BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0004.p (519 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0789 [E] COG1113 Gamma-aminobutyrate permease and related perm... 166 6e-41 BS_ytnA [E] COG1113 Gamma-aminobutyrate permease and related per... 155 2e-37 YPO1937 [E] COG1113 Gamma-aminobutyrate permease and related per... 147 3e-35 YPO3854 [E] COG1113 Gamma-aminobutyrate permease and related per... 144 3e-34 ML1305 [E] COG1113 Gamma-aminobutyrate permease and related perm... 140 5e-33 >PA0789 [E] COG1113 Gamma-aminobutyrate permease and related permeases Length = 471 Score = 166 bits (421), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 107/329 (32%), Positives = 163/329 (49%), Gaps = 3/329 (0%) Query: 2 GKEEKGLSAWGLTMLALGTVVGGSFFLGSSVAIRAAGPSVLLSYVIGGILVYFILSALSE 61 G ++ L + ++ALG +G FLGS+ AI+ AGP++++SY++GG+ + I+ AL E Sbjct: 12 GSLKRELGERHIRLMALGACIGVGLFLGSAKAIQMAGPAIMISYILGGLAILVIMRALGE 71 Query: 62 MTVANPVAGSFRTYTEQAFGRGAGFTVGWVYWTGLVLAMSSEATAVSILLRGWFPALPLA 121 M V NPVAGSF Y + G AGF GW YW ++ +E TAV++ + WFP +P Sbjct: 72 MAVHNPVAGSFSQYAKDYLGPLAGFITGWNYWFLWLVTCVAEITAVAVYMGIWFPDVPRW 131 Query: 122 LLGSGIIAGVTLLNLLGAKRLSRLESXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRAVGL 181 + +A + +NL+ + E A G+ Sbjct: 132 IWALAALASMGTINLIAVRAFGEFEFWFALIKIVTILAMVVVGIGMIAFGFGNDGIATGI 191 Query: 182 DVVRGQSLF-PGGIAGIAGSMLMVMFTYAGFEVLGLAASETGNPGVTIPRAIRYTIVLLV 240 + F P GI G+ S+ MVMF Y G E++GL A E NP TIP AI ++ Sbjct: 192 SNLWAHGGFMPNGIQGVLMSLQMVMFAYLGVEMIGLTAGEARNPTKTIPSAINSVFWRIL 251 Query: 241 GLYLAAIIGLFLLLPPARVSEQVSPFVSALSLYGLGWAGTVMNIVLVSAILSTMLASVFG 300 Y+ ++ + L P + Q SPFV G+ A ++N V+++A LS+ +F Sbjct: 252 LFYVGSLFVIMSLYPWNEIGTQGSPFVMTFERMGIKTAAGIINFVVITAALSSCNGGIFS 311 Query: 301 LGRMLRSLAEEGHTPAWM--RDRADIPYR 327 GRML SLA+ PA+ R+ +P R Sbjct: 312 TGRMLYSLAQHRQAPAFFGKASRSGVPRR 340 >BS_ytnA [E] COG1113 Gamma-aminobutyrate permease and related permeases Length = 463 Score = 155 bits (391), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 100/316 (31%), Positives = 158/316 (50%), Gaps = 3/316 (0%) Query: 3 KEE--KGLSAWGLTMLALGTVVGGSFFLGSSVAIRAAGPSVLLSYVIGGILVYFILSALS 60 K+E +GL +++++LG +G FLGS+ AI+ AGP +L++Y G++++FI+ AL Sbjct: 5 KQELHRGLEERHISLMSLGAAIGVGLFLGSASAIQLAGPGILVAYAASGLVMFFIMRALG 64 Query: 61 EMTVANPVAGSFRTYTEQAFGRGAGFTVGWVYWTGLVLAMSSEATAVSILLRGWFPALPL 120 EM + PVAGSF Y G AG+ GW YW V+ +E TAV I + WFP +P Sbjct: 65 EMAIQKPVAGSFSRYARDYLGPLAGYLTGWNYWFLWVVTCMAEITAVGIYMGFWFPDVPN 124 Query: 121 ALLGSGIIAGVTLLNLLGAKRLSRLESXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRAVG 180 + + +T +N L K LE A G Sbjct: 125 WIWALSALVIMTGVNFLAVKAYGELEFWFALIKIVAILSMIAVGLLMIIAGVGNGGIATG 184 Query: 181 L-DVVRGQSLFPGGIAGIAGSMLMVMFTYAGFEVLGLAASETGNPGVTIPRAIRYTIVLL 239 + ++ FP G+ G+ S+ MVMF Y G E++G+ A E NP ++ +AI + Sbjct: 185 ISNLWNNGGFFPHGLKGVLLSLQMVMFAYLGIEMIGVTAGEVKNPQKSLAKAIDTVFWRI 244 Query: 240 VGLYLAAIIGLFLLLPPARVSEQVSPFVSALSLYGLGWAGTVMNIVLVSAILSTMLASVF 299 + Y+ A+ + + P + Q SPFV G+ A ++N V+++A LS+ + +F Sbjct: 245 LIFYVGALFVIMSIYPWQEIGSQGSPFVLTFQKVGIPSAAGIINFVVLTAALSSCNSGIF 304 Query: 300 GLGRMLRSLAEEGHTP 315 GRML +LAE+ P Sbjct: 305 STGRMLFNLAEQKEAP 320 >YPO1937 [E] COG1113 Gamma-aminobutyrate permease and related permeases Length = 508 Score = 147 bits (372), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 95/325 (29%), Positives = 164/325 (50%), Gaps = 5/325 (1%) Query: 13 LTMLALGTVVGGSFFLGSSVAIRAAGPSVLLSYVIGGILVYFILSALSEMTVANPVAGSF 72 + M+A+G +G FLG+ ++ AGP++ L Y++ GI +FIL AL E+ + P +GSF Sbjct: 34 IQMIAIGGSIGTGLFLGTGARLQMAGPALALVYLVCGIFSFFILRALGELVLHRPTSGSF 93 Query: 73 RTYTEQAFGRGAGFTVGWVYWTGLVLAMSSEATAVSILLRGW--FPALPLALLGSGIIAG 130 +Y + G A + GW+Y+ + + TAV++ + W F +P L G ++ Sbjct: 94 VSYAREFLGEKASYVAGWMYFLNWAMTGIVDITAVALYMHYWGTFSDVPQWLFALGALSI 153 Query: 131 VTLLNLLGAKRLSRLESXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRAVGLDVVRGQS-L 189 V +N++G + + +E G+ ++ Sbjct: 154 VATMNMIGVRWFAEMEFWFALIKVAAIALFLIVGVIFLGTGQSVAGHTTGIHLITDNGGF 213 Query: 190 FPGGIAGIAGSMLMVMFTYAGFEVLGLAASETGNPGVTIPRAIRYTIVLLVGLYLAAIIG 249 FP G+ + V+F +AG E++G AA E +P +P+AI I + Y+ +++ Sbjct: 214 FPHGLLPALIMVQGVIFAFAGIELIGTAAGECKDPEKMLPKAINSVIWRIGLFYVGSVVL 273 Query: 250 LFLLLPPARVSEQVSPFVSALSLYGLGWAGTVMNIVLVSAILSTMLASVFGLGRMLRSLA 309 L LLP SPFV+ S G+ + GT+MNIV++SA LS++ + ++ GR+LRSL+ Sbjct: 274 LVCLLPWHAYQAGQSPFVTFFSNLGVPYIGTIMNIVVLSAALSSLNSGLYSTGRILRSLS 333 Query: 310 EEGHTPAWMRDRA--DIPYRGILIS 332 G P +M + +PY GIL++ Sbjct: 334 MGGSAPKFMSKMSPQSVPYAGILVT 358 >YPO3854 [E] COG1113 Gamma-aminobutyrate permease and related permeases Length = 463 Score = 144 bits (364), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 97/313 (30%), Positives = 153/313 (48%), Gaps = 1/313 (0%) Query: 5 EKGLSAWGLTMLALGTVVGGSFFLGSSVAIRAAGPSVLLSYVIGGILVYFILSALSEMTV 64 +GL A + ++ALG +G F+GS+ ++ AGPSVLL+Y+I G+ V+FI+ A+ EM Sbjct: 11 HRGLEARHIELIALGGTIGVGLFMGSASTLKWAGPSVLLAYIIAGLFVFFIMRAMGEMLY 70 Query: 65 ANPVAGSFRTYTEQAFGRGAGFTVGWVYWTGLVLAMSSEATAVSILLRGWFPALPLALLG 124 PVAGSF Y + G+ W YW + SE TA+ + ++ WFP +P L Sbjct: 71 LEPVAGSFAVYAHKYLSPYFGYLTAWGYWFMWIAVGISEITAIGVYVQFWFPEIPQWLPA 130 Query: 125 SGIIAGVTLLNLLGAKRLSRLESXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRAVGLDVV 184 +A V L NL K LE + +G D + Sbjct: 131 IAGVAIVALANLAAVKLYGELEFWFAMIKVTTIIVMILVGLGVIFFGFGNHGQPIGFDNL 190 Query: 185 RGQ-SLFPGGIAGIAGSMLMVMFTYAGFEVLGLAASETGNPGVTIPRAIRYTIVLLVGLY 243 F GG G ++ +V+ +Y G E++G+ A E NP VT+ RAI + ++ Y Sbjct: 191 TAHGGFFAGGWKGFLFALCIVVASYQGVELVGITAGEARNPQVTLRRAINNILWRILIFY 250 Query: 244 LAAIIGLFLLLPPARVSEQVSPFVSALSLYGLGWAGTVMNIVLVSAILSTMLASVFGLGR 303 + AI + + P + + SPFV + G+ A ++N V+++A LS + ++ GR Sbjct: 251 VGAIFVIVTIFPWNGIGTEGSPFVLTFAKIGIVAAAGIINFVILTAALSGCNSGMYSGGR 310 Query: 304 MLRSLAEEGHTPA 316 ML +LA+ PA Sbjct: 311 MLYALAKNRQLPA 323 >ML1305 [E] COG1113 Gamma-aminobutyrate permease and related permeases Length = 505 Score = 140 bits (353), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 109/340 (32%), Positives = 165/340 (48%), Gaps = 8/340 (2%) Query: 1 MGKE---EKGLSAWGLTMLALGTVVGGSFFLGSSVAIRAAGPSVLLSYVIGGILVYFILS 57 +G+E KGL L M+ +G +G FLG+ + AGP + L Y + G+ V+ IL Sbjct: 20 LGEEAGYHKGLKPRQLQMIGIGGAIGTGLFLGAGGRLAKAGPGLFLVYAVCGVFVFLILR 79 Query: 58 ALSEMTVANPVAGSFRTYTEQAFGRGAGFTVGWVYWTGLVLAMSSEATAVSILLRGW--F 115 AL E+ + P +GSF +Y + FG A + VGW+Y+ + + TA++ L W F Sbjct: 80 ALGELVLHRPSSGSFVSYAREFFGEKAAYVVGWLYFLDWAMTAIVDTTAIATYLHRWTIF 139 Query: 116 PALPLALLGSGIIAGVTLLNLLGAKRLSRLESXXXXXXXXXXXXXXXXXXXXXXXXXXXX 175 ALP L +A V ++NL+ + LE Sbjct: 140 TALPQWTLALLALAVVLVMNLISVEWFGELEFWAALIKVCALMAFLVVGTIFLGGRYPVD 199 Query: 176 XRAVGLDVVRGQS-LFPGGIAGIAGSMLMVMFTYAGFEVLGLAASETGNPGVTIPRAIRY 234 GL + LFP G+A + VMF YA E++G AA ET P +PRAI Sbjct: 200 GHNTGLSLWTSHGGLFPTGVAPLIVVSSGVMFAYAAVELVGTAAGETVEPKKIMPRAINS 259 Query: 235 TIVLLVGLYLAAIIGLFLLLPPARVSEQVSPFVSALSLYGLGWAGTVMNIVLVSAILSTM 294 I + Y+ ++I L LLLP + SPFV+ S G AG +MNIV+++A LS++ Sbjct: 260 VIARIAIFYVGSVILLALLLPYSAFKASESPFVTFFSKVGFYGAGDLMNIVVLTAALSSL 319 Query: 295 LASVFGLGRMLRSLAEEGHTPAWM--RDRADIPYRGILIS 332 A ++ GR++ S+A G P + + +PY GIL++ Sbjct: 320 NAGLYATGRVMHSIAINGSGPKFTARMSKNGVPYGGILLA 359 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,599,983 Number of extensions: 991766 Number of successful extensions: 3393 Number of sequences better than 1.0e-05: 165 Number of HSP's gapped: 3324 Number of HSP's successfully gapped: 257 Length of query: 519 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 408 Effective length of database: 25,576,357 Effective search space: 10435153656 Effective search space used: 10435153656 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)