BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0015.p (336 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1308 [R] COG1073 Hydrolases of the alpha/beta superfamily 108 8e-24 lin2180 [R] COG1073 Hydrolases of the alpha/beta superfamily 75 1e-13 DR2478 [R] COG1073 Hydrolases of the alpha/beta superfamily 71 3e-12 BS_yfhR [IQR] COG1028 Dehydrogenases with different specificitie... 70 4e-12 yfhR [R] COG1073 Hydrolases of the alpha/beta superfamily 70 4e-12 >BH1308 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 321 Score = 108 bits (271), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 77/276 (27%), Positives = 133/276 (48%), Gaps = 9/276 (3%) Query: 59 YIAWVLSNPTVAPLYSNPSMAKGLDYEDITFPALDGSRMMQGWYIPSK------GSSKTI 112 Y+ W LS+P + +P+ GL +ED+ F + ++GW+IP++ G+ + + Sbjct: 42 YVGWNLSHPEREVIDESPT-DYGLLFEDVVFYSEKDEVELKGWWIPAQDNGEELGTDRAV 100 Query: 113 VFSHGYGANREETWVPMYDLAHYAHSLNFNVVMFDYGFASKTNKDIATGGKKESQQLLGA 172 VFSHGY +R + + A +++++FDY + ++ T G+ E+ LL A Sbjct: 101 VFSHGYRHSRLQGENDILPFAKRLAQEGYHLLLFDYRGSGESGGTYTTIGQYETDDLLSA 160 Query: 173 IRYAK-EHGAKEIVVWGFSMGAGTALQAGLVTKDVDAMILDSTFLLEPDTLYHNIKQNID 231 I + K E +EI V G+SMGA +A+ A ++DV +I DS F L N+ D Sbjct: 161 IAFVKAEKHVEEIAVIGWSMGAVSAILATQQSEDVQIVIADSPFANLRQYLSENLSHWSD 220 Query: 232 LPRQPSLEIMELLFPVLNGTGLNQIPYTKVKAEDYPFPTLFLHGTNDDKAPYPIAEELAA 291 LP P ++ PVL G ++Q+ + +HG D+ P+ +E + Sbjct: 221 LPDVPFTWVVLQTIPVLIGADIDQVSPVDAVSPIGETKLFLIHGRWDEAIPHRDSEAI-F 279 Query: 292 NQSNPYSDSWIVKGSHHELLFREHPREYLRRVSAFL 327 ++ ++ W+ + H E EY R+ AFL Sbjct: 280 EAADGQAELWLPENEGHVKTINEQSEEYEERILAFL 315 >lin2180 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 319 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 68/246 (27%), Positives = 107/246 (43%), Gaps = 11/246 (4%) Query: 88 TFPALDGSRMMQGWYIPSKGSSKTIVFSHGYGANREETWVPMYDLAH-YAHSLNFNVVMF 146 T + DG +++ + S+ TI+ +HGY + V M LA Y +NV+M Sbjct: 75 TETSADGLKLVANYLEAPSPSNTTIILAHGYRGKSGK--VEMAGLARMYNQKFGYNVLMP 132 Query: 147 D-YGFASKTNKDIATG--GKKESQQLLGAIRYAKEHGAKEIVVWGFSMGAGTAL--QAGL 201 D K+I G +K+ Q + K EIV+ G SMG+ T L A Sbjct: 133 DARAHGESEGKNIGFGWPERKDYVQWTNQV-LDKNGDDTEIVLHGVSMGSSTVLMTSAEK 191 Query: 202 VTKDVDAMILDSTFLLEPDTLYHNIKQNIDLPRQPSLEIMELLFPVLNGTGLNQIPYTKV 261 + K V ++I D + L + +K LP+ P + + L+ V G ++ Sbjct: 192 LPKQVKSIIADCGYTSMDAELAYQLKAMFHLPKFPIIPMASLINKVKEGFFFSEASAINA 251 Query: 262 KAEDYPFPTLFLHGTNDDKAPYPIAEELAANQSNPYSDSWIVKGSHHELLFREHPREYLR 321 AE P ++HG +D P + EEL + + Y + W+VKG+ H F P Y Sbjct: 252 VAET-DLPIFYIHGDSDAFVPTSMVEELY-DATKSYKEKWVVKGAEHGQAFTVEPETYEE 309 Query: 322 RVSAFL 327 +V FL Sbjct: 310 KVRQFL 315 >DR2478 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 407 Score = 70.9 bits (172), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 75/288 (26%), Positives = 119/288 (41%), Gaps = 29/288 (10%) Query: 57 HGYIAWVLSNPTVAPLYSNPSMAKGLDYEDITFPALDGSRMMQGWYIPSKGSSK--TIVF 114 G +AWV + LY+ G +YED+ P G M W++P + + K IV Sbjct: 128 QGALAWVSTY-----LYNGTPAQLGAEYEDVLVPTEVGE--MPAWHMPPRHAEKDALIVV 180 Query: 115 SHGYGANREETWVPMYDLAHYAHSLNFNVVMFDYGFASKTNKDIATGGKKESQQLLGAIR 174 HG+G R + + + F YG A K K T G E++ ++ A+ Sbjct: 181 VHGHGGQRAQALRMLPAMLRTGCGSLFVTFRNAYG-APKVGKGYLTLGDTEAEDVVAALA 239 Query: 175 YAKEHGAKEIVVWGFSMGAGTALQAGLVTK------DVDAMILDSTFLLEPDTLYHNIKQ 228 +A+E G K I+++GFSMG L L K + ++LDS L DT+ Q Sbjct: 240 WAREQGYKRIILFGFSMGGNIVLSV-LRPKFEPYPLPIVGVMLDSPALEWRDTIRWQ-AQ 297 Query: 229 NIDLPRQPSLEIMELLFPVLNGTG------LNQIPYTKVKAEDYPFPTLFLHGTNDDKAP 282 LP + + ++ ++QI A + P L HGT D P Sbjct: 298 RFGLPGFLARRVGRFTQRIVTQRSGQDFDVVDQI----AAAPHFKVPILMWHGTRDHTIP 353 Query: 283 YPIAEELAANQSNPYSDSWIVKGSHHELLFREHPREYLRRVSAFLGNV 330 AE L A + + + V+ + H + P+EY ++ F+ V Sbjct: 354 LAQAEALYAARPD-LVEFHRVEDAKHIRTWNISPKEYDGQLERFVARV 400 >BS_yfhR [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 293 Score = 70.5 bits (171), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 69/251 (27%), Positives = 104/251 (41%), Gaps = 32/251 (12%) Query: 85 EDITFPALDGSRMMQGWYIPSK-----GSSKTIVFSHGYGANREETWVPMYDLAHYAHSL 139 E + F A DG+R+ QGW+IPS + TI+ +HG N W L + Sbjct: 59 ESVEFTAKDGTRL-QGWFIPSSTGPADNAIATIIHAHGNAGNMSAHW----PLVSWLPER 113 Query: 140 NFNVVMFDY-GFASKTNKDIATGGKKESQQLLGAIRYAKEHGAKEIVVWGFSMGAGTALQ 198 NFNV MFDY GF G ++Q + +R+ + + +V++G S+G L Sbjct: 114 NFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGANILD 173 Query: 199 A-GLVTKD-VDAMILDSTFLLEPDTLYHNIKQNIDLPRQPSLEIMELLFPVLNGTGLNQI 256 G ++ + A+ILDSTF Y I + +P L +G N I Sbjct: 174 VIGRGDREGIRAVILDSTF-----ASYATIANQM-IPGSGYLLDESY-------SGENYI 220 Query: 257 PYTKVKAEDYPFPTLFLHGTNDDKAPYPIAEELAANQSNPYSDSWIVKGSHHELLFREHP 316 A P P L +HG D P+ +E+L + P I G H + H Sbjct: 221 ------ASVSPIPLLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHG 274 Query: 317 REYLRRVSAFL 327 Y ++ F+ Sbjct: 275 DVYREQMVDFI 285 >yfhR [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 293 Score = 70.5 bits (171), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 69/251 (27%), Positives = 104/251 (41%), Gaps = 32/251 (12%) Query: 85 EDITFPALDGSRMMQGWYIPSK-----GSSKTIVFSHGYGANREETWVPMYDLAHYAHSL 139 E + F A DG+R+ QGW+IPS + TI+ +HG N W L + Sbjct: 59 ESVEFTAKDGTRL-QGWFIPSSTGPADNAIATIIHAHGNAGNMSAHW----PLVSWLPER 113 Query: 140 NFNVVMFDY-GFASKTNKDIATGGKKESQQLLGAIRYAKEHGAKEIVVWGFSMGAGTALQ 198 NFNV MFDY GF G ++Q + +R+ + + +V++G S+G L Sbjct: 114 NFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGANILD 173 Query: 199 A-GLVTKD-VDAMILDSTFLLEPDTLYHNIKQNIDLPRQPSLEIMELLFPVLNGTGLNQI 256 G ++ + A+ILDSTF Y I + +P L +G N I Sbjct: 174 VIGRGDREGIRAVILDSTF-----ASYATIANQM-IPGSGYLLDESY-------SGENYI 220 Query: 257 PYTKVKAEDYPFPTLFLHGTNDDKAPYPIAEELAANQSNPYSDSWIVKGSHHELLFREHP 316 A P P L +HG D P+ +E+L + P I G H + H Sbjct: 221 ------ASVSPIPLLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHG 274 Query: 317 REYLRRVSAFL 327 Y ++ F+ Sbjct: 275 DVYREQMVDFI 285 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,392,639 Number of extensions: 1042178 Number of successful extensions: 1705 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 1693 Number of HSP's successfully gapped: 10 Length of query: 336 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 229 Effective length of database: 26,037,693 Effective search space: 5962631697 Effective search space used: 5962631697 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)