BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0036.p (480 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 438 e-123 lin0519 [GEPR] COG0477 Permeases of the major facilitator superf... 350 3e-96 BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superf... 349 7e-96 L132251 [GEPR] COG0477 Permeases of the major facilitator superf... 262 1e-69 BH0554 [GEPR] COG0477 Permeases of the major facilitator superfa... 191 3e-48 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 438 bits (1127), Expect = e-123, Method: Compositional matrix adjust. Identities = 213/468 (45%), Positives = 316/468 (67%), Gaps = 4/468 (0%) Query: 1 VALLNQTLMNVALPKMMEDLKIAANTAQWLTTGFMLVNGVLIPISAYLVEKFTTRQLFTT 60 V +LNQTLMNVALP +M+D I A+ QWL+TGFMLVNGV+IP++A+L+E+FTTRQL+ Sbjct: 23 VTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNGVMIPMTAFLIERFTTRQLYLF 82 Query: 61 AMMLFSIGTLVCAIGTGFEMIMVGRVIQAVGAGILMPLMNIVFLRIFPIEERGKAMGLMA 120 AM+ F+IGT + T + M++ GR++QA+GAGI+MPL+ +V L +FP+E RG+AMGL+ Sbjct: 83 AMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLLTVVVLNLFPMERRGRAMGLIG 142 Query: 121 VAMIFAPAVGPTLSGWVVQNYSWRVLFYIVLPLAVFSTLLGMKTMQNVGKLTSPRLDKMG 180 +AM FAPA+GPTLSGW+VQ Y WR LF+I++P A+ ++ + ++NVGK T P+LD +G Sbjct: 143 LAMNFAPAIGPTLSGWIVQQYDWRNLFFIIIPFAILDIVVAIFLLKNVGKRTFPKLDILG 202 Query: 181 VVLSTLAFGGLLYGFSDAGTDGWNSATVYACLILGVVALALFVWRELKTEKPLLEFRIFR 240 V++ST+ FG LL GFS+AG W + V +ILG+V L +F+ + + PLL F++F+ Sbjct: 203 VIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGLVVLGIFIRYQSSNKAPLLNFKVFK 262 Query: 241 YNMYSLTTVINIIVTMALYSGMILLPIYLQTIRGFTPMESGLMLLPGAILMGVMSPITGI 300 Y ++LTT I+ V M L+ GM+LLPI+LQT+RGF+P+ESGL+LLPGA++ ++SP+TG+ Sbjct: 263 YPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLESGLVLLPGALVTAILSPVTGV 322 Query: 301 IFDKIGARWLSVIGLIITTITTWEFSQXXXXXXXXXXXXXXXARMFGMSMLMMPIVTAGL 360 +FD+ GA++LS++GLII +T+ F+ R GM+M+MMP+ TA L Sbjct: 323 MFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTFIIIVQTIRSAGMAMVMMPLQTAAL 382 Query: 361 NQLPQRLASHGTAMSNTLRTVGGALGMALFVSLMTNRTKSDITEAVMSGSVSQTDKAAML 420 N LP +LASHG+AM NT+R V G++G A +++M+ S + + + +T Sbjct: 383 NSLPLKLASHGSAMFNTMRQVAGSIGTAALITVMSKSAASFADKLGPADVIGKTKT---- 438 Query: 421 KLTQDATINGITHAFXXXXXXXXXXXXXXXFIKKTSPQPDFLQTEDTE 468 ++ I+GI AF FI+K D + T+ E Sbjct: 439 EVANHVLIHGIETAFLVAGILSVVACILALFIQKNRSAMDPIVTKTEE 486 >lin0519 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 471 Score = 350 bits (898), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 184/434 (42%), Positives = 277/434 (63%), Gaps = 2/434 (0%) Query: 1 VALLNQTLMNVALPKMMEDLKIAANTAQWLTTGFMLVNGVLIPISAYLVEKFTTRQLFTT 60 + L ++T +N+AL +++ I++ T QWLTTG++L G+L+PIS L++ FTTR LF T Sbjct: 26 IGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVPISGLLLQWFTTRGLFFT 85 Query: 61 AMMLFSIGTLVCAIGTGFEMIMVGRVIQAVGAGILMPLMNIVFLRIFPIEERGKAMGLMA 120 A+ GTL+ A+ F M+M+GRV+QAVG +L+PLM L IFP +RG AMG++ Sbjct: 86 AVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLPLMFNTILLIFPEHKRGSAMGMIG 145 Query: 121 VAMIFAPAVGPTLSGWVVQNYSWRVLFYIVLPLAVFSTLLGMKTMQNVGKLTSPRLDKMG 180 + ++FAPAVGPT+SG +++N +W +F+I LP + + L GMK MQNV +T P++D + Sbjct: 146 LVIMFAPAVGPTISGLILENLTWNWIFWISLPFLIIALLFGMKFMQNVSVVTKPKIDILS 205 Query: 181 VVLSTLAFGGLLYGFSDAGTDGWNSATVYACLILGVVALALFVWRELKTEKPLLEFRIFR 240 ++LSTL FGG+++ FS AG +GW SATV +I+G +AL LFVWR+L EKPL++ ++F+ Sbjct: 206 IILSTLGFGGVVFAFSSAGENGWGSATVLVSIIVGGIALGLFVWRQLTMEKPLMDLKVFK 265 Query: 241 YNMYSLTTVINIIVTMALYSGMILLPIYLQTIRGFTPMESGLMLLPGAILMGVMSPITGI 300 Y M++L ++ I M + S MILLP+YLQ +GL+LLPG +L G+MSP TG Sbjct: 266 YPMFTLGLILVFISFMMILSTMILLPLYLQNSLALAAFSAGLVLLPGGVLNGLMSPFTGR 325 Query: 301 IFDKIGARWLSVIGLIITTITTWEFSQXXXXXXXXXXXXXXXARMFGMSMLMMPIVTAGL 360 +FD G R L + G I+ I + ++ M G+SM+MMP T GL Sbjct: 326 LFDAYGPRALVIPGFIVAVIALFFLTRIEVGTSALTIIVLHSVLMIGISMVMMPAQTNGL 385 Query: 361 NQLPQRLASHGTAMSNTLRTVGGALGMALFVSLMTNRTKSDITEAVMSGSVSQTDKAAML 420 NQLP +L GTA+ NTL+ V GA+G A+ +++M+ K+ + A G + A++ Sbjct: 386 NQLPPKLYPDGTAIMNTLQQVSGAIGTAVAITIMSAGQKAYMETA--QGVGPEQMIASLT 443 Query: 421 KLTQDATINGITHA 434 Q+A + G+ A Sbjct: 444 AGIQNAFVFGLIMA 457 >BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 479 Score = 349 bits (895), Expect = 7e-96, Method: Compositional matrix adjust. Identities = 184/456 (40%), Positives = 275/456 (60%), Gaps = 11/456 (2%) Query: 1 VALLNQTLMNVALPKMMEDLKIAANTAQWLTTGFMLVNGVLIPISAYLVEKFTTRQLFTT 60 + + ++T +N+AL +M++L I A T QWLTTG++LV G+L+P+S L++ FTTRQLFT Sbjct: 28 IGMFSETALNIALTDLMKELNITAATVQWLTTGYLLVLGILVPVSGLLLQWFTTRQLFTV 87 Query: 61 AMMLFSIGTLVCAIGTGFEMIMVGRVIQAVGAGILMPLMNIVFLRIFPIEERGKAMGLMA 120 +++ +GT + A+ F ++ R++QA+G G+L+PLM L IFP +RG AMG + Sbjct: 88 SLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNTILVIFPPHKRGAAMGTIG 147 Query: 121 VAMIFAPAVGPTLSGWVVQNYSWRVLFYIVLPLAVFSTLLGMKTMQNVGKLTSPRLDKMG 180 + ++FAPA+GPT SG V+++ +W +F+I LP V + + G+ MQNV + T P++D + Sbjct: 148 LVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVLALVFGIAYMQNVSETTKPKIDVLS 207 Query: 181 VVLSTLAFGGLLYGFSDA--GTDGWNSATVYACLILGVVALALFVWRELKTEKPLLEFRI 238 ++LST+ FGG+++GFS+A G+ GW+S TV LI+GVV L LF R+L ++P++ R Sbjct: 208 IILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVSLIVGVVGLILFSIRQLTMKQPMMNLRA 267 Query: 239 FRYNMYSLTTVINIIVTMALYSGMILLPIYLQTIRGFTPMESGLMLLPGAILMGVMSPIT 298 F+Y M+ L ++ I M + S M+LLP+YLQ T SGL+LLPG IL G MSP+T Sbjct: 268 FKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFASGLVLLPGGILNGFMSPVT 327 Query: 299 GIIFDKIGARWLSVIGLIITTITTWEFSQXXXXXXXXXXXXXXXARMFGMSMLMMPIVTA 358 G +FDK G +WL + G +I T+ W FS M G+SM+MMP T Sbjct: 328 GRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIILHTCLMIGISMIMMPAQTN 387 Query: 359 GLNQLPQRLASHGTAMSNTLRTVGGALGMALFVSLMTNRTKSDITEAVMSGSVSQTDKAA 418 GLNQLP+ GTA+ NTL+ + GA+G A+ VS+M MS + D A Sbjct: 388 GLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAVSIMAAGQHD-----YMSTVKNPADPAV 442 Query: 419 MLKLTQDATINGITHAFXXXXXXXXXXXXXXXFIKK 454 + A G+ HAF F+K+ Sbjct: 443 I----PQALTAGVQHAFVFAMIVAIIGLIGAFFMKR 474 >L132251 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 514 Score = 262 bits (669), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 152/447 (34%), Positives = 252/447 (56%), Gaps = 17/447 (3%) Query: 2 ALLNQTLMNVALPKMMEDLKIAANTAQWLTTGFMLVNGVLIPISAYLVEKFTTRQLFTTA 61 A+L QT + A+P +M D I+ +TAQ TT F+L NG+++PISAYL KF TR L+ TA Sbjct: 34 AMLMQTSLGTAIPTLMNDFNISMSTAQQATTWFLLANGIMVPISAYLATKFPTRWLYVTA 93 Query: 62 MMLFSIGTLVC--AIGTGFEMIMVGRVIQAVGAGILMPLMNIVFLRIFPIEERGKAMGLM 119 IG V A + + + + GR++QA+ GI MPLM + + +FP ++ G MG+ Sbjct: 94 YATLFIGMFVAYSAPTSSWWVFLAGRILQAIAVGITMPLMQVALVNMFPAKQMGAIMGVA 153 Query: 120 AVAMIFAPAVGPTLSGWVVQNY----------SWRVLFYIVLPLAVFSTLLGMKTMQNVG 169 V ++ APA+GPT +GW++ + SWR +F I + + L+ + M++V Sbjct: 154 GVVLVLAPALGPTFAGWLITHKFNLIFWTFAASWRTIFLIPMIVVGVVFLISIFAMRDVI 213 Query: 170 KLTSPRLDKMGVVLSTLAFGGLLYGFSDAGTDGWNS-ATVYACLILGVVALALFVWRELK 228 +LD + V+LS + FG L+GF++ TDGW TV A ++ G++ + LF R+LK Sbjct: 214 PNRPMKLDFLSVLLSVVGFGAFLWGFTNVATDGWGKFNTVIAPIVGGLIIIFLFGLRQLK 273 Query: 229 TEKPLLEFRIFRYNMYSLTTVINIIVTMALYSGMILLPIYLQTIRGFTPMESGLMLLPGA 288 + P L+ R+F+ ++L T+ + TMA+ ++LP+YLQ + G + SGL+LLPG+ Sbjct: 274 MDDPFLDIRVFKIRQFTLVTLALALSTMAMMGVEMMLPLYLQDVHGLSAFNSGLVLLPGS 333 Query: 289 ILMGVMSPITGIIFDKIGARWLSVIGLIITTITTWEFSQXXXXXXXXXXXXXXXARMFGM 348 ++M V++P+ G ++D+IG++ L+VIG ++ + T F RM G+ Sbjct: 334 LMMAVVAPLAGGVYDRIGSKRLAVIGFLVLALGTIPFMFFTATTPDHFDTLLYGLRMVGV 393 Query: 349 SMLMMPIVTAGLNQLPQRLASHGTAMSNTLRTVGGALGMALFVSLMTNRTKSDITEAVMS 408 M+MMP+ T+ +N +P + TA +NT R V ++ +AL S+ N IT + + Sbjct: 394 GMVMMPLTTSAMNAVPVNEVAQATASNNTARQVASSVVVALLSSVAQNV----ITNNMPA 449 Query: 409 GSVSQTDKAAMLKLTQDATINGITHAF 435 S+ T+ +A + G +F Sbjct: 450 SSLKSTNPLQFANKAIEAMLQGYHASF 476 >BH0554 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 474 Score = 191 bits (484), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 126/471 (26%), Positives = 230/471 (48%), Gaps = 26/471 (5%) Query: 3 LLNQTLMNVALPKMMEDLKIAANTAQWLTTGFMLVNGVLIPISAYLVEKFTTRQLFTTAM 62 +LN +++N A+P++M + A W+ T FM+ G+ +P++ YL +K ++ + + Sbjct: 22 ILNNSMLNPAIPQLMNVFEADAVATGWVITIFMVAMGMTMPLTGYLGDKIGKKEAYILGL 81 Query: 63 MLFSIGTLVCAIGTGFEMIMVGRVIQAVGAGILMPLMNIVFLRIFPIEERGKAMGLMAVA 122 +F +G+L+ A+ ++V R +Q +G G++MPL + FP ERG A G+ VA Sbjct: 82 GIFVLGSLLGALSWNLPSLIVFRGLQGIGGGVMMPLSMTLIFDAFPRNERGLATGVWGVA 141 Query: 123 MIFAPAVGPTLSGWVVQNYSWRVLFYIVLPLAVFSTLLGMKTMQNVGKLTSPRLDKMGVV 182 + AP +GPTL G++V+ +W+ LF + +P + + + + + + +LD+ G + Sbjct: 142 SMMAPTIGPTLGGFIVETSNWKYLFLVNIPFGLLGIIAAVIYLPKIARSGQIKLDRWGFL 201 Query: 183 LSTLAFGGLLYGFSDAGTDGWNSATVYACLILGVVALA------LFVWRELKTEKPLLEF 236 T G +L F + T I GV+ L +FV E + E+PLL+ Sbjct: 202 FVTAGVGSILLAFGRM-----SDLTHLTEWINGVLLLLGALCLYVFVQVEKRAEQPLLDL 256 Query: 237 RIFRYNMYSLTTVINIIVTMALYSGMILLPIYLQTIRGFTPMESGLMLLPGAILMGVMSP 296 +FR YSL+ + I ++ L++G+ L+P+ LQ + + P+ +GL+ LP A+ G+ Sbjct: 257 SLFRIPAYSLSIWVAGISSIGLFAGIFLVPLLLQQVYDYGPIMTGLVFLPSALFTGLTMS 316 Query: 297 ITGIIFDKIGARWLSVIGLIITTITTWEFSQXXXXXXXXXXXXXXXARMFGMSMLMMPIV 356 I G + DK G + G++I + T+ R GM + MP Sbjct: 317 IGGRMLDKRGPSGIMTAGMMIAAVGTFALGYLHLETGLWYIFAWMAIRGVGMGLTTMPAT 376 Query: 357 TAGLNQLPQRLASHGTAMSNTLRTVGGALGMALFVSLMTNRTKSDITEAVMSGSVSQTDK 416 T G+N +P+ L S G+AM+N LR + A G+ +F+S+ + + A++S S Sbjct: 377 TTGMNAIPEGLISRGSAMNNVLRQMSSAFGI-VFISVFFEVRRGQL--ALVSTSF----- 428 Query: 417 AAMLKLTQDATINGITHAFXXXXXXXXXXXXXXXFIKKTSPQPDFLQTEDT 467 ++AT+ I F +++K + Q + + T Sbjct: 429 -------EEATLQAINEGFFIVGFLTALSIPAAYWLEKKAKQGEQERVNST 472 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,115,168 Number of extensions: 1230606 Number of successful extensions: 5023 Number of sequences better than 1.0e-05: 97 Number of HSP's gapped: 4928 Number of HSP's successfully gapped: 97 Length of query: 480 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 370 Effective length of database: 25,691,691 Effective search space: 9505925670 Effective search space used: 9505925670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 118 (50.1 bits)