BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0009.p (748 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TVN0429 [G] COG3408 Glycogen debranching enzyme 139 2e-32 MA2628 [G] COG3408 Glycogen debranching enzyme 137 8e-32 Ta0341 [G] COG3408 Glycogen debranching enzyme 128 4e-29 NMA0905 [MU] COG3468 Type V secretory pathway, adhesin AidA 126 1e-28 MA0905 [G] COG3408 Glycogen debranching enzyme 126 1e-28 >TVN0429 [G] COG3408 Glycogen debranching enzyme Length = 601 Score = 139 bits (350), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 113/360 (31%), Positives = 179/360 (49%), Gaps = 35/360 (9%) Query: 250 DGIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAKFQNTDAASKYFGRVPN 309 D I AG WF WGRD FI+LPG +L+ G ++ A N+L +++ Q+ D G + N Sbjct: 261 DNIIAGFHWFGP-WGRDAFISLPGLLLIPGMYDKAANVLRHYSE-QSVD------GLILN 312 Query: 310 ILALKNVDYHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQNSI----EGSIKHWVDD 365 NV Y + D + F+ +Q + +Y+ + A + E+Y VQN I +G+ ++ Sbjct: 313 SAEGGNVLY-SADSSLWFVYAIQKFYEYTKNKAFLREMYNKVQNIIYAYLKGNENFHIEG 371 Query: 366 KGYLMHDDNETWMDARDANLKSYSPRDTRANDIQALWYNQLQAGVYFAEYMGDSGSAKKW 425 K + TWMDA + K+ +PR +I ALWYN L+A F+ +G K+ Sbjct: 372 KFVRVTGSPMTWMDAV-IDGKAVTPRSGLPIEINALWYNTLKAYTDFSALLGKE-PKKEA 429 Query: 426 GNIASKLKRNFAADFRDQTHPYLADRLNPEDEPEFSLRPNQLFALDMIDDDSFKCEV-IK 484 I + F F TH + D P+D +S RPN LFA + K ++ I Sbjct: 430 IEILDGFEDEFKKAFVRGTH--ILDIAFPDD---YSFRPNFLFAY-FLPYPLLKDKIWID 483 Query: 485 TAWEELVYPWGVASLDQKHPFFHPFHLTSLYHKDEAYHNGTVWLWLNGIAMQRMIEAGQE 544 A ++LV P+G+ SLD +P F + + +D AYHNGTVW +L GI ++ I+ G + Sbjct: 484 YAIDKLVTPYGLRSLDPSNPSFIGKYAGDRFSRDNAYHNGTVWPYLAGIFIKAAIKVGYD 543 Query: 545 ETA-YKLFKNMNWQALNLGVVGGLSENMDAYPQEGQSWAKLTGAYLQAWSNAEQLRVWYQ 603 +FK + A + G++ + P+ G +QAWS+ E +R +Y+ Sbjct: 544 RIKLLNIFKPLFDSARIPEIYDGINPGV---PR---------GCIMQAWSHGEVIRSYYE 591 >MA2628 [G] COG3408 Glycogen debranching enzyme Length = 659 Score = 137 bits (344), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 110/394 (27%), Positives = 188/394 (47%), Gaps = 43/394 (10%) Query: 241 DQLVTRQQGDG---IYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAKFQNT 297 D V + G + AG W+++ WGRD I+LPG +L+ +FE A++ L +FA++ Sbjct: 285 DSFVVKNHATGENTVIAGYHWYSD-WGRDSMISLPGLLLIPYRFEEARSALNNFARYCRR 343 Query: 298 DAASKYFGRVPNILALKNVD--YHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQNSI 355 G +PN D Y+T D + FI L Y Y+ D +++++ V + I Sbjct: 344 -------GLIPNTFPAFGGDPIYNTVDASLWFIHALDRYFAYTQDFLFLSDVWDTVSSII 396 Query: 356 E----GSIKHWVDDKGYLMHDDNE-TWMDARDANLKSYSPRDTRANDIQALWYNQLQAGV 410 + G+ D YL+ + TWMDA+ + +PR +A ++ ALWYN L+ Sbjct: 397 DNYCAGTDFAIGMDSDYLIWQGPQLTWMDAKVGEW-AVTPRAGKACEVNALWYNALKTAS 455 Query: 411 YFAEYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDE----PEFSLRPNQ 466 Y +G+ S+ + +A+ + NF F + L D ++ ++ + ++RPNQ Sbjct: 456 YMGTLLGEDISSYE--TLAAGVASNFENTFWNPESNCLFDLIHQDEAGNQVKDPAIRPNQ 513 Query: 467 LFALD----MIDDDSFKCEVIKTAWEELVYPWGVASLDQKHPFFHPFHLTSLYHKDEAYH 522 +FA+ M+ + K ++ ++L+ P+G+ +L HP + + +D AYH Sbjct: 514 IFAVSLPYTMLSPEKEKA-IVDRVEKDLLTPFGLKTLSSDHPLYKGQYRGDALTRDTAYH 572 Query: 523 NGTVWLWLNGIAMQRMIEAGQE-----ETAYKLFKNMNWQALNLGVVGGLSENMDA-YPQ 576 NGTVW WL G ++ ++ E L + + L + +G +SE D YP Sbjct: 573 NGTVWPWLLGAYVKAYLKVNNSSKSSLEEMRVLLEGFD-AHLEVAGIGTISEVFDGDYPH 631 Query: 577 EGQSWAKLTGAYLQAWSNAEQLRVWYQYFLGFRP 610 G+ QAWS AE LR + + LG +P Sbjct: 632 APG------GSIAQAWSVAEILRAYVEDVLGIKP 659 >Ta0341 [G] COG3408 Glycogen debranching enzyme Length = 598 Score = 128 bits (321), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 103/367 (28%), Positives = 174/367 (47%), Gaps = 45/367 (12%) Query: 254 AGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFA-KFQNTDAASKYFGRVPNILA 312 AG WF WGRD FI++PG +LV+G++ A+ +L +A + Q+ G +PN Sbjct: 265 AGFHWFGP-WGRDTFISMPGVLLVSGRYNEARRVLEHYAERIQD--------GMIPNTQD 315 Query: 313 LKNVDYHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQNSIEGSIK----HWVDDKGY 368 ++ + + + D + FI +Q Y++Y+GD ++ +++ I IK +DD Sbjct: 316 VEALRF-SADTSLWFIYAVQKYLEYAGDDDFARQMLVPIKSIITAYIKGNSMFHLDDDLV 374 Query: 369 LMHDDNETWMDARDANLKSYSPRDTRANDIQALWYNQLQAGVYFAEYMGD--SGSAKKWG 426 + TWMDA + K +PR + +I ALWYN L +++ D + + Sbjct: 375 TLTGSPLTWMDAMVSG-KPVTPRIGKPIEINALWYNALS---FYSRTAHDITGEDTEMFS 430 Query: 427 NIASKLKRNFAADFRDQTHPYLADRLNPEDEPEFSLRPNQL----FALDMIDDDSFKCEV 482 +A ++K+ F A F + D +P+D RPN L +++D F Sbjct: 431 KLARRIKQKFRAKFIKGDA--VLDVSDPDD---MKFRPNFLIPYFLPYPLLNDRKF---- 481 Query: 483 IKTAWEELVYPWGVASLDQKHPFFHPFHLTSLYHKDEAYHNGTVWLWLNGIAMQRMIEAG 542 + AWE+LV P+G+ +L P + P + +D AYHNGTVW WL GI + ++ G Sbjct: 482 VDLAWEKLVTPYGLRTLSPDDPDYVPTYSGPQPERDMAYHNGTVWPWLAGIFITAAVKTG 541 Query: 543 QEETAYKLFKNMNWQALNLGVVGGLSENMDAYPQEGQSWAKLTGAYLQAWSNAEQLRVWY 602 ++ A L + + +S + + G K G +QAWS+ E R ++ Sbjct: 542 YDKHA--LLE-------KFAPLFSMSRIPEIFDGSGTGIPK--GCIMQAWSHGEIARAYF 590 Query: 603 QYFLGFR 609 + G R Sbjct: 591 EDLRGGR 597 >NMA0905 [MU] COG3468 Type V secretory pathway, adhesin AidA Length = 680 Score = 126 bits (316), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 111/396 (28%), Positives = 182/396 (45%), Gaps = 47/396 (11%) Query: 241 DQLVTRQQGDG---IYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAKFQNT 297 D + + G + AG WF + WGRD I+LPG LVT +F+ A++ L +F++ Sbjct: 306 DSFIVKNPSTGESSVIAGYHWFAD-WGRDTMISLPGLFLVTRRFDEARSTLKNFSRHCRR 364 Query: 298 DAASKYFGRVPNILALKNVD--YHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQNSI 355 G VPN + Y+T D + FI L Y Y+ D ++E++ V++ I Sbjct: 365 -------GLVPNAFPALGGEAVYNTVDASLWFIHSLGRYFAYTKDLLFLSEVWDTVESII 417 Query: 356 EGSIKHWVDDKGYLMHDD-------NETWMDARDANLKSYSPRDTRANDIQALWYNQLQA 408 + K D G M D TWMDA+ ++ +PR +A +I ALWYN L+ Sbjct: 418 DNYSKG--TDFGIGMDSDFLIRQGPQLTWMDAKVEDIP-VTPRAGKACEINALWYNALKT 474 Query: 409 GVYFAEYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDE----PEFSLRP 464 +G S+ + +A+ + +F F + L D + ++ + S+RP Sbjct: 475 ASRIGGLLGKETSS--YETLAAGVASSFEEVFWNPETNCLYDLVTKDEAGNQIKDSSIRP 532 Query: 465 NQLFAL----DMIDDDSFKCEVIKTAWEELVYPWGVASLDQKHPFFHPFHLTSLYHKDEA 520 NQ+FA+ ++ + K ++ ++L+ P+G+ +L +P + + +D A Sbjct: 533 NQIFAVALPYTLLSPEKEKA-IVDRVEKDLLTPFGLRTLSTDNPSYKGRYHGGPKERDLA 591 Query: 521 YHNGTVWLWLNG--IAMQRMIEAGQE---ETAYKLFKNMNWQALNLGVVGGLSENMDA-Y 574 YHNGTVW WL G + R + + E L K + G +G +SE D + Sbjct: 592 YHNGTVWPWLLGAYVKAYRKVHNYSKKSLEDMRSLLKGFDMHLETAG-IGTISEVFDGDF 650 Query: 575 PQEGQSWAKLTGAYLQAWSNAEQLRVWYQYFLGFRP 610 P + G QAWS AE LR + + LG +P Sbjct: 651 PYSPE------GCIAQAWSVAEILRAYVEDVLGIKP 680 >MA0905 [G] COG3408 Glycogen debranching enzyme Length = 680 Score = 126 bits (316), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 111/396 (28%), Positives = 182/396 (45%), Gaps = 47/396 (11%) Query: 241 DQLVTRQQGDG---IYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAKFQNT 297 D + + G + AG WF + WGRD I+LPG LVT +F+ A++ L +F++ Sbjct: 306 DSFIVKNPSTGESSVIAGYHWFAD-WGRDTMISLPGLFLVTRRFDEARSTLKNFSRHCRR 364 Query: 298 DAASKYFGRVPNILALKNVD--YHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQNSI 355 G VPN + Y+T D + FI L Y Y+ D ++E++ V++ I Sbjct: 365 -------GLVPNAFPALGGEAVYNTVDASLWFIHSLGRYFAYTKDLLFLSEVWDTVESII 417 Query: 356 EGSIKHWVDDKGYLMHDD-------NETWMDARDANLKSYSPRDTRANDIQALWYNQLQA 408 + K D G M D TWMDA+ ++ +PR +A +I ALWYN L+ Sbjct: 418 DNYSKG--TDFGIGMDSDFLIRQGPQLTWMDAKVEDIP-VTPRAGKACEINALWYNALKT 474 Query: 409 GVYFAEYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDE----PEFSLRP 464 +G S+ + +A+ + +F F + L D + ++ + S+RP Sbjct: 475 ASRIGGLLGKETSS--YETLAAGVASSFEEVFWNPETNCLYDLVTKDEAGNQIKDSSIRP 532 Query: 465 NQLFAL----DMIDDDSFKCEVIKTAWEELVYPWGVASLDQKHPFFHPFHLTSLYHKDEA 520 NQ+FA+ ++ + K ++ ++L+ P+G+ +L +P + + +D A Sbjct: 533 NQIFAVALPYTLLSPEKEKA-IVDRVEKDLLTPFGLRTLSTDNPSYKGRYHGGPKERDLA 591 Query: 521 YHNGTVWLWLNG--IAMQRMIEAGQE---ETAYKLFKNMNWQALNLGVVGGLSENMDA-Y 574 YHNGTVW WL G + R + + E L K + G +G +SE D + Sbjct: 592 YHNGTVWPWLLGAYVKAYRKVHNYSKKSLEDMRSLLKGFDMHLETAG-IGTISEVFDGDF 650 Query: 575 PQEGQSWAKLTGAYLQAWSNAEQLRVWYQYFLGFRP 610 P + G QAWS AE LR + + LG +P Sbjct: 651 PYSPE------GCIAQAWSVAEILRAYVEDVLGIKP 680 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 54,399,958 Number of extensions: 2207036 Number of successful extensions: 4331 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 4308 Number of HSP's successfully gapped: 9 Length of query: 748 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 634 Effective length of database: 25,230,355 Effective search space: 15996045070 Effective search space used: 15996045070 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)