BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0012.p (572 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic ... 169 9e-42 BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic ... 88 4e-17 YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic... 69 2e-11 BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic... 66 2e-10 BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic... 57 1e-07 >BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 550 Score = 169 bits (429), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 134/501 (26%), Positives = 231/501 (46%), Gaps = 32/501 (6%) Query: 83 DNATDNVWTRYFKEKLNVQNKVAFEANDGTDYNQKVSMAIVGGT-IPDIMVVPDLSTLQQ 141 ++ DNV TR+ +E+L ++ + + +N ++ + + G +PD+ V D + Sbjct: 59 EDVHDNVVTRWAEEELGIKFVTEWTQPNDEQFNTQLRLMMSSGEKLPDVFQVNDTAIKAD 118 Query: 142 LYENDLIADLTDAYNQCASDQIKEIYDSYKGRVLDTARFDGKLMALPTTEISHGPG---V 198 L + L+ + +A AS ++K++++ + + + DG +P S G G + Sbjct: 119 LLSSGLVMPVDEAIETYASPRLKQLFEQFPQAFYPSTK-DGVRYGIP--RFSGGNGSDTL 175 Query: 199 LWLRKDWMDKLGLAEPKTMEDVENIITQFVEKDPGGNGAGNTVGLVVDNENTAGVSGGQY 258 LW+R+DW+DK L P T+E++E+I+ FV +DP G +T+G+ + G Sbjct: 176 LWIRQDWLDKFDLEPPTTLEELEHIMDVFVNEDPNETGKNDTIGITFAAGDYGFRGGNIA 235 Query: 259 ELNNIFTLYGAF-PKQWMDDGSGKAVYGSVQPEMKPALAKLADMYKKGLIDKQFAVRTAD 317 + + +F +G F P W G VYGS+QP MK LAK+ + Y+KG + FA+ +A Sbjct: 236 DSSWVFGAFGDFVPGAWSKGDDGSLVYGSIQPSMKDGLAKIHEWYEKGYLATDFAILSAG 295 Query: 318 DRKALLTSGKSGSFM-DNWWGSWTVADSIKLNPNAKWVSYVAPQSEDGSVTMFTGNPSSS 376 D + SG+SG W + + + ++ P+A + +G G Sbjct: 296 DAQESFISGRSGIMAAPPWAHGYPIGEMAQVVPDAIVKPFPHVSGPNGQA----GRRGEG 351 Query: 377 YLV----VRKGFEHPEAAIKISSLQFDYQRYQEKDEAVLKKFEDYASLNVGGSPIAINID 432 Y+V K FEH + + + Y + E D FE Y + G P+ Sbjct: 352 YVVGSFLFNKDFEHMDKFFEYWDAIYGYT-FGESDYFEYGLFEGYDYVMEDGEPV----- 405 Query: 433 YYDAFYRNVGIMEEALKTGDSSKLLTSN----DRNAYRSYKKYLDNVKNGEKLDSNGWAG 488 Y A + + EE + G L +N Y +++ + + E G AG Sbjct: 406 YDHAQFEEI-TGEEVVDPG--RYFLPTNVPTIPFEMYSLLEEFHETGREPETGYEAGLAG 462 Query: 489 YTSSITTANLVKNSK--IKEVNPVFFGTTPSMALKWPTLTKMELEMFLKIITGEQSPDAF 546 A + N + ++ +N TP+M + L +ME E+F+ II G + DAF Sbjct: 463 RDPDYIEAAAIVNRQNDLRIINEFNGPPTPTMVSRSEHLERMEDELFVDIIYGNKPLDAF 522 Query: 547 DSFVSSWKKTGGDTITNEVNE 567 D FV W+++GG+ IT EVNE Sbjct: 523 DQFVEEWRQSGGEAITEEVNE 543 >BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 512 Score = 88.2 bits (217), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 100/460 (21%), Positives = 184/460 (40%), Gaps = 57/460 (12%) Query: 117 KVSMAIVGGTIPDIMVVPDLSTLQQLYENDLIADLTDAYNQCASDQIKEIYDSYKG---- 172 +V+ A+ G +P ++ + D T + + +A+ +I D + Sbjct: 95 RVNAALASGNLPKVITLQDFRT----------SSVLNAFRSGQFWEIGPYLDEFPNLSEM 144 Query: 173 --RVLDTARFDGKLMALPTTEISHGPGVLWLRKDWMDKLGLAEPKTMEDVENIITQFVEK 230 +L+ DGK+ + GV+ +R+DW++ LGL P +E++ + F Sbjct: 145 NETILENVSVDGKIYGIYRERPLSRQGVV-IRQDWLENLGLEMPTNLEELYEVARAFTYD 203 Query: 231 DPGGNGAGNTVGLVVDNENTAGVSGGQYELNNIFTLYGAFPKQWMDDGSGKAVYGSVQPE 290 DP G+GA +TVG N+ G Y P +W D GK + + E Sbjct: 204 DPNGSGADDTVGFTDRNDLIFGAF-------KTLASYHGTPNEWGFDDEGKLMPEFMTDE 256 Query: 291 MKPALAKLADMYKKGLIDKQFAVRTADDRKALLTSGKSGSFMDNWWGSWTVADSIK-LNP 349 + + +Y +GLI+ +F V + + ++ L +G++G ++ N + + D K +NP Sbjct: 257 YVETMNYMKSLYDEGLINTEFPVTSKEQQQELFMTGRAGIYVGNMVDARNLRDKAKDINP 316 Query: 350 NAKWVSYVAPQSEDGSVTMFTGNPSSSYLVVRKGFEHPEAAIKISSLQFDYQRYQEKDEA 409 + DG ++ + + K EA +K FD R E+D Sbjct: 317 EMEVAITNRIAGPDGEDRVWAQGGHNGIFAIPKSSVETEAELKRILAFFD--RIAEEDVY 374 Query: 410 VLKKF--EDYASLNVGGSPIAINIDYYDAFYRNVGIMEEALKTGDSSKLLTSNDRNAYRS 467 L + ED + G +Y++ N+ ++ S L + + N ++ Sbjct: 375 NLMAYGIEDVHYETIDG-------EYFERIEENLDDWAAEVQPLVS---LVAREMNYLKA 424 Query: 468 YKKYLDNVKNGEKLDSNGWAGYTSSITTANLVKNSKIKEVNPVFFGTTPSMALKWPTLTK 527 Y L + E+L + N++I NP +P+ + L K Sbjct: 425 YGDPL--YEEYERLTED----------------NNEIIVTNPAEPLFSPTYTERGTELQK 466 Query: 528 MELEMFLKIITGEQSPDAFDSFVSSWKKTGGDTITNEVNE 567 + + K I GE D F++ V W GG++I E+NE Sbjct: 467 IIDDATYKYILGELDEDGFEAEVERWLTNGGESIIEELNE 506 >YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 437 Score = 69.3 bits (168), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 52/187 (27%), Positives = 84/187 (44%), Gaps = 22/187 (11%) Query: 77 GTPYEKDNATDNVWTRYFKEKLNVQNKVA--FEANDG---------TDYNQKVSMAIVGG 125 G P T VW R + N+ K A FE G TD+ Q+++ A G Sbjct: 24 GIPSLHAAETLQVWIRASNDSKNIYKKEADTFEKKTGIKIEYFNATTDFEQRLARAAAGN 83 Query: 126 TIPDIMVVPDLSTLQQLYENDLIADLTDAYNQCASDQIKEIYDSYKGRVLDTARFDGKLM 185 +PD ++ D + Q + ++ + + N + I + ++K T DGK Sbjct: 84 ALPD-LIFNDAVAIGQFVQLGIVEPI-EPKNIIGGENIFDT--AWK----STQYIDGKYY 135 Query: 186 ALPTTEISHGPGVLWLRKDWMDKLGLAEPKTMEDVENIITQFVEKDPGGNGAGNTVGLVV 245 +PT+ + L++RKDW +KLGL +PK +D+ + F DP GNG +T G ++ Sbjct: 136 GVPTSAQTFA---LFVRKDWREKLGLEQPKNWQDISTLAKAFTFNDPDGNGKNDTYGFIL 192 Query: 246 DNENTAG 252 T G Sbjct: 193 PGSTTRG 199 >BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 502 Score = 65.9 bits (159), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 58/227 (25%), Positives = 108/227 (47%), Gaps = 21/227 (9%) Query: 112 TDYNQKVSMAIVGGTIPDIMVVPD-----LSTLQQLYENDLIADLTDAYNQCASDQIKEI 166 + YN K ++ + G +P +V+ D + +++ +L L D N +D+ Sbjct: 76 SSYNDKFNIVMASGEMPHAIVIKDKSAGFIKSVKAGAFWELSPYLKDYKNLSQADE---- 131 Query: 167 YDSYKGRVLDTARFDGKLMALPTTEISHGPGVLWLRKDWMDKLGLAEPKTMEDVENIITQ 226 ++L + +G++ + T + +R DW+ +GL P+T++D ++ Sbjct: 132 ------KILKNSSVNGEVYGIYRTR-DLIRACMIIRTDWLKNVGLDMPETLDDFYEVLKA 184 Query: 227 FVEKDPGGNGAGNTVGLVVDNENTAGVSGGQYELNNIFTLYGAFPKQWMDDGSGKAVYGS 286 F EKDP GNG +T G+VV G +G +++ I+ +GA P ++ + +GK + Sbjct: 185 FKEKDPDGNGKDDTYGMVVPKWMGLG-NGSPWDVLQIW--FGA-PNRYGVE-NGKLIPDF 239 Query: 287 VQPEMKPALAKLADMYKKGLIDKQFAVRTADDRKALLTSGKSGSFMD 333 E AL +Y +GLI+K FAV + + GK+G +D Sbjct: 240 TTKEYMDALTFFKKLYDEGLINKDFAVMDSAKWNDPVVKGKAGVIVD 286 >BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 440 Score = 56.6 bits (135), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 58/241 (24%), Positives = 107/241 (44%), Gaps = 31/241 (12%) Query: 117 KVSMAIVGGTIPDIMVVPDL--STLQQLYENDLIADLTDAYNQCASDQIKEIYDSYKGRV 174 +++ A+ G +P I+ + D+ S++ + + + ++ D IK+ + K Sbjct: 75 RLNAALAAGNLPQIVTIQDIKNSSVMNAFRSGMFWEI--------GDYIKDYPNLNKMNK 126 Query: 175 L--DTARFDGKLMALPTTEISHGPGVLWLRKDWMDKLGLAEPKTMEDVENIITQFVEKDP 232 L DGKL + G++ +RKDW+D L L PKT++++ + F E DP Sbjct: 127 LINKNVTIDGKLYGIYRERPLSRQGIV-IRKDWLDNLNLKTPKTLDELYEVAKAFTEDDP 185 Query: 233 GGNGAGNTVGLVVDNENTAGV--SGGQYELNNIFTLYGAFPKQWMDDGSGKAVYGSVQPE 290 +G +T GL N+ G + G YE P W + G GK + E Sbjct: 186 DKDGKDDTFGLADRNDLIYGAFKTIGSYE---------GMPTDWKESG-GKFTPDFMTQE 235 Query: 291 MKPALAKLADMYKKGLIDKQFAVRTADDRKALLTSGKSGSFMDNWWGSWTVADSIKLNPN 350 K + + + G ++K F V + ++ L + GK+G ++ N + D++ L + Sbjct: 236 YKDTMNYMKKLRDNGYMNKDFPVTSKTQQQELFSQGKAGIYIGN------MVDAVNLRDH 289 Query: 351 A 351 A Sbjct: 290 A 290 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.129 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 44,745,289 Number of extensions: 1985271 Number of successful extensions: 3298 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 3290 Number of HSP's successfully gapped: 7 Length of query: 572 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 460 Effective length of database: 25,461,023 Effective search space: 11712070580 Effective search space used: 11712070580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 118 (50.1 bits)