BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0437.0011.p (346 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ydjJ [ER] COG1063 Threonine dehydrogenase and related Zn-depende... 318 8e-87 ECs2483 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 314 1e-85 BS_gutB [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 291 9e-79 YJR159w [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 245 6e-65 YDL246c [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 244 1e-64 >ydjJ [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 347 Score = 318 bits (815), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 169/350 (48%), Positives = 224/350 (64%), Gaps = 10/350 (2%) Query: 1 MKNRAFYMTDLKKLEMNEIGMPKAKEGEVIVKLEYVGICGSDVHYLEHGRIGDFVVEGD- 59 MKN + +++ +P KE EV++K+EYVGICGSDVH E G F+ D Sbjct: 1 MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESG---PFIPPKDP 57 Query: 60 ---FILGHECAGAVVELGPGVKNLKVGDRVALEPGVTCGQCEFCKSGKYNLCPDVKFLAT 116 LGHECAG VV +G V+ K GDRV +EPGV CG C +C GKYN+CPDV F+AT Sbjct: 58 NQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMAT 117 Query: 117 PP-YHGCLMDYIAFPENMAFKLPDNVSSEEGALVEPLAVGLHAAAQGGIKLGDRVVILGA 175 P Y G L Y+ PE+ +KLPDN+ + EGALVEP AVG+HAA +K G +++ILGA Sbjct: 118 QPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHAAMLADVKPGKKIIILGA 177 Query: 176 GCIGLVTLLACKAYGATDIVVVDIIEKRLEAAKRLGATRVINAKQENVRDVISLLTDGQG 235 GCIGL+TL ACK GAT+I VVD++EKRL A++LGAT VIN +E+ T+ G Sbjct: 178 GCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVINGAKEDTIARCQQFTEDMG 237 Query: 236 MDKVIETAGSEHTVKQTPYLVKRGGTIVLVGLAAKDIIDFDFMQIMFKEADIKSVFRYRN 295 D V ETAGS TVKQ PYLV RGG I++VG D +F++I +E I++VFRY N Sbjct: 238 ADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSA-INFLKIN-REVTIQTVFRYAN 295 Query: 296 LYPXXXXXXXXXXXXVKGIVTHEFSFEDTQKAFDYVIDNKQEVVKAVIRM 345 YP VK +VTH + + D Q+AF+ ++NK++++K VI++ Sbjct: 296 RYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNNKRDIIKGVIKI 345 >ECs2483 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 347 Score = 314 bits (805), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 167/350 (47%), Positives = 222/350 (63%), Gaps = 10/350 (2%) Query: 1 MKNRAFYMTDLKKLEMNEIGMPKAKEGEVIVKLEYVGICGSDVHYLEHGRIGDFVVEGD- 59 MKN + +++ +P KE EV++K+EYVGICGSDVH E G F+ D Sbjct: 1 MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESG---PFIPPKDP 57 Query: 60 ---FILGHECAGAVVELGPGVKNLKVGDRVALEPGVTCGQCEFCKSGKYNLCPDVKFLAT 116 LGHECAG VV +G V+ K GDRV +EPGV CG C +C GKYN+CPDV F+AT Sbjct: 58 NQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMAT 117 Query: 117 PP-YHGCLMDYIAFPENMAFKLPDNVSSEEGALVEPLAVGLHAAAQGGIKLGDRVVILGA 175 P Y G L Y+ PE+ +KLPDN+ + EG LVEP AVG+HAA +K G +++ILGA Sbjct: 118 QPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHAAMLADVKPGKKIIILGA 177 Query: 176 GCIGLVTLLACKAYGATDIVVVDIIEKRLEAAKRLGATRVINAKQENVRDVISLLTDGQG 235 GCIGL+TL ACK GAT+I VVD++EKRL A++LGAT VIN +E+ T+ G Sbjct: 178 GCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVINGAKEDTIARCQQFTEDMG 237 Query: 236 MDKVIETAGSEHTVKQTPYLVKRGGTIVLVGLAAKDIIDFDFMQIMFKEADIKSVFRYRN 295 D V ETAGS TVKQ PYLV RGG I++VG +F++I +E I++VFRY N Sbjct: 238 ADIVFETAGSAVTVKQAPYLVMRGGKIMIVG-TVPGASAINFLKIN-REVTIQTVFRYAN 295 Query: 296 LYPXXXXXXXXXXXXVKGIVTHEFSFEDTQKAFDYVIDNKQEVVKAVIRM 345 YP VK +VTH + + D Q+AF+ ++NK++++K VI++ Sbjct: 296 RYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNNKRDIIKGVIKI 345 >BS_gutB [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 353 Score = 291 bits (745), Expect = 9e-79, Method: Compositional matrix adjust. Identities = 146/340 (42%), Positives = 219/340 (64%) Query: 4 RAFYMTDLKKLEMNEIGMPKAKEGEVIVKLEYVGICGSDVHYLEHGRIGDFVVEGDFILG 63 +A M + +++++ + +P EV++K+ VGICGSD+HY +GRIG++VVE FILG Sbjct: 10 KAAVMHNTREIKIETLPVPDINHDEVLIKVMAVGICGSDLHYYTNGRIGNYVVEKPFILG 69 Query: 64 HECAGAVVELGPGVKNLKVGDRVALEPGVTCGQCEFCKSGKYNLCPDVKFLATPPYHGCL 123 HECAG + +G V KVGDRVA+EPGVTCG+CE CK G+YNLCPDV+FLATPP G Sbjct: 70 HECAGEIAAVGSSVDQFKVGDRVAVEPGVTCGRCEACKEGRYNLCPDVQFLATPPVDGAF 129 Query: 124 MDYIAFPENMAFKLPDNVSSEEGALVEPLAVGLHAAAQGGIKLGDRVVILGAGCIGLVTL 183 + YI ++ F +PD++S EE AL+EP +VG+HAAA+ ++ G + I+G G +GL+ + Sbjct: 130 VQYIKMRQDFVFLIPDSLSYEEAALIEPFSVGIHAAARTKLQPGSTIAIMGMGPVGLMAV 189 Query: 184 LACKAYGATDIVVVDIIEKRLEAAKRLGATRVINAKQENVRDVISLLTDGQGMDKVIETA 243 A KA+GA I+V D+ RLEAAK++GAT +IN ++++ + I +T+ +G+D ETA Sbjct: 190 AAAKAFGAGTIIVTDLEPLRLEAAKKMGATHIINIREQDALEEIKTITNDRGVDVAWETA 249 Query: 244 GSEHTVKQTPYLVKRGGTIVLVGLAAKDIIDFDFMQIMFKEADIKSVFRYRNLYPXXXXX 303 G+ ++ V+RGG + +VGL +++ I + I E DI +FRY N YP Sbjct: 250 GNPAALQSALASVRRGGKLAIVGLPSQNEIPLNVPFIADNEIDIYGIFRYANTYPKGIEF 309 Query: 304 XXXXXXXVKGIVTHEFSFEDTQKAFDYVIDNKQEVVKAVI 343 K +VT ++S E TQ A + + K E +K ++ Sbjct: 310 LASGIVDTKHLVTDQYSLEQTQDAMERALQFKNECLKVMV 349 >YJR159w [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 357 Score = 245 bits (626), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 134/350 (38%), Positives = 200/350 (57%), Gaps = 9/350 (2%) Query: 2 KNRAFYMTDLKKLEMNEIGMPKAKEGEVI-VKLEYVGICGSDVHYLEHGRIGDFVVEGDF 60 N A + + + + + +P K+ + + ++ GICGSD+HY G IG ++++ Sbjct: 5 SNPAVVLEKVGDIAIEQRPIPTIKDPHYVKLAIKATGICGSDIHYYRSGGIGKYILKAPM 64 Query: 61 ILGHECAGAVVELGPGVKNLKVGDRVALEPGVTCGQCEFCKSGKYNLCPDVKFLATPPYH 120 +LGHE +G VVE+G V +KVGDRVA+EPGV + K G+YNLCP + F ATPP Sbjct: 65 VLGHESSGQVVEVGDAVTRVKVGDRVAIEPGVPSRYSDETKEGRYNLCPHMAFAATPPID 124 Query: 121 GCLMDYIAFPENMAFKLPDNVSSEEGALVEPLAVGLHAAAQGGIKLGDRVVILGAGCIGL 180 G L+ Y PE+ KLP+ VS EEGA VEPL+VG+H+ G++ G +VV+ GAG +GL Sbjct: 125 GTLVKYYLSPEDFLVKLPEGVSYEEGACVEPLSVGVHSNKLAGVRFGTKVVVFGAGPVGL 184 Query: 181 VTLLACKAYGATDIVVVDIIEKRLEAAKRLGATRVINAKQ------ENVRDVISLLTDGQ 234 +T +A+GATD++ VD+ + +L+ AK GAT N+ Q +++ D + L G Sbjct: 185 LTGAVARAFGATDVIFVDVFDNKLQRAKDFGATNTFNSSQFSTDKAQDLADGVQKLLGGN 244 Query: 235 GMDKVIETAGSEHTVKQTPYLVKRGGTIVLVGLAAKDIIDFDFMQIMFKEADIKSVFRYR 294 D V E +G++ + K GGT+V VG+ K+ +F ++ KE + FRY Sbjct: 245 HADVVFECSGADVCIDAAVKTTKVGGTMVQVGM-GKNYTNFPIAEVSGKEMKLIGCFRYS 303 Query: 295 -NLYPXXXXXXXXXXXXVKGIVTHEFSFEDTQKAFDYVIDNKQEVVKAVI 343 Y VK ++TH+F FED KA+DY I + EVVK +I Sbjct: 304 FGDYRDAVNLVATGKVNVKPLITHKFKFEDAAKAYDYNIAHGGEVVKTII 353 >YDL246c [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 311 Score = 244 bits (624), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 0/350 (0%), Positives = 0/350 (0%), Gaps = 9/350 (2%) Query: 2 KNRAFYMTDLKKLEMNEIGMPKAKEGEVI-VKLEYVGICGSDVHYLEHGRIGDFVVEGDF 60 + + + + + V + + + +G + + Sbjct: 5 FFKHLLESLKNLKRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVAYM 64 Query: 61 ILGHECAGAVVELGPGVKNLKVGDRVALEPGVTCGQCEFCKSGKYNLCPDVKFLATPPYH 120 L ++ K + +K L + Sbjct: 65 KLDVHDQETKIQDPKDNKKTINNPDHLKIYDSIKKVPNVKTVEFSSKEAQLKELTKALGN 124 Query: 121 GCLMDYIAFPENMAFKLPDNVSSEEGALVEPLAVGLHAAAQGGIKLGDRVVILGAGCIGL 180 + + + V L + + + Sbjct: 125 NWNLFKGDANPLYDAYIVEADKPANVKSVTSKISKLSGIEKATYGGANTAKLFQIAQGIR 184 Query: 181 VTLLACKAYGATDIVVVDIIEKRLEAAKRLGATRVINAKQ------ENVRDVISLLTDGQ 234 A V + R+ R +++ + Sbjct: 185 TWGTAAAILLIFVAVFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFFLEGAWVGLL 244 Query: 235 GMDKVIETAGSEHTVKQTPYLVKRGGTIVLVGLAAKDIIDFDFMQIMFKEADIKSVFRYR 294 G + + + + K+ I + I S Sbjct: 245 GSIIPGLIVAWAYRLAFVSLTPSLQSQKLAMFA-PKEFIPAIVGLMAVIGILIGSFGALL 303 Query: 295 -NLYPXXXXXXXXXXXXVKGIVTHEFSFEDTQKAFDYVIDNKQEVVKAVI 343 Sbjct: 304 S 353 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.141 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,791,204 Number of extensions: 1060034 Number of successful extensions: 4816 Number of sequences better than 1.0e-05: 276 Number of HSP's gapped: 4318 Number of HSP's successfully gapped: 282 Length of query: 346 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 239 Effective length of database: 26,037,693 Effective search space: 6223008627 Effective search space used: 6223008627 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)