BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0033.p (1447 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2401_1 [I] COG1924 Activator of 2-hydroxyglutaryl-CoA dehydra... 779 0.0 FN1139_1 [I] COG1924 Activator of 2-hydroxyglutaryl-CoA dehydrat... 640 0.0 CAC2401_2 [S] COG3580 Uncharacterized protein conserved in bacteria 499 e-140 CAC2401_3 [S] COG3581 Uncharacterized protein conserved in bacteria 323 2e-87 CAC2428 [I] COG1924 Activator of 2-hydroxyglutaryl-CoA dehydrata... 132 3e-30 >CAC2401_1 [I] COG1924 Activator of 2-hydroxyglutaryl-CoA dehydratase (HSP70-class ATPase domain) Length = 553 Score = 779 bits (2011), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/660 (0%), Positives = 0/660 (0%), Gaps = 3/660 (0%) Query: 20 LRIGLDVGSTTAKLVVME-HDVIVYQDYVRHFSDIKKAALSLLSDVRDRFPDREAALTVX 78 +R+ + +++ ++ + H + L + + L Sbjct: 5 IRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTR 64 Query: 79 XXXXXXXXXXXEVPFVQEVIACTKAISELIPQCDTAIELGGEDAKIIYL-SGGIEQRMNT 137 Q+ A + + A + + L R Sbjct: 65 INLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNID 124 Query: 138 ACAGGTGAFIDQMASLLQTDPAGLNVLAEQHERIYPIASRCGVFAKSDVQPLLNEGARRE 197 + ++ L G + +A S + + Sbjct: 125 EKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNA 184 Query: 198 DVAASIFQSIVNQTISGLACGRPIRGRVAFLGGPLTFLPALRERFAETLGLSDSDVLFPE 257 D + + + + + R L+++ + Sbjct: 185 DDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGR 244 Query: 258 HSQYFVAIGSALSQTDPLVLPLSGWMARLDAVDFSADRAEDAELQPLFASPEALEQFRLR 317 +A + Q + + Sbjct: 245 SEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYA-GTREIAAGNTDLSSRTEQQASALEET 303 Query: 318 HSRAAAPRSELDTYQGPCYLGIDAGSTTTKLVITGAADEILYTFYGSNKGNPLQSVSDAL 377 + + + + + G + + ++D + Sbjct: 304 AASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADII 363 Query: 378 KEIYRILPEGCHIAGAYATGYGEGLVKAALRTDGGEVETVAHYKAASKFMPEVDFILDIG 437 I I + +A A + ++A I D Sbjct: 364 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSV 423 Query: 438 GQDMKCIKIRGGAIDSLMLNEACSAGCGSFLESFASALGLGIGEFAAAALESAQPVNLGS 497 + + A +++ + ASA AL ++ + Sbjct: 424 SRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVALAVSEMDRVTQ 483 Query: 498 RCTVFMNSKVKQVQKEGATLADLSAGLAYSVIKNALQKVIKIRNADDLGRNIIVQGGTFY 557 + + + L+ ++ + + + Q Sbjct: 484 QNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRI 543 Query: 558 NEAVLRAFELLTGRTVVRPDIAGVMGAYGCALLAREHAGFDGVSTLLGPEELEAFKYEVS 617 Sbjct: 544 603 Query: 618 PGRCRLCANNCALTISRFPDKSFHVTGNRCERGAGGKKEKNNLPNLMQYKYERFFDYEGL 677 Sbjct: 604 663 >FN1139_1 [I] COG1924 Activator of 2-hydroxyglutaryl-CoA dehydratase (HSP70-class ATPase domain) Length = 553 Score = 640 bits (1652), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/653 (0%), Positives = 0/653 (0%), Gaps = 22/653 (3%) Query: 18 MMLRIGLDVGSTTAKLVVM-EHDVIVYQDYVRHFSDIKKAALSLLSDVRDRFPDREAALT 76 M+ RI + V + + K +S + L Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 77 VXXXXXXXXXXXXEVPFVQEVIACTKAISELIPQCDTAIELGGEDAKIIYLSGGIEQRMN 136 + + + K + A + Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 137 TACAGGTGAFIDQMASLLQTDPAGLNVLAEQHERIYPIASRCGVFAKSDVQPLLNEGARR 196 + + L+ G + FA+ + Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 197 EDVAASIFQSIVNQTISGLACGRPIRGRVAFLGGPLTFLPALRERFAETLGLSDSDVLFP 256 D A + + L + + L A + + Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLT 240 Query: 257 EHSQYFVAIGSALS--------QTDPLVLPLSGWMARLDAVDFSADRAEDAELQPLFASP 308 + + + T V S + + Sbjct: 241 IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYA---------GTREIA-AGNTDLS 290 Query: 309 EALEQFRLRHSRAAAPRSELDTYQGPCYLGIDAGSTTTKLVITGAADEILYTFYGSNKGN 368 EQ AA +L S + A + Sbjct: 291 SRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMH 350 Query: 369 PLQSVSDALKEIYRILPEGCHIAGAYATGYGEGLVKAALRTDGGEVETVAHYKAASKFMP 428 + S + +I ++ A +A + G V AS+ Sbjct: 351 EIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQ 410 Query: 429 EVDFILDIGGQDMKCIKIRGGAIDSLMLNEACSAGCGSFLESFASALGLGIGEFAAAALE 488 I + + + ++S + + + E + + Sbjct: 411 AAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQ 470 Query: 489 SAQPVNLGSRCTVFMNSKVKQVQKEGATLADLSAGLAYSVIKNALQKVIKIRNADDLGRN 548 A V+ R T S V++ A L + ++ L +V L R Sbjct: 471 VALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRP 530 Query: 549 IIVQGGTFYNEAVLRAFELLTGRTVVRPDIAGVMGAYGCALLAREHAGFDGVSTLLGPEE 608 Sbjct: 531 -- 588 Query: 609 LEAFKYEVSPGRCRLCANNCALTISRFPDKSFHVTGNRCERGAGGKKEKNNLP 661 Sbjct: 589 - 640 >CAC2401_2 [S] COG3580 Uncharacterized protein conserved in bacteria Length = 429 Score = 499 bits (1284), Expect = e-140, Method: Compositional matrix adjust. Identities = 231/427 (54%), Positives = 300/427 (70%), Gaps = 2/427 (0%) Query: 680 EQAERGTVGIPRTMNMFENYPFWHTFFTTLRYRTVLSPKSSKKLYESGMDTIPSESICYP 739 E+A+RG VGIPR +NM+ENYPFWHTFFT L +R LSP+SSKK+YE GMDTIPSES+CYP Sbjct: 3 EEAKRGIVGIPRVLNMYENYPFWHTFFTELGFRVELSPRSSKKVYEKGMDTIPSESVCYP 62 Query: 740 AKMAHGHVQSLLGKGVDFIFYPAVVYEKKEDDAAQNHFNCPVVASYPEVIRNNMDGLKEK 799 AK+ HGH+ SL+ +G+ FIFYP V YEKKE + A NH+NCP+V SY EVIRNN+DGL++ Sbjct: 63 AKITHGHIVSLMERGIKFIFYPCVPYEKKEQEGASNHYNCPIVTSYAEVIRNNIDGLRDG 122 Query: 800 NIPLVSPFLTFDDIPALTRVLVKTF--MDIPKEEIAAAVQTGLAEAERAKADLRNKGEET 857 +I +PF++ DD L L++ ++ K E+ AV E E + D+R KGEE Sbjct: 123 DILFKNPFVSLDDKEKLKSRLLEELQEFNLTKVEVNEAVNKAWQEQENLRQDIRKKGEEV 182 Query: 858 LEFLTHSGTKGILLCGHPYHADPEINHGIAEMITGMGLAVLTEDSICHLDRSEGEVGVVN 917 LE+L +G KGI+L G PYH DPEINHGI E+I +AVLTEDSI HL E + +V+ Sbjct: 183 LEYLKKTGKKGIVLSGRPYHIDPEINHGIPELINSFDMAVLTEDSIAHLGNVERPLRIVD 242 Query: 918 QWTYHARMYRAARLAASRNDLELVQLTSFGCGIDAITCDAVQEILERNNKVYTLIKIDEI 977 QW YH+R+Y AA A ++LELVQL SFGCG+DA+T D V+EIL++ K+YTLIKIDE Sbjct: 243 QWVYHSRLYAAASFIAREDNLELVQLNSFGCGLDAVTTDQVEEILDQYGKIYTLIKIDEG 302 Query: 978 SNXXXXXXXXXXXXXXXXEREKGDVKPQLLYKTQANMPFTKEMKETYTILAPQMSPIHFE 1037 SN EREK VKP+ ++ + FTKEMK+ +TIL PQM+P+HF+ Sbjct: 303 SNLGAIRIRIRSLKAAMGEREKNGVKPERKFENPQRIVFTKEMKKKHTILMPQMAPMHFQ 362 Query: 1038 LFERVFRDAGYRLKILESTGPKETEEGLRYVNNDACYPAIVTIGQMLSALKSGDYDPDRT 1097 E + GY L ++ S K +EGL++VNNDACYP+I+ +GQML ALKSG YD + T Sbjct: 363 FLEMALKSEGYNLVVMPSVDKKCVDEGLKHVNNDACYPSIIVVGQMLEALKSGKYDLNNT 422 Query: 1098 AVIMSQT 1104 +VI+SQT Sbjct: 423 SVIISQT 429 >CAC2401_3 [S] COG3581 Uncharacterized protein conserved in bacteria Length = 339 Score = 323 bits (827), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 153/311 (49%), Positives = 204/311 (65%) Query: 1105 GGGCRATNYISLLRKALKDSGLGQIPVISLNASGMENQPGFRISLRLANRLIAAACYGDL 1164 GGGCRATNYI +RKALKD+G G +PVI+++A E PG + + I A YGDL Sbjct: 1 GGGCRATNYIGFIRKALKDAGYGNVPVIAISAQKFEKNPGITYGVSTGIKAIKAVLYGDL 60 Query: 1165 MMRLLHRFRPYEVIPGSAQALFRKGMDRCKSSLSTFSFREYKRLTREIVAEFVQLPVIKT 1224 +MR+L + RPYE + GSA L+ K +D CK + +K+ R+IV EF +L + Sbjct: 61 LMRVLFKVRPYEKVEGSANKLYEKWVDICKKDSQNKKIKTFKKNVRDIVREFDELEINDV 120 Query: 1225 VKPQVGIVGEILIKFHPDANNHXXXXXXXXXXXXVMPDFLDFIFYCVYNPIYKAEQFGKS 1284 KP+VG+VGEIL+KFHP ANN+ VMPD +DF+ Y YN +K + Sbjct: 121 KKPKVGLVGEILVKFHPTANNNVVDVIEKEGAEAVMPDLMDFMLYSSYNATFKDRYLHNN 180 Query: 1285 KKLGFFNPMLISYLEIYRKPVKAALEEAGLAKGRENIYGLAEKASRLVSVGNQMGEGWFL 1344 KK + +LI +E +RKP+K AL E+ + ++I LA+ S +VS+GNQ GEGWFL Sbjct: 181 KKSKILSNVLIDAIEYFRKPMKKALHESKRFEPPKSIKELADGVSDIVSLGNQTGEGWFL 240 Query: 1345 TAEMMDLLDNGVNNIACIQPFACLPNHITGRGMIKGLKDLYPGANIAAIDYDAGVSVVNQ 1404 TAEM++L++ G N+ C+QPFACLPNH+TG+GMIK LK YP ANIAA+D+D G S VNQ Sbjct: 241 TAEMVELIEGGTENVICMQPFACLPNHVTGKGMIKELKRRYPKANIAAVDFDPGASEVNQ 300 Query: 1405 ANRIKLMMSIA 1415 NRIKLM+S A Sbjct: 301 LNRIKLMLSTA 311 >CAC2428 [I] COG1924 Activator of 2-hydroxyglutaryl-CoA dehydratase (HSP70-class ATPase domain) Length = 553 Score = 132 bits (333), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 25/321 (7%), Positives = 56/321 (17%), Gaps = 18/321 (5%) Query: 336 YLGIDAGSTTTKLVITGAADEILYTFYGSNKGNPLQSVSDALKEIYRILPEGCHIAGAYA 395 L + I + + S+ + +S + + + Sbjct: 3 NRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELT-STWDLML 61 Query: 396 TGYGEGLVKAALRTDGGEVETVAHYKAASKFMPEVDFILDIGGQDMKCIKIRGGAIDSLM 455 A + + + K + + + Sbjct: 62 QTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSR 121 Query: 456 LNEACSAGCGSFLESFASALGLGIGEFAAAALESAQPVNLGSRCTVFMNSKVKQVQKEGA 515 + + L L G A +G + S K + Sbjct: 122 NIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVT 181 Query: 516 TLADLSAGLAYSVIKNALQKVIKIRNADDLGRNIIVQGGTFYNEAVLRAFELLTGRTVVR 575 AD + + AL V+ I T + + E+ G Sbjct: 182 DNADDYRFAQWQLAVIALVVVLI---LLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANT 238 Query: 576 PDIAGVMGAYGCALLARE-------HAGFDGVSTLLGPEELEAFKYEVSPGRCRLCANNC 628 I G A + + Sbjct: 239 LTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREG--SDAIYAGTREIA-----AGNTDLSS 291 Query: 629 ALTISRFPDKSFHVTGNRCER 649 + + + Sbjct: 292 RTEQQASALEETAASMEQLTA 312 Score = 83.6 bits (205), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 16/265 (6%), Positives = 42/265 (15%), Gaps = 15/265 (5%) Query: 19 MLRIGLDVGSTTAKLVVMEHDV-IVYQDYVRH----FSDIKKAALSLLSDVRDRFPDREA 73 M+ V L V I + + Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGEL---TSTW 57 Query: 74 ALTVXXXXXXXXXXXXEVPFVQ-EVIACTKAISELIPQCDTAIELGGEDAKIIYLSGGI- 131 L + + + + + + + K + + Sbjct: 58 DLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMV 117 Query: 132 -EQRMNTACAGGTGAFIDQMASLLQTDPAGLNVLAEQHERIYPIASRCGVFAKSDVQPLL 190 R + ++ L G + +A S + Sbjct: 118 ATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYR 177 Query: 191 NEGARREDVAASIFQSIVNQTISGLACGRPIRGRVAFLGGPLTFLPALRERFAETLGLSD 250 + D + + + + + + G Sbjct: 178 DIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAK-IIAHIREIAGGNL- 235 Query: 251 SDVLFPEHSQYFVAIGSALSQTDPL 275 A S + Sbjct: 236 --ANTLTIDGRSEMGDLAQSVSHMQ 258 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 102,640,199 Number of extensions: 4404387 Number of successful extensions: 12005 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 11958 Number of HSP's successfully gapped: 18 Length of query: 1447 Length of database: 38,378,431 Length adjustment: 119 Effective length of query: 1328 Effective length of database: 24,653,685 Effective search space: 32740093680 Effective search space used: 32740093680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)