BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0038.p (429 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa1641 [GEPR] COG0477 Permeases of the major facilitator superf... 60 7e-09 >SMa1641 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 440 Score = 60.1 bits (144), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 36/127 (28%), Positives = 74/127 (58%), Gaps = 7/127 (5%) Query: 12 EVLKRRSKGYSRLFAAGLVNGIGDRFSSVAM--LALILQVTGSGMAVGISLGMRVLPFLF 69 +VL R+ Y RLF A ++ +G ++VA+ LA L +G +G +L ++++ ++ Sbjct: 3 QVLANRT--YRRLFLAQVIALVGTGLATVALGLLAFDLAGADAGAVLGTALAIKMIAYVG 60 Query: 70 MAPLGGMLAGRLPRKTIMMAVDLLRVPVALSFLLVDGAGRLWLLYAGSFIMAAGEALYSP 129 +AP+ A +LPR+++++ +DL+R VA+ V +W +Y F++ + A ++P Sbjct: 61 VAPVAAAFAEQLPRRSMLVCLDLVRAAVAVFLPFVT---EVWQVYVLIFVLQSASAAFTP 117 Query: 130 VRKSSIP 136 +++IP Sbjct: 118 TFQATIP 124 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.141 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,105,668 Number of extensions: 918987 Number of successful extensions: 3317 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 3323 Number of HSP's successfully gapped: 11 Length of query: 429 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 320 Effective length of database: 25,807,025 Effective search space: 8258248000 Effective search space used: 8258248000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)