BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0033.p (241 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yetF [S] COG2323 Predicted membrane protein 180 1e-45 BH1565 [S] COG2323 Predicted membrane protein 159 4e-39 CAC1521 [S] COG2323 Predicted membrane protein 124 1e-28 BH1970 [S] COG2323 Predicted membrane protein 123 2e-28 BS_ydfS [S] COG2323 Predicted membrane protein 118 7e-27 >BS_yetF [S] COG2323 Predicted membrane protein Length = 231 Score = 180 bits (457), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 92/211 (43%), Positives = 137/211 (64%) Query: 9 DYVSVLVKLTAGFVGLWAMARLLGKKEISALTPFDFISAVILGDLVGNTIYEPDKSVLLL 68 +Y+SV V+L G L+ + +LLGK + S +TPFDFISA+ILG+LVGN +Y+ + + + Sbjct: 3 NYLSVAVELVCGLGILFIILKLLGKTQFSQITPFDFISALILGELVGNAVYDHEIKIKEI 62 Query: 69 LFALAVWTVLSIIFEKITQRVRKLRKPLEGEPEILIRDGKLDLTKLRKNNLDFEQLRMML 128 +FA +W VL I E ITQ+++ RK LEGEP I+IR G+L ++KN +D QL+ +L Sbjct: 63 IFASLLWGVLIYIIEFITQKMKSSRKFLEGEPNIVIRKGELQYKVMKKNKIDINQLQSLL 122 Query: 129 RAKDTFSMNEVAYAIYETNGSLSILKKPQFEPATRKELDIKPQHTRLPRSLVEAGEVQRA 188 R +FS+ EV YAI ETNG +S+L K F+ T K+L I + LP +L+ GE+ R Sbjct: 123 RQAGSFSIQEVEYAILETNGMVSVLPKSDFDKPTNKDLQIPSKSVSLPITLIIDGEIVRD 182 Query: 189 ALASLGHDETWLRRKLQEEGCPNLDSVAYAE 219 L G DE WL+++L+++ + V +AE Sbjct: 183 NLKEAGVDEQWLKQELKKKNIDKTEDVLFAE 213 >BH1565 [S] COG2323 Predicted membrane protein Length = 221 Score = 159 bits (402), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 87/217 (40%), Positives = 136/217 (62%), Gaps = 6/217 (2%) Query: 9 DYVSVLVKLTAGFVGLWAMARLLGKKEISALTPFDFISAVILGDLVGNTIYEPDKSVLLL 68 ++V++ V+L GF L + ++LGK +I+ LTPFDFISA++LG+LVGN IY+ D +L + Sbjct: 4 NFVALSVELFVGFWALLVLMKVLGKSQINQLTPFDFISALVLGELVGNAIYDKDIGILYV 63 Query: 69 LFALAVWTVLSIIFEKITQRVRKLRKPLEGEPEILIRDGKLDLTKLRKNNLDFEQLRMML 128 L+A++ W +L E ITQ+ R LEG P+++I DGK+ +L+++ LD +QL M+L Sbjct: 64 LYAISAWGLLIYTVEIITQKFPITRPFLEGSPKLIINDGKIMYHELKRSKLDIDQLAMLL 123 Query: 129 RAKDTFSMNEVAYAIYETNGSLSILKKPQFEPATRKELDIKPQHTRLPRSLVEAGEVQRA 188 R K+ FS+ EV Y I E+NGS+S++KK ++ T +L K LP +V G + Sbjct: 124 REKEVFSIREVHYGILESNGSISVVKKQGYQ-TTNGQLTYKT----LPIVVVADGVLNEQ 178 Query: 189 ALASLGHDETWLRRKLQEEGCPNLDSVAYAE-INEEG 224 L G + L+++G N SV YAE ++E+G Sbjct: 179 NLEKAGMTKEAFNDSLRKQGISNYKSVLYAEYLSEDG 215 >CAC1521 [S] COG2323 Predicted membrane protein Length = 242 Score = 124 bits (312), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 71/216 (32%), Positives = 109/216 (50%) Query: 13 VLVKLTAGFVGLWAMARLLGKKEISALTPFDFISAVILGDLVGNTIYEPDKSVLLLLFAL 72 V+V+ GF + A++LGK++IS LT FD+I + +G + + L Sbjct: 7 VIVRSIIGFFTMLIFAKILGKQQISQLTFFDYILGITIGSIAATLTTDLSSRAWPHWVGL 66 Query: 73 AVWTVLSIIFEKITQRVRKLRKPLEGEPEILIRDGKLDLTKLRKNNLDFEQLRMMLRAKD 132 W +L + E I+ + R K LEGEP I+I GK+ ++K N + +LR KD Sbjct: 67 LTWALLGYLMEYISTKWRYAGKYLEGEPTIVIMKGKIMEDAMKKMNFRATDIMELLRDKD 126 Query: 133 TFSMNEVAYAIYETNGSLSILKKPQFEPATRKELDIKPQHTRLPRSLVEAGEVQRAALAS 192 F +NEV +AI E NG LS+LKKP+ +P T +++I + L+ G + L Sbjct: 127 VFDLNEVDFAIIEPNGKLSVLKKPELQPVTCNDMNITKNEMGISTELIYDGILIEENLRQ 186 Query: 193 LGHDETWLRRKLQEEGCPNLDSVAYAEINEEGKLAI 228 L + WL + L+ G N+ V A +N G L I Sbjct: 187 LNKTKDWLFKVLESRGIKNVSEVFLATLNPGGSLYI 222 >BH1970 [S] COG2323 Predicted membrane protein Length = 292 Score = 123 bits (309), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 59/220 (26%), Positives = 120/220 (54%) Query: 9 DYVSVLVKLTAGFVGLWAMARLLGKKEISALTPFDFISAVILGDLVGNTIYEPDKSVLLL 68 D +V ++ GF+ L+ +AR++GKK I+ +T +++I + +G + + P + Sbjct: 3 DLGNVFIRGVVGFIVLFILARIMGKKHITDMTYYEYIVGIAIGSIAAELTFSPHVRMSNF 62 Query: 69 LFALAVWTVLSIIFEKITQRVRKLRKPLEGEPEILIRDGKLDLTKLRKNNLDFEQLRMML 128 LF + +W + ++ K+ + + R EGEP +LI +G + L+K NL ++L + L Sbjct: 63 LFGMIIWAIFPMVLSKLELKSFRFRSLSEGEPTVLIENGNILEENLKKENLTVDELMIHL 122 Query: 129 RAKDTFSMNEVAYAIYETNGSLSILKKPQFEPATRKELDIKPQHTRLPRSLVEAGEVQRA 188 R KD F +++V A+ E +G +S++KK +P T K+ + + P+ ++ G V Sbjct: 123 RQKDAFKLDDVESAVMEKSGLISVMKKSNVQPLTPKDAGLVIELDHSPQIVMIDGNVLEK 182 Query: 189 ALASLGHDETWLRRKLQEEGCPNLDSVAYAEINEEGKLAI 228 +L G+ + WL ++ ++G + V A+I+ +G + + Sbjct: 183 SLQDYGYTKEWLLAEVTKQGAKRFEDVFLAQIDSKGNVYV 222 >BS_ydfS [S] COG2323 Predicted membrane protein Length = 235 Score = 118 bits (296), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 74/201 (36%), Positives = 114/201 (56%), Gaps = 9/201 (4%) Query: 11 VSVLVKLTAGFVGLWAMARLLGKKEISALTPFDFISAVILGDLVG----NTIYEPDKSVL 66 + V+++ A F L R+LGK+ IS +T FDFI+A+ LG + NT +P + Sbjct: 4 LEVVIRTVASFGLLLIAERILGKQTISQMTIFDFIAAITLGAIAAGLAYNTSIKPHN--M 61 Query: 67 LLLFALAVWTVLSIIFEKITQRVRKLRKPLEGEPEILIRDGKLDLTKLRKNNLDFEQLRM 126 + F++ V T+ I F I R KLRK G+P +LI++GK+ + +RK + L Sbjct: 62 AISFSIFVLTIFLISFLSIKNR--KLRKFFAGDPTVLIQNGKILESNMRKMRYTLDYLNQ 119 Query: 127 MLRAKDTFSMNEVAYAIYETNGSLSILKKPQFEPATRKELDIK-PQHTRLPRSLVEAGEV 185 LR K+ F++ EV +AI ETNG L++L+KPQF T+++L I Q RLP L+ GE+ Sbjct: 120 QLREKEIFNIEEVLFAILETNGQLTVLRKPQFRHVTKQDLMIAVNQEQRLPIELIMDGEI 179 Query: 186 QRAALASLGHDETWLRRKLQE 206 L E+WL +L++ Sbjct: 180 IENNLKQNRLTESWLLEELRK 200 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,301,906 Number of extensions: 661067 Number of successful extensions: 1381 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 1373 Number of HSP's successfully gapped: 16 Length of query: 241 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 138 Effective length of database: 26,499,029 Effective search space: 3656866002 Effective search space used: 3656866002 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)