BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0442.0012.p (515 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1395 [F] COG0138 AICAR transformylase/IMP cyclohydrolase PurH... 517 e-146 YLR028c [F] COG0138 AICAR transformylase/IMP cyclohydrolase PurH... 270 6e-72 YMR120c [F] COG0138 AICAR transformylase/IMP cyclohydrolase PurH... 269 6e-72 CAC2445 [F] COG0138 AICAR transformylase/IMP cyclohydrolase PurH... 80 7e-15 >CAC1395 [F] COG0138 AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful) Length = 499 Score = 517 bits (1332), Expect = e-146, Method: Compositional matrix adjust. Identities = 263/514 (51%), Positives = 357/514 (69%), Gaps = 17/514 (3%) Query: 3 IKRALVSVSDKQGIVDFCRGLSALGVEIISTGGTSTLLAKEGVPVIGISDVTGFPEIMDG 62 IKRAL+SV +K GI+ + L+ GVEI+STGGT L + G+PVI +S+VTGF EI+DG Sbjct: 2 IKRALISVFNKNGILKLAKFLNEKGVEILSTGGTYKHLKENGIPVIEVSEVTGFDEILDG 61 Query: 63 RVKTLHPAVHSGLLAVRDNEEHTRQMQELGLDYIDLVVVNLYPFAETIAKADVSYEEAIE 122 RVKTLHP +H G+LA+RDN+EH ++ G+ ID+VVVNLYPF + + K ++S+EE +E Sbjct: 62 RVKTLHPKIHGGILAIRDNKEHMDTIKNKGITPIDMVVVNLYPFFDKV-KENISFEEKVE 120 Query: 123 NIDIGGPTMLRSAAKNHAFVSVVVDAADYATVLEEVRAGGDTTLETRKRLAAKVFRHTAA 182 IDIGGPTM+R+AAKN V V+ D DY V++++ G+ T+KRLA KVF +A Sbjct: 121 FIDIGGPTMIRAAAKNFQDVVVLTDVNDYDDVIDQIEKTGEVAYNTKKRLAGKVFNLMSA 180 Query: 183 YDALISDYLANVTGEPLPERYTVTYEKIQDLRYGENPHQKAAFYRKPLAAQDTLTSAEQL 242 YD IS +L + PE V Y+K DLRYGENPHQ AAFY + EQL Sbjct: 181 YDGAISRFLLE---DEYPEYLAVPYKKKMDLRYGENPHQTAAFYEAAFG-DGAMKGFEQL 236 Query: 243 HGKELSYNNINDANAALQIVKEFEEPAVVAVKHMNPCGVGVGASVYEAYQKAYNADPTSI 302 +GKELSYNNI D + A ++V EF+E A+KH +PCGV +G + +AY+KA+ D TSI Sbjct: 237 NGKELSYNNIKDMDIAWKVVNEFDETVCCALKHNSPCGVAIGENPLDAYKKAFECDDTSI 296 Query: 303 FGGIVAANRIIDADTANMLKDIFLEIVLAPGFTEEALDILTKKKNIRLLKLGNLSTAAAR 362 FGGIVA N++ID A + IFLEIV+AP FT +AL++L KKN+R++K + Sbjct: 297 FGGIVALNKVIDKPAAEEMVKIFLEIVIAPDFTADALEVLKSKKNLRVIKANEKPS---- 352 Query: 363 KSSFVVTSIEGGMVVQESDVHSVNPEELQVVTNRKPTEEELKQLLFGWKVVKHVKSNAIV 422 +F + ++G M+VQ +D E+++VVT++KPT+EE++ ++FG KVVK+VKSNAIV Sbjct: 353 -DNFELAKVDGAMLVQSADNKLT--EKMEVVTDKKPTDEEMRDMIFGMKVVKYVKSNAIV 409 Query: 423 LAADDMTVGVGAGQMNRVGAAKIAIEQAGEKAKG-SVLASDAFFPMGDTLEMAAKAGITA 481 + + VG+G GQ+NR+ AK A+ E+ KG +VLASDAFFP GD +E A K GI A Sbjct: 410 VVKNGAAVGIGGGQVNRIWPAKDAM----ERGKGAAVLASDAFFPFGDIVEEAHKNGIKA 465 Query: 482 VIQPGGSIKDEESIKVANEYGIAMVFTGVRHFKH 515 +IQPGGSI+D+ESI N+YGI+MV TG+RHFKH Sbjct: 466 IIQPGGSIRDQESIDGCNKYGISMVMTGIRHFKH 499 >YLR028c [F] COG0138 AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful) Length = 591 Score = 270 bits (689), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 173/477 (36%), Positives = 261/477 (54%), Gaps = 59/477 (12%) Query: 4 KRALVSVSDKQGIVDFCRGLSALGVEIISTGGTSTLLAKEGVPVIGISDVTGFPEIMDGR 63 K A++SV DK G++D +GL V I+++GGT+ ++ + G PV +S +T PE++ GR Sbjct: 6 KTAILSVYDKTGLLDLAKGLVENNVRILASGGTANMVREAGFPVDDVSSITHAPEMLGGR 65 Query: 64 VKTLHPAVHSGLLAVRDNEEHTRQMQELGLDYIDLVVVNLYPFAETIAKADVSYEEAIEN 123 VKTLHPAVH+G+LA R+ E + ++E +D +D VV NLYPF ET+AK V+ +EA+E Sbjct: 66 VKTLHPAVHAGILA-RNLEGDEKDLKEQHIDKVDFVVCNLYPFKETVAKIGVTVQEAVEE 124 Query: 124 IDIGGPTMLRSAAKNHAFVSVVVDAADYATVLEEVRAGGDTTLETRKRLAAKVFRHTAAY 183 IDIGG T+LR+AAKNH+ V+++ D DY+ L+++ G+ + + R R A K F HTA Y Sbjct: 125 IDIGGVTLLRAAAKNHSRVTILSDPNDYSIFLQDLSKDGEISQDLRNRFALKAFEHTADY 184 Query: 184 DALISDYLANVTGE---PLPERYTVTYEKIQDLRYGENPHQKAAFYRKPLAAQDTLTSAE 240 DA ISD+ E LP LRYG NPHQ+ AQ +T E Sbjct: 185 DAAISDFFRKQYSEGKAQLP------------LRYGCNPHQR--------PAQAYITQQE 224 Query: 241 QLHGKELS----YNNINDANAALQIVKEFEE----PAVVAVKHMNPCGVGVGASVYE--- 289 +L K L Y N+ DA + +VKE PA + KH++P G VG + + Sbjct: 225 ELPFKVLCGTPGYINLLDALNSWPLVKELSASLNLPAAASFKHVSPAGAAVGLPLSDVER 284 Query: 290 ----------------AYQKAYNADPTSIFGGIVAANRIIDADTANMLKDIFLEIVLAPG 333 AY +A AD S FG +A + I+D TA ++ + V+APG Sbjct: 285 QVYFVNDMEDLSPLACAYARARGADRMSSFGDFIALSNIVDVATAKIISKEVSDGVIAPG 344 Query: 334 FTEEALDILTKKKNIR--LLKLGNLSTAAARKSSFVVTSIEGGMVVQESDVHSVNPEELQ 391 + EAL+IL+KKKN + +L++ +S V G + Q+ + +N + Sbjct: 345 YEPEALNILSKKKNGKYCILQIDPNYVPGQMESREVF----GVTLQQKRNDAIINQSTFK 400 Query: 392 --VVTNRKPTEEELKQLLFGWKVVKHVKSNAIVLAADDMTVGVGAGQMNRVGAAKIA 446 V N+ TE+ + L V+K+ +SN++ A + M VG+GAGQ +R+ ++A Sbjct: 401 EIVSKNKALTEQAVIDLTVATLVLKYTQSNSVCYAKNGMVVGLGAGQQSRIHCTRLA 457 >YMR120c [F] COG0138 AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful) Length = 592 Score = 269 bits (688), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 173/476 (36%), Positives = 262/476 (55%), Gaps = 49/476 (10%) Query: 4 KRALVSVSDKQGIVDFCRGLSALGVEIISTGGTSTLLAKEGVPVIGISDVTGFPEIMDGR 63 K A++SV DK G++D RGL V I+++GGT+ ++ G P+ +S +T PE++ GR Sbjct: 6 KTAILSVYDKTGLLDLARGLIEKNVRILASGGTARMIRDAGFPIEDVSAITHAPEMLGGR 65 Query: 64 VKTLHPAVHSGLLAVRDNEEHTRQMQELGLDYIDLVVVNLYPFAETIAKADVSYEEAIEN 123 VKTLHPAVH G+LA RD + + ++E ++ +D VV NLYPF ET+AK V+ EA+E Sbjct: 66 VKTLHPAVHGGILA-RDIDSDEKDLKEQHIEKVDYVVCNLYPFKETVAKVGVTIPEAVEE 124 Query: 124 IDIGGPTMLRSAAKNHAFVSVVVDAADYATVLEEVRAGGDTTLETRKRLAAKVFRHTAAY 183 IDIGG T+LR+AAKNHA V+++ D DY+ L E+ + G+ + + R RLA K F HTA Y Sbjct: 125 IDIGGVTLLRAAAKNHARVTILSDPKDYSEFLSELSSNGEISQDLRNRLALKAFEHTADY 184 Query: 184 DALISDYLANVTGEPLPERYTVTYEKIQDLRYGENPHQKAAFYRKPLAAQDTLTSAEQLH 243 DA ISD+ ++Y+ +I LRYG NPHQK A + ++ QD+L + L Sbjct: 185 DAAISDFFR--------KQYSEGQAQIT-LRYGANPHQKPA--QAYVSQQDSL-PFKVLC 232 Query: 244 GKELSYNNINDANAALQIVKEFEE----PAVVAVKHMNPCGVGVGASVYE---------- 289 G Y N+ DA + +VKE PA + KH++P G VG + + Sbjct: 233 GSP-GYINLLDALNSWPLVKELSASLNLPAAASFKHVSPAGAAVGIPLSDVEKQVYFVAD 291 Query: 290 ---------AYQKAYNADPTSIFGGIVAANRIIDADTANMLKDIFLEIVLAPGFTEEALD 340 AY +A AD S FG +A + I+D TA ++ + V+APG+ EAL Sbjct: 292 IENLSPLACAYARARGADRMSSFGDWIALSNIVDVPTAKIISREVSDGVIAPGYEPEALA 351 Query: 341 ILTKKKNIRLLKL----GNLSTAAARKSSFVVT--SIEGGMVVQESDVHSVNPEELQVVT 394 IL+KKK + L + A R+ + VT ++ +S + V Sbjct: 352 ILSKKKGGKYCILQIDPNYVPEAVERRQVYGVTLEQKRNDAIINQSTFKEI------VSQ 405 Query: 395 NRKPTEEELKQLLFGWKVVKHVKSNAIVLAADDMTVGVGAGQMNRVGAAKIAIEQA 450 N+ TE+ + L +K+ +SN++ A + M VG+GAGQ +R+ ++A ++A Sbjct: 406 NKNLTEQAIIDLTVATIAIKYTQSNSVCYARNGMVVGLGAGQQSRIHCTRLAGDKA 461 >CAC2445 [F] COG0138 AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful) Length = 391 Score = 80.1 bits (196), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 69/265 (26%), Positives = 120/265 (45%), Gaps = 35/265 (13%) Query: 212 DLRYGENPHQKAAFYRKPLAAQDTLTSAEQLHGKELSYNNINDANAALQIVKEFEE---- 267 +L+YG NP+Q A + + E L+GK Y N DA + Q+VKE ++ Sbjct: 7 ELKYGCNPNQSPAR----IYVNKGVLPIEVLNGKP-GYINFMDALNSWQLVKELKKATGL 61 Query: 268 PAVVAVKHMNPCGVGVGASVYE------------------AYQKAYNADPTSIFGGIVAA 309 + + KH++P G + + AY +A AD S +G A Sbjct: 62 TSAASFKHVSPAGAATAVPLSDTLKKSYLVEDIDLTPAAIAYSRARGADRMSSYGDFAAI 121 Query: 310 NRIIDADTANMLKDIFLEIVLAPGFTEEALDILTKKKN--IRLLKLGNLSTAAARKSSFV 367 + +D TA +LK + ++AP + +AL+IL KKN ++K+ + Sbjct: 122 SDKVDVATATILKKSVSDGIIAPDYDPKALEILKSKKNGKYNIIKID----PDYEPINLE 177 Query: 368 VTSIEGGMVVQESDVHSVNPEELQ--VVTNRKPTEEELKQLLFGWKVVKHVKSNAIVLAA 425 I G Q+ ++ + L+ V N+ T+E + L+ +K+ +SN++ A Sbjct: 178 RREIYGITFEQKRNITPLTENLLENIVTKNKNLTKEAKRDLIICMITLKYTQSNSVCFAL 237 Query: 426 DDMTVGVGAGQMNRVGAAKIAIEQA 450 D +GVGAGQ +R+ ++A +A Sbjct: 238 DGQVIGVGAGQQSRIHCTRLAASKA 262 Score = 67.8 bits (164), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 27/61 (44%), Positives = 40/61 (65%) Query: 455 KGSVLASDAFFPMGDTLEMAAKAGITAVIQPGGSIKDEESIKVANEYGIAMVFTGVRHFK 514 K L SDAFFP D ++ A+K+G+ ++QPGGSI+D+ I+ + Y + M F+G R F Sbjct: 331 KNVSLGSDAFFPFSDNIDRASKSGVKYIVQPGGSIRDDIVIEACDNYNMIMAFSGTRLFH 390 Query: 515 H 515 H Sbjct: 391 H 391 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.132 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,753,235 Number of extensions: 1569445 Number of successful extensions: 3706 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 3688 Number of HSP's successfully gapped: 5 Length of query: 515 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 404 Effective length of database: 25,576,357 Effective search space: 10332848228 Effective search space used: 10332848228 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)