BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0442.0004.p (303 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll2603 [F] COG0152 Phosphoribosylaminoimidazolesuccinocarboxami... 147 2e-35 CC3242 [F] COG0152 Phosphoribosylaminoimidazolesuccinocarboxamid... 143 4e-34 AGc4615 [F] COG0152 Phosphoribosylaminoimidazolesuccinocarboxami... 135 1e-31 >mll2603 [F] COG0152 Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase Length = 337 Score = 147 bits (371), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 102/296 (34%), Positives = 155/296 (52%), Gaps = 31/296 (10%) Query: 23 PLLYKGKVRELYDLGE-EVLIVVTDRISAFDYVLDPAVPEKGNVLNRLSAFWFGQTSELM 81 P YKGKVRE YDL + +I+ TDR+SAFD +L ++P KG +L + + +WF +T+++ Sbjct: 37 PGYYKGKVRENYDLADGRRIIIATDRLSAFDIILT-SIPFKGEILTQTARYWFEETADIC 95 Query: 82 ENHVVHIDVDRLGGIVKDREALRNRIMVVRKAERIDIECVVRGCITGGG-----WRQYQE 136 NHV+ E ++V + + + +E VVRG + G R + Sbjct: 96 PNHVL--------------EYPDPNVVVGTRLDILPVEIVVRGYLAGTTSTSILTRYRRG 141 Query: 137 TGKVNGIGLPAGLRKNAQLSQPIFTPAAK-NDVGHDEDIPFEKM--QELIGXXXXXXXXX 193 ++ G+ LP GLR N +L+ P+ TP +K D GHDE + ++ Q L+ Sbjct: 142 EREMYGMRLPDGLRDNEKLAAPVITPTSKAADGGHDEPLSRAEILAQGLLTQAQWDTVSD 201 Query: 194 XXXXXXXXXREYCEERGIILADCKFEFGL-LDGKVILIDEIFTPDASRFWAKDKY----A 248 + ERG+ILAD K+EFG +G +IL DEI TPD+SR+W Y A Sbjct: 202 YALKLFARGQARAAERGLILADTKYEFGTDKNGTIILADEIHTPDSSRYWIAASYEQALA 261 Query: 249 LDIEIDSMDKEPVRTYLSASSWDKNSTPDP-LPAEVVEETTRRYLDIYHRLTGKTL 303 DS DK+ +R++++A D P P +P E+VE+ +R Y Y +TGK Sbjct: 262 SGTRPDSFDKDFIRSWVAARC-DPYKDPIPRIPDEIVEQASRIYAQAYEAITGKAF 316 >CC3242 [F] COG0152 Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase Length = 320 Score = 143 bits (360), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 103/318 (32%), Positives = 153/318 (48%), Gaps = 43/318 (13%) Query: 7 VMTYPAVSTAVDLVNAPLLYKGKVRELYDLGE-EVLIVVTDRISAFDYVLDPAVPEKGNV 65 ++ P VDL P Y+GKVR+ YDL + +IV +DR+SAFD L AVP KG V Sbjct: 2 TLSAPNALAVVDLPCLPNHYRGKVRDNYDLPDGRRVIVASDRLSAFDRNLC-AVPGKGQV 60 Query: 66 LNRLSAFWFGQTSELMENHVVHIDVDRLGGIVKDREALRNRIMVVRKAERIDIECVVRGC 125 L + FWF T+++ NHVV +++ ++ + + +E VVRG Sbjct: 61 LTETARFWFDATADICPNHVVAYP--------------DPNVLIGKRLKMLPVELVVRGY 106 Query: 126 ITGG-----------GWRQYQETGKVNGIGLPAGLRKNAQLSQPIFTPAAKNDVGHDEDI 174 + G G RQ + G+ LP GL+ N +L Q I TP +K G D D Sbjct: 107 LAGTTSTSILTLYNKGERQ------MYGVTLPDGLKANQRLPQAILTPTSKAADG-DHDA 159 Query: 175 PFEK----MQELIGXXXXXXXXXXXXXXXXXXREYCEERGIILADCKFEFGLLD-GKVIL 229 P Q L+ + ERG+ILAD K+EFGL + G ++L Sbjct: 160 PLSPDEVLAQGLVTPAQWEQLSAYALALFARGQAMAAERGLILADTKYEFGLDETGAIVL 219 Query: 230 IDEIFTPDASRFWAKDKYALDIEI----DSMDKEPVRTYLSASSWDKNSTPDPLPAEVVE 285 DEI TPD+SRFW Y E +S DK+ VR +++ N + +PAE++ Sbjct: 220 ADEIHTPDSSRFWKAATYEARFEAGQRPESFDKDFVRAWVAERCDPYNDSIPEIPAELIA 279 Query: 286 ETTRRYLDIYHRLTGKTL 303 + + Y++ + +TG+T Sbjct: 280 QASAVYIEAFEAITGQTF 297 >AGc4615 [F] COG0152 Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase Length = 466 Score = 135 bits (339), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 20/293 (6%), Positives = 43/293 (14%), Gaps = 29/293 (9%) Query: 23 PLLYKGKVRELYDLGE-EVLIVVTDRISAFDYVLDPAVPEKGNVLNRLSAFWFGQTSELM 81 L G+ E L+ + A + + EL Sbjct: 13 NLAAFGRKELDIAETEMPGLMSCRKEFGESKPLKG-ARITGSLHMTIQTGVLIETLKELG 71 Query: 82 ENHVVHIDVDRLGGIVKDREALRNRIMVVRKAERIDIECVVRGCI---TGGGWRQYQETG 138 A I + + + + +Y + Sbjct: 72 AEIRWASC--------------NIFSTQDHAAAAIAAAGIPVFAVKGESLTEYWEYTDKI 117 Query: 139 --KVNGIGLPAGLRKNAQLSQPIFTPAAKN-DVGHDEDI-PFEKMQE-LIGXXXXXXXXX 193 +G L + I A + E+ Sbjct: 118 FQWTDGGLSNMILDDGGDATMYILLGARAEAGEDVLSNPGSEEEEILFAQINKRLKASPG 177 Query: 194 XXXXXXXXXREYCEERGIILADCKFEFGL-LDGKVILIDEIFTPDASRFWAKDKYALDI- 251 + EE + L + + + Sbjct: 178 WFTKQRDALKGVTEETTTGVHRLYDLAKKGLLPFPAINVNDSVTKSKFDNKYGCKESLVD 237 Query: 252 ---EIDSMDKEPVRTYLSASSWDKNSTPDPLPAEVVEETTRRYLDIYHRLTGK 301 + + + L I Sbjct: 238 GIRRATDVMMAGKVAVVCGYGDVGKGSAASLQGAGARVKVTEIDPICALQAAM 290 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.140 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,544,105 Number of extensions: 923263 Number of successful extensions: 1443 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 1426 Number of HSP's successfully gapped: 3 Length of query: 303 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 197 Effective length of database: 26,153,027 Effective search space: 5152146319 Effective search space used: 5152146319 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)