BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0437.0003.p (250 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1655 [LKJ] COG0513 Superfamily II DNA and RNA helicases 183 2e-46 TM1655 [TK] COG0745 Response regulators consisting of a CheY-lik... 183 2e-46 SA0017 [TK] COG0745 Response regulators consisting of a CheY-lik... 181 9e-46 Cgl0399 [TK] COG0745 Response regulators consisting of a CheY-li... 180 2e-45 CAC0564 [TK] COG0745 Response regulators consisting of a CheY-li... 176 2e-44 >STM1655 [LKJ] COG0513 Superfamily II DNA and RNA helicases Length = 457 Score = 183 bits (465), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 20/238 (8%), Positives = 43/238 (18%), Gaps = 8/238 (3%) Query: 9 GGEKTMNDTILIIEDEAGIACMLELLLSREGYSSVLTASTGAEAMSLLDSREPDIILLDV 68 + L E G M + + + +A + + L Sbjct: 5 STLNVLPAAQLNNLTELGYLEMTPVQAAALPVI-LAGNDVRVQARTGSGKTAAFGLGLLH 63 Query: 69 MLPDCNGFELCLELR---KKTNAPVLFLTSRAADYDKLTGFAMGGDDYITKPFNSLEVVA 125 + L L + + L A + G +SL+ Sbjct: 64 RIDVTLFQTQALVLCPTRELADQVAGELRRLARFLPNTKILTLCGGQPFGAQRDSLQHAP 123 Query: 126 RIKA---QLARKRLYQQEAPAACYAFADFEISKDTGGLTVRGQQVHCTAKEFALLLFFAE 182 I L ++ D + + Sbjct: 124 HIIVATPGRLLDHLQKETVSLDALH-ILVMDEADRMLDMGFSDAIDEVIRFAPATRQTLL 182 Query: 183 HPGRLFTTAQLYEQVWGQMQLGDEKTVVIYISKVRNKIERDPKQPRYIVNFRGLGYKF 240 Q + E V + + + + + L Sbjct: 183 FSATWPEAIAAISGRVQQQPIRIEIDTVDALPAIEQQFFETSAHEKISLLQTLLSQHQ 240 >TM1655 [TK] COG0745 Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain Length = 247 Score = 183 bits (465), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 95/238 (39%), Positives = 148/238 (62%), Gaps = 8/238 (3%) Query: 9 GGEKTMNDTILIIEDEAGIACMLELLLSREGYSSVLTASTGAEAMSLLDSREPDIILLDV 68 GG K IL+++D+ I ++ LS+EGY VL A G EA+ + + + D+ ++D+ Sbjct: 5 GGSKMAKKKILVVDDDPAILELVGYNLSKEGYE-VLKAYDGEEALKIANDEDVDMFIVDI 63 Query: 69 MLPDCNGFELCLELR---KKTNAPVLFLTSRAADYDKLTGFAMGGDDYITKPFNSLEVVA 125 MLP +GFEL ++R K N PV+FL+++ ++DK+ G +G DDYITKPF+ E++A Sbjct: 64 MLPGIDGFELVRKIRSMEKYKNTPVIFLSAKGEEFDKVLGLELGADDYITKPFSVRELLA 123 Query: 126 RIKA---QLARKRLYQQEAPAACYAFADFEISKDTGGLTVRGQQVHCTAKEFALLLFFAE 182 R+KA +L+ ++E P A D EI + + VRG++V+ T EF LL F AE Sbjct: 124 RVKAIFRRLSTATQSKEERPKKIIA-KDLEIDVEKYEVKVRGKKVNLTPLEFELLRFLAE 182 Query: 183 HPGRLFTTAQLYEQVWGQMQLGDEKTVVIYISKVRNKIERDPKQPRYIVNFRGLGYKF 240 + G++F+ L +++WG GD +TV ++I ++R KIE DP P+YI+ RG GYKF Sbjct: 183 NEGKVFSRDVLLDKLWGYDYYGDTRTVDVHIRRLRTKIEEDPSNPKYIITVRGKGYKF 240 >SA0017 [TK] COG0745 Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain Length = 235 Score = 181 bits (459), Expect = 9e-46, Method: Compositional matrix adjust. Identities = 92/230 (40%), Positives = 137/230 (59%), Gaps = 8/230 (3%) Query: 14 MNDTILIIEDEAGIACMLELLLSREGYSSVLTASTGAEAMSLLDSREPDIILLDVMLPDC 73 M +++++DE IA +LE L +EGY V A G +A+ L+ EPDI+LLD+MLP Sbjct: 3 MARKVVVVDDEKPIADILEFNLKKEGYD-VYCAYDGNDAVDLIYEEEPDIVLLDIMLPGR 61 Query: 74 NGFELCLELRKKTNAPVLFLTSRAADYDKLTGFAMGGDDYITKPFNSLEVVARIKAQLAR 133 +G E+C E+RKK P++ LT++ ++ DK+ G +G DDY+TKPF++ E++AR+KA L Sbjct: 62 DGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTKPFSTRELIARVKANL-- 119 Query: 134 KRLYQQEAPAAC-----YAFADFEISKDTGGLTVRGQQVHCTAKEFALLLFFAEHPGRLF 188 +R Y Q A D I D + RG+ + T +EF L + ++H G++ Sbjct: 120 RRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTHREFELFHYLSKHMGQVM 179 Query: 189 TTAQLYEQVWGQMQLGDEKTVVIYISKVRNKIERDPKQPRYIVNFRGLGY 238 T L + VWG GD +TV + I ++R KIE DP P YIV RG+GY Sbjct: 180 TREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEYIVTRRGVGY 229 >Cgl0399 [TK] COG0745 Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain Length = 232 Score = 180 bits (457), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 91/228 (39%), Positives = 142/228 (62%), Gaps = 6/228 (2%) Query: 18 ILIIEDEAGIACMLELLLSREGYSSVLTASTGAEAMSLLDSREPDIILLDVMLPDCNGFE 77 ILI+EDE +A L LL +EG+ +++ A G A+ E DI+LLD+MLP +G + Sbjct: 4 ILIVEDEESLADPLAFLLRKEGFDTII-AGDGPTALVEFSRNEIDIVLLDLMLPGMSGTD 62 Query: 78 LCLELRKKTNAPVLFLTSRAADYDKLTGFAMGGDDYITKPFNSLEVVARIKAQLARKRLY 137 +C ELR + PV+ +T+R ++ DK+ G +G DDY+TKP++S E++ARI+A L R+ + Sbjct: 63 VCKELRSVSTVPVIMVTARDSEIDKVVGLELGADDYVTKPYSSRELIARIRAVLRRRGVT 122 Query: 138 QQEAPAACYAFADFE-----ISKDTGGLTVRGQQVHCTAKEFALLLFFAEHPGRLFTTAQ 192 + EA E + D+ +TV G+ V KEF LL + + GR+ T Q Sbjct: 123 ETEAEELPLDDQILEGGRVRMDVDSHTVTVGGEPVSMPLKEFDLLEYLLRNAGRVLTRGQ 182 Query: 193 LYEQVWGQMQLGDEKTVVIYISKVRNKIERDPKQPRYIVNFRGLGYKF 240 L +++WG +GD KT+ +++ ++R+KIE +P +PRY+V RGLGYKF Sbjct: 183 LIDRIWGADYVGDTKTLDVHVKRLRSKIEEEPSRPRYLVTVRGLGYKF 230 >CAC0564 [TK] COG0745 Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain Length = 233 Score = 176 bits (447), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 80/226 (35%), Positives = 140/226 (61%), Gaps = 3/226 (1%) Query: 17 TILIIEDEAGIACMLELLLSREGYSSVLTASTGAEAMSLLDSREPDIILLDVMLPDCNGF 76 T+L+++DE I ++ + L+ EG++ V+ A G EA+ +L E ++LLD+M+P +G Sbjct: 5 TVLVVDDEKEIRDLIGIYLNNEGFN-VIKAENGVEALEILKDTEVQLVLLDIMMPKMDGI 63 Query: 77 ELCLELRKKTNAPVLFLTSRAADYDKLTGFAMGGDDYITKPFNSLEVVARIKAQLAR--K 134 C+++R+ N P++ L+++ D DK+ G G DDYI+KPFN LE++AR+K+Q+ R K Sbjct: 64 TTCMKIREDKNTPIIMLSAKGEDMDKILGLTTGADDYISKPFNPLELIARVKSQIRRYTK 123 Query: 135 RLYQQEAPAACYAFADFEISKDTGGLTVRGQQVHCTAKEFALLLFFAEHPGRLFTTAQLY 194 E+ +A + I+ DT + + + V T +EF +L+ + + G +F+T ++Y Sbjct: 124 LNSTYESNSANIEVGEITINTDTHEVKIGDRTVSLTKREFDILVLLSRNKGVVFSTEKIY 183 Query: 195 EQVWGQMQLGDEKTVVIYISKVRNKIERDPKQPRYIVNFRGLGYKF 240 E VW + TV+++I K+R KIE++P++P YI G+GYK Sbjct: 184 ESVWHEEFYDCHNTVMVHIRKIREKIEKNPRKPEYIKTVWGVGYKI 229 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,306,512 Number of extensions: 716881 Number of successful extensions: 2409 Number of sequences better than 1.0e-05: 434 Number of HSP's gapped: 1913 Number of HSP's successfully gapped: 434 Length of query: 250 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 146 Effective length of database: 26,383,695 Effective search space: 3852019470 Effective search space used: 3852019470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)