BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0440.0010.p (379 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_licC [G] COG1455 Phosphotransferase system cellobiose-specifi... 341 1e-93 BS_ywbA [G] COG1455 Phosphotransferase system cellobiose-specifi... 315 6e-86 SP0474 [G] COG1455 Phosphotransferase system cellobiose-specific... 270 2e-72 lin0900 [G] COG1455 Phosphotransferase system cellobiose-specifi... 228 9e-60 BS_ydhO [G] COG1455 Phosphotransferase system cellobiose-specifi... 223 3e-58 >BS_licC [G] COG1455 Phosphotransferase system cellobiose-specific component IIC Length = 452 Score = 341 bits (875), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 181/379 (47%), Positives = 249/379 (65%), Gaps = 14/379 (3%) Query: 1 MSKIQTFMEEKVAPFAGRIGQVKQLVAIRDGISYIMPLIIIGSIALILNSLPIEANWYLN 60 M+K+ +EEKV P AGRI + L A+RDGI MPLIIIGS LI+ +LPI Y Sbjct: 1 MNKVNQILEEKVMPIAGRIAGQRHLQALRDGIILTMPLIIIGSFFLIIGNLPIPG--YAE 58 Query: 61 LMNE---SGWGARLGMIVDGTFGLIGILAAFTIAYSLANQYSLDGMSVGILSLSSYLLST 117 M + S W +L VD TF ++G++AAF IAY LA +Y +D +S G +SL+++LL+T Sbjct: 59 FMAKTFGSSWSEKLAYPVDATFEIMGLVAAFGIAYRLAEKYGVDALSAGAISLAAFLLAT 118 Query: 118 PNILS--ADNAA-------GIPLSLMGSKGLFVAIIIGIISTEIFRFVISKNLVIKLPDG 168 P + D A GIPLSLMGSKGLFVA+II ++STEI+R +I +NLV K+PDG Sbjct: 119 PYQVPFMPDGATKEIMVGGGIPLSLMGSKGLFVAMIIAMVSTEIYRLIIQRNLVFKMPDG 178 Query: 169 VPPAVGRSFTALIPGFFIIVFFAAVSWILDATGIGSLHDLIQTVLGKPLGLLGGTIFGAI 228 VPPAV +SF ALIPGF +I A I++AT SLH+++ +LG PL +LGG++ G++ Sbjct: 179 VPPAVSKSFVALIPGFAVIFLIWAARLIVEATPFESLHNIVSVLLGTPLSILGGSLGGSL 238 Query: 229 IYDLLISLFWLTXXXXXXXXXXXXXXXWMQKMDENRLALQAGKELPNIFTQPFFDIFVHM 288 + + + L W W MD NR+A QAG+ELP IFTQ FFDI+V++ Sbjct: 239 VAEAVKMLLWACGLHGANIVGGVMAPIWYGAMDANRIAFQAGEELPKIFTQQFFDIWVNI 298 Query: 289 GGAGTIFALAVCLFLFSKSNQNKSMGRLSIMPSAFNIGEPIMFGVPVVLNPIMFIPFILA 348 GG+G AL V +FL ++S Q K +G+L++ P+ FNI EPI+FG+P+V+NP++ +PFI+ Sbjct: 299 GGSGATLALVVTMFLRARSKQMKQLGKLAVGPAIFNINEPIIFGMPIVMNPMLLLPFIIT 358 Query: 349 PIAMVITTYFAMVTGLVPK 367 P+ V TY M TGLV K Sbjct: 359 PLVTVTLTYIGMSTGLVAK 377 >BS_ywbA [G] COG1455 Phosphotransferase system cellobiose-specific component IIC Length = 444 Score = 315 bits (808), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 167/379 (44%), Positives = 242/379 (63%), Gaps = 14/379 (3%) Query: 1 MSKIQTFMEEKVAPFAGRIGQVKQLVAIRDGISYIMPLIIIGSIALILNSLPIEANWYLN 60 MS MEEK+ P AG+I + L A+RDGI MPLIIIGS+ LIL SLPI Y + Sbjct: 1 MSTFTRVMEEKIMPVAGKIAGQRHLSALRDGIILTMPLIIIGSVFLILTSLPIPG--YAD 58 Query: 61 LMNE---SGWGARLGMIVDGTFGLIGILAAFTIAYSLANQYSLDGMSVGILSLSSYLLST 117 M + W +LG V+ +F ++ ++AAF IAY LA Y +D +S G +S++++LL+T Sbjct: 59 FMASVFGNEWADKLGYPVNASFDIMAMIAAFGIAYRLAESYGVDALSAGAISIAAFLLAT 118 Query: 118 P---------NILSADNAAGIPLSLMGSKGLFVAIIIGIISTEIFRFVISKNLVIKLPDG 168 P + S GIP++L+GSKGLFVA++I + STEI+R++I KN+V K+PDG Sbjct: 119 PFEVPFTPHGSTESIMVGGGIPITLLGSKGLFVAMLIALFSTEIYRYIIQKNIVFKMPDG 178 Query: 169 VPPAVGRSFTALIPGFFIIVFFAAVSWILDATGIGSLHDLIQTVLGKPLGLLGGTIFGAI 228 VPPAV +SF ALIPGF I++ +++ T SLH+++ +LG PL +LGG++ G++ Sbjct: 179 VPPAVSKSFVALIPGFIIVLLVWLARLLIEMTPFQSLHNVVGDLLGTPLSILGGSLGGSL 238 Query: 229 IYDLLISLFWLTXXXXXXXXXXXXXXXWMQKMDENRLALQAGKELPNIFTQPFFDIFVHM 288 I + + L W W MD NRLA QAG+ LP+IFT FF I++++ Sbjct: 239 IAEFVQMLLWSCGIHGASIIGGIMAPIWYGAMDANRLAFQAGEALPSIFTTQFFQIWINV 298 Query: 289 GGAGTIFALAVCLFLFSKSNQNKSMGRLSIMPSAFNIGEPIMFGVPVVLNPIMFIPFILA 348 GG+G AL + + + S+S Q K +GRL I P+ FNI EPI+FG+P+V+NP++ +PFI+A Sbjct: 299 GGSGATLALVLTMLVRSRSKQMKQLGRLGIGPALFNINEPIIFGMPIVMNPLLIVPFIIA 358 Query: 349 PIAMVITTYFAMVTGLVPK 367 P+ + TY M TGLV + Sbjct: 359 PLLTITATYIGMSTGLVAR 377 >SP0474 [G] COG1455 Phosphotransferase system cellobiose-specific component IIC Length = 440 Score = 270 bits (691), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 159/380 (41%), Positives = 221/380 (58%), Gaps = 14/380 (3%) Query: 1 MSKIQTFMEEKVAPFAGRIGQVKQLVAIRDGISYIMPLIIIGSIALILNSLPIEANWYLN 60 M+ I F+E KVAPF ++G + L AIR+G MPLI++GS+ LIL S P EA + N Sbjct: 1 MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSLFLILISWPQEA--FTN 58 Query: 61 LMNESGWGARLGMIVDGTFGLIGILAAFTIAYSLANQYSLDGMSVGILSLSSYLLSTPNI 120 +N G + L + T +I ++A F IAY L+ Y DG S GI++LSS++L P Sbjct: 59 WLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPSAGIIALSSFVLMAPRF 118 Query: 121 LSA--DN---------AAGIPLSLMGSKGLFVAIIIGIISTEIFRFVISKNLVIKLPDGV 169 S D IP S + + LF+AI IG+++ EI+R I + + IK+P GV Sbjct: 119 SSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAEIYRMFIQRGITIKMPSGV 178 Query: 170 PPAVGRSFTALIPGFFIIVFFAAVSWILDATGI-GSLHDLIQTVLGKPLGLLGGTIFGAI 228 P V +SF+AL+ GF V +A V L+A G+ G L+ L+ ++G PL L+ GT+ G I Sbjct: 179 PDVVSKSFSALLSGFTTFVLWALVLKGLEAAGVAGGLNGLLGAIVGTPLKLIAGTLPGMI 238 Query: 229 IYDLLISLFWLTXXXXXXXXXXXXXXXWMQKMDENRLALQAGKELPNIFTQPFFDIFVHM 288 + ++ S FW W+Q EN+ A+ AG+ L +I T PF D+FV + Sbjct: 239 LCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEAVAAGQTLQHIITLPFKDLFVFI 298 Query: 289 GGAGTIFALAVCLFLFSKSNQNKSMGRLSIMPSAFNIGEPIMFGVPVVLNPIMFIPFILA 348 GG G LA+CLFLFSKS NK++G+L+I+PS FNI I+F P VLNPIM IPFI Sbjct: 299 GGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFNINTAILFTFPTVLNPIMLIPFIAT 358 Query: 349 PIAMVITTYFAMVTGLVPKT 368 P + TY +M GLVP T Sbjct: 359 PTINALITYVSMAVGLVPYT 378 >lin0900 [G] COG1455 Phosphotransferase system cellobiose-specific component IIC Length = 301 Score = 228 bits (582), Expect = 9e-60, Method: Compositional matrix adjust. Identities = 0/371 (0%), Positives = 0/371 (0%), Gaps = 10/371 (2%) Query: 1 MSKIQTFMEEKVAPFAGRIGQVKQLVAIRDGISYIMPLIIIGSIALILNSLPIEANWYLN 60 MS +++ +G+ L + I + I+ A Sbjct: 1 MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAY---- 56 Query: 61 LMNESGWGARLGMIVDGTFGLIGILAAFTIAYSLANQYSLDGMSVGILSLSSYLLSTPNI 120 L M + AA + + + + L Sbjct: 57 -QAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREG 115 Query: 121 LSADN----AAGIPLSLMGSKGLFVAIIIGIISTEIFRFVISKNLVIKLPDGVPPAVGRS 176 + + + +I L + + Sbjct: 116 KAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPE 175 Query: 177 FTALIPGFFIIVFFAAVSWILDATGIGSLHDLIQTVLGKPLGLLGGTIFGAIIYDLLISL 236 T P +I + + + YD+ + Sbjct: 176 ATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLI 235 Query: 237 FWLTXXXXXXXXXXXXXXXWMQKMDENRLALQAGKELPNIFTQPFFDIF-VHMGGAGTIF 295 ++ + + + ++ A Sbjct: 236 LVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSL 295 Query: 296 ALAVCLFLFSKSNQNKSMGRLSIMPSAFNIGEPIMFGVPVVLNPIMFIPFILAPIAMVIT 355 Sbjct: 296 355 Query: 356 TYFAMVTGLVP 366 Sbjct: 356 366 >BS_ydhO [G] COG1455 Phosphotransferase system cellobiose-specific component IIC Length = 271 Score = 223 bits (569), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 0/368 (0%), Positives = 0/368 (0%), Gaps = 10/368 (2%) Query: 8 MEEKVA----PFAGRIGQVKQLVAIRDGISYIMPLIIIGSIALILNSLPIEANWYLNLMN 63 M A + A Sbjct: 1 MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKADKKKST-ATTKKTQKT 59 Query: 64 ESGWGARLGMIVDGTFGLIGILAAFTIAYSLANQYSLDGMSVGILSLSSYLLSTPNILSA 123 ++ + + ++++++ + + Sbjct: 60 AKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASASFTEKCTKRKGYKSHCV 119 Query: 124 DNAAGI----PLSLMGSKGLFVAIIIGIISTEIFRFVISKNLVIKLPDGVPPAVGRSFTA 179 + + + + +I + + V ++ Sbjct: 120 KVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGGSSPRTGFDCSGLVYYAYKD 179 Query: 180 LIPGFFIIVFFAAVSWILDATGIGSLHDLIQTVLGKPLGLLGGTIFGAIIYDLLISLFWL 239 L+ A S V + G G + + Sbjct: 180 LVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQGRGTADHVGVYVGNGKFIQSPR 239 Query: 240 TXXXXXXXXXXXXXXXWMQKMDENRLALQAGKELPNIFTQPFFDIF-VHMGGAGTIFALA 298 Sbjct: 240 299 Query: 299 VCLFLFSKSNQNKSMGRLSIMPSAFNIGEPIMFGVPVVLNPIMFIPFILAPIAMVITTYF 358 Sbjct: 300 359 Query: 359 AMVTGLVP 366 Sbjct: 360 367 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.145 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,514,594 Number of extensions: 1060372 Number of successful extensions: 3352 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 3271 Number of HSP's successfully gapped: 28 Length of query: 379 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 271 Effective length of database: 25,922,359 Effective search space: 7024959289 Effective search space used: 7024959289 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)