BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0004.p (205 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr2975 [U] COG0681 Signal peptidase I 126 2e-29 SA0826 [U] COG0681 Signal peptidase I 114 7e-26 BH1030 [U] COG0681 Signal peptidase I 108 6e-24 lin1308 [U] COG0681 Signal peptidase I 104 9e-23 BS_sipT [U] COG0681 Signal peptidase I 100 2e-21 >alr2975 [U] COG0681 Signal peptidase I Length = 359 Score = 126 bits (317), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 6/182 (3%), Positives = 28/182 (15%), Gaps = 8/182 (4%) Query: 24 RTGSLSGTGWVRDLRDWLVTAAIVFALMSLLNIFVFNVSTVIGQSMQPTLFEGEKLIISK 83 +L R + + + + + + + + E+ + + Sbjct: 9 NRRALRHNLQ-RLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLR 67 Query: 84 ISLTFASPRRSDVVVLHDPST----GPDRKEYLVKRVIGIPGDRIEVRNHKLYVNGKLIG 139 + + + + +++ Sbjct: 68 AGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGM 127 Query: 140 ESYVDTEIQDPDFAELTVKQGTYFVMGDNRHAGASKDSRYFGAVPQNRIVGKAAYIWWPL 199 Q + Q N + + Sbjct: 128 HRLGVRPEQAE-AFYHRLTQCKNVRQPVNIV--SHFARADEPKCGATEKQLAIFNTFCEG 184 Query: 200 SK 201 Sbjct: 185 KP 186 >SA0826 [U] COG0681 Signal peptidase I Length = 191 Score = 114 bits (286), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 60/184 (32%), Positives = 104/184 (56%), Gaps = 24/184 (13%) Query: 35 RDLRDWLVTAAIVFALMSLLNIFVFNVSTVIGQSMQPTLFEGEKLIISKISLTFASPRRS 94 ++L +W+++ A+ F ++ ++ F+ T+ G+SM PTL +GE++ ++ I + Sbjct: 3 KELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDGERVAVNIIGYKTGGLEKG 62 Query: 95 DVVVLHDPSTGPDRKEYLVKRVIGIPGDRIEVRNHKLYVNGKLIGESYVDTEIQDPDFAE 154 +VVV H ++ + VKRVIG+PGD++E +N LYVNGK E Y++ ++ Sbjct: 63 NVVVFH-----ANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQDEPYLNYNLKHKQGDY 117 Query: 155 LT-----------------VKQGTYFVMGDNRHAGASKDSRYFGAVPQNRIVGKAAYIWW 197 +T + +G Y V+GDNR SKDSR FG + +++IVGK ++ +W Sbjct: 118 ITGTFQVKDLPNANPKSNVIPKGKYLVLGDNRE--VSKDSRAFGLIDEDQIVGKVSFRFW 175 Query: 198 PLSK 201 P S+ Sbjct: 176 PFSE 179 >BH1030 [U] COG0681 Signal peptidase I Length = 182 Score = 108 bits (270), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 67/177 (37%), Positives = 96/177 (54%), Gaps = 14/177 (7%) Query: 36 DLRDWLVTAAIVFALMSLLNIFVFNVSTVIGQSMQPTLFEGEKLIISKISLTFASPRRSD 95 D+ +W+ I L L+ FVF V G+SM+PT +EGE I++K+S F+ P+R D Sbjct: 12 DINEWIKAILIALLLAVLIRSFVFVSYEVRGESMEPTAYEGEMFIVNKLSYEFSEPKRFD 71 Query: 96 VVVLHDPSTGPDRKEYLVKRVIGIPGDRIEVRNHKLYVNGKLIGESYVDTEIQ------D 149 ++V H T + +KR+IG+PGD I + + LY+N + E Y+D + Sbjct: 72 LIVFHATET-----DDYIKRIIGLPGDTIRMEDDILYINDEPYEEPYLDEWKEGRPGKYT 126 Query: 150 PDF-AELTVKQGTYFVMGDNRHAGASKDSRYFGAVPQNRIVGKAAYIWWPLSKANAL 205 DF E + G FV+GDNR S DSR FG VP IVGK +WP++K L Sbjct: 127 QDFVVEEPIPDGYVFVLGDNRP--RSSDSRAFGPVPLEEIVGKVGVRFWPVTKVGVL 181 >lin1308 [U] COG0681 Signal peptidase I Length = 188 Score = 104 bits (260), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 64/157 (40%), Positives = 89/157 (56%), Gaps = 31/157 (19%) Query: 57 FVFNVSTVIGQSMQPTLFEGEKLIISKISLTFASPRRSDVVVLHDPSTGPDRKEYLVKRV 116 F+ + TV G+SM PTL +GE L I+KIS P+R D++V P+ + EY +KRV Sbjct: 31 FLISPVTVNGKSMDPTLHDGEHLFINKIS----DPKRFDIIVF--PAPDEENAEY-IKRV 83 Query: 117 IGIPGDRIEVRNHKLYVNGKLIGESYVDTEIQ-------------DPDF---------AE 154 IG+PGD++E + +LY+NGK E Y+D+E DP+F Sbjct: 84 IGLPGDKVEYKEDQLYINGKKYDEPYLDSEKSALTGGYLTTDDKGDPNFTMADIPYSDGS 143 Query: 155 LTVKQGTYFVMGDNRHAGASKDSRYFGAVPQNRIVGK 191 LTV G FV+GDNR SKDSRY G + Q+ ++GK Sbjct: 144 LTVPDGKLFVLGDNRQ--VSKDSRYIGFISQDTVLGK 178 >BS_sipT [U] COG0681 Signal peptidase I Length = 193 Score = 100 bits (249), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 58/173 (33%), Positives = 95/173 (54%), Gaps = 16/173 (9%) Query: 39 DWLVTAAIVFALMSLLNIFVFNVSTVIGQSMQPTLFEGEKLIISKISLTFASPRRSDVVV 98 +W I L L+ F+F V G SM PTL +GE+L ++K +R D+V+ Sbjct: 22 EWGKAIVIAVLLALLIRHFLFEPYLVEGSSMYPTLHDGERLFVNKTVNYIGELKRGDIVI 81 Query: 99 LHDPSTGPDRKEYLVKRVIGIPGDRIEVRNHKLYVNGKLIGESYVDTEIQDP-------- 150 ++ G K + VKR+IG PG+ +++++ LY+NGK + E Y+ ++ Sbjct: 82 IN----GETSKIHYVKRLIGKPGETVQMKDDTLYINGKKVAEPYLSKNKKEAEKLGVSLT 137 Query: 151 -DFAELTVKQGTYFVMGDNRHAGASKDSRY-FGAVPQNRIVGKAAYIWWPLSK 201 DF + V +G YFVMGDNR S DSR G + ++RIVG + ++++P ++ Sbjct: 138 GDFGPVKVPKGKYFVMGDNRL--NSMDSRNGLGLIAEDRIVGTSKFVFFPFNE 188 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,690,331 Number of extensions: 622613 Number of successful extensions: 1363 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 1313 Number of HSP's successfully gapped: 30 Length of query: 205 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 104 Effective length of database: 26,729,697 Effective search space: 2779888488 Effective search space used: 2779888488 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 113 (48.1 bits)