BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0442.0010.p (204 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA3522 [F] COG0299 Folate-dependent phosphoribosylglycinamide fo... 167 8e-42 MA0316 [F] COG0299 Folate-dependent phosphoribosylglycinamide fo... 126 2e-29 SMa2141 [F] COG0788 Formyltetrahydrofolate hydrolase 91 1e-18 PA4314 [F] COG0788 Formyltetrahydrofolate hydrolase 88 1e-17 RSc1873 [F] COG0788 Formyltetrahydrofolate hydrolase 87 2e-17 >MA3522 [F] COG0299 Folate-dependent phosphoribosylglycinamide formyltransferase PurN Length = 216 Score = 167 bits (424), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 85/197 (43%), Positives = 127/197 (64%), Gaps = 1/197 (0%) Query: 5 RIAVFASGQGSNFAALAEAERAGQLGGGRIELLVSDRPEAPVAKRAEEAGIPALMLRPKD 64 +IAV SG+GSN A+ ++ G + + +++S++ +A +RAE+ GI A+ L P+ Sbjct: 18 KIAVLVSGRGSNLQAIIDSIEKGYIKNAAVSVVISNKADAYALERAEKHGISAVFLDPEG 77 Query: 65 FASRELYEAEIVAELQRRDIGLIVLAGYMRLITPVLLTPYAGRTVNIHPSLLPAFAGKDA 124 R Y+ EI+ L++ D L++LAGY RL+ ++ Y R +NIHPSLLPAF G A Sbjct: 78 -RDRAGYDREILKILKQYDTDLLLLAGYFRLLGSEIIEAYRHRILNIHPSLLPAFKGLHA 136 Query: 125 IDQALDYGVKLTGVTVHFVDGGMDTGPVIAQRSVAVEPGDTVESLAERIHQVEYELYPEV 184 QA +YGVK+ G TVHFVD G+D+GP+I Q+ V V P DT E+L RI + E+ +YPE Sbjct: 137 QKQAFEYGVKVAGCTVHFVDEGLDSGPIIIQKCVPVLPEDTEETLTARILEQEHIIYPEA 196 Query: 185 VRALAEGKVELDGRKTV 201 VR E K++++GR + Sbjct: 197 VRLFVESKLKVEGRNVI 213 >MA0316 [F] COG0299 Folate-dependent phosphoribosylglycinamide formyltransferase PurN Length = 204 Score = 126 bits (316), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 69/196 (35%), Positives = 112/196 (57%), Gaps = 6/196 (3%) Query: 5 RIAVFASGQGSNFAALAEAERAGQLGGGRIELLVSDRPEAPVAKRAEEAGIPALMLRPKD 64 IA+FAS G+N A+ +A R G L G + ++S+ + ++A AG+P L K Sbjct: 10 HIAIFASHTGTNMQAIIDACRRGDLNG-EVCAVISNNSNSQALEKARIAGVPEYHLSNKT 68 Query: 65 FASRELYEAEIVAELQRRDIGLIVLAGYMRLITPVLLTPYAGRTVNIHPSLLPAFAGK-- 122 + + + I L ++ LAGYM+ + P +L Y GR +NIHPSLLP + GK Sbjct: 69 YPEEDELDEAICKVLTESGADIVALAGYMKKLGPEVLKHYKGRILNIHPSLLPKYGGKGM 128 Query: 123 --DAIDQA-LDYGVKLTGVTVHFVDGGMDTGPVIAQRSVAVEPGDTVESLAERIHQVEYE 179 + +A +D G K TGVT+H V+ DTG +I Q + V GDT+++L++R+ + E+ Sbjct: 129 YGTHVHRAVIDAGEKTTGVTIHLVEEEYDTGKIIRQCEIEVLDGDTIDTLSKRVLEREHA 188 Query: 180 LYPEVVRALAEGKVEL 195 Y E ++ ++EG ++L Sbjct: 189 FYVETLKLISEGVIKL 204 >SMa2141 [F] COG0788 Formyltetrahydrofolate hydrolase Length = 86 Score = 90.9 bits (224), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 0/153 (0%), Positives = 0/153 (0%), Gaps = 1/153 (0%) Query: 50 AEEAGIPALMLRPKDFASRELYEAEIVAELQRRDIGLIVLAGYMRLITPVLLTPYAGRTV 109 Sbjct: 133 - 191 Query: 110 NIHPSLLPAFAGKDAIDQALDYGVKLTGVTVHFVDGGMDTGPVIAQRSVAVEPGDTVESL 169 Sbjct: 192 251 Query: 170 AERIHQVEYELYPEVVRALAEGKVELDGRKTVI 202 Sbjct: 252 284 >PA4314 [F] COG0788 Formyltetrahydrofolate hydrolase Length = 86 Score = 87.8 bits (216), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 0/210 (0%), Positives = 0/210 (0%), Gaps = 12/210 (5%) Query: 1 MQW--------SRIAVFASGQGSNFAALAEAERAGQLGGGRIELLVSDRPEAPVAKRAEE 52 + Sbjct: 76 PEIGTVVVSES--- 132 Query: 53 AGIPALMLRPKDFASRELYEAEIVAELQRRDIGLIVLAGYMRLITPVLLTPYAGRTVNIH 112 Sbjct: 133 - 191 Query: 113 PSLLPAFAGKDAIDQALDYGVKLTGVTVHFVDGGMDTGPVIAQRSVAVEPGDTVESLAER 172 Sbjct: 192 251 Query: 173 IHQVEYELYPEVVRALAEGKVELDGRKTVI 202 Sbjct: 252 281 >RSc1873 [F] COG0788 Formyltetrahydrofolate hydrolase Length = 86 Score = 86.7 bits (213), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 0/213 (0%), Positives = 0/213 (0%), Gaps = 15/213 (7%) Query: 1 MQWS--------RIAVFASGQGSNFAALAEAERAGQLGGGRIELLVSDRPEAPVAKRAEE 52 + Sbjct: 78 IGTV--- 134 Query: 53 AGIPAL---MLRPKDFASRELYEAEIVAELQRRDIGLIVLAGYMRLITPVLLTPYAGRTV 109 Sbjct: 135 - 193 Query: 110 NIHPSLLPAFAGKDAIDQALDYGVKLTGVTVHFVDGGMDTGPVIAQRSVAVEPGDTVESL 169 Sbjct: 194 253 Query: 170 AERIHQVEYELYPEVVRALAEGKVELDGRKTVI 202 Sbjct: 254 286 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,750,120 Number of extensions: 723703 Number of successful extensions: 3323 Number of sequences better than 1.0e-05: 38 Number of HSP's gapped: 3312 Number of HSP's successfully gapped: 38 Length of query: 204 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 103 Effective length of database: 26,729,697 Effective search space: 2753158791 Effective search space used: 2753158791 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)