BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0442.0024.p (276 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3665 [R] COG1073 Hydrolases of the alpha/beta superfamily 130 2e-30 DR1053 [R] COG1073 Hydrolases of the alpha/beta superfamily 79 6e-15 CPn0161 [R] COG1073 Hydrolases of the alpha/beta superfamily 75 1e-13 mlr6657 [R] COG1073 Hydrolases of the alpha/beta superfamily 74 3e-13 CPn0152 [R] COG1073 Hydrolases of the alpha/beta superfamily 62 7e-10 >CAC3665 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 265 Score = 130 bits (328), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 78/265 (29%), Positives = 131/265 (49%), Gaps = 15/265 (5%) Query: 1 MERNIIIRHNGEELTASIHYPAREKGTSGRCKDRVPLAVICHGFVGNRIGVDRIFVKAAR 60 M++++ I+ L +H P ++++P+ VI HGF GN++G IFVK AR Sbjct: 7 MQKSVEIKSKSLTLRGVLHMPLE-------AREKLPIVVIYHGFCGNKMGPHFIFVKLAR 59 Query: 61 ELAADGYMVIRFDYAGCGESSGNYGSEDMESMIAQTRAVLDYGISSADVDPQRITLIGHS 120 EL G IRFD+AG GES G + + + +LDY + VD RI+++G S Sbjct: 60 ELEKLGIATIRFDFAGTGESDGEFVDMTFSNEVYDANVILDYVKTLEFVDKDRISILGFS 119 Query: 121 LGGAVA-LLTGVRDRRVKNLVLWAAVGYPFNDIVK--IVGREAYDRAVKSGSADFAGYTF 177 +GGA+A ++ G R + L LWA G I+ +G + YD ++ G D G Sbjct: 120 MGGAIASVIAGDRKDEINTLCLWAPAGNMEQIILSDTYIG-DKYDEIMEKGIYDVEGLLL 178 Query: 178 TPVYFNSLASFQPFQEAGKFSGDVLVIHGTSDDVIPVDYAFLYQKLFWTRPEGRCDKEVI 237 + + F A ++ L+IHGTSD+++P+ + Y +++ E++ Sbjct: 179 GKKFLEDIKKVNIFDRASAYNKQSLIIHGTSDEIVPLSTSERYLEMYGE----NTSLELV 234 Query: 238 FQADHTFSSGPAQAQVMKRTKDWMN 262 A+H F + +V+ TK + + Sbjct: 235 EGANHIFEKNSWENRVIDLTKKYFS 259 >DR1053 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 236 Score = 79.3 bits (194), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 54/213 (25%), Positives = 89/213 (41%), Gaps = 7/213 (3%) Query: 10 NGEELTASIHYPAREKGTSGRCKDRVPLAVICHGFVGNRIGVDRIFVKAARELAADGYMV 69 G+ + +H P P VI HGF G+++ R+ V AR L A G Sbjct: 10 EGQRMYGMLHTPDGSATGQQAPPQGWPSVVIVHGFTGDKVSSHRLLVLLARRLTAAGIAA 69 Query: 70 IRFDYAGCGESSGNYGSEDMESMIAQTRAVLDYGISSADVDPQRITLIGHSLGGAVALLT 129 +RFD G GES G++ + + A DY +DP+R+ L+G+S+GG V+ L Sbjct: 70 LRFDCRGSGESQGDFSEMTVGREVQDVEAAFDYVRHQPGLDPERVMLLGYSMGGLVSALA 129 Query: 130 GVRDRRVKNLVLWAAVGYPFNDIVKIVGREAYDRAVKSGSADFAGYTFTPVYFNSLASFQ 189 R LW+ + ++ R + +D+ G+ + + + Sbjct: 130 -AEKVRPHRFALWSPA------LPELWLRHLRGGLLPPVISDYGGWPLGRAFLQEVVQTR 182 Query: 190 PFQEAGKFSGDVLVIHGTSDDVIPVDYAFLYQK 222 P + A ++ G V HG D PV++ K Sbjct: 183 PLEAAARWGGVAHVFHGDRDQTCPVEWGVRTPK 215 >CPn0161 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 275 Score = 74.7 bits (182), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 54/242 (22%), Positives = 104/242 (42%), Gaps = 25/242 (10%) Query: 36 PLAVICHGFVGNRIGVDRIFVKAARELAADGYMVIRFDYAGCGESSGNYGSEDMESMIAQ 95 P+ ++ HG ++ G R V+ A+EL G +R D G G+ G +E+ Sbjct: 41 PIVILLHGLASDKTGSKRSHVRLAQELTRLGIAALRVDLLGHGDCEGELMDFSLENYKQN 100 Query: 96 TRAVLDYGISSADVDPQRITLIGHSLGGAVALLTGVRDRRVKNLVLWAAVGYPFNDIVKI 155 R +++Y S +D +R+ + G SLGG +AL T ++K L +WA ++ Sbjct: 101 IREIIEYTHSLLHIDQERLAIFGSSLGGTLALQTLPFFNKIKALAVWAPTISG-----EL 155 Query: 156 VGREAYDRA------VKSGSADFAGYTFTPVYFNSLASFQPFQE---AGKFSGDVLVIHG 206 + EA A + G+ +AG T P ++ +E + + +L + G Sbjct: 156 MAAEAQKNAPEVITMSQKGAITYAGMTLNPDFYTQFLKIDIVKELMPSARNLPPILYMQG 215 Query: 207 TSDDVIPVDYAFLYQKLFWTRPEGRCDKEVIF----QADHTFSSGPAQAQVMKRTKDWMN 262 D ++ +++ L+ + F + DK + DH F A++ + W+ Sbjct: 216 EQDLLVSINHRTLFTEAFANQ-----DKPITILTYPDVDHAFPF--AESSALSDLTQWLK 268 Query: 263 QQ 264 ++ Sbjct: 269 RE 270 >mlr6657 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 295 Score = 73.6 bits (179), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 59/227 (25%), Positives = 96/227 (42%), Gaps = 26/227 (11%) Query: 2 ERNIIIRHNGEELTASIHYPAREKGTSGRCKDRVPLAVI-CHGFVGNR-------IGVDR 53 E+ + I +G L ++ P C P V+ HGF G + G+ Sbjct: 23 EKVVRIDSDGRGLAGILNRP---------CGIPAPAVVLMLHGFTGQKNEFQLAKTGIG- 72 Query: 54 IFVKAARELAADGYMVIRFDYAGCGESSGNYGSEDMESMIAQTRAVLDYGISSADVDPQR 113 +F AA +LA G +R D+ G G+S+GN+ I DY + DVD R Sbjct: 73 LFAYAAAKLAEQGIATLRIDFNGSGDSAGNWIDTTFSGQINDAMLAYDYLQTLRDVDGSR 132 Query: 114 ITLIGHSLGGAVALLTGVRDRRVKNLVLWAAVGYPFNDIVKIVGREAYDRAVKSGSAD-- 171 + ++G+S GG VA + LVLWA V P + +VG E RA+ +++ Sbjct: 133 VGILGYSQGGLVASHVAALRPQASALVLWAPVTNPMSTFSSLVGAETVARAIAGEASEEI 192 Query: 172 -----FAG-YTFTPVYFNSLASFQPFQEAGKFSGDVLVIHGTSDDVI 212 + G T ++ L F P ++ G + V+ G + ++ Sbjct: 193 RTKLSWGGETTLKAAFYKELPLFAPAVAISRYHGPLRVVVGRREMIV 239 >CPn0152 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 316 Score = 62.4 bits (150), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 48/173 (27%), Positives = 76/173 (43%), Gaps = 15/173 (8%) Query: 5 IIIRHNGEELTASIHYPAREKGTSGRCKDRVPLAVICHGFVGNRIG-VDRIFVKAARELA 63 + I+ + L +H P G P V+ HGF G + G + + K R+ A Sbjct: 52 VTIKCDDHNLIGVLHLPNTPTPEGG-----FPTVVLFHGFRGTKFGGLTGAYRKLGRKFA 106 Query: 64 ADGYMVIRFDYAGCGESSGNYGSEDMESMIAQTRAVLDYGISSADVDPQRITLIGHSLGG 123 A G +R D AGCG+S G +E+ + + +L+ D++ R+ + G SLG Sbjct: 107 AVGIATLRVDMAGCGDSEGVAEEVPIETYLRDAQTILETVQEHPDLNAYRLGISGFSLGC 166 Query: 124 AVAL----LTGVRDRRVKNLVLWAAVGYPFNDIVKIVGREAYDRAVKSGSADF 172 +A + RD +K L +WA P D I+ +E Y+ K G D Sbjct: 167 HIAFELAKIYNPRDLNIKALSVWA----PIAD-GGILLKELYENFSKHGEGDI 214 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.137 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,625,883 Number of extensions: 873530 Number of successful extensions: 1809 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 1828 Number of HSP's successfully gapped: 14 Length of query: 276 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 171 Effective length of database: 26,268,361 Effective search space: 4491889731 Effective search space used: 4491889731 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)