BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0040.p (493 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all4345 [G] COG0726 Predicted xylanase/chitin deacetylase 136 7e-32 CAC1359 [G] COG0726 Predicted xylanase/chitin deacetylase 136 8e-32 CAC1037 [G] COG0726 Predicted xylanase/chitin deacetylase 127 3e-29 alr1793 [G] COG0726 Predicted xylanase/chitin deacetylase 127 6e-29 BS_yheN [G] COG0726 Predicted xylanase/chitin deacetylase 126 1e-28 >all4345 [G] COG0726 Predicted xylanase/chitin deacetylase Length = 305 Score = 136 bits (343), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 75/188 (39%), Positives = 113/188 (60%), Gaps = 10/188 (5%) Query: 142 QKTVYLTFDDGP-SKITPKVLEILRRQGVKATFFVLGEQAEQRPELISAIWEQGHAIGNH 200 QK + LTFDDGP + T +VL+IL++ +KATFFV+G+ + P+L+ + +GHAIGNH Sbjct: 105 QKVIALTFDDGPWPESTTQVLDILKQNNIKATFFVVGQNVKNYPDLLRRVAAEGHAIGNH 164 Query: 201 TYNHNYRDLYSSFTEFWSQIKQTEEIVRNITGVRPQLVRAPGGTFGHFDKTYFRLLAQAG 260 T++H Y + + ++ T ++ ITGV+ L R PGG + +Y R Sbjct: 165 TWHHWYHFMNPQVAAY--EVNNTTNLIYQITGVKTDLFRPPGGMMNNGLVSYAR---NNK 219 Query: 261 YSVMDWTADSGDSRRKGVPAADILRESVADLTASRVILLLHDGGGHE-QSAKALPEIIKR 319 Y+V+ W++DS D R VP L +V L I+L+HDGGG+ Q+ +ALPEII R Sbjct: 220 YAVIMWSSDSIDYSRPSVPR---LINNVFRLAKPGGIVLMHDGGGNRTQTVQALPEIIAR 276 Query: 320 YKAAGYTF 327 ++ GY+F Sbjct: 277 FRKQGYSF 284 >CAC1359 [G] COG0726 Predicted xylanase/chitin deacetylase Length = 277 Score = 136 bits (342), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 73/224 (32%), Positives = 121/224 (54%), Gaps = 10/224 (4%) Query: 116 RAEAAVKNGAAVSKVPAKP-------TLPAQTKQKTVYLTFDDGPSKITPKVLEILRRQG 168 + E ++K + +P T+ + +K YLTFDDGPS T K++EIL R Sbjct: 55 KKENSIKKDVPKDNIKNQPGGQYTPWTVKREDGKKVAYLTFDDGPSVNTSKIIEILNRNN 114 Query: 169 VKATFFVLGEQAEQRPELISAIWEQGHAIGNHTYNHNYRDLYSSFTEFWSQIKQTEEIVR 228 +KATFF++G+ AE+ P+++ ++ G IGNHTY+H + S+ + + + ++R Sbjct: 115 IKATFFLIGKSAEEFPDMVRQEFQNGEVIGNHTYSHILSNK-SNPEKLIEDFNKCDGVLR 173 Query: 229 NITG--VRPQLVRAPGGTFGHFDKTYFRLLAQAGYSVMDWTADSGDSRRKGVPAADILRE 286 I G +L R PGG+FG + + +AGY +DW + GD+R + +P + +L+ Sbjct: 174 AILGKNYNSRLARFPGGSFGDRLRDTREAVGKAGYRFVDWNDEIGDARGRNLPVSILLQN 233 Query: 287 SVADLTASRVILLLHDGGGHEQSAKALPEIIKRYKAAGYTFGVL 330 ++LL+HD + +ALP+II+ K GYTF L Sbjct: 234 LRKYTYPDTIVLLMHDAAPKTSTVEALPQIIQYLKDRGYTFSTL 277 >CAC1037 [G] COG0726 Predicted xylanase/chitin deacetylase Length = 261 Score = 127 bits (320), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 75/211 (35%), Positives = 119/211 (56%), Gaps = 8/211 (3%) Query: 127 VSKVPAKPTLPAQTK-QKTVYLTFDDGPS-KITPKVLEILRRQGVKATFFVLGEQAEQRP 184 +SK K + TK +KT YLTFDDGPS +TP +L+ L+ VKATFFV G+ + Sbjct: 51 LSKKSIKTKNVSNTKTEKTAYLTFDDGPSSNVTPYILDTLKTYDVKATFFVTGKNSIINK 110 Query: 185 ELISAIWEQGHAIGNHTYNHNYRDLYSSFTEFWSQIKQTEEIVRNITG---VRPQLVRAP 241 +S + G++IG ++++N +YSS TE+ + I + +I+R I G +L+R P Sbjct: 111 LTLSKEAQSGNSIGIKSFDNNITKIYSSPTEYINDINECRDILRAILGDGKFNMKLIRFP 170 Query: 242 GGTFGHFDKTYFRLLAQAGYSVMDWTADSGDSRRKGVPAADILRESVAD--LTASRVILL 299 GG+ K + + + +AGY+ +DW DS D+ R + + SV ++I+L Sbjct: 171 GGS-TMVSKNFKKSIKKAGYNFVDWDIDSKDALRTKQKLPNDIVNSVKSSYKNNDKIIIL 229 Query: 300 LHDGGGHEQSAKALPEIIKRYKAAGYTFGVL 330 +HD +E + +ALPEII+ K+ Y F L Sbjct: 230 MHDSSSNEATLQALPEIIQFLKSQNYVFKTL 260 >alr1793 [G] COG0726 Predicted xylanase/chitin deacetylase Length = 359 Score = 127 bits (318), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 12/207 (5%), Positives = 28/207 (13%), Gaps = 21/207 (10%) Query: 135 TLPAQTKQKTVY------------LTFDDGP-SKITPKVLEILRRQGVKATFFVLGEQAE 181 T+ S P + + G+ + Sbjct: 80 GFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEA 139 Query: 182 QRPELISAIWEQGHAIGNHTYNHNYRDLYSSFTEFWSQIKQTEEIVRNITGVRPQLVRAP 241 L + + + + Sbjct: 140 FYHRLTQCKNVRQPVNIVSHFARADEPKCGATEK--QLAIFNTFCEGKPGQRSIAASGGI 197 Query: 242 GGTFGHFDKTYFRLLAQAGYSVMDWTADSGDSRRKGVPAADILRESVADLTASRVILLLH 301 Y V D G ++ + + + Sbjct: 198 LLWPQSHFDWVRP--GIILYGVS-PLEDRSTGADFGCQ--PVMSLTSSLIAVREHKAGEP 252 Query: 302 DGGGHEQ-SAKALPEIIKRYKAAGYTF 327 G G S + + Sbjct: 253 VGYGGTWVSERDTRLGVVAMGYGDGYP 279 >BS_yheN [G] COG0726 Predicted xylanase/chitin deacetylase Length = 128 Score = 126 bits (316), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 0/208 (0%), Positives = 0/208 (0%), Gaps = 11/208 (5%) Query: 139 QTKQ----KTVYLTFDDGPSKITPKVLEILRRQGVKATFFVLGEQAEQRPELISAIWEQG 194 Sbjct: 77 ICVAAALR 136 Query: 195 HAIGNHTYNHNYRDLYSSFTEFWSQIKQTEEIVRNITGVRPQLVRAPGGTFGHFDKTYFR 254 Sbjct: 137 196 Query: 255 LLAQAGYSVMDWTADSGDSRRKG---VPAADILRESVADLTASR-VILLLHDGGGHEQSA 310 Sbjct: 197 --- 253 Query: 311 KALPEIIKRYKAAGYTFGVLDGEVQPVQ 338 Sbjct: 254 281 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,593,209 Number of extensions: 1447195 Number of successful extensions: 4745 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 4690 Number of HSP's successfully gapped: 40 Length of query: 493 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 383 Effective length of database: 25,691,691 Effective search space: 9839917653 Effective search space used: 9839917653 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)