BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0442.0017.p (181 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3407 [R] COG1073 Hydrolases of the alpha/beta superfamily 97 1e-20 >CAC3407 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 431 Score = 97.1 bits (240), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 57/174 (32%), Positives = 96/174 (55%), Gaps = 8/174 (4%) Query: 5 WCSGDEFSSYAVFVADETGKVDFDLAKPVEGSYETANSMGLIYSLKQSTSTGKNIAENIS 64 W F S+AVF AD+ G VD P++GSY +SMGL +S+ + + +++S Sbjct: 56 WVKKTNFESFAVFKADDKGTVDLSKDAPIKGSYTRVDSMGLFWSMVSTQQQDYTLKKSLS 115 Query: 65 IEKPII--MHLLFESSIERKEV---QLKRLFKAEDILVKQINES-FTGQLFYQKNTN-NK 117 P + M ++ + + KE+ + K+LF + D+ + + E+ G+ F KN+ K Sbjct: 116 -SCPFLTSMEVILKVEVCGKELVSSKCKKLFVSSDVKCQYVTENNLVGKYFTNKNSKIKK 174 Query: 118 TVLMVGGSDGQMQSLALMAGPLASRGFNVLITSYFASKGLPEKLEEVPLEYFEK 171 ++++ GS+G + + + +A LAS G+ L +YF LP L E+PLEYFEK Sbjct: 175 GIIVLSGSEGGIHNASQIAALLASHGYPSLALAYFGMDTLPATLNEIPLEYFEK 228 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,017,479 Number of extensions: 445376 Number of successful extensions: 919 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 918 Number of HSP's successfully gapped: 1 Length of query: 181 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 81 Effective length of database: 26,845,031 Effective search space: 2174447511 Effective search space used: 2174447511 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 112 (47.8 bits)