BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0437.0034.p (440 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CC2672 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 165 1e-40 BH2935 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 155 2e-37 CC0300 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 135 1e-31 CC3125 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 134 4e-31 SSO2527 [Q] COG1228 Imidazolonepropionase and related amidohydro... 130 6e-30 >CC2672 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 429 Score = 165 bits (418), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 130/412 (31%), Positives = 204/412 (49%), Gaps = 48/412 (11%) Query: 27 ILIRDGLIQEIGKASETAIPSHYQAIDVEGKYVMPGLINAHVHLFADGKPFTLSASEGLL 86 +++ DG I IGK + A+P+ A+D+ G ++PGLI+ HVHL + L Sbjct: 51 VIVTDGRITSIGKKGD-AVPAGATAVDLPGVTLLPGLIDMHVHL------------DSLA 97 Query: 87 DFAFHHMLD-TRLGRSVLKKRMKRNALTALHSGVTTMRSVGEFFYTDVQLRDEIKANQFV 145 + ++ L+ + SV++ NA L +G TT+R+VG Y DV LR+ I A Sbjct: 98 EVGGYNSLEYSDRFWSVVQTA---NAKKTLEAGFTTVRNVGAADYDDVGLREAIDAGYVP 154 Query: 146 GPNLLVSGYFLSVTGGHG-----APYL----ALVGDSPWEARRNVRINVKNGVDLIKICV 196 GP ++ + TGGH P + DSP EAR+ VR K G +IKIC Sbjct: 155 GPRIVTAAISFGATGGHCDSTFFPPSMDQKNPFNSDSPDEARKAVRTLKKYGAQVIKICA 214 Query: 197 TGGVTDAKMAGEAGRLQMTEEEVAAICEEAHKIGMRVAAHVESTEGVRIALKGGVDTIEH 256 TGGV E G+ Q+T EE+ A+ +EAH G++VAAH G+R A++ GVDTIEH Sbjct: 215 TGGVF--SRGNEPGQQQLTYEEMKAVVDEAHMAGIKVAAHAHGASGIREAVRAGVDTIEH 272 Query: 257 GAEMDEEIIRLYQNNPRALKGYTALIPTLQAAYPSAKLDLSLTKVSETVKENSRRVYDS- 315 + +D+E I+L KG + Y A+ K + +++N R+ D Sbjct: 273 ASLVDDEGIKLAVQ-----KGAYFSMDIYNTDYTQAE-----GKKNGVLEDNLRKDRDIG 322 Query: 316 --MLKGVRQAVEHNIKIGVGTDAAMPYVTHYDLWRELDHFMR--QTKLNSRRIIEQVTKS 371 + R+A++ +K+ GTDA + H D ++ +R T L + I+ T + Sbjct: 323 ELQRENFRKALKAGVKMVYGTDAGI--YPHGDNAKQFAVMVRYGATPLQA---IQSATLT 377 Query: 372 NAEILGIGHLTGSIDIGKQADLIVLEQNPLDNIQALADIAMVMVRGNLIQTP 423 AE LG G + +G+ D+I + +PL ++ L VM G +++ P Sbjct: 378 AAEALGRSKDVGQVAVGRYGDMIAVAGDPLADVTTLEKPVFVMKGGAVVKAP 429 >BH2935 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 394 Score = 155 bits (391), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 132/416 (31%), Positives = 190/416 (45%), Gaps = 36/416 (8%) Query: 5 YAFKNCNVIYGDAGRDVAAHMTILIRDGLIQEIGKASETAIPSHYQAIDVEGKYVMPGLI 64 Y N +I G A R + + +D ++ K S +I ID V+PGLI Sbjct: 3 YCIHNTRLIIGIADRVIENGSILFDQDKIL----KVSAHSITDADVFIDGTTFSVLPGLI 58 Query: 65 NAHVHLFADGKPFTLSASEGLLDFAFHHMLDTRLGRSVLKKRMKRNALTALHSGVTTMRS 124 + HVHL D E D AF + + G L L +G+TT+R+ Sbjct: 59 DVHVHLGMDCSVDPFDTMENTED-AFTSFIAYKQG------------LQFLRAGITTVRN 105 Query: 125 VGEFFYTDVQLRDEIKANQFVGPNLLVSGYFLSVTGGHGAPYLALVGDSPWEARRNVRIN 184 +G D+ + +++ +GP + +G L +TGGHG P V D E + R Sbjct: 106 LGTKNNVDIIYKKAVESGLVIGPRVYAAGQPLIMTGGHGQPMGTEV-DGKDEVIKATRQR 164 Query: 185 VKNGVDLIKICVTGGVTDAKMAGEAGRLQMTEEEVAAICEEAHKIGMRVAAHVESTEGVR 244 +K G DLIKI TGGV G + +E+ IC+EA K + AAH GV+ Sbjct: 165 IKEGADLIKIMATGGVLTK--GNHPGAVHFNVDELKCICQEAAKASLTTAAHAIGLNGVK 222 Query: 245 IALKGGVDTIEHGAEMDEEIIRLYQNNPRALKGY---TALIPTLQAAYPSAKLDLSLTKV 301 A+K GV+TIEHG +D+E + + R L Y T L PTL + K+ Sbjct: 223 NAIKAGVNTIEHGYYLDKETV----DTMRDLGIYLTPTLLAPTLLQKNSEHIPQHMIDKI 278 Query: 302 SETVKENSRRVYDSMLKGVRQAVEHNIKIGVGTDAAMPYVTHYDLWRELDHFMRQTKLNS 361 E KE+ K R A+E +KI GTDA P+ L EL M + + + Sbjct: 279 EEIEKEHR--------KSFRLALERGVKIIAGTDAGTPFNNPGQLVEEL-KIMLEEGMTT 329 Query: 362 RRIIEQVTKSNAEILGIGHLTGSIDIGKQADLIVLEQNPLDNIQALADIAMVMVRG 417 + IE T A+ L + L GS++ K ADLIV++ NPL++I L +I V G Sbjct: 330 AQAIESATYLGAKCLKVDSLIGSLEEQKLADLIVVKGNPLEDISNLKNIQHVFKGG 385 >CC0300 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 428 Score = 135 bits (340), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 120/406 (29%), Positives = 183/406 (45%), Gaps = 51/406 (12%) Query: 29 IRDGLIQEIGKASETAIPSHYQAIDVEGKYVMPGLINAHVHLFADGKPFTLSASEGLLDF 88 I DG + E G + +D++ +V+PGLI++HVHL P G L Sbjct: 58 ILDGYVAEPGG----------KVVDLKDSFVLPGLIDSHVHLTGQQNP------NGRLQA 101 Query: 89 AFHHMLDTRLGRSVLKKRMKRNALTALHSGVTTMRSVGEFFYTDVQLRDEIKANQFVGPN 148 D+ + + ++ L +G TT+ ++G LR+ +K GP Sbjct: 102 VTQSPSDSAMVGAGFARKT-------LMAGFTTVANLGANSQAIFALREGVKRGDVAGPR 154 Query: 149 LLVSGYFLSVTGGHG-----------APYLALVGDSPWEARRNVRINVKNGVDLIKICVT 197 +L SG +SV GGHG A V + RR VR + G DLIKI T Sbjct: 155 ILASGSSISVHGGHGDINGYSEDVMHALRPESVCSGADDCRRAVREQIWLGADLIKITAT 214 Query: 198 GGVTDAKMAGEAGRLQMTEEEVAAICEEAHKIGMRVAAHVESTEGVRIALKGGVDTIEHG 257 GGV AG A Q ++ E+AAI E AH +G +V AH +G+ LK G D+IEHG Sbjct: 215 GGVLSNTAAGLA--KQFSDAELAAIVESAHSMGRKVTAHAHGGDGINAFLKAGGDSIEHG 272 Query: 258 AEMDEEIIRLYQNNPRALKGYTALIPTLQAA---YPSAKLDLSLTKVSETVKENSRRVYD 314 +D E I L + K L+PTL A Y A + ++T K + Sbjct: 273 TYLDNEGIALMK------KSGAYLVPTLMAGDFVYRIASGPNNYFTPAQTAK--ALEAGP 324 Query: 315 SMLKGVRQAVEHNIKIGVGTDAAMPYVTHYDLWRELDHFMRQTKLNSRRIIEQVTKSNAE 374 ML VR+A +K+ GTD+ + H D E + + + I+ T + A+ Sbjct: 325 KMLDMVRRAHADGVKVAFGTDSGVS--AHGDNAGEF-ALLVKAGMTPLEAIQTATVNAAD 381 Query: 375 ILGIGHLTGSIDIGKQADLIVLEQNPLDNIQALADIAMVMVRGNLI 420 + + GS+ GK ADLI ++ +PL ++ L + V ++G ++ Sbjct: 382 HFSLSNEIGSLTPGKAADLIAVKGDPLKDVTELQRVTSV-IKGGVV 426 >CC3125 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 431 Score = 134 bits (336), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 117/400 (29%), Positives = 181/400 (45%), Gaps = 60/400 (15%) Query: 46 PSHYQAIDVEGKYVMPGLINAHVHLFADGK------PFTLSASEGLLDFAFHHMLDTRLG 99 P + D G V+PGLI+ H HL D + P SA++ A H Sbjct: 66 PKDAKVTDWSGLTVLPGLIDMHTHLVDDEQSENIALPLLRSAAQQAYIGAGH-------- 117 Query: 100 RSVLKKRMKRNALTALHSGVTTMRSVGEF-FYTDVQLRDEIKANQFVGPNLLVSGYFLSV 158 A T L +G T++R VG + D LRD I GP + V+G +++ Sbjct: 118 -----------ARTTLMAGFTSVRDVGTWRALGDAALRDAITEGLVPGPRMSVAGAYVTA 166 Query: 159 TGGHG-----APYLALVGD-------SPWEARRNVRINVKNGVDLIKICVTGGVTDAKMA 206 GG G AP + + D + R VR + G D IK+ TG V Sbjct: 167 PGGGGEITGVAPDVTIPADMRRGVVEDAADVHRKVRALLVGGADFIKLIATGAVLTEGT- 225 Query: 207 GEAGRLQMTEEEVAAICEEAHKIGMRVAAHVESTEGVRIALKGGVDTIEHGAEMDEEIIR 266 E G+L+++EEE+ A EEA K G V AH EG++IA++ GV +IEHG+ +D+E I Sbjct: 226 -EPGQLELSEEEIRAAVEEAAKRGTYVTAHAHGAEGIKIAVRAGVRSIEHGSLIDDEGIA 284 Query: 267 LYQNNPRALKG--YTA-LIPTL--QAAYPSAKLDLSLTKVSETVKENSRRVYDSMLKGVR 321 L + L Y I T + +P+ + +R D+ +G R Sbjct: 285 LMKAKGAFLVADIYNGDFIDTYGREHGWPAGMI------------RKNRETTDAQREGFR 332 Query: 322 QAVEHNIKIGVGTDAAMPYVTHYDLWRELDHFMRQTKLNSRRIIEQVTKSNAEILGIGHL 381 +AV+ +KI GTDA + Y ++ R++ + +R + + I+ T AE++G Sbjct: 333 KAVKAGVKIAYGTDAGV-YPHGWNA-RQMPYMVRY-GMTPMQAIQSATTVAAELMGKTGQ 389 Query: 382 TGSIDIGKQADLIVLEQNPLDNIQALADIAMVMVRGNLIQ 421 G G ADLI + +PL ++ L +A VM G +++ Sbjct: 390 VGCHAPGCYADLIAVAGDPLADVSVLTKVAKVMKGGTVVK 429 >SSO2527 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 407 Score = 130 bits (326), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 128/437 (29%), Positives = 205/437 (46%), Gaps = 64/437 (14%) Query: 6 AFKNCNVIYGDAGRDVAAHMTILIRDGLIQEIGKASETAIPSHYQAIDVEGKYVMPGLIN 65 KN NV G+ V + I ++DG I EI K ++ A ID + +VMPGLI+ Sbjct: 4 GIKNGNVF---NGKGVVGKVNIYVKDGKIVEISKENKQA----KIEIDAKDMFVMPGLID 56 Query: 66 AHVHLFADGKPFTLSASEGLLDFAFHHMLDTRLGRSVLKKRMKRNALTALHSGVTTMRSV 125 AH+HL T LL F + R+ R+ K +++ L +G TT+R Sbjct: 57 AHIHL-------TGIKGGSLLKTMFEKP-EYRVLRA--SKWLEK----LLLAGFTTVRDC 102 Query: 126 GEFFYTDVQLRDEIKANQFVGPNLLVSGYFLSVTGGHG------------APYLALVGDS 173 GE + L+ + GP ++ +G +S T GHG A + D Sbjct: 103 GETI--SLALKRGVSEGIINGPRIIAAGKPISQTFGHGELSHDVPLEFSMALSFSEFCDG 160 Query: 174 PWEARRNVRINVKNGVDLIKICVTGGVTDAKMAGEAGRLQMTEEEVAAICEEAHKIGMRV 233 R R +++G D IKI TGGV + E Q++ EE++AI EA KI V Sbjct: 161 VESCIRASRKVLRDGADFIKIFATGGVLSQRDRPE--HPQLSYEEISAIVNEAEKINTYV 218 Query: 234 AAHVESTEGVRIALKGGVDTIEHGAEMDEEIIRLYQNNPRALKGYTALIPTL---QAAYP 290 AAH +G RIA++ G+ T+EHG + +E ++L + L PTL + Y Sbjct: 219 AAHAHGDKGARIAVEAGIKTLEHGTLLRDETLKLMRERN------VTLTPTLTIQELIYK 272 Query: 291 SAKL----DLSLTKVSETVKENSRRVYDSMLKGVRQAVEHNIKIGVGTDAAMPY-VTHYD 345 K + L K++E V +S++ V++A E+ + I GTD + D Sbjct: 273 YGKQIGVDEWGLQKINE--------VRESVVNVVKKAEEYGVMIITGTDLGFETGLEEID 324 Query: 346 L---WRELDHFMRQTKLNSRRIIEQVTKSNAEILGIGHLTGSIDIGKQADLIVLEQNPLD 402 + W E + + L++ + T ++A +GI G+I++GK AD+IV++ +P + Sbjct: 325 IGKNWMESVLLVERGGLSTISSLRASTYNSAFAIGIN--AGAIEVGKDADIIVIDGDPTE 382 Query: 403 NIQALADIAMVMVRGNL 419 NI+ L+ + V+ G L Sbjct: 383 NIRDLSKVRRVVKNGKL 399 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,292,940 Number of extensions: 1369954 Number of successful extensions: 13029 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 13017 Number of HSP's successfully gapped: 9 Length of query: 440 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 331 Effective length of database: 25,807,025 Effective search space: 8542125275 Effective search space used: 8542125275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)