BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0027.p (248 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_map [J] COG0024 Methionine aminopeptidase 282 3e-76 PA2748 [J] COG0024 Methionine aminopeptidase 253 2e-67 alr4150 [J] COG0024 Methionine aminopeptidase 248 5e-66 slr0918 [J] COG0024 Methionine aminopeptidase 241 9e-64 SMc00298 [J] COG0024 Methionine aminopeptidase 239 2e-63 >BS_map [J] COG0024 Methionine aminopeptidase Length = 248 Score = 282 bits (722), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 130/248 (52%), Positives = 176/248 (70%) Query: 1 MIIMKTMEEIEKMRAAGKILAECHRQIAQMIKPGITTWEIDQFAEKFILSQGATPEQKGY 60 MII KT E+ MR AG+I+A H ++ + IKPGI+T E+DQ AE+FI QGA P KGY Sbjct: 1 MIICKTPRELGIMREAGRIVALTHEELKKHIKPGISTKELDQIAERFIKKQGAIPSFKGY 60 Query: 61 HGYPYATCASVNDVICHGFPKREELKDGDIVTIDMVVNLNGWLADSAWSYAVGNISEQAE 120 +G+ + C SVN+ + HG P LKDGDI++ID+ LNG+ DSAW+Y VGNIS+ + Sbjct: 61 NGFRGSICVSVNEELVHGIPGSRVLKDGDIISIDIGAKLNGYHGDSAWTYPVGNISDDDK 120 Query: 121 KLLNTTKESLFKGIEQAVAGNRIGDVAHAVQVYAESNGFSVVRDFIGHGIGSEMHEAPEV 180 KLL T+ESL+KG+++A G R+ +++HA+Q Y E+ FSVVR+++GHG+G ++HE P++ Sbjct: 121 KLLEVTEESLYKGLQEAKPGERLSNISHAIQTYVENEQFSVVREYVGHGVGQDLHEDPQI 180 Query: 181 PHYGPAGKGPRIKEGMVFTIEPMLNTGSYRTKVDADGWTARTIDGGLSAQYEHTLAITPQ 240 PHYGP KGPR+K GMV IEPM+N GS K AD WT T+DG A +EHT+AIT Sbjct: 181 PHYGPPNKGPRLKPGMVLAIEPMVNAGSRYVKTLADNWTVVTVDGKKCAHFEHTIAITET 240 Query: 241 GTIILTEL 248 G ILT + Sbjct: 241 GFDILTRV 248 >PA2748 [J] COG0024 Methionine aminopeptidase Length = 260 Score = 253 bits (646), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 117/246 (47%), Positives = 172/246 (69%), Gaps = 2/246 (0%) Query: 3 IMKTMEEIEKMRAAGKILAECHRQIAQMIKPGITTWEIDQFAEKFIL-SQGATPEQKGYH 61 ++K+ EEI++M AG + A+ + ++++PGI+T EID+F E++I+ + A P KG + Sbjct: 1 MIKSAEEIQRMSVAGALTAKVLEALDEVVRPGISTAEIDRFCERYIVDTLAAIPGSKGQY 60 Query: 62 GYPYATCASVNDVICHGFPKREE-LKDGDIVTIDMVVNLNGWLADSAWSYAVGNISEQAE 120 GYP+ SVN+V+CHG+P ++ L+DGDIV +D+ V +G+ DS+ Y VG I QA+ Sbjct: 61 GYPFTVNTSVNEVVCHGWPSAQQILRDGDIVNVDVTVIKDGYFGDSSKMYRVGAIDRQAQ 120 Query: 121 KLLNTTKESLFKGIEQAVAGNRIGDVAHAVQVYAESNGFSVVRDFIGHGIGSEMHEAPEV 180 KLL+ T+E L++ I +GD+ HA+Q +AE+NG+SVVR++ GHGIG +MHEAP+V Sbjct: 121 KLLDVTRECLYRAIRVVRPDATLGDIGHAIQSHAEANGYSVVREYCGHGIGRKMHEAPQV 180 Query: 181 PHYGPAGKGPRIKEGMVFTIEPMLNTGSYRTKVDADGWTARTIDGGLSAQYEHTLAITPQ 240 H+G AG G R+K GM FTIEPM+N G + K+ DGWT T D LSAQYEHT+ +T + Sbjct: 181 LHFGRAGAGARLKAGMTFTIEPMINQGGHAVKLHKDGWTVTTRDRRLSAQYEHTVLVTER 240 Query: 241 GTIILT 246 G +LT Sbjct: 241 GCRVLT 246 >alr4150 [J] COG0024 Methionine aminopeptidase Length = 359 Score = 248 bits (634), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 10/247 (4%), Positives = 36/247 (14%), Gaps = 1/247 (0%) Query: 1 MIIMKTMEEIEKMRAAGKILAECHRQIAQMIKPGITTWEIDQFAEKFILSQGATPEQKGY 60 +++ ++ ++ + G ++ Sbjct: 5 TVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEAL 64 Query: 61 HGYPYATCASVNDVICHGFPKREE-LKDGDIVTIDMVVNLNGWLADSAWSYAVGNISEQA 119 V + + + T L +++ V + Sbjct: 65 RLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLD 124 Query: 120 EKLLNTTKESLFKGIEQAVAGNRIGDVAHAVQVYAESNGFSVVRDFIGHGIGSEMHEAPE 179 + V + + Sbjct: 125 TGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEG 184 Query: 180 VPHYGPAGKGPRIKEGMVFTIEPMLNTGSYRTKVDADGWTARTIDGGLSAQYEHTLAITP 239 P I + + + + G + I Sbjct: 185 KPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAV 244 Query: 240 QGTIILT 246 + Sbjct: 245 REHKAGE 251 >slr0918 [J] COG0024 Methionine aminopeptidase Length = 253 Score = 241 bits (614), Expect = 9e-64, Method: Compositional matrix adjust. Identities = 121/246 (49%), Positives = 167/246 (67%), Gaps = 1/246 (0%) Query: 2 IIMKTMEEIEKMRAAGKILAECHRQIAQMIKPGITTWEIDQFAEKFILSQGATPEQKGYH 61 I + + EIEKMR AG++ A +A M++PGITT E++ AEK+ + GA GY+ Sbjct: 5 ITLLSRREIEKMRQAGQLAAALLDHLAPMVQPGITTLELNDEAEKWTKAHGAISAPLGYN 64 Query: 62 GYPYATCASVNDVICHGFPKREE-LKDGDIVTIDMVVNLNGWLADSAWSYAVGNISEQAE 120 G+P + C S+N+VICHG P R+ L+ GDI+ +D+ ++G+ D + ++ VG S AE Sbjct: 65 GFPKSICTSINEVICHGIPHRKRVLQAGDIINVDVTPIVDGYHGDCSRTFFVGTPSPVAE 124 Query: 121 KLLNTTKESLFKGIEQAVAGNRIGDVAHAVQVYAESNGFSVVRDFIGHGIGSEMHEAPEV 180 KL+ T+E L GIE G +IGD+ A+Q +AE+ GFSVVRDF+GHGI H AP++ Sbjct: 125 KLVKVTEECLRLGIEAVKPGGKIGDIGAAIQSHAEAQGFSVVRDFVGHGISKIFHTAPQI 184 Query: 181 PHYGPAGKGPRIKEGMVFTIEPMLNTGSYRTKVDADGWTARTIDGGLSAQYEHTLAITPQ 240 PHYG AGKG R++ GMVFTIEPM+N G++ + DGWTA T DG LSAQ+EHT+A+T Sbjct: 185 PHYGKAGKGKRLRPGMVFTIEPMINEGTWEAVLLDDGWTAITKDGKLSAQFEHTIAVTED 244 Query: 241 GTIILT 246 G ILT Sbjct: 245 GVEILT 250 >SMc00298 [J] COG0024 Methionine aminopeptidase Length = 279 Score = 239 bits (611), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 114/239 (47%), Positives = 149/239 (62%) Query: 8 EEIEKMRAAGKILAECHRQIAQMIKPGITTWEIDQFAEKFILSQGATPEQKGYHGYPYAT 67 E E MR A ++ A C ++ + PG+TT EID+F +F + GA P Y GY ++ Sbjct: 24 EGFEGMRKACQVTARCLDELVARVSPGVTTDEIDRFVFEFGMDHGALPATLNYRGYTKSS 83 Query: 68 CASVNDVICHGFPKREELKDGDIVTIDMVVNLNGWLADSAWSYAVGNISEQAEKLLNTTK 127 C S+N V+CHG P + L++GDIV ID+ ++GW DS+ Y VG I AE+LL+ T Sbjct: 84 CTSINHVVCHGIPNDKPLREGDIVNIDVTYVVDGWHGDSSRMYPVGEIKRAAERLLDVTH 143 Query: 128 ESLFKGIEQAVAGNRIGDVAHAVQVYAESNGFSVVRDFIGHGIGSEMHEAPEVPHYGPAG 187 E L +GI G R G + A+Q YAE+ SVVRDF GHG+G H+AP + HYG A Sbjct: 144 ECLMRGIAAVRPGARTGAIGEAIQAYAEAERCSVVRDFCGHGVGRLFHDAPNILHYGSAN 203 Query: 188 KGPRIKEGMVFTIEPMLNTGSYRTKVDADGWTARTIDGGLSAQYEHTLAITPQGTIILT 246 GP +KEGM+FTIEPM+N G KV +DGWTA T D LSAQYEHT+ +T G I T Sbjct: 204 DGPELKEGMIFTIEPMINLGRPHVKVLSDGWTAVTRDRSLSAQYEHTVGVTADGCEIFT 262 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,535,655 Number of extensions: 827353 Number of successful extensions: 4317 Number of sequences better than 1.0e-05: 75 Number of HSP's gapped: 4224 Number of HSP's successfully gapped: 75 Length of query: 248 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 144 Effective length of database: 26,383,695 Effective search space: 3799252080 Effective search space used: 3799252080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)