BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0022.p (92 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all4160_1 [T] COG1366 Anti-anti-sigma regulatory factor (antagon... 51 4e-07 >all4160_1 [T] COG1366 Anti-anti-sigma regulatory factor (antagonist of anti-sigma factor) Length = 187 Score = 51.2 bits (121), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 3/90 (3%), Positives = 15/90 (16%), Gaps = 2/90 (2%) Query: 4 IVIDMPEMFSVEEASEFREKIRGHIDGGFSS--FVLDFGGCAFIDSTGLGVIVSSYKKCV 61 + ++A + + + A + ++ + Sbjct: 29 KKLFEMICLEGQQAGLSWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRH 88 Query: 62 ENGGTIKLKALNANVLKIFELTRLTHVFEI 91 + A + Sbjct: 89 RGKIQAIIGNARAYLQMEQNGEPFVDFVWS 118 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.142 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 6,482,119 Number of extensions: 209876 Number of successful extensions: 422 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 423 Number of HSP's successfully gapped: 3 Length of query: 92 Length of database: 38,378,431 Length adjustment: 62 Effective length of query: 30 Effective length of database: 31,227,723 Effective search space: 936831690 Effective search space used: 936831690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 109 (46.6 bits)