRVDRALGFDMGGTTAKASSI 400 Query: 306 EQLQPTLTTESWI------------SRYRVALPMLDIHTVGAGGGSIAWIDNGGMLQVGP 353 +P + + S Y V P +D+ V AGGG+IAW+D GG LQVGP Sbjct: 401 IDGEPEVVDTYEVGGRVHMGRLIRGSGYPVRYPHIDLAEVSAGGGTIAWVDPGGALQVGP 460 Query: 354 RSAGSTPGPACYGRGGTEPTVTDANVVLGYINPDNFLGGEMKLDRTLSEEAIRRAVAEPL 413 SAG+ PGPACYG GG++PTVTDAN++LG + PD GG ++L R L+E+A++ VAEP+ Sbjct: 461 MSAGADPGPACYGLGGSKPTVTDANLILGRL-PDVLAGG-LRLSRRLAEDAVKGRVAEPM 518 Query: 414 GVSVIEAALAISEIVNNNMSNAIRFVTTQRGYDPRKFALLAAGG 457 G+ V+EAA AI I N MS A+R V+ +RG DPR F + A GG Sbjct: 519 GLDVVEAAWAIVRIANAIMSRALRLVSLERGRDPRGFTMYAFGG 562 >APE2528 [EQ] COG0145 N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 687 Score = 290 bits (743), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 181/471 (38%), Positives = 262/471 (55%), Gaps = 34/471 (7%) Query: 8 WML--GIDVGGTFTDLAALDE-RGNITATKTPSTP-DPSDGVIQGIEKLSRQLNISMLDF 63 W++ G+D+GGTFTDL DE G + + K +TP +P GVI+ +E+ S ++I +D Sbjct: 5 WLIRVGVDIGGTFTDLIVFDEGSGRLESLKVLTTPREPWVGVIRALEEAS--VDIEGVD- 61 Query: 64 LTRCSLLVHGTTVATNTILEYNGAK---VGLITTEGFRDEIEFRRAYKESVFSPRLPAPY 120 +++H TT+ TN L G + LIT GFRD +E R + +++ P Sbjct: 62 -----VVIHATTLGTNMFLGQEGLEPPSAVLITNRGFRDVLEIGRQNRPELYNLYFTRPK 116 Query: 121 PIVPRRLRIGVPERVGAQGQTIIPLDEAAVREAIRFFKE--EKVEAIAVSFLFSFLDPAH 178 P+VPR R+ V R+ A+G + +D E R KE + + VS L S ++P H Sbjct: 117 PLVPRDRRLVVKGRIDARGNVVEDIDRG---EVARLAKEWCGRADVFIVSMLNSHINPVH 173 Query: 179 EQKVKQIAQEEAPDIFVTLSSEVLPQIREFERVSTTIVNAYTAPAMKRYMERLDRRLREQ 238 E ++ I E P V + + PQ +E+ER ST +VN P + +Y++RL L + Sbjct: 174 EDEIGSIITRECPGAIVVEGARIDPQEKEYERTSTAVVNGLLKPILSKYLKRLKSDLEGR 233 Query: 239 QFKGELFVMQSSGGVQNIVQSGQLAASCLLSGPAGGVTAAAFIGERIGYKDLITVDMGGT 298 FKG L +MQSSGGV + + + A+ + SGPA G A ++ G I DMGGT Sbjct: 234 GFKGILLLMQSSGGVSTVDYAVERPAAFIESGPAAGAIAVSYYATMQGVGRAIGFDMGGT 293 Query: 299 SYDVSVIEQLQPTLTTESWI------------SRYRVALPMLDIHTVGAGGGSIAWIDNG 346 + S I +P + E + S Y V P +D+ V AGGG+IAW+D G Sbjct: 294 TAKASSIIDGEPLVVREYEVGAKVHMGRPLRGSGYPVRYPYIDLVEVSAGGGTIAWVDPG 353 Query: 347 GMLQVGPRSAGSTPGPACYGRGGTEPTVTDANVVLGYINPDNFLGGEMKLDRTLSEEAIR 406 G ++VGP SAG+ PGPACYG GG +PT+TDAN+VLG + P+ G +KL R L+ A+ Sbjct: 354 GAVRVGPVSAGADPGPACYGLGGDKPTLTDANLVLGRL-PEALAWGRIKLYRDLAVRAL- 411 Query: 407 RAVAEPLGVSVIEAALAISEIVNNNMSNAIRFVTTQRGYDPRKFALLAAGG 457 ++A+ G+ V EAA A+ I N M A+R VT +RGYDPR+F L A GG Sbjct: 412 ASIADKAGLEVDEAAWAVIRIANTVMGKALRLVTVERGYDPREFTLYAFGG 462 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,694,085 Number of extensions: 1728457 Number of successful extensions: 3770 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 3637 Number of HSP's successfully gapped: 32 Length of query: 501 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 390 Effective length of database: 25,576,357 Effective search space: 9974779230 Effective search space used: 9974779230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)