BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0039.p (223 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2057 [S] COG0586 Uncharacterized membrane-associated protein 129 2e-30 BS_ybfM [S] COG0586 Uncharacterized membrane-associated protein 120 1e-27 lin1535 [S] COG0586 Uncharacterized membrane-associated protein 87 3e-17 BS_yngC [S] COG0586 Uncharacterized membrane-associated protein 86 5e-17 TVN0651 [S] COG0586 Uncharacterized membrane-associated protein 85 1e-16 >BH2057 [S] COG0586 Uncharacterized membrane-associated protein Length = 200 Score = 129 bits (325), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 60/143 (41%), Positives = 92/143 (64%), Gaps = 4/143 (2%) Query: 31 PFPGETTMAFAGFLSYTGKLDFFVLIAVAFAGTTVGMTVTYFIGLKAGLPFIQRYGKWFL 90 P P E + G+ GK+ + + + +F G T+G++++YF+G K GLPF+ +YG F Sbjct: 28 PIPDEVLLTLVGYYISLGKMSYVLALLASFLGATLGISISYFLGTKLGLPFLMKYGPKFH 87 Query: 91 FSETKLEKTQHWFDRYGSILVSIGYFIPGVRHFTGYFSGITALPFRKFALYAYSGALLWV 150 S K++ Q F +YG IL+ IGYF+PGVRH TGY SG++ FR F+LYAY+GA +W Sbjct: 88 ISHQKIDHAQALFRKYGPILLFIGYFLPGVRHVTGYLSGLSKCKFRTFSLYAYTGAFIWC 147 Query: 151 VLFLGIGKIFGPQWMGIFHLVEK 173 ++F+ +G G +W ++VEK Sbjct: 148 LVFITLGHEIGNKW----YIVEK 166 >BS_ybfM [S] COG0586 Uncharacterized membrane-associated protein Length = 468 Score = 120 bits (302), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 13/158 (8%), Positives = 26/158 (16%) Query: 4 LKWIQELFAXXXXXXXXXXXXXXXXXXPFPGETTMAFAGFLSYTGKLDFFVLIAVAFAGT 63 ++ + P L+ Sbjct: 1 MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGGIYYWQRERDRKDVTDG 60 Query: 64 TVGMTVTYFIGLKAGLPFIQRYGKWFLFSETKLEKTQHWFDRYGSILVSIGYFIPGVRHF 123 T A L F Y + + S + F + + Sbjct: 61 DKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAENGDSSHPNEIAFSKSNKAY 120 Query: 124 TGYFSGITALPFRKFALYAYSGALLWVVLFLGIGKIFG 161 +SG + A + +W Sbjct: 121 DEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQT 158 >lin1535 [S] COG0586 Uncharacterized membrane-associated protein Length = 208 Score = 86.7 bits (213), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 46/143 (32%), Positives = 72/143 (50%), Gaps = 4/143 (2%) Query: 31 PFPGETTMAFAGFLSYTGKLDFFVLIAVAFAGTTVGMTVTYFIGLKAGLPFIQRYGKWFL 90 P P E M F GF++ GKL+F++++ AG VG + Y+IG G + ++GK+ Sbjct: 37 PLPSEVIMLFGGFMAEAGKLNFWLVVFAGIAGNLVGSLIAYYIGKFGGRALVLKFGKYIF 96 Query: 91 FSETKLEKTQHWFDRYGSILVSIGYFIPGVRHFTGYFSGITALPFRKFALYAYSGALLWV 150 + L+K + WF RYG+ V G +P +R F +GI + KF +Y G + W Sbjct: 97 LNVKHLDKAELWFGRYGARAVFFGRVLPVIRTFISLPAGIAKMNVWKFVIYTILGCIPWN 156 Query: 151 VLFLGIGKIFGPQWMGIFHLVEK 173 + +G G W +VEK Sbjct: 157 IFLTWLGYSLGSNW----SVVEK 175 >BS_yngC [S] COG0586 Uncharacterized membrane-associated protein Length = 198 Score = 85.9 bits (211), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 43/133 (32%), Positives = 69/133 (51%) Query: 32 FPGETTMAFAGFLSYTGKLDFFVLIAVAFAGTTVGMTVTYFIGLKAGLPFIQRYGKWFLF 91 P E +A+ G++ G + F I G T+ Y+IG G PF+ +YGK+ L Sbjct: 29 IPSEIVLAYGGYMVSEGTIGFIGAIIAGVIGGTIAQIFIYWIGRYGGRPFLDKYGKYLLI 88 Query: 92 SETKLEKTQHWFDRYGSILVSIGYFIPGVRHFTGYFSGITALPFRKFALYAYSGALLWVV 151 + ++ +++WF +YG+ +V FIP VRH +GI +PF KF + + W + Sbjct: 89 KKHHIDMSENWFQKYGAGVVFSARFIPVVRHAISIPAGIARMPFLKFVVLTVLAIIPWSI 148 Query: 152 LFLGIGKIFGPQW 164 LF+ +G G QW Sbjct: 149 LFVYLGIQLGSQW 161 >TVN0651 [S] COG0586 Uncharacterized membrane-associated protein Length = 261 Score = 84.7 bits (208), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 44/134 (32%), Positives = 67/134 (50%) Query: 31 PFPGETTMAFAGFLSYTGKLDFFVLIAVAFAGTTVGMTVTYFIGLKAGLPFIQRYGKWFL 90 P P E + +GFL Y G +F V++A + VG + YF+GL G P I + +F Sbjct: 85 PIPSEVILPLSGFLVYQGSFNFAVVLADTIIASMVGSFIDYFLGLYLGRPIIVKILSYFS 144 Query: 91 FSETKLEKTQHWFDRYGSILVSIGYFIPGVRHFTGYFSGITALPFRKFALYAYSGALLWV 150 SE L + + W D+ GS V + FIPG+R +G+ + FAL + G+ +W Sbjct: 145 ISEDSLSRAETWIDKMGSFSVFLARFIPGIRSLISLPAGMLKMKMWLFALMTFLGSFIWS 204 Query: 151 VLFLGIGKIFGPQW 164 + +G GP W Sbjct: 205 FSLIYLGYSAGPYW 218 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.146 0.464 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,296,203 Number of extensions: 538772 Number of successful extensions: 1306 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 1313 Number of HSP's successfully gapped: 50 Length of query: 223 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 121 Effective length of database: 26,614,363 Effective search space: 3220337923 Effective search space used: 3220337923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 114 (48.5 bits)