BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0001.p (358 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0840 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzym... 318 8e-87 CC2129 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzym... 297 2e-80 CC1802 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzym... 296 3e-80 SMa2291 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzy... 152 1e-36 VCA0998 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzy... 109 5e-24 >PA0840 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzyme family Length = 346 Score = 318 bits (815), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 0/358 (0%), Positives = 0/358 (0%), Gaps = 6/358 (1%) Query: 6 FQLLLKQQIRRIVMSPVARAFSPGGVPGEDVTAYYRRRAENEVGLIITEGVVIDHPAASS 65 L AE E + + Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 66 EPRLPGIYGEAAMDGWREVVRQVHEAGGTIFPQLVHLGMIRPMGAEPFPEAPSIGPSGLD 125 G + + + ++ G L + + Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV 131 Query: 126 LEGRQVTGPMTEEEIXXXXXXXXXXXXXXXXXXXDGVELHGAHGFLIDQFFWEKTNQRTD 185 ++ T + + + + Sbjct: 132 HWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLH 191 Query: 186 RYGGDFLKRTXXXXXXXXXXXXXXGPDYPVSMRLSQWKMGDYAAKLVGTPEQLEQFLNVL 245 L R + ++ Y+ GT Sbjct: 192 LADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKP 251 Query: 246 VKAGVDIFHISTRRFWDPEFEGSELSLAGWVKKLSGKTTITVGSVGMEDEP------SED 299 A +F TR + + + + + + V + + Sbjct: 252 AGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATT 311 Query: 300 GEAGRHGXXXXXXXXXXXXFDLIAVGRALLGDPAWAKKIREGRVDEIKAFTPEATAVL 357 Sbjct: 312 369 >CC2129 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzyme family Length = 346 Score = 297 bits (761), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 0/364 (0%), Positives = 0/364 (0%), Gaps = 12/364 (3%) Query: 3 DSFFQLLLKQQIR---RIVMSPVARAFSPGGVPGEDVTAYYRRRAENEVGLIITEGVVID 59 L+ + V E+ + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 60 HPAASSEPRLPGIYGEAAMDGWREVVRQVHEAGGTIFPQLVHLGMIRPMGAEPFPEAPSI 119 L + +V I P Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNI--REALHDSLKR- 120 Query: 120 GPSGLDLEG-RQVTGPMTEEEIXXXXXXXXXXXXXXXXXXXDGVELHGAHGFLIDQFFWE 178 L+ + I Sbjct: 121 -LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 179 KTNQRTDRYGGDFL-KRTXXXXXXXXXXXXXXGPDYPVSMRLSQWKMGDYAAKLVGTPEQ 237 K + V + G Sbjct: 180 NETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 238 LEQFLNVLVKAGVDIFHISTRRFWDPEFEGSELSLAGWVKKLSGKTTITVGSVGMEDE-- 295 + + RF E ++ ++A +V Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 296 PSEDGEAGR-HGXXXXXXXXXXXXFDLIAVGRALLGDPAWAKKIREGRVDEIKAFTPEAT 354 P Sbjct: 300 PFVASTLLGA 359 Query: 355 AVLF 358 Sbjct: 360 363 >CC1802 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzyme family Length = 346 Score = 296 bits (758), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 0/349 (0%), Positives = 0/349 (0%), Gaps = 7/349 (2%) Query: 15 RRIVMSPVARAFSPGGVPGEDVTAYYRRRAENEVGLIITEGVVIDHPAASSEPRLPGIYG 74 I + + D + E + P+ P Sbjct: 82 LIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCF 141 Query: 75 EAAMDGWREVVRQVHEAGGTIFPQLVHLGMIRPMGAEP-FPEAPSIGPSGLDLEGRQVTG 133 + T+ + + A + + + Sbjct: 142 GK-LGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPR 200 Query: 134 PMTEEEIXXXXXXXXXXXXXXXXXXXDGVELHGAHGFLIDQFFWEKTNQRTDRYGGDFLK 193 +T + L + + Sbjct: 201 IVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFS 260 Query: 194 RTXXXXXXXXXXXXXXGPDYPVSMRLSQWKMGDYAAKLVGTPEQLEQFLNVLVKAGVDIF 253 R D L +M + + + +I Sbjct: 261 RFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIE 320 Query: 254 HISTRRFWDPEFEGSELSLAGWVKKLSGKTTITVGSVGMEDEPSEDGEAG-----RHGXX 308 Sbjct: 321 380 Query: 309 XXXXXXXXXXFDLIAVGRALLGDPAWAKKIREGRVDEIKAFTPEATAVL 357 Sbjct: 381 429 >SMa2291 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzyme family Length = 346 Score = 152 bits (383), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 14/333 (4%), Positives = 46/333 (13%), Gaps = 32/333 (9%) Query: 16 RIVMSPVAR-AFSPGGVPGEDVTAYYRRRAENEVGLIITEGVVIDHPAASSEPRLPGIYG 74 A G ++ + + Sbjct: 25 EQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLII 84 Query: 75 EAAMDGWREVVRQVHEAGGTIFPQLVHLGMIRPMGAEPFPEAPSIGPSGLDLEGRQVTG- 133 + + G + + + + + + + + + R Sbjct: 85 ASKVSGPSRNNDKGIRPDQALDRKNIREALHDS--LKRL-QTDYLDLYQVHWPQRPTNCF 141 Query: 134 ----------PMTEEEIXXXXX-XXXXXXXXXXXXXXDGVELHGAHGFLIDQFFWEKTNQ 182 + G +L + Sbjct: 142 GKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRI 201 Query: 183 RTDRYGGDFLKRTXXXXXXXXXXXXXXGPDYPVSMRLSQWKMGDYAAKLVGTPEQLEQFL 242 T + L R+ + Sbjct: 202 VTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSR 261 Query: 243 NVLVKAGVDIFHISTRRFWDPEFEGSELSLAGWVKKLSGKT-TITVGSVGMEDEPSEDGE 301 ++ +A + + +G+ M+ + Sbjct: 262 FTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIES 321 Query: 302 AGRHGXXXXXXXXXXXXFDLIAVGRALLGDPAW 334 +L A + Sbjct: 322 LHL---------------ELSEDVLAEIEAVHQ 339 >VCA0998 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzyme family Length = 346 Score = 109 bits (273), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 10/353 (2%), Positives = 45/353 (12%), Gaps = 51/353 (14%) Query: 15 RRIVMSPVARAFSPGG-VPGEDVTAYYRRRAENEVGLIITEGVVIDHPAASSEPRLPGIY 73 + + + + Sbjct: 19 GTMTFGEQNSEADAHAQLDYAVAQGINLIDVA--EMYPVPPRPET-QGLTETYVGNWLAK 75 Query: 74 GEAAMDGWREVVRQVHEAGGTIFPQLVHLGMIRPMGAEP--FPEAPSIGPSGLDLEGR-- 129 + + L+ Sbjct: 76 HGSREKLIIASKVSGPSRNNDKGIRPDQALDR----KNIREALHDSLKRLQTDYLDLYQV 131 Query: 130 -----QVTGP--MTEEEIXXXXXXXXXXXXXXXXXXXDGVELHGAHGFLIDQFFWEKTNQ 182 + ++ F + Sbjct: 132 HWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLH 191 Query: 183 RTDRYGGDFLKRTXXXXXXXXXXXXXXGPDYPVSMRLSQWKMGDYAAKLVGTPEQLEQFL 242 D++ + + + +G ++L Sbjct: 192 LADKHDLPRIVTIQNPYSLLNRSFEVGLAE-VSQYEGVE---LLA-YSCLGFGTLTGKYL 246 Query: 243 NVLVKA-GVDIFHISTRRFWDP---EFEGSELSLAGWVKKLSGKTTITVGSVGMEDEPS- 297 N A + R+ + + + + + + + V + + Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDI--ARRHGLDPAQMALAFVRRQPFVAS 304 Query: 298 -EDGEAGRHGXXXXXXXXXXXXFDLIAVGRALLGDPAWAKKIREGRVDEIKAF 349 G + + + + + Sbjct: 305 TLLGA-----------------TTMDQLKTNIESLHLELSEDVLA--EIEAVH 338 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,409,128 Number of extensions: 1003659 Number of successful extensions: 1843 Number of sequences better than 1.0e-05: 74 Number of HSP's gapped: 1719 Number of HSP's successfully gapped: 75 Length of query: 358 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 250 Effective length of database: 25,922,359 Effective search space: 6480589750 Effective search space used: 6480589750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)