BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0437.0028.p (531 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2728 [T] COG4753 Response regulator containing CheY-like recei... 92 1e-18 BH3679 [T] COG4753 Response regulator containing CheY-like recei... 89 2e-17 BH3842 [T] COG4753 Response regulator containing CheY-like recei... 83 1e-15 Z3647 [K] COG2207 AraC-type DNA-binding domain-containing proteins 82 2e-15 BH2109 [T] COG4753 Response regulator containing CheY-like recei... 81 4e-15 >BH2728 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 510 Score = 92.4 bits (228), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 43/104 (41%), Positives = 62/104 (59%) Query: 418 DQGEVVITECLSWIREHTKEEVTLERAADYFHFNPSYFSTLFKSRTGRTFSEHVTAVRMK 477 ++G I + L++I EH E ++L + A+YFHFNPSY S F+ FSE++ VR++ Sbjct: 396 NEGSSSIQKMLAYIEEHHSEPLSLSQLANYFHFNPSYLSAYFRQHHDEGFSEYLNKVRIR 455 Query: 478 RAKELLAEDKLRIYEISVECGFQDPKYFCRVFKKYHSMSPKTYK 521 +AKE L + I EI G+ D YFC+VFKK M P Y+ Sbjct: 456 KAKEWLKSSTIPISEIYALVGYSDHSYFCKVFKKRTGMPPSRYR 499 Score = 69.7 bits (169), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 35/104 (33%), Positives = 62/104 (59%), Gaps = 1/104 (0%) Query: 2 RILIVDDEPRHLRGMVNLIHRLRPEDQ-VAVAKDGLAAMELAKSHRPEAILTDIRMPGMD 60 +LIVDDE +G++N + Q V A +G A+EL + P ++TDI MP MD Sbjct: 3 NVLIVDDEKLIRQGIINFFDWEKEGFQIVGEAANGKDALELISKNEPHIVMTDIVMPVMD 62 Query: 61 GLEFLEWLKLEGIKAKVVMVSAYNLFEYAQTAVRHGAYDYLLKP 104 G++ ++ +K + + +++++S++ FEY ++ G DY+LKP Sbjct: 63 GIDLVKRVKRDFPEVEIIVLSSFEEFEYVRSTFHSGVADYILKP 106 >BH3679 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 257 Score = 88.6 bits (218), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 40/98 (40%), Positives = 60/98 (61%) Query: 424 ITECLSWIREHTKEEVTLERAADYFHFNPSYFSTLFKSRTGRTFSEHVTAVRMKRAKELL 483 I E L +I H +++ L+ A H NPSY S LFK +T TFSE++T R++ AK LL Sbjct: 156 IHEALHYIDAHLDQKIGLKDVAKSVHLNPSYLSALFKEKTNMTFSEYLTRKRLQHAKHLL 215 Query: 484 AEDKLRIYEISVECGFQDPKYFCRVFKKYHSMSPKTYK 521 I +I+ +CG+Q KYF ++FK++ M+P Y+ Sbjct: 216 VSTDRPISDIAEKCGYQTAKYFIKIFKEHEGMTPTQYR 253 Score = 75.9 bits (185), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 44/113 (38%), Positives = 63/113 (55%), Gaps = 1/113 (0%) Query: 3 ILIVDDEPRHLRGMVNLIHRLRPED-QVAVAKDGLAAMELAKSHRPEAILTDIRMPGMDG 61 +LIVDDEPR RG+ + P+ V A A+ + I+TDIRMP M G Sbjct: 6 VLIVDDEPRSRRGLKRTLEAWSPKLLNVQTAASAKEAITRLNEETIDLIITDIRMPEMSG 65 Query: 62 LEFLEWLKLEGIKAKVVMVSAYNLFEYAQTAVRHGAYDYLLKPVDVQKIEEVL 114 L ++ L+ + K +++SAY+ FEYA TA+ G YLLKPV +K+ E + Sbjct: 66 LHLVKALQEQNPKPVCIVISAYSEFEYAHTALELGVRHYLLKPVKKEKLIEAV 118 >BH3842 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 530 Score = 82.8 bits (203), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 39/93 (41%), Positives = 59/93 (63%) Query: 429 SWIREHTKEEVTLERAADYFHFNPSYFSTLFKSRTGRTFSEHVTAVRMKRAKELLAEDKL 488 ++ +H +E +TL+ A + +P Y S LFK R TFSE+VT +R++ AK+LL+ + Sbjct: 422 DYVHDHFREPLTLDDLAKHIGLSPYYVSKLFKERFDLTFSEYVTQLRVEHAKQLLSLTQK 481 Query: 489 RIYEISVECGFQDPKYFCRVFKKYHSMSPKTYK 521 + EI+ E G++DP YF RVFKK SP Y+ Sbjct: 482 SLKEITYEIGYKDPNYFSRVFKKLTGKSPSDYR 514 Score = 78.2 bits (191), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 43/118 (36%), Positives = 72/118 (61%), Gaps = 2/118 (1%) Query: 1 VRILIVDDEPRHLRGMVNLIHRLRPEDQ-VAVAKDGLAAMELAKSHRPEAILTDIRMPGM 59 + +LIVDDE G+ L + RP V A +G A+E A +P+ ++ DI+MPG+ Sbjct: 2 INVLIVDDEQIEREGL-KLFLKDRPNIHIVGEASNGRQAIEKALETQPDLMMMDIKMPGV 60 Query: 60 DGLEFLEWLKLEGIKAKVVMVSAYNLFEYAQTAVRHGAYDYLLKPVDVQKIEEVLSRI 117 DG++ ++ +K + + + +MVSA++ FEYA+ +R+G +YLLKP + + L RI Sbjct: 61 DGVQAVKEIKQQLPETEFIMVSAFDTFEYAREVMRYGVKEYLLKPTTKEDVLTALDRI 118 >Z3647 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 285 Score = 82.0 bits (201), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 44/152 (28%), Positives = 77/152 (50%), Gaps = 3/152 (1%) Query: 372 LDRKAGQMLADTATRLIRSC---SSYKELMARLAKALQEVQLALSLSRQDQGEVVITECL 428 L R G+ + T L RS + + L+ L + V + Q +G+ + Sbjct: 128 LQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSLLHLCRNVVNMPPGNSQPRGDFLYHSIC 187 Query: 429 SWIREHTKEEVTLERAADYFHFNPSYFSTLFKSRTGRTFSEHVTAVRMKRAKELLAEDKL 488 +W++++ + +T E A +F+ P++ S LF F E+V VRM +A+ +L + L Sbjct: 188 NWVQDNYAQPLTRESVAQFFNITPNHLSKLFAQHGTMRFIEYVRWVRMAKARMILQKYHL 247 Query: 489 RIYEISVECGFQDPKYFCRVFKKYHSMSPKTY 520 I+E++ CGF D YFCRVF++ ++P Y Sbjct: 248 SIHEVAQRCGFPDSDYFCRVFRRQFGLTPGEY 279 >BH2109 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 525 Score = 81.3 bits (199), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 59/257 (22%), Positives = 122/257 (47%), Gaps = 9/257 (3%) Query: 274 LAGAPQAYLSARMAYSYNFYEGWKGLVFLDEISSSPLAASPDWGR-----LYEALKGDDA 328 L+ ++Y +A +Y + K VF+ E + S + L E ++ +++ Sbjct: 270 LSTIKKSYETANSLRNYKYIFNEKN-VFIHEQTESVDTTYDELNNHLFVTLMEQMEENNS 328 Query: 329 ALVLHVCGEIFTQLAGGGHAVPMLLKEKASLMLLQIRSDNQDILDRKAGQMLADTATRLI 388 ++++ +IF++ VP +K + + ++ Q ++ G++++ A Sbjct: 329 DRIVNIIDKIFSEFQTKKF-VPSAVKTSVNRCVHKVMQTVQ-AMEGDTGKIISAQAMTNW 386 Query: 389 RSCS-SYKELMARLAKALQEVQLALSLSRQDQGEVVITECLSWIREHTKEEVTLERAADY 447 S + ++ +L K + E + + R++ + I + ++I H E ++L+ A Sbjct: 387 ESYNLTFDQLKQVFTKFVLEAAVIIQELRKENMKSDIYKVKTYIENHYHENISLKSIASL 446 Query: 448 FHFNPSYFSTLFKSRTGRTFSEHVTAVRMKRAKELLAEDKLRIYEISVECGFQDPKYFCR 507 F+ NP Y LFK G F E++ +RM AK+LL + +R+YE++ GF YF Sbjct: 447 FYMNPVYMGQLFKKTFGVYFKEYLLKLRMNEAKKLLRQTDMRVYEVAERVGFGSTDYFVT 506 Query: 508 VFKKYHSMSPKTYKHVL 524 F+K + M+P Y++ L Sbjct: 507 QFEKINKMTPTEYRNKL 523 Score = 80.1 bits (196), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 44/123 (35%), Positives = 73/123 (59%), Gaps = 2/123 (1%) Query: 2 RILIVDDEPRHLRGMVNLIHRLRPEDQVAV-AKDGLAAMELAKSHRPEAILTDIRMPGMD 60 ++L+ DDE G+ +LI + +VA A +G A +L + +P+ ++TDIRMP D Sbjct: 3 KVLLADDEFFVRHGLKSLIEWEKCGYEVAAEADNGEDAFKLVQEIKPDLVITDIRMPVFD 62 Query: 61 GLEFLEWLKLE-GIKAKVVMVSAYNLFEYAQTAVRHGAYDYLLKPVDVQKIEEVLSRIDV 119 GL+ ++ + K +++S YN F YAQ AVR GA D++LKP+D ++E L ++ Sbjct: 63 GLQLIQKVNETLSFNTKFIIISGYNDFSYAQKAVRFGAVDFVLKPIDQDELEAALIKLAA 122 Query: 120 QLN 122 L+ Sbjct: 123 TLD 125 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,650,974 Number of extensions: 1404559 Number of successful extensions: 4539 Number of sequences better than 1.0e-05: 308 Number of HSP's gapped: 4609 Number of HSP's successfully gapped: 320 Length of query: 531 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 420 Effective length of database: 25,576,357 Effective search space: 10742069940 Effective search space used: 10742069940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)